JPS6349086A - ストレプトミセテスプラスミドでの色素マ−カ− - Google Patents
ストレプトミセテスプラスミドでの色素マ−カ−Info
- Publication number
- JPS6349086A JPS6349086A JP62202585A JP20258587A JPS6349086A JP S6349086 A JPS6349086 A JP S6349086A JP 62202585 A JP62202585 A JP 62202585A JP 20258587 A JP20258587 A JP 20258587A JP S6349086 A JPS6349086 A JP S6349086A
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- streptomycetes
- marker
- gene
- fragment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000013612 plasmid Substances 0.000 title claims description 20
- 239000003550 marker Substances 0.000 title claims description 14
- 241001655322 Streptomycetales Species 0.000 title claims description 5
- 239000000126 substance Substances 0.000 claims description 16
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 239000012634 fragment Substances 0.000 claims description 14
- 241000187747 Streptomyces Species 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000010367 cloning Methods 0.000 claims description 4
- 230000002779 inactivation Effects 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 4
- 101100269389 Mus musculus Pgm3 gene Proteins 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 229930193140 Neomycin Natural products 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 229960004927 neomycin Drugs 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 101100122010 Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50) glmM gene Proteins 0.000 description 1
- 101100001347 Mus musculus Akt1s1 gene Proteins 0.000 description 1
- 241000187398 Streptomyces lividans Species 0.000 description 1
- NSFFHOGKXHRQEW-UHFFFAOYSA-N Thiostrepton B Natural products N1C(=O)C(C)NC(=O)C(=C)NC(=O)C(C)NC(=O)C(C(C)CC)NC(C(C2=N3)O)C=CC2=C(C(C)O)C=C3C(=O)OC(C)C(C=2SC=C(N=2)C2N=3)NC(=O)C(N=4)=CSC=4C(C(C)(O)C(C)O)NC(=O)C(N=4)CSC=4C(=CC)NC(=O)C(C(C)O)NC(=O)C(N=4)=CSC=4C21CCC=3C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-UHFFFAOYSA-N 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 101150114087 aphI gene Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000007362 sporulation medium Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 229930188070 thiostrepton Natural products 0.000 description 1
- NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 1
- 229940063214 thiostrepton Drugs 0.000 description 1
- NSFFHOGKXHRQEW-OFMUQYBVSA-N thiostrepton A Natural products CC[C@H](C)[C@@H]1N[C@@H]2C=Cc3c(cc(nc3[C@H]2O)C(=O)O[C@H](C)[C@@H]4NC(=O)c5csc(n5)[C@@H](NC(=O)[C@H]6CSC(=N6)C(=CC)NC(=O)[C@@H](NC(=O)c7csc(n7)[C@]8(CCC(=N[C@@H]8c9csc4n9)c%10nc(cs%10)C(=O)NC(=C)C(=O)NC(=C)C(=O)N)NC(=O)[C@H](C)NC(=O)C(=C)NC(=O)[C@H](C)NC1=O)[C@@H](C)O)[C@](C)(O)[C@@H](C)O)[C@H](C)O NSFFHOGKXHRQEW-OFMUQYBVSA-N 0.000 description 1
- 239000001974 tryptic soy broth Substances 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Road Signs Or Road Markings (AREA)
- Vessels And Coating Films For Discharge Lamps (AREA)
- Ignition Installations For Internal Combustion Engines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3627405.4 | 1986-08-13 | ||
| DE19863627405 DE3627405A1 (de) | 1986-08-13 | 1986-08-13 | Farbmarker in streptomyceten-plasmiden |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6349086A true JPS6349086A (ja) | 1988-03-01 |
Family
ID=6307275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62202585A Pending JPS6349086A (ja) | 1986-08-13 | 1987-08-13 | ストレプトミセテスプラスミドでの色素マ−カ− |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US4963496A (enExample) |
