JPS63240782A - Production of tissue like saffron stigma - Google Patents
Production of tissue like saffron stigmaInfo
- Publication number
- JPS63240782A JPS63240782A JP62074949A JP7494987A JPS63240782A JP S63240782 A JPS63240782 A JP S63240782A JP 62074949 A JP62074949 A JP 62074949A JP 7494987 A JP7494987 A JP 7494987A JP S63240782 A JPS63240782 A JP S63240782A
- Authority
- JP
- Japan
- Prior art keywords
- saffron
- tissue
- stigma
- medium
- pigment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000124209 Crocus sativus Species 0.000 title claims abstract description 81
- 235000015655 Crocus sativus Nutrition 0.000 title claims abstract description 81
- 239000004248 saffron Substances 0.000 title claims abstract description 81
- 235000013974 saffron Nutrition 0.000 title claims abstract description 81
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 239000000049 pigment Substances 0.000 claims abstract description 36
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims abstract description 18
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 210000001672 ovary Anatomy 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims description 11
- 239000004615 ingredient Substances 0.000 claims description 9
- 238000000638 solvent extraction Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract description 2
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 abstract 3
- 239000002609 medium Substances 0.000 description 26
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 238000000034 method Methods 0.000 description 6
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical class C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 238000012136 culture method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- -1 Further Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 240000002769 Morchella esculenta Species 0.000 description 1
- 235000002779 Morchella esculenta Nutrition 0.000 description 1
- CZMFZOHCAVLXBW-UHFFFAOYSA-N Pigment S1 Natural products CCC1(O)CC(OC2CC(C(OC3CC(O)C(O)C(C)O3)C(C)O2)N(C)C)c4cc5C(=O)c6c(O)cccc6C(=O)c5cc4C1C(=O)OC CZMFZOHCAVLXBW-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000000596 hypostatic effect Effects 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229940125723 sedative agent Drugs 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、サフランのめしべを構成する花柱と子房とを
、特定の物質を含有する培地で組織培養してサフラン柱
頭様組織を産生させる方法、及び、このようにして産生
させて得たサフラン柱頭様組織から、色素等を採取して
サフラン任用成分を製造する方法に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention produces saffron stigma-like tissue by tissue culturing the style and ovary that constitute the pistil of saffron in a medium containing a specific substance. The present invention relates to a method and a method for producing a saffron ingredient by collecting pigments and the like from the saffron stigma-like tissue produced in this manner.
ここに得られるサフラン「用成分は、医薬（鎮静剤など
）、着色料（色素）、香料、苦味料等として、医薬品、
食品、化粧品等の分野で使用される。The saffron ingredients obtained here are used as pharmaceuticals (sedatives, etc.), colorants (pigments), flavors, bittering agents, etc.
Used in fields such as food and cosmetics.
サフランのめしべは、上部より柱頭、花柱、子房の各部
から構成されているが、この柱頭は、ａ紅色！呈し、サ
フラン色素を含むほか薬用成分、芳香成分などの任用成
分を含んでいる。The saffron pistil consists of the stigma, style, and ovary from the top, and the stigma is a red! In addition to saffron pigment, it also contains medicinal ingredients, aromatic ingredients, and other medicinal ingredients.
従来、このような任用成分を含むサフラン柱頭は、サフ
ランを栽培して花を咲かせ、そのめしべから採取されて
きた。Conventionally, saffron stigmas containing such appointed components have been collected from the pistils of saffron cultivated and blooming flowers.
通常、３０ｇ程度の大きなサフランの球根を栽培しても
、花は６個程度しか着かないので、３個に分裂しためし
べ柱頭は１８本程度しか採れない。Normally, even if you grow a large saffron bulb weighing about 30g, it will only produce about 6 flowers, which means that it will only divide into 3 pieces and produce only about 18 pistil stigmas.
