JPS6486871A - Method for separating and purifying recombinant protein - Google Patents
Method for separating and purifying recombinant proteinInfo
- Publication number
- JPS6486871A JPS6486871A JP24701787A JP24701787A JPS6486871A JP S6486871 A JPS6486871 A JP S6486871A JP 24701787 A JP24701787 A JP 24701787A JP 24701787 A JP24701787 A JP 24701787A JP S6486871 A JPS6486871 A JP S6486871A
- Authority
- JP
- Japan
- Prior art keywords
- microbial cells
- chromatography
- escherichia coli
- bacillus subtilis
- recombinant plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 230000000813 microbial effect Effects 0.000 abstract 3
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 2
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 229960002385 streptomycin sulfate Drugs 0.000 abstract 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 101900343733 Bacillus subtilis Dihydrofolate reductase Proteins 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000004440 column chromatography Methods 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000001641 gel filtration chromatography Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24701787A JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24701787A JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6486871A true JPS6486871A (en) | 1989-03-31 |
| JPH0333315B2 JPH0333315B2 (enrdf_load_html_response) | 1991-05-16 |
Family
ID=17157159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24701787A Granted JPS6486871A (en) | 1987-09-30 | 1987-09-30 | Method for separating and purifying recombinant protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6486871A (enrdf_load_html_response) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100401517C (zh) | 2000-07-19 | 2008-07-09 | 新藤电子工业株式会社 | 半导体装置 |
-
1987
- 1987-09-30 JP JP24701787A patent/JPS6486871A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0333315B2 (enrdf_load_html_response) | 1991-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2046177T3 (es) | Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas. | |
| AU4067985A (en) | Hosts and methods for producing recombinant products in high yields | |
| AU4251989A (en) | Method for the purification of proteins | |
| NO910797L (no) | Dna-fragment som koder for et polypeptid med nitrilhydratase-aktivitet, en transformant inneholdende genet og en fremgangsmaate for fremstilling av amider ved bruk av transformanten. | |
| AU1049788A (en) | Methods and peptides for the treatment of non-ige-mediated diseases | |
| SE8402861D0 (sv) | Rening av biologiskt material | |
| IL80960A0 (en) | Process for improving the expression of eukaryotic proteins | |
| EP0263518A3 (en) | Purification of recombinant tumor necrosis factor | |
| EP0269455A3 (en) | Highly purified fused protein comprising human ige fc fragment and production thereof | |
| AU551490B2 (en) | Dna code for protein to suppress abnormal protein degradation | |
| Hawkins et al. | Thiol-protein disulphide oxidoreductases. Differences between protein disulphide-isomerase and glutathione-insulin transhydrogenase activities in ox liver | |
| JPS6486871A (en) | Method for separating and purifying recombinant protein | |
| EP0159943A3 (en) | Expression vectors, dna fragments, peptides, hosts, and process for production of proteins | |
| Rappaport et al. | Non phosphorylation in vitro of the 6 S tubulin from brain and thyroid tissue | |
| Nakata et al. | Simple and rapid purification of tryptophan 5-monooxygenase from rabbit brain by affinity chromatography | |
| Hillson et al. | Bovine liver thiol-protein disulphide oxidoreductases. An alternative method for differential purification and resolution of protein disulphide-isomerase and glutathione-insulin transhydrogenase | |
| LTIP1543A (en) | Process for enzymatic decomposition of recombinant proteins by means of iga-protease, fused protein, recombinant dna, cell | |
| KR970001813B1 (ko) | 삼상분할법을 이용한 아미노펩티다제의 정제방법 | |
| AU590712B2 (en) | Immune interferon and method for its purification | |
| ATE154074T1 (de) | Verfahren zur herstellung und reinigung des carboxyterminalen fragments der dna-polymerase i von streptococcus pneumoniae | |
| YUTSUDO et al. | PURIFICATION AND SOME PROPERTIES OF SHIGA TOXIN PRODUCED BY SHIGELLA DYSENTERIAE | |
| KR980002265A (ko) | 신규한 플라스미드 pKLE와 이를 이용한 리파제의 대량생산방법 | |
| FR2424319A1 (fr) | Procede de preparation de cytochrome c au moyen d'une levure | |
| Naumov | Chromatographic purification of plasmid DNA. | |
| Sa et al. | Purification and Properties of Thioltransferase from Leaf Mustard |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |