JPS6383017A - Anti-suntan cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having anti-suntan effect and effective in protecting skin from sunlight, by using baicalin, senega saponin, etc., as a component optionally in combination with astaxanthin, beta-carotene, etc. CONSTITUTION:The objective cosmetic contains a compound selected from baicalin, baicalein, senega saponin and onjisaponin or contains the above compound and a compound selected from astaxanthin, beta-carotene and shikonin. Ultraviolet rays (UV-A), (UV-B) can be absorbed and shielded on the cuticle by the application of the cosmetic. It is also effective in absorbing and shielding light extending over a wide wavelength range from ultraviolet ray to visible light.

Description

DETAILED DESCRIPTION OF THE INVENTION (A) Object of the Invention The present invention relates to a coating composition that protects the skin from sunlight and is formulated with extracts (ingredients) derived from animals and plants.

(Industrial Application) The present invention provides a skin-application paint that has a sunburn prevention effect;
Skin + = Protects (skin) from sunlight (sunrays) and can be used as cosmetics and external medicines.

(Prior art) In the formulation of sunburn preventive agents, sunburn: UV
-B (300 to 320 nm) wavelength light, suntan: UV-A (320 to 400 nm) wavelength light rays are absorbed and blocked on the epidermis.2 Substances are used.

As a representative example [1], this can be investigated from the prescription of external rubs such as VT products.

There is PABA (para-aminoben, rho-aroic acid).

Since PABA has maximum absorption in the vicinity of ultraviolet rays of 270 to 320 nm, it has been used as an agent to prevent sunburn, especially when accompanied by erythema. In other words, PABA.

It absorbs and blocks light rays near sunburn on the epidermis, thereby suppressing erythema. However, it is known that 320-400 nm light called suntan does not directly cause erythema but darkens the epidermis.
Even if Beni+21 is suppressed from the absorption wavelength of A,
It is known that blackening progresses.

Therefore, in order to block the light rays over a wide range, it is necessary to form a film that blocks the light rays on the epidermis, or,
Substances that scatter light on the epidermis are used. For example, ultrafine powders such as mineral titanium oxide, talc, and kaolin have been incorporated into creams and the like.

Usually, these powder substances are blended together with the above-mentioned PABA. For example, one such product is a foundation cream (suncake).

Also, some sun protection agents are applied and rubbed onto the skin, making it appear as if you have sunburn (wheat-colored skin), and also causing damage to the pigment itself. Some are designed to block or absorb ultraviolet or visible light.

As a typical example, European cosmetic formulations include products in which PABA and the like are added together with β-carotene or β-carotene-containing carrot oil. However, PABA.

For some people, dry skin can cause contact dermatitis, so an alternative substance is needed.

On the other hand, skin damage and aging occur not only with ultraviolet rays (UV-B) and ultraviolet rays (UV-A), but also with visible light. Therefore, in order to prevent skin damage and aging, it is necessary to absorb both ultraviolet and visible light. A formulation with a wide range of light absorption effects is desired.

(Problems to be Solved by the Invention) The present invention basically solves the problems of ultraviolet light (UV-A), (UV-A),
The aim is to develop new sun protection agents that absorb and block B) on the epidermis and further absorb or block a wide range of wavelengths from ultraviolet to visible light.

In other words, the present invention makes it an essential condition to contain a substance capable of absorbing ultraviolet rays, and furthermore,
We are looking for a substance that absorbs visible light from nm to around 500,600 nm, and the conditions for its development are that it is a natural product other than minerals, and is among the substances derived from animals and plants. In addition, we set the following points as development themes and conducted research on them.

In other words, many powder substances that are insoluble in water and fats and oils absorb or block ultraviolet rays and visible rays, and typical examples include ff1Th such as titanium oxide, talc, and kaolin. There is a system of substances.

However, because it is insoluble in water and oil, it is necessary to form it into a fine powder. Although fine powder improves adhesion to the skin, it conversely causes the fine powder components to enter the sweat glands and sebaceous glands of the epidermis. This will reduce the metabolic function of the skin. In addition, the fine powder components that have entered the face cannot be easily washed off even when the face is washed.