仮りに１ ｉｔのめしべ柱頭を採ろうとすると、必要な
サフランの球＠］は、約５００ ｋｇにもなる。従って
、サフランのめしべ柱頭の生産では、広大な栽培面積が
必要となる。しかも、天然の栽培生産では、時間がかか
り、天候にも大きく影響を受け、かつサフランは、極端
に連作をきらう植物であるので、めしべ柱頭を効率的に
栽培生産することがむずかしく、こうした点からもサフ
ランのめしべ柱頭は、非常に高価な存在となっている。If you were to collect 1 it of pistil stigmas, you would need about 500 kg of saffron balls. Therefore, the production of saffron pistil stigmas requires a large cultivation area. Moreover, natural cultivation takes time and is greatly affected by the weather, and saffron is a plant that extremely dislikes repeated cultivation, so it is difficult to cultivate and produce pistil stigmas efficiently. Even saffron pistil stigmas are very expensive.
最近、一般に天然色素の生産を目的として、植物の組織
培養による色素生産の研究が広く行われているが、今の
ところサフランに関しては十分膚足のいく生産技術はま
だ確立されておらず、サフラン任用成分を得る効率的な
生産技術の開発が要望されている。Recently, research on pigment production using plant tissue culture has been widely conducted with the aim of producing natural pigments. There is a need for the development of efficient production techniques to obtain the desired ingredients.
本発明は、この要望にこたえるものであって、サフラン
任用成分を含「する柱頭ＦＪ組組織効率的に産生させる
方法、及び、該柱頭様組織からサフラン有用成分を効率
的に製造する方法を提供することを目的とする。The present invention meets this need and provides a method for efficiently producing stigma FJ tissue containing saffron-like components, and a method for efficiently producing saffron useful components from the stigma-like tissue. The purpose is to
本発明は、下記の構成のものである。 The present invention has the following configuration.
（第１発明）
サフランの花柱又は／及び子房を、ベンジルアデニン及
びａ−ナフタレン酢酸を含有する培地で組は培養してサ
フラン柱ＩｖＩ様組織を産生させることを特徴とするサ
フラン柱頭様組織の産生方法。(First invention) A saffron stigma-like tissue, characterized in that saffron stigma IvI-like tissue is produced by culturing saffron styles and/or ovaries in a medium containing benzyladenine and a-naphthalene acetic acid. Production method.
（第２発明）
サフランの花柱又は／及び子房を、ベンジルアデニン及
びα−ナフタレン酢酸を含有する培地で組は培養してサ
フラン柱頭様組織を産生させて得たサフラン柱頭様組織
から、色素等のサフラン有用成分を採取することを特徴
とするナフラノイｒ用成分の製造方法。(Second invention) Saffron stigma-like tissue is produced by culturing saffron styles and/or ovaries in a medium containing benzyladenine and α-naphthalene acetic acid, and pigments, etc. 1. A method for producing a component for nafuranoi r, which comprises collecting a useful component of saffron.
サフラン有用成分は、前述のように、天然産の場合サフ
ランのめしべを構成する柱頭の部分に含よれるが、本発
明によると、本来柱頭とは別異の部分（器官）である花
柱と子房の部分を組織培養することによって柱頭様組織
を効率よく産生させ、この柱頭様組織から色素等のサフ
ラン任用成分を効率よく採取（製造）することができる
。As mentioned above, saffron useful ingredients are contained in the stigma that constitutes the pistil of saffron in the case of natural products, but according to the present invention, the useful components of saffron are contained in the stigma, which is a different part (organ) from the stigma. By tissue culturing the tufts, stigma-like tissue can be efficiently produced, and saffron components such as pigments can be efficiently collected (manufactured) from this stigma-like tissue.
本発明は、いわゆる植物ホルモンに属する特定の物質を
併わせ含有する培地を用い、サフランの特定の器官を明
所又は暗所で組は培養することにより、「色の柱頭様組
織を産生じ増殖させる点に特徴がある。The present invention aims at cultivating specific organs of saffron in the light or in the dark using a medium containing specific substances belonging to so-called plant hormones. It is characterized by the fact that it allows
本発明は、サフランの花柱又は／及び子房を組織培養す
ることによりその目的が達せられる。ここで、サフラン
の花柱及び子房とは、サフラン色素の生産や薬用などに
′Ａ通常用される有色の柱頭とはそれぞれ別異の器官を
意味する。The object of the present invention is achieved by tissue culturing saffron styles and/or ovaries. Here, the saffron style and ovary refer to organs that are different from the colored stigma that is normally used for the production of saffron pigment and for medicinal purposes.
