JPS6455180A - Production of proline dipeptidase - Google Patents
Production of proline dipeptidaseInfo
- Publication number
- JPS6455180A JPS6455180A JP21406787A JP21406787A JPS6455180A JP S6455180 A JPS6455180 A JP S6455180A JP 21406787 A JP21406787 A JP 21406787A JP 21406787 A JP21406787 A JP 21406787A JP S6455180 A JPS6455180 A JP S6455180A
- Authority
- JP
- Japan
- Prior art keywords
- vol
- proline
- trichosporon
- culture medium
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To efficiently obtain the titled enzyme useful for producing a blended amino acid substance, etc., by cultivating a specific bacterium belonging to the genus Pseudomonas or Trichosporon in a nutrient culture medium. CONSTITUTION:Pseudomonas.fluorescens IFO12055 or Trichosporon sp. 107-1 (FERM P-9489) is cultivated in a culture medium containing 0.5-2% (wt./vol.) carbon source, 0.3-1% (wt./vol.) nitrogen source, 0.05-0.1% (wt./vol.) magnesium, 0.001-0.003% (wt./vol.) calcium, manganese and ferric ion and 0.5-1% (wt./vol.) glycyl-L-proline, polypeptone, etc., at pH5-8 and 20-40 deg.C for 18-40hr. Microbial cells are then collected from the resultant culture and crushed to isolate the aimed proline dipeptidase from the obtained crude extract solution.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP21406787A JPH0775540B2 (en) | 1987-08-27 | 1987-08-27 | Method for producing proline dipeptidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP21406787A JPH0775540B2 (en) | 1987-08-27 | 1987-08-27 | Method for producing proline dipeptidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6455180A true JPS6455180A (en) | 1989-03-02 |
JPH0775540B2 JPH0775540B2 (en) | 1995-08-16 |
Family
ID=16649703
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP21406787A Expired - Lifetime JPH0775540B2 (en) | 1987-08-27 | 1987-08-27 | Method for producing proline dipeptidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0775540B2 (en) |
-
1987
- 1987-08-27 JP JP21406787A patent/JPH0775540B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JPH0775540B2 (en) | 1995-08-16 |
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