(B) Composition of the Invention The components that constitute the present invention and play the leading role are:
(1) (1) Baicalin, (2) Baicalein, (3)
Senegasaponin, (4) Ondisaponin, any one or more of these components, or any one of the above (11 to (4))
(5) Astaxanthin, (6) β-
M1 of any one of carotene and (7) shikonin.
It is in a skin anointing material that requires a certain combination (composition).

Specifically, the details will be described below.

(Means for solving the problem) [Screening method (1)] In order to solve the above-mentioned drawbacks, the present inventors have developed a method using extracts (ingredients) derived from highly safe animals and plants. First, we searched for usable substances by calculating the 5 minute (0, D) flci from any soluble component such as water, oil, fat, or ethanol.

[Screening method (2)] Next, the inventors investigated whether there is any difference between the screening method (1) described above and the measurement method using a rotary spectrophotometer. , and then rub the substance onto the quartz plate.

The effect of the substance targeted by the present invention was finally determined by determining the transmittance of ultraviolet rays and visible light using a spectrophotometer.

That is, it is premature to conclude that by screening method (1), by showing the ability to absorb visible light from ultraviolet rays, it is determined that this is a drug that can protect the skin 115 from light rays.

In other words, to show this more specifically, 2 (2-hydroxy-
5-methylphenyl)benzotriaz
When measuring using ale: benzotriazole P based on the screening method (1), as shown in No. 1 [2I, ultraviolet absorption ability is observed or the screening method (
When measuring based on 2), as shown in Figure 11,
In fact, even if benzotriazole P is added to the formulation to form a formulation, the ultraviolet protection effect that is the objective of the present invention cannot be obtained.

In other words, when it comes to ultraviolet absorbers that have traditionally been used in cosmetics, screening methods (1) have often been applied. Therefore, it was necessary to add the test J-j.

Therefore, based on the screening method (1), the present inventor further searched for substances that pass both screening methods using the screening method (2) for the marked substances with ultraviolet absorption ability. It hit.

In other words, the substances searched by screening method (2) are
It truly protects the skin from sunlight in the same way as W4-based ingredients such as titanium diluted.

[Substances searched from screening method (1)] t53~
Figure 9 was marked in one test. It is a chart showing each absorbance.

That is, (1) we were able to select baicalin, baicalein, senegasaponin, and ondisaponin. Of course, these are essential components to solve the present invention.

In other words, as is clear from the comparison with PABA in Figure 2, the substances marked in the single test have absorbance up to around 400 nm, indicating that they are ultraviolet absorbers over a wide range of 0 nm.

Alternatively, Senegasaponin and Ondisaponin are called Sunburn. Saponin is said to particularly block light in the wavelength range of 300 to 320 nm.In addition, saponin acts as an ultraviolet absorber and also has a surfactant effect, making it easy to incorporate into creams, emulsions, etc. have. Therefore, it was found that the UV absorber has the advantage of being able to be incorporated into water-soluble bases and oil bases.

[Combination of Substances Searched by Screening Method (2)] Based on the results of the screening method (1), the present inventors have investigated the effect of blocking a wide range of light from the ultraviolet region to the visible region. A lubricant (
Based on the following prescription, we prepared the following standard transparent base agent and mixed it with it to determine its permeation (blocking) effect.

In other words, when preparing a standard transparent base agent, a drug (base agent) that transmits a wide range of X and l1iI from the ultraviolet to the visible region is selected, and the desired target substance is added thereto. We conducted a search to find out what range of light rays can be blocked, and what combinations can be used to make it possible to block light rays over a wide range.

(Standard transparent base agent made by 7A: Cream base formulation A) Polyvinylpyrrolidone 15g Polyethylene glycol 15g Sodium alginate
Add 20g of gum arabic and refined ice aF& to bring the total amount to 500g.

The prescription A is a prescription that transmits visible light from ultraviolet rays well, and is similar to No. 1 O12I.

(Example 1) (1) Baicalin or Hyicalein tgCream base of the above formulation A 90 [Twin 80
log (grade results are 12th to 13th [
) (Example 2) Senegasaponin or Ondisaponin Ig Cream base of the above formulation A 90g purified water
10g (The results are shown in Figures 14-15.
) FIGS. 12 and 13 are charts showing the transmittance of the prescription using (1) baicalin and baicalein according to Example 1.

