JPS6330286B2 - - Google Patents

Info

Publication number

JPS6330286B2

JPS6330286B2 JP57011116A JP1111682A JPS6330286B2 JP S6330286 B2 JPS6330286 B2 JP S6330286B2 JP 57011116 A JP57011116 A JP 57011116A JP 1111682 A JP1111682 A JP 1111682A JP S6330286 B2 JPS6330286 B2 JP S6330286B2

Authority

JP

Japan

Prior art keywords

substance

mice

chlorella

administered

glycoprotein

Prior art date

1982-01-27

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired

Links

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• 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 12
• 102000003886 Glycoproteins Human genes 0.000 claims description 7
• 108090000288 Glycoproteins Proteins 0.000 claims description 7
• 239000002246 antineoplastic agent Substances 0.000 claims description 6
• 102000004169 proteins and genes Human genes 0.000 claims description 4
• 108090000623 proteins and genes Proteins 0.000 claims description 4
• 235000016425 Arthrospira platensis Nutrition 0.000 claims description 3
• 240000002900 Arthrospira platensis Species 0.000 claims description 3
• 229940082787 spirulina Drugs 0.000 claims description 3
• 241000195663 Scenedesmus Species 0.000 claims description 2
• 229940041181 antineoplastic drug Drugs 0.000 claims description 2
• 239000000126 substance Substances 0.000 description 18
• 241000699670 Mus sp. Species 0.000 description 15
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
• 239000000843 powder Substances 0.000 description 7
• 150000001408 amides Chemical class 0.000 description 6
• 210000004027 cell Anatomy 0.000 description 6
• 238000000034 method Methods 0.000 description 6
• 238000012360 testing method Methods 0.000 description 6
• 241000195493 Cryptophyta Species 0.000 description 5
• XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
• BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 4
• XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 4
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
• 230000009102 absorption Effects 0.000 description 4
• 238000010521 absorption reaction Methods 0.000 description 4
• 125000000539 amino acid group Chemical group 0.000 description 4
• 150000001413 amino acids Chemical class 0.000 description 4
• 239000012528 membrane Substances 0.000 description 4
• 241000894007 species Species 0.000 description 4
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
• 208000028018 Lymphocytic leukaemia Diseases 0.000 description 3
• 241000699666 Mus <mouse, genus> Species 0.000 description 3
• 241000283973 Oryctolagus cuniculus Species 0.000 description 3
• 230000001093 anti-cancer Effects 0.000 description 3
• 239000000872 buffer Substances 0.000 description 3
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• 238000007796 conventional method Methods 0.000 description 3
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• 208000003747 lymphoid leukemia Diseases 0.000 description 3
• 230000004083 survival effect Effects 0.000 description 3
• 229920001817 Agar Polymers 0.000 description 2
• CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
• YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 2
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
• 238000001069 Raman spectroscopy Methods 0.000 description 2
• 208000006268 Sarcoma 180 Diseases 0.000 description 2
• QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
• 239000008272 agar Substances 0.000 description 2
• 230000000259 anti-tumor effect Effects 0.000 description 2
• 229910052786 argon Inorganic materials 0.000 description 2
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• OIPPWFOQEKKFEE-UHFFFAOYSA-N orcinol Chemical compound CC1=CC(O)=CC(O)=C1 OIPPWFOQEKKFEE-UHFFFAOYSA-N 0.000 description 2
• 150000003214 pyranose derivatives Chemical group 0.000 description 2
• 230000000241 respiratory effect Effects 0.000 description 2
• 238000004062 sedimentation Methods 0.000 description 2
• 239000011780 sodium chloride Substances 0.000 description 2
• 239000000243 solution Substances 0.000 description 2
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
• 238000004114 suspension culture Methods 0.000 description 2
• 238000002054 transplantation Methods 0.000 description 2
• 210000004881 tumor cell Anatomy 0.000 description 2
• 210000003462 vein Anatomy 0.000 description 2
• FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical group O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
• 229920000310 Alpha glucan Polymers 0.000 description 1
• 229920002498 Beta-glucan Polymers 0.000 description 1
• GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
• 229920001503 Glucan Polymers 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 238000004566 IR spectroscopy Methods 0.000 description 1
• 229930182555 Penicillin Natural products 0.000 description 1
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
• 239000012980 RPMI-1640 medium Substances 0.000 description 1
• 229920005654 Sephadex Polymers 0.000 description 1
• 239000012507 Sephadex™ Substances 0.000 description 1
• 239000002253 acid Substances 0.000 description 1
• 150000007513 acids Chemical class 0.000 description 1
• 239000000654 additive Substances 0.000 description 1
• 230000000996 additive effect Effects 0.000 description 1
• 239000003513 alkali Substances 0.000 description 1
• 238000004458 analytical method Methods 0.000 description 1
• 239000007864 aqueous solution Substances 0.000 description 1
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
• 239000007853 buffer solution Substances 0.000 description 1
• 239000001569 carbon dioxide Substances 0.000 description 1
• 229910002092 carbon dioxide Inorganic materials 0.000 description 1
• 230000022534 cell killing Effects 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 238000005119 centrifugation Methods 0.000 description 1
• 229940044683 chemotherapy drug Drugs 0.000 description 1
• 238000001142 circular dichroism spectrum Methods 0.000 description 1
• 238000010367 cloning Methods 0.000 description 1
• 230000015271 coagulation Effects 0.000 description 1
• 238000005345 coagulation Methods 0.000 description 1
• 238000004440 column chromatography Methods 0.000 description 1
• 238000012136 culture method Methods 0.000 description 1
• 238000000502 dialysis Methods 0.000 description 1
• 230000008034 disappearance Effects 0.000 description 1
• 239000006185 dispersion Substances 0.000 description 1
• 239000012153 distilled water Substances 0.000 description 1
• 238000001962 electrophoresis Methods 0.000 description 1
• 238000010828 elution Methods 0.000 description 1
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• 238000005194 fractionation Methods 0.000 description 1
• 238000004108 freeze drying Methods 0.000 description 1
• 238000002523 gelfiltration Methods 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 230000009036 growth inhibition Effects 0.000 description 1
• 230000002949 hemolytic effect Effects 0.000 description 1
• 230000001900 immune effect Effects 0.000 description 1
• 238000000760 immunoelectrophoresis Methods 0.000 description 1
• 230000003308 immunostimulating effect Effects 0.000 description 1
• 238000002513 implantation Methods 0.000 description 1
• 230000002401 inhibitory effect Effects 0.000 description 1
• 230000001404 mediated effect Effects 0.000 description 1
• 239000000203 mixture Substances 0.000 description 1
• 230000003287 optical effect Effects 0.000 description 1
• 238000004816 paper chromatography Methods 0.000 description 1
• 229940049954 penicillin Drugs 0.000 description 1
• 239000008363 phosphate buffer Substances 0.000 description 1
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• 230000035755 proliferation Effects 0.000 description 1
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• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
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• 238000004611 spectroscopical analysis Methods 0.000 description 1
• 238000001694 spray drying Methods 0.000 description 1
• 238000010561 standard procedure Methods 0.000 description 1
• 229960005322 streptomycin Drugs 0.000 description 1
• 239000006228 supernatant Substances 0.000 description 1
• 230000001988 toxicity Effects 0.000 description 1
• 231100000419 toxicity Toxicity 0.000 description 1
• 238000003809 water extraction Methods 0.000 description 1