JPS6455180A - Production of proline dipeptidase - Google Patents

Production of proline dipeptidase

Info

Publication number
JPS6455180A
JPS6455180A JP21406787A JP21406787A JPS6455180A JP S6455180 A JPS6455180 A JP S6455180A JP 21406787 A JP21406787 A JP 21406787A JP 21406787 A JP21406787 A JP 21406787A JP S6455180 A JPS6455180 A JP S6455180A
Authority
JP
Japan
Prior art keywords
vol
proline
trichosporon
culture medium
pseudomonas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP21406787A
Other languages
Japanese (ja)
Other versions
JPH0775540B2 (en
Inventor
Kumiko Yagi
Sadao Kageyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daicel Corp
Original Assignee
Daicel Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daicel Chemical Industries Ltd filed Critical Daicel Chemical Industries Ltd
Priority to JP21406787A priority Critical patent/JPH0775540B2/en
Publication of JPS6455180A publication Critical patent/JPS6455180A/en
Publication of JPH0775540B2 publication Critical patent/JPH0775540B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To efficiently obtain the titled enzyme useful for producing a blended amino acid substance, etc., by cultivating a specific bacterium belonging to the genus Pseudomonas or Trichosporon in a nutrient culture medium. CONSTITUTION:Pseudomonas.fluorescens IFO12055 or Trichosporon sp. 107-1 (FERM P-9489) is cultivated in a culture medium containing 0.5-2% (wt./vol.) carbon source, 0.3-1% (wt./vol.) nitrogen source, 0.05-0.1% (wt./vol.) magnesium, 0.001-0.003% (wt./vol.) calcium, manganese and ferric ion and 0.5-1% (wt./vol.) glycyl-L-proline, polypeptone, etc., at pH5-8 and 20-40 deg.C for 18-40hr. Microbial cells are then collected from the resultant culture and crushed to isolate the aimed proline dipeptidase from the obtained crude extract solution.
JP21406787A 1987-08-27 1987-08-27 Method for producing proline dipeptidase Expired - Lifetime JPH0775540B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP21406787A JPH0775540B2 (en) 1987-08-27 1987-08-27 Method for producing proline dipeptidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP21406787A JPH0775540B2 (en) 1987-08-27 1987-08-27 Method for producing proline dipeptidase

Publications (2)

Publication Number Publication Date
JPS6455180A true JPS6455180A (en) 1989-03-02
JPH0775540B2 JPH0775540B2 (en) 1995-08-16

Family

ID=16649703

Family Applications (1)

Application Number Title Priority Date Filing Date
JP21406787A Expired - Lifetime JPH0775540B2 (en) 1987-08-27 1987-08-27 Method for producing proline dipeptidase

Country Status (1)

Country Link
JP (1) JPH0775540B2 (en)

Also Published As

Publication number Publication date
JPH0775540B2 (en) 1995-08-16

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