Figures 14 and 15 are charts showing the transmittance of Senega saponin or Ondisaponin according to Example 2. In other words, both have ha that does not transmit ultraviolet rays up to 350 nm and is superior to PABA as an ultraviolet absorber. I understand that there is something.

However, our objective is to further
Ranging from nm to visible region of 400 nm or more.

One of the important themes in development is the ability to block a wide range of light rays.

Therefore, based on the data obtained, we will conduct further research by docking (combining formulations) of each substance.

Namely, astaxanthin and β-carotene.

Shiconin, Shiconic Liquid, and Shiconic Liquid AB have the ability to absorb light in the visible region.

On the other hand, since senegasaponin or ondisaponin and (1) baicalin and baicalein have the ability to absorb light in the ultraviolet region, a combination of these two Thfis is used. I searched again. As a result, it was found that the particular combinations shown in Examples 3 to 14 were advantageous in blocking light rays over a wide range. Based on these combinations (Example 3 to Example 14), if the effects of some corresponding combinations are confirmed, the 16th to 2712th
It's like I.

(Example 3) (1) Baicalin or Hykalein 1g Astaxanthin or β-carotene Ig Box 1 Cream base of prescription A 90g Twin 80
Log (The results are shown in Figures 16-17)
(Example 4) Senegasaponin or Ondisaponin 1g Astaxanthin or β-carotene Ig Cream base of the above formulation A 50g Twinso 5
0g (The results are shown in Figures 18-19) (Example 5) (1) Baicalin or Hykalein 1g Shikonin 1g 1111 Prescription A
Cream base 50g twin so
50g (The results are shown in Figure 20) (Example 6) (1) Baicalin or Baicalein 1g Ciconic Liquid 30g The above prescription cream base 35g Twin 80
35g (Example 7) Senegasaponin or Ondisaponin 1g Shikonin
1g Cream base of prescription A 50g Twin 80 5
0g (The results are shown in Figure 21) (Example 8) Senegasaponin or Ondisaponin 1g Ciconix Surikint 30g The above prescription cream base 35 to Twin 80
35g (Example 9) (1) Baicalin or Hykalein 1g Senegasaponin or Ondisaponin 1g Astaxanthin or β-carotene 1g Shikonin
1g Cream base of the above prescription A 50g Twin go 50g (Results are shown in Section 22 [2I) (Example 10) (1) Baicalin or Baicalein 1g Shiconin Ig Shiconic Liquid Hachihe 30g Cream base of the above prescription 35g Twin 80 35g
(The results are shown in Figure 23.) (Example 11) Senegasaponin or Ondisaponin 1g Shikonin
1g Sikonic Suri Kit A
8 30g prescribed cream base 3
5g Twin 8Q 35g (The results are shown in Figure 24) (Example 12) (1) Baicalin or Heicalein 1g Astaxanthin or β-carotene 1g Ciconix Liquid A8 30g The above prescription cream base
35g Twin 80 35g (The results are shown in Figure 25) (Example 13) Senegasaponin or Ondisaponin 1g Astaxanthin or β-carotene Ig Siconic Liquid A8
30g prescribed cream base 35
g Twin 80 35g (The results are shown in Figure 26) (Example 14) (1) Baicalin or Hykalein Ig Senegasaponin or Ondisaponin 1g Astaxanthin or β-carotene Tg Shikonin
tg chiconic liquid A8 30g prescription cream base 35g twin 80
35g (Results are No. 27113)
) Next, based on the above-mentioned embodiments, further explanation will be given as follows.

In Examples 3 to 4, (1) either one of baicalin or hyicalein, senegasaponin or ondisaponin is used in combination with astaxanthin or β-carotene, or a more favorable formulation than alone. I understand that there is something. In addition, a combination with shikonin or an extract containing it is also effective.

That is, as shown in Figures 16 to 19, 5
It has become possible to block a wide range of light rays up to the visible range of 0.00 mm.