サフランの花柱又は子房以外の器官、例えば柱頭や花茎
といった器官を組織培養した場合、カルスは生成するも
のの、有色の柱頭様組織を産生させることはできない。When organs other than the style or ovary of saffron, such as stigmas and inflorescences, are tissue cultured, callus is produced, but colored stigma-like tissue cannot be produced.
本発明では、サフランの開花前に、すなわち、蕾の伏態
のときに花柱又は／及び子房の部分を取り出し、これを
通常の方法に従って組織培養に付し、柱頭様組織を産生
増殖させる。サフランの開花後に同様な操作を行った場
合は、柱頭様組織を効率的に産生させることはむずかし
い。In the present invention, before flowering of saffron, that is, when the buds are in a hypostatic state, the style and/or ovary are removed and subjected to tissue culture according to a conventional method to produce and proliferate stigma-like tissue. If similar operations were performed after saffron flowering, it would be difficult to efficiently produce stigma-like tissue.
本発明で得られる柱頭様組織の代表的な例について述べ
ると、形杖は、サフランめしべの柱頭にきわめて類似し
ており、色は生成したサフラン色素の含有量に応じて、
黄色から赤色を呈する。Describing a typical example of the stigma-like tissue obtained by the present invention, the cane shape is very similar to the stigma of a saffron pistil, and the color varies depending on the content of the saffron pigment produced.
Appears yellow to red.
本発明で使用できる培地は、組織培養用の基本培地でよ
く、特に格別なものである必要はない。The medium that can be used in the present invention may be a basic medium for tissue culture and does not need to be particularly special.
枦物の組織培養に通常用いられる例えばＭｕｒａｓｈ
ｉｇｅ−５ｋｏｏ（の培地、ｌ！ｈ ｉ ｔｅの培地、
Ｌｔｎｓｍａ＋ｅｒ−５ｋｏｏｇの培地、Ｇａｎｔｈｃ
ｒｅｔの培地、Ｔｕｌｅｃｋｅの培地、Ｍｏｒｅｌの培
地などを使用することができる。なかでもＭｕｒａｓｈ
ｉｇｅ−５ｋｏｏｇの培地が好速である。本発明では
、このような基本培地にベルジルアデニン及びα−ナフ
タレン酢酸を添加して培養を行う。For example, Murash, which is commonly used for tissue culture of
ige-5koo (medium, l!h ite medium,
Ltnsma+er-5koog medium, Ganthc
Ret's medium, Tulecke's medium, Morel's medium, etc. can be used. Especially Murash
ige-5koog medium is fast. In the present invention, culture is performed by adding verzyladenine and α-naphthalene acetic acid to such a basic medium.
本発明において培地に含有させるベンジルアデニンは、
植物ホルモンのサイトカイニン頚に属する物質であり、
また、α−ナフタレン酢酸は植物ホルモンのオーキシン
類に属する物質である。本発明では、この特定の２！ｌ
の物質を併含する培地を使用することにより、本発明の
目的を達成することができる。In the present invention, benzyladenine contained in the medium is
It is a substance that belongs to the cytokinin class of plant hormones,
Further, α-naphthaleneacetic acid is a substance belonging to the auxin class of plant hormones. In the present invention, this specific 2! l
The object of the present invention can be achieved by using a medium containing these substances.
ベンジルアデニンは、通常の組織培養で使用される０度
よりもかなり高′ｃ４度で含有させることが、柱頭様組
織を産生、増殖させるのに一豹効果的である。このベン
ジルアデニンにα−ナフタレン酢酸を併用すると柱頭様
組織の産生が効率的に促進される。Containing benzyladenine at a temperature much higher than 0 degrees Celsius used in normal tissue culture is most effective in producing and proliferating stigma-like tissue. When this benzyladenine is used in combination with α-naphthaleneacetic acid, the production of stigma-like tissue is efficiently promoted.
