JPS6350665B2 - - Google Patents
Info
- Publication number
- JPS6350665B2 JPS6350665B2 JP13847681A JP13847681A JPS6350665B2 JP S6350665 B2 JPS6350665 B2 JP S6350665B2 JP 13847681 A JP13847681 A JP 13847681A JP 13847681 A JP13847681 A JP 13847681A JP S6350665 B2 JPS6350665 B2 JP S6350665B2
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- free cholesterol
- analysis
- sodium
- container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 68
- 238000006243 chemical reaction Methods 0.000 claims description 50
- 235000012000 cholesterol Nutrition 0.000 claims description 34
- 238000004458 analytical method Methods 0.000 claims description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims description 19
- 229920003002 synthetic resin Polymers 0.000 claims description 14
- 239000000057 synthetic resin Substances 0.000 claims description 14
- 108010013563 Lipoprotein Lipase Proteins 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 7
- BCKXLBQYZLBQEK-KVVVOXFISA-M Sodium oleate Chemical compound [Na+].CCCCCCCC\C=C/CCCCCCCC([O-])=O BCKXLBQYZLBQEK-KVVVOXFISA-M 0.000 claims description 5
- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- BTURAGWYSMTVOW-UHFFFAOYSA-M sodium dodecanoate Chemical compound [Na+].CCCCCCCCCCCC([O-])=O BTURAGWYSMTVOW-UHFFFAOYSA-M 0.000 claims description 3
- 229940082004 sodium laurate Drugs 0.000 claims description 3
- 229940045870 sodium palmitate Drugs 0.000 claims description 3
- 229940080350 sodium stearate Drugs 0.000 claims description 3
- GGXKEBACDBNFAF-UHFFFAOYSA-M sodium;hexadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCC([O-])=O GGXKEBACDBNFAF-UHFFFAOYSA-M 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 102100022119 Lipoprotein lipase Human genes 0.000 claims 1
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 102000043296 Lipoprotein lipases Human genes 0.000 description 9
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 8
- 238000005259 measurement Methods 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 6
- -1 alkali metal salts Chemical class 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 6
- 229930195729 fatty acid Natural products 0.000 description 6
- 239000000194 fatty acid Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 229910052783 alkali metal Inorganic materials 0.000 description 4
- 108010089254 Cholesterol oxidase Proteins 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 102000004895 Lipoproteins Human genes 0.000 description 2
- 108090001030 Lipoproteins Proteins 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 239000000113 methacrylic resin Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229920005990 polystyrene resin Polymers 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- NYOXRYYXRWJDKP-GYKMGIIDSA-N cholest-4-en-3-one Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 NYOXRYYXRWJDKP-GYKMGIIDSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 108010090622 glycerol oxidase Proteins 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13847681A JPS5841357A (ja) | 1981-09-04 | 1981-09-04 | 遊離コレステロ−ル分析法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13847681A JPS5841357A (ja) | 1981-09-04 | 1981-09-04 | 遊離コレステロ−ル分析法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5841357A JPS5841357A (ja) | 1983-03-10 |
| JPS6350665B2 true JPS6350665B2 (enrdf_load_stackoverflow) | 1988-10-11 |
Family
ID=15222953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13847681A Granted JPS5841357A (ja) | 1981-09-04 | 1981-09-04 | 遊離コレステロ−ル分析法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5841357A (enrdf_load_stackoverflow) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0372093A (ja) * | 1989-08-09 | 1991-03-27 | Mitsubishi Motors Corp | 亜鉛含有メッキ酸性溶液 |
| BR112014025875B1 (pt) * | 2012-04-27 | 2021-09-28 | Hitachi Chemical Diagnostics Systems Co., Ltd | Método de medição |
-
1981
- 1981-09-04 JP JP13847681A patent/JPS5841357A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5841357A (ja) | 1983-03-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lundquist | The determination of ethyl alcohol in blood and tissues | |
| EP0041366B1 (en) | Method for operating an apparatus for analysing samples optically | |
| US20210033503A1 (en) | Apparatus for automated analysis | |
| JP7361492B2 (ja) | 試験試料に含有される干渉物質を分離するための検査室システムおよび方法 | |
| JP2007524081A (ja) | モジュール式試薬給送手段を使用することによって自動臨床アナライザの性能を向上させる方法 | |
| AU5925086A (en) | Unitized reagent containment system for clinical analyzer | |
| EP3149475B1 (en) | Method and apparatus for reducing carryover of reagents and samples in analytical testing | |
| JPS6350665B2 (enrdf_load_stackoverflow) | ||
| CA2138536C (en) | Chemiluminescent assay for dsdna antibodies | |
| JPH07159416A (ja) | 自動化学分析装置 | |
| EP0231191A1 (en) | Measurement of total iron binding capacity | |
| Cerón et al. | Automated spectrophotometric method using 2, 2’-dithiodipyridine acid for determination of cholinesterase in whole blood | |
| EP0806671B1 (en) | Automated urinalysis system for detecting blood in urine | |
| US3723063A (en) | Process for determination of chemical constituents of proteinaceous biological fluids | |
| JPS6345055B2 (enrdf_load_stackoverflow) | ||
| JPS627840B2 (enrdf_load_stackoverflow) | ||
| Achilles et al. | Microdetermination of urinary constituents by vertical light-path photometry in microplates | |
| CN107064534A (zh) | 用于操作自动分析机器的方法 | |
| JPS6243490B2 (enrdf_load_stackoverflow) | ||
| JPH0217077B2 (enrdf_load_stackoverflow) | ||
| ZIEGENHORN et al. | Kinetic Enzymatic Method for | |
| JPH03146867A (ja) | 自動分析装置 | |
| JPS5867197A (ja) | 遊離型コレステロ−ル測定用試薬 | |
| JPS61226661A (ja) | 自動分析装置 | |
| DE3617707A1 (de) | Verfahren zur durchfuehrung immunologischer bestimmungen |