Looking at the effects from Examples S to 9, the second
As shown in Figures 0 to 22, it was found that a wide range of light rays from the ultraviolet region to the visible region up to 550 m was blocked, and good results were obtained.

Next, the effects corresponding to the combinations of Examples 10 to 14 are as follows:
As shown in FIGS. 23-27.

RI7 has the ability to block a wide range of light from the ultraviolet region to the visible region up to 600 meters, and as a result, it is a wide range of light absorbers that can act as both an ultraviolet absorber and a visible light absorber. As.

Valid M! I can see that it is a combination.

In the examples, in order to concretely demonstrate the effects, specific bases were used, or in actual formulation, they were based on various bases based on the purpose of use, regardless of the actual formulation. It's up to you,
You can use it selectively.

Next, among the drugs (substances) used in Examples etc., Senega or Onji saponin (or extract), astaxanthin, or carrot oil were obtained by the first method.

β-carotene is a reagent manufactured by Kishida Chemical.

Shikonin is manufactured by Mitsui Petrochemical.

(1) Baicalin and baicalein are extracted from the herbal drug Scutellariae, and are manufactured by Maru Falcos.

Shiconix extract (Shiconic Liquid Kit, Shiconic Liquid AB) is manufactured by Maru Falcos.

Scutellariae extract (Scutellaria scutellariae liquid E, Scutellariae liquid B) is manufactured by Maru Falcos.

There are no particular limitations on the manufacturing method or extraction conditions, and it is sufficient to use extracts, crude extracts, or solutions thereof containing the respective main components.

(Senega saponin and extract extraction method 1) 500 g of Senega spp.
Extract with heating, separate the methanol layer, concentrate under reduced pressure,
Approximately 200 g of crude extract is obtained. Dissolve this extract in water,
Extract with n-butanol, separate the n-butanol layer, and concentrate under reduced pressure to obtain about 100 g of purified extract. This purified extract is washed with ether to obtain about 50 g of Senega saponin as a white powder.

(Senega saponin and extract extraction method 2) After washing 500 g of Senega saponin or powder collected daily with ether, 10 M of methanol is added and extracted by heating. The methanol layer was separated, concentrated under reduced pressure, and ether 1 to 1 was added to the cliI solution.
Add twice the amount. The precipitate is obtained as an extract. Methanol 141 is added to this extract to dissolve it, and two portions of cholesterol-saturated 95% ethanol aqueous solution (or 95% ethanol aqueous solution) are added to obtain a precipitate. After washing well with ether, white powder, approximately 30g of Senega saponin
is obtained.

(Method for Extracting Saponin and Extract) After washing 500 g of Saponin listed in the diurnal list with ether, add 10 g of methanol, heat extraction, and separate the methanol layer. Concentrate under reduced pressure to obtain a concentrated solution of the crude extract. 1. Next, add twice the amount of ether to the concentrated solution, shake well, and evaporate to separate the precipitated extract. By adding 15 g of methanol to this extract and dissolving it, and then adding 3 g of 95% ethanol aqueous solution and leaving it to stand, the precipitate was dissolved.
After being drained, it is collected and washed with ether to obtain about 6 g of white powder of Ondisaponin.

<Astaxanthin and Shrimp Extraction Pigment Extraction Method> Krill When dried milled crab (crayfish) and shrimp (both boiled and then dried and crushed) are extracted using a Soxhlet extractor using ether, all of the krill is extracted. Approximately 18% is obtained for lipids, and 4 to 5% for crabs and shrimps. In this total lipid, there are 10
Approximately 100 to 200 mg of astaxanthin is contained in 0 g of lipid. Also, for crabs and shrimp, lOO~300
Contains after m g i'iii.

It is called pigment oil and is usually extracted with acetone, ethyl acetate, hexane, etc., and is contained in this liquid crude oil.

To 100 g of the obtained dye extraction was added one methanol to dissolve it, a 10% LA solution of sodium hydroxide in methanol was added, and the mixture was stirred at 65° C. for 230 minutes. Two portions of purified water were added, extracted with three portions of hexane, and the hexane layer was concentrated under reduced pressure to obtain a purified oil. This purified oil can be passed through an alumina column to obtain astaxanthin, which is eluted with benzene.

