JPS637780A - 細胞への鉄供給方法 - Google Patents
細胞への鉄供給方法Info
- Publication number
- JPS637780A JPS637780A JP61152111A JP15211186A JPS637780A JP S637780 A JPS637780 A JP S637780A JP 61152111 A JP61152111 A JP 61152111A JP 15211186 A JP15211186 A JP 15211186A JP S637780 A JPS637780 A JP S637780A
- Authority
- JP
- Japan
- Prior art keywords
- iron
- serum
- cells
- chelating agent
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 129
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims description 21
- 239000001963 growth medium Substances 0.000 title abstract description 8
- 239000002738 chelating agent Substances 0.000 claims abstract description 45
- 102000004338 Transferrin Human genes 0.000 claims abstract description 40
- 108090000901 Transferrin Proteins 0.000 claims abstract description 40
- 239000012581 transferrin Substances 0.000 claims abstract description 39
- -1 iron ions Chemical class 0.000 claims abstract description 33
- 239000013522 chelant Substances 0.000 claims abstract description 6
- NBZBKCUXIYYUSX-UHFFFAOYSA-N iminodiacetic acid Chemical compound OC(=O)CNCC(O)=O NBZBKCUXIYYUSX-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000007935 neutral effect Effects 0.000 claims abstract description 6
- JYXGIOKAKDAARW-UHFFFAOYSA-N N-(2-hydroxyethyl)iminodiacetic acid Chemical compound OCCN(CC(O)=O)CC(O)=O JYXGIOKAKDAARW-UHFFFAOYSA-N 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 76
- 239000002609 medium Substances 0.000 claims description 59
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 7
- 239000007640 basal medium Substances 0.000 claims description 6
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 5
- 210000004102 animal cell Anatomy 0.000 claims description 5
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 2
- RMHBCYUXZMMSFP-UHFFFAOYSA-N Cl.NCCN.CCC(O)=O.CCC(O)=O Chemical compound Cl.NCCN.CCC(O)=O.CCC(O)=O RMHBCYUXZMMSFP-UHFFFAOYSA-N 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 35
- 210000002966 serum Anatomy 0.000 description 24
- 238000012360 testing method Methods 0.000 description 16
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 12
- 239000000306 component Substances 0.000 description 11
- 230000004663 cell proliferation Effects 0.000 description 10
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 10
- 108010088751 Albumins Proteins 0.000 description 9
- 102000009027 Albumins Human genes 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 7
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- 102000004877 Insulin Human genes 0.000 description 6
- 108090001061 Insulin Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000001569 carbon dioxide Substances 0.000 description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 description 6
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- 229940125396 insulin Drugs 0.000 description 6
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- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- 210000004748 cultured cell Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 235000004252 protein component Nutrition 0.000 description 4
- 229910052711 selenium Inorganic materials 0.000 description 4
- 239000011669 selenium Substances 0.000 description 4
- 229940091258 selenium supplement Drugs 0.000 description 4
- 239000004017 serum-free culture medium Substances 0.000 description 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 4
- 229960005486 vaccine Drugs 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
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- 239000011790 ferrous sulphate Substances 0.000 description 3
- 235000003891 ferrous sulphate Nutrition 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 108010045676 holotransferrin Proteins 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 150000002505 iron Chemical class 0.000 description 3
- 150000002506 iron compounds Chemical class 0.000 description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 3
- 210000003292 kidney cell Anatomy 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- XNSAINXGIQZQOO-SRVKXCTJSA-N protirelin Chemical compound NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H]1NC(=O)CC1)CC1=CN=CN1 XNSAINXGIQZQOO-SRVKXCTJSA-N 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229940035722 triiodothyronine Drugs 0.000 description 3
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- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
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- 239000011780 sodium chloride Substances 0.000 description 1
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61152111A JPS637780A (ja) | 1986-06-28 | 1986-06-28 | 細胞への鉄供給方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61152111A JPS637780A (ja) | 1986-06-28 | 1986-06-28 | 細胞への鉄供給方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS637780A true JPS637780A (ja) | 1988-01-13 |
| JPH0568231B2 JPH0568231B2 (zh) | 1993-09-28 |
Family
ID=15533298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61152111A Granted JPS637780A (ja) | 1986-06-28 | 1986-06-28 | 細胞への鉄供給方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS637780A (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63141584A (ja) * | 1986-12-04 | 1988-06-14 | Chemo Sero Therapeut Res Inst | 細胞培養用培地 |
| USRE39792E1 (en) | 1990-10-17 | 2007-08-21 | Smithkline Beecham Corporation | Method for culturing Chinese hamster ovary cells |
-
1986
- 1986-06-28 JP JP61152111A patent/JPS637780A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63141584A (ja) * | 1986-12-04 | 1988-06-14 | Chemo Sero Therapeut Res Inst | 細胞培養用培地 |
| JPH0510074B2 (zh) * | 1986-12-04 | 1993-02-08 | Kagaku Oyobi Ketsusei Ryoho Kenkyusho | |
| USRE39792E1 (en) | 1990-10-17 | 2007-08-21 | Smithkline Beecham Corporation | Method for culturing Chinese hamster ovary cells |
| USRE41974E1 (en) | 1990-10-17 | 2010-11-30 | Glaxosmithkline Llc | Method for culturing Chinese hamster ovary cells |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0568231B2 (zh) | 1993-09-28 |
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| LAPS | Cancellation because of no payment of annual fees |