本発明においてベンジルアデニンの代りにサイトカイニ
ノ類に属する他の物質、例えばカイネチンを用いても、
また本発明においてα−ナフタレ／酢酸の代りにオーキ
シン類に屑する他の物質、例えばインドール−３−酢酸
を用いても所期の目的は達成されない、、′
培地には、本発明の目的を阻害しない限り、他の物質を
適宜含有させてもよい。In the present invention, even if other substances belonging to the cytokinino group, such as kinetin, are used instead of benzyladenine,
In addition, in the present invention, even if other substances that disintegrate into auxins, such as indole-3-acetic acid, are used instead of α-naphthalene/acetic acid, the intended purpose will not be achieved. Other substances may be included as appropriate, as long as they do not interfere.
培地には、ベンジルアデニンを５〜１５ＰＰｍ及びα−
ナフタレン酢酸を ゛ −０，１〜１０ｐ
ｐｍを含有させるのがよい。The medium contains benzyladenine at 5 to 15 PPm and α-
Naphthalene acetic acid ゛ -0,1~10p
It is preferable to include pm.
本発明で行う組織培養の方法としては、例えば固体培地
を用いた静置培養方法でも、また、液体培地を用いたＷ
ｔ盪培養方法であ、でもよい。固体の培地としては、例
えば寒天を約α８ｆｆｉｆｆｉ％含イｒする１Ｊｕｒａ
ｓｈ ｉｇｅ−５ｋｏｏｇの培地などが用いられる。培
養は明所でも、暗所でもよい。培ｆ！温度は１５〜３０
℃、好ましくは２０〜３０℃の範囲で行う。The tissue culture method of the present invention includes, for example, a stationary culture method using a solid medium, and a W method using a liquid medium.
A t-culture method may be used. As a solid medium, for example, 1 Jura containing about α8ffiffi% agar.
A medium such as Shige-5koog is used. Culture may be carried out in the light or in the dark. Culture f! Temperature is 15-30
The temperature is preferably 20 to 30°C.
Ｍｕｒａｓｈ ｉｇｅ−Ｓｋｏｏｇの培地で２５℃、暗
所での培養例では、通常１〜２か刀の経過により柱ｍ様
組織の産生が認められる。こうして産生させた柱頭様組
織を、引続き固体培地又は液体培地で、暗所又は明所に
おいて培養することにより柱頭様組織を増殖させる。When cultured in a Murashige-Skoog medium at 25° C. in the dark, production of pillar-like tissue is usually observed after 1 to 2 hours. The stigma-like tissue produced in this manner is subsequently cultured in a solid medium or liquid medium in the dark or in the light, thereby proliferating the stigma-like tissue.
本発明により増殖した有色の柱頭様組織からサフラン色
素を取り出すには、柱頭様組織をそのまま、又はすりつ
ぶして溶剤、例えば水、含水エタノール、含水プロビレ
／グリコールなどを用いて、常温又は加熱下に抽出すれ
ばよい。In order to extract the saffron pigment from the colored stigma-like tissue grown according to the present invention, the stigma-like tissue is extracted as it is or ground and extracted using a solvent such as water, aqueous ethanol, aqueous propyle/glycol, etc. at room temperature or under heating. do it.
本発明で得られた柱頭様組織を水で抽出して得′γ黄色
の着色液の紫外、可視吸収スペクトルを、パ天然のサフ
ラン柱頭より油出したサフラン色素水溶液の吸収スペク
トルと比較して、本発明で得られた色素が天然のサフラ
ン色素と同等のものであることが確認された。The ultraviolet and visible absorption spectra of a yellow colored solution obtained by extracting the stigma-like tissue obtained in the present invention with water were compared with the absorption spectrum of an aqueous saffron dye solution extracted from natural saffron stigmas. It was confirmed that the pigment obtained in the present invention is equivalent to natural saffron pigment.