| EP (1) | EP0257417B1 (enExample) |
| JP (1) | JPS6349086A (enExample) |
| AT (1) | ATE76101T1 (enExample) |
| AU (1) | AU595917B2 (enExample) |
| CA (1) | CA1288070C (enExample) |
| DE (2) | DE3627405A1 (enExample) |
| DK (1) | DK420787A (enExample) |
| ES (1) | ES2032411T3 (enExample) |
| FI (1) | FI873482A7 (enExample) |
| GR (1) | GR3005327T3 (enExample) |
| HU (1) | HU204304B (enExample) |
| IL (1) | IL83508A (enExample) |
| NO (1) | NO873386L (enExample) |
| PT (1) | PT85526B (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2328209A (en) * | 1997-08-16 | 1999-02-17 | Stefan Andreas Oehler | Vectors which express a coloured protein for detection of integration into a cell |
| CN1321119C (zh) * | 2003-01-17 | 2007-06-13 | 华东理工大学 | λ－放线紫素及其制备方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4666846A (en) * | 1984-02-17 | 1987-05-19 | Eli Lilly And Company | Novel cloning vectors containing selectable genetic markers for use in streptomyces and related organisms |
| DE3440735A1 (de) * | 1984-11-08 | 1986-05-15 | Hoechst Ag, 6230 Frankfurt | Bakterienlysierendes enzymprodukt aus streptomyceten, verfahren zu seiner herstellung und dafuer geeigneter stamm |
-
1986
- 1986-08-13 DE DE19863627405 patent/DE3627405A1/de not_active Withdrawn
-
1987
- 1987-08-08 DE DE8787111509T patent/DE3779012D1/de not_active Expired - Fee Related
- 1987-08-08 EP EP87111509A patent/EP0257417B1/de not_active Expired - Lifetime
- 1987-08-08 AT AT87111509T patent/ATE76101T1/de not_active IP Right Cessation
- 1987-08-08 ES ES198787111509T patent/ES2032411T3/es not_active Expired - Lifetime
- 1987-08-11 US US07/083,806 patent/US4963496A/en not_active Expired - Fee Related
- 1987-08-11 FI FI873482A patent/FI873482A7/fi not_active Application Discontinuation
- 1987-08-12 IL IL83508A patent/IL83508A/xx not_active IP Right Cessation
- 1987-08-12 AU AU76802/87A patent/AU595917B2/en not_active Ceased
- 1987-08-12 CA CA000544346A patent/CA1288070C/en not_active Expired - Fee Related
- 1987-08-12 DK DK420787A patent/DK420787A/da not_active Application Discontinuation
- 1987-08-12 PT PT85526A patent/PT85526B/pt not_active IP Right Cessation
- 1987-08-12 HU HU873646A patent/HU204304B/hu unknown
- 1987-08-12 NO NO873386A patent/NO873386L/no unknown
- 1987-08-13 JP JP62202585A patent/JPS6349086A/ja active Pending
-
1992
- 1992-07-31 GR GR920401370T patent/GR3005327T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE76101T1 (de) | 1992-05-15 |
| FI873482L (fi) | 1988-02-14 |
| IL83508A (en) | 1991-08-16 |
| HU204304B (en) | 1991-12-30 |
| DK420787A (da) | 1988-02-14 |
| HUT44617A (en) | 1988-03-28 |
| PT85526B (pt) | 1990-06-29 |
| EP0257417A3 (en) | 1988-11-09 |
| EP0257417B1 (de) | 1992-05-13 |
| EP0257417A2 (de) | 1988-03-02 |
| AU595917B2 (en) | 1990-04-12 |
| AU7680287A (en) | 1988-02-18 |
| IL83508A0 (en) | 1988-01-31 |
| CA1288070C (en) | 1991-08-27 |
| ES2032411T3 (es) | 1993-02-16 |
| US4963496A (en) | 1990-10-16 |
| DE3627405A1 (de) | 1988-02-18 |
| NO873386D0 (no) | 1987-08-12 |
| DE3779012D1 (de) | 1992-06-17 |
| PT85526A (de) | 1987-09-01 |
| DK420787D0 (da) | 1987-08-12 |
| GR3005327T3 (enExample) | 1993-05-24 |
| FI873482A0 (fi) | 1987-08-11 |
| FI873482A7 (fi) | 1988-02-14 |
| NO873386L (no) | 1988-02-15 |
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