JPS63214176A - Plate for identification of microorganism - Google Patents

Abstract

PURPOSE:To satinize the lower part of the wall inner surface of the ponds to expand the contact area and allow the culture medium for identification to fast to the pond wall surfaces in the plate for microorganism identification having a plurality of ponds formed. CONSTITUTION:A plurality of ponds 2 for receiving the culture medium for microorganism identification are formed on the main body 1 of a substantially square plate so that their openings are positioned on the same surface of the main body. Further, at least the lower parts of wall inner surface 14 of individual ponds are finished into satin-embossed surface 13. Then, different kinds of culture media for identification is charged into individual pond bottoms 11 depending on the inspection items and adhered to the satinized surfaced tightly. The test solutions containing a microorganism are poured into the pond bottoms 11 to observe the reactions of the microorganism with the media and identify totally the microorganism. The satinization of the pond wall inner surface 13 can prevent the culture media from peeling off.

Description

Detailed Description of the Invention (19) Background of the Invention (Technical Field) The present invention relates to a microorganism identification plate suitably used for identifying microorganisms such as intestinal bacteria.

(Prior art and issues) In hospitals, etc., in order to take appropriate measures against infectious diseases, pathogens are identified, susceptibility tests are conducted to find effective drugs, and appropriate doses are administered to patients. ing. To perform a pathogen identification test, usually blood, urine, feces, isthmus, cerebrospinal fluid, etc. are collected from a patient, the whole sample or a portion thereof is suspended in sterile water, etc., and a portion of this suspension is collected. The cells are uniformly inoculated onto a suitable medium such as agar medium, and the bacteria are cultured.
Select ~2■ and suspend it in sterile water, etc. to prepare an identification test solution.Next, this test solution is placed in a microorganism identification device called a microplate, which contains various test media in advance. The cells are then dispensed into each well, the reactivity to each medium is determined, and the results are combined to identify the bacteria.

Conventional microplates used to identify such microorganisms have a flat or concave bottom surface for each hole, so in order to make it easier to judge test results, the bottom surface of each hole must be covered. It is necessary to store the culture medium, which requires a large amount of culture medium and a correspondingly large amount of test solution (
For example, it may be necessary to dispense 0.15 to 0.2 l).

Most of each test reaction requires oxygen from the air, but when there is a large amount of test liquid, the contact rate with air is low, and as a result each test reaction takes a long time (e.g., 18 to 24 hours).
Therefore, it was impossible to identify it on the same day.

Therefore, in order to improve this problem, the bottom surface of the hole in the microborate is made into a convex spherical shape, and the top part is removed <rt! It has been proposed that a circular culture medium for identifying microorganisms is fixed to the 41& part (% Publication No. 58-20263).
With this microplate, microorganisms can be identified with a small amount of medium for identifying microorganisms and a small amount of test solution for identification. However, since this culture medium is in narrow contact with the plastic microplate plate, which has a smooth surface and poor adhesion, there was a risk that the above-mentioned annular culture medium would crack and peel off.

(1) Purpose of the Invention The object of the present invention is to provide a microorganism identification plate to which a microorganism identification medium can be firmly fixed.

The above objects are achieved by the microorganism identification plate of the present invention described in each section below.

(1) * A plurality of bottomed cylindrical bodies for accommodating test solutions for biological identification are provided in the plate body with their openings located on the same surface as the surface of the plate body. 1. A microorganism identification plate, characterized in that the bottomed cylindrical body has a matte finish at least at the lower part of the inner peripheral side wall thereof, and a microorganism identification medium is fixed to the matte finish.

(2) The satin-like portion has a height of H1 to 100 gm.

2. The plate for microorganism identification according to item 1, characterized in that it is composed of unevenness with a pitch of P1 to 100 jLm.

(3) The microorganism identification plate according to item 1 or 2, wherein the built-up portion is provided only at the lower part of the circumferential wall of the cylindrical body.

By using the microorganism identification plate of the present invention having the above structure, the microorganism identification medium can be firmly fixed.

10. Detailed Description of the Invention The configuration of the invention will be described in detail below based on an embodiment shown in the drawings.

FIG. 1 shows a plan view of the microorganism identification plate of the present invention. This microorganism identification plate has a rectangular plate body 1 with holes 1 and 11 formed in accordance with the inspection items. A plurality of bottomed cylindrical bodies (wells) to be formed, such as inverted truncated cones 2 (18 in the figure), are spaced apart.

As shown in FIG. 2, the cylindrical body 2 has an opening slightly protruding from the surface of the plate body 1, but is actually located on the same plane as the surface of the plate body 1. The bottom surface 3 is configured to be planar. Most cylindrical bodies used for test reactions that require oxygen in the air to undergo a process are 1
For example, the cylindrical body has an opening inner diameter of 7 mm, a bottom inner diameter of 4.5 am, and a depth of 11 mm, but is suitable for test reactions conducted in relatively anaerobic conditions such as hydrogen sulfide production reactions and indole production reactions. For example, the opening inner diameter is 8 mm, the bottom inner diameter is 3.5 layers, and the depth is 12 mm, which is narrow and deep. Further, a cylindrical body for use in testing pressure applications that requires a relatively large amount of oxygen, such as the citric acid decomposition reaction, is formed to be wide and shallow, for example, with an opening inner diameter of 7 mm, a bottom inner diameter of 5 mm, and a depth of 1θ.

The cylindrical bodies 2 are arranged in rows, as shown in FIG. The surface portion 4 of the