本発明によれば、天然栽培を行ってサフラン色素を？！
）る方法に比べ、季節に左右されず、短期間で大１−１
の色素を得ることが可能である。本発明で得られる柱頭
様組織や、これから抽出して得た色素は、食品、化粧品
などの着色や薬用原料として使用できる。According to the present invention, saffron pigment is produced by natural cultivation? !
) method, it is unaffected by the season and achieves 1-1 results in a short period of time.
It is possible to obtain dyes of The stigma-like tissue obtained in the present invention and the pigment extracted therefrom can be used to color foods, cosmetics, etc., and as medicinal raw materials.
実施例Ｉ
Ｍｕｒａｓｈ ｉｇｅ−５ｋｏｏｇの培地に、ベンジル
アデニン及びα−ナフタレン酢酸を、第１表に示す量に
なるようにそれぞれ添加し、これに寒天粉末を０．８
徂ｆｆｉ％となるように加え、オートクレーブ中１２０
℃で２０分１ｊｆｆ殺菌した。得られたｒａ液を直径５
５■−のプラスチック製シャーレに分注し、冷却固化さ
せて培地を調製した。Example I To a Murash ige-5koog medium were added benzyladenine and α-naphthalene acetic acid in the amounts shown in Table 1, and to this was added 0.8 g of agar powder.
120% in the autoclave.
It was sterilized at ℃ for 20 minutes. The obtained RA liquid is made into a diameter of 5
A culture medium was prepared by dispensing the mixture into a 5-inch plastic petri dish and solidifying it by cooling.
一方、サフランの開花前、まだ蕾の時に、めしべ部分を
採取し０．５重量％次亜塩素酸ソーダ水溶液に１０分間
浸漬して殺国し、ついでこれを水洗した後、子房部分を
切り取ってこれを上記の培地に置床した。On the other hand, before flowering, when the saffron is still in bud, collect the pistil, immerse it in a 0.5% by weight sodium hypochlorite aqueous solution for 10 minutes to kill the saffron, wash it with water, and cut out the ovary. This was then placed on the above medium.
これを２５℃暗所で静置培養したところ、ベンジルアデ
ニン及びａ−ナフタレン酢酸の量により、早いものでは
培養後６週間位経つと、柱頭様組織の産生が認められ、
８週間後には、この組織が成長し、かつかなり赤色に着
色しζ、サフラン色素がかなり生成してきた。When this was cultured statically in the dark at 25°C, production of stigma-like tissue was observed as early as 6 weeks after culture, depending on the amount of benzyladenine and a-naphthalene acetic acid.
After 8 weeks, this tissue has grown and is quite red in color, and a considerable amount of saffron pigment has been produced.
柱頭様組織の産生量、色素０度及びサフラン色素の産生
量に関し、それぞれ第１表、第２表及び第３表に示す結
果を得た。The results shown in Tables 1, 2, and 3 were obtained regarding the production amount of stigma-like tissue, 0 degree pigment, and saffron pigment production, respectively.
第１表
（サフラン色素を含む柱頭様組織の見掛けの産生量）注
（鳳） ＢＮ：ベンジルアデニン
α−ＮＡＡ ：α−ナフタレン酢酸
注（２） サフラン色素を含む柱頭様組織の見掛番すの
産生量：天然サフランのサフラン色素を含む柱頭の部分
１本に相当する量を１０、量的にないものを０とし、こ
の間を比例区分してＩＩ段階の量を示す尺度とし、サフ
ランの外植片（天然サフランの子房部分から切取った組
ｉ片であり、これを特定の条件で組織培養することによ
り柱頭様組織が産生じた。）１個当り発生したサフラン
色素を含む柱頭様組織当量を目視によって判定し、上記
尺度に当てζまめて数値とする。Table 1 (apparent production amount of stigma-like tissue containing saffron pigment) Note (Otori) BN: Benzyl adenine α-NAA: α-naphthalene acetic acid Note (2) Apparent production amount of stigma-like tissue containing saffron pigment Production amount: The amount equivalent to one stigma part containing saffron pigment of natural saffron is 10, and the amount that is quantitatively absent is 0, and the proportion between these is divided to indicate the amount of stage II, and saffron explant Piece (This is a piece cut from the ovary part of natural saffron, and stigma-like tissue was produced by culturing this under specific conditions.) Stigma-like tissue containing saffron pigment generated per piece Determine the equivalent weight visually, apply it to the above scale, and calculate the numerical value.