(Carronto oil extraction for 1 person) Extract by adding n-hexane to edible carrots.

Obtained by fk reduction of the n-hexane layer.

[C] Effects of the Invention The present invention provides a drug that exhibits absorption ability (rate) over a wide range from ultraviolet rays or ultraviolet rays to visible light.

Based on the specific substances marked in these re-tests, the blocking ability was determined, and by incorporating them into cosmetic bases or ointment bases, it was possible to apply them to the skin to prevent sunburn. It consists in applying a lubricant (external preparation).

Each of the specified substances used in the present invention is readily known (existing substances).

However, until now, the field of application has not been sufficient.

On the other hand, the present invention utilizes ultraviolet rays or UV rays based on the physical properties of existing animal and plant extracts.

Based on absorption and transmission abilities ranging from ultraviolet rays to visible light,
We were able to develop a field of application for a practical skin-applying paint.

That is, 1. The fact that external preparations or cosmetics that protect the skin from direct sunlight have been created by using raw sand or combination conditions of the specified substances according to the present invention has great cosmetic effects. There is.

That is, from a cosmetic point of view, prolonged exposure to direct sunlight causes aging of skin tissue cells, a decrease in elastic m fibers (elastin), an increase in collagen fibers, and a concomitant decrease in elastin. The present invention is effective as a protective agent for these factors, such as an increase in wrinkles, a decrease in the moisturizing ability of the keratin layer of the skin, and the occurrence of rough skin due to dryness.

In other words, the effect shown in the case of +111 can be expected to be effective against not only the acute symptom of 1 sunburn but also the chronic symptom (foaming).

[Brief Description of the Drawings] Figures 1 to 9 are ultraviolet absorption spectra measured by a 5 spectrophotometer (cell) for ultraviolet rays of each substance according to screening method (1). FIG. 1 shows an ethanol solution containing 1% pencitreazole P. Figure 2 shows an ethanol solution containing 0.001% para-aminobenzoic acid. FIG. 3 shows (1) 0.001% baicalin-containing ethanol solution. FIG. 4 shows an extract containing an ethanol solution containing 1% Picalan 01001 (Ougori Liquid B). No. 5I12I is an extract containing an ethanol solution containing 205001% Pikari (Ougori Kit E). FIG. 6 shows an ethanol solution containing 0.001% hikalein. FIG. 7 shows an extract (hyicalein extract) containing an ethanol solution containing 0 and % hyicalein O, and FIG. 8 shows an aqueous solution containing 1% Senegasaponin 01O. No. 9 UA is an aqueous solution containing 0.01% ondisaponin. The 10th to 26th UA are 1 spectrophotometer (
Transmittance by quartz plate). Figure 1O is cream-based formulation A. FIG. 11 shows an olive oil solution containing 1% pencitreazole P. Figure 12 shows (1) Baicalin. The t513 diagram is Hykarein. 14th [2 is Senega saponin. Figure 15 shows ondisaponin. Figure 16 shows (1) Baicalin and astaxanthin. Figure 17 shows baicalein and β-carotene. Figure 18 shows Senega saponin and astaxanthin. :tSlQ diagram shows Senega saponin and β-carotene. Figure 20 shows hycalin and shikonin. Figure 21 shows Senegasaponin and Shikonin. Figure 22 shows picalein, senegasaponin, astaxanthin, and shikonin. FIG. 23 shows (1) Baicalin, Shikonin, and Shiconic Liquid Kit AB. No. 2412 is Senegasaponin, Shikonin, and Shikonisoxurikit AB. m 25 [21 is baicalein, β-carotene, and ciconix liquid kit AB. Figure 26 shows Senega saponin, astaxanthin, and Ciconicus sochiuta AB. FIG. 27 shows hyicalein, Senegasaponin, β-carotene, Shikonin, and Shiconix Liquid Kit AB.

Claims (1)

[Scope of Claims] (1) Contains one or more of the following: (1) Baicalin, (2) Baicalein, (3) Senegasaponin, (4) Ondisaponin, or (1) to (
A skin-application paint characterized by containing any one or more of (5) astaxanthin, (6) β-carotene, and (7) shikonin together with any one or more of 4).