第２表
（サフラン色素を含む柱頭様組織のＱ掛。）の色素濃度
）注（１） ＢＮ：ベンジルアデニン
α−ＮＡＡ ：α−ナフクレン酢酸
注（２） サフラン色素を含む柱頭様組織の見掛けの色
素濃度：天然サフランのサフラン色素を含む柱頭の部分
の色の濃さを１０、無色を０とし、その間をサフラン色
素の４度に比例するように１１段階に区分して色の濃さ
を示す尺度とし、組織培養によって発生したサフラン色
素を含む柱頭様組織の色の濃さを目視によって判定し、
上記尺度に当てはめて数値とする。Pigment concentration in Table 2 (Q factor of stigma-like tissue containing saffron pigment) Note (1) BN: Benzyl adenine α-NAA: α-nafucrene acetic acid Note (2) Apparent density of stigma-like tissue containing saffron pigment Pigment density: The color density of the stigma of natural saffron containing the saffron pigment is 10, colorless is 0, and the color density is divided into 11 levels proportional to the 4th degree of the saffron pigment. As a scale, visually determine the color density of stigma-like tissue containing saffron pigment generated by tissue culture,
The value is calculated by applying it to the above scale.
第３表
（サフラン組織外植片１個当りの見掛けのサフラン色素
の産生ｆｆ１）注（１） ＢＮ：ベンジルアデニン
α−ＮＡＡ ：α−ナフタレ／酢酸
注■ サフラン組織外植片１個当りの見掛けのサフラン
色素の産生Ｓ１：天然サフランのサフラン色素を含む柱
頭の部分１本中に含まれるサフラン色素量に相当する量
を１００、量的にないものを０とし、この間を比例区分
して１１段階の量を示す尺度とし、組織培養によりサフ
ランの外植片１個当り発生した色素の産生量を、第１表
及び第２表を参照しながら目視によって判定し、上記尺
度に当てはめて数値とする。Table 3 (apparent saffron pigment production ff1 per saffron tissue explant) Note (1) BN: Benzyl adenine α-NAA: α-naphthalene/acetic acid Note ■ Apparent production per saffron tissue explant Production of saffron pigment S1: The amount corresponding to the amount of saffron pigment contained in one stigma part of natural saffron containing saffron pigment is set as 100, and the amount that is not present is set as 0, and the proportion is divided into 11 stages. The amount of pigment produced per saffron explant through tissue culture is determined visually with reference to Tables 1 and 2, and the value is calculated by applying it to the above scale. .
実施例２
実施例１でサフランの子房部分を培養する代りに、蕃か
ら採取した花柱部分を用いて同様な条件で実験を行った
ところ、同様な結果を得た。Example 2 Instead of culturing the ovary of saffron in Example 1, an experiment was conducted under the same conditions using the style collected from the bush, and similar results were obtained.
また、花柱部分を培養する場合は、子房部分を培養する
場合に比して、ベンジルアデニンとα−ナフタレン酢酸
とをより高濃度に存在させた方が良好な結果が得られた
。Furthermore, when culturing the style part, better results were obtained when benzyladenine and α-naphthalene acetic acid were present at higher concentrations than when culturing the ovary part.
比較例１
実施例１において、α−ナフタレ／酢酸を用いる代りに
インドール−３−酢酸を用い、この他は実施例１と同様
にして培養を試みたところ、器官分化は起らず、サフラ
ン色素は生成しなかった。Comparative Example 1 When culture was attempted in the same manner as in Example 1 except that indole-3-acetic acid was used instead of α-naphthalene/acetic acid, organ differentiation did not occur, and saffron pigment was not generated.
比較例２
実施例１においてベンジルアデニンを重用する以外は実
施例と同様に実験を行ったところ、柱頭様組織はわずか
じか産生することができず、サフラン色素の産生量は実
施例１に比較して明らかに少なかった。Comparative Example 2 An experiment was carried out in the same manner as in Example 1, except that benzyladenine was heavily used. As a result, only a small amount of stigma-like tissue could be produced, and the amount of saffron pigment produced was compared to that in Example 1. It was clearly less.
比較例３
実施例１においてα−ナフタレン酢酸を単用する以外は
実施例１と同様に実験を行ったところ、柱頭様組織を産
生ずることができなかった。Comparative Example 3 When an experiment was carried out in the same manner as in Example 1 except that α-naphthaleneacetic acid was used alone, no stigma-like tissue could be produced.
Claims (4)
ン及びα−ナフタレン酢酸を含有する培地で組織培養し
てサフラン柱頭様組織を産生させることを特徴とするサ
フラン柱頭様組織の産生方法。(1) A method for producing saffron stigma-like tissue, which comprises culturing saffron styles and/or ovaries in a medium containing benzyladenine and α-naphthalene acetic acid to produce saffron stigma-like tissue.
レン酢酸０．１〜１０ｐｐｍ を含有する培地である特許請求の範囲（１）の産生方法
。(2) The production method according to claim (1), which is a medium containing 5 to 15 ppm of benzyladenine and 0.1 to 10 ppm of α-naphthalene acetic acid.
ニン及びα−ナフタレン酢酸を含有する培地で組織培養
してサフラン柱頭様組織を産生させて得たサフラン柱頭
様組織から、色素等のサフラン有用成分を採取すること
を特徴とするサフラン有用成分の製造方法。(3) Saffron stigma-like tissue is obtained by culturing saffron styles and/or ovaries in a medium containing benzyladenine and α-naphthalene acetic acid to produce saffron stigma-like tissue, which is useful for producing saffron pigments, etc. A method for producing saffron useful ingredients, which comprises collecting the ingredients.
製造方法。(4) The manufacturing method according to claim (3), wherein the production method is obtained by solvent extraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62074949A JPH062055B2 (en) | 1987-03-28 | 1987-03-28 | Method for producing saffron stigma-like tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62074949A JPH062055B2 (en) | 1987-03-28 | 1987-03-28 | Method for producing saffron stigma-like tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63240782A true JPS63240782A (en) | 1988-10-06 |
| JPH062055B2 JPH062055B2 (en) | 1994-01-12 |
Family
ID=13562088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62074949A Expired - Lifetime JPH062055B2 (en) | 1987-03-28 | 1987-03-28 | Method for producing saffron stigma-like tissue |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH062055B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532465A (en) * | 2016-01-09 | 2016-05-04 | 佛山市金蓝领教育科技有限公司 | Induction culture medium for inducing budding of saffron crocus corm and induction method of induction culture medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62275617A (en) * | 1986-02-04 | 1987-11-30 | 味の素株式会社 | Female pith of crocus sativas l and its production |
| JPS63109788A (en) * | 1986-06-06 | 1988-05-14 | Ajinomoto Co Inc | Production of safranal, crocin or analog thereof |
| JPS63258574A (en) * | 1986-09-20 | 1988-10-26 | Ota Isan:Kk | Culture of safron stigma tissue |
-
1987
- 1987-03-28 JP JP62074949A patent/JPH062055B2/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62275617A (en) * | 1986-02-04 | 1987-11-30 | 味の素株式会社 | Female pith of crocus sativas l and its production |
| JPS63109788A (en) * | 1986-06-06 | 1988-05-14 | Ajinomoto Co Inc | Production of safranal, crocin or analog thereof |
| JPS63258574A (en) * | 1986-09-20 | 1988-10-26 | Ota Isan:Kk | Culture of safron stigma tissue |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532465A (en) * | 2016-01-09 | 2016-05-04 | 佛山市金蓝领教育科技有限公司 | Induction culture medium for inducing budding of saffron crocus corm and induction method of induction culture medium |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH062055B2 (en) | 1994-01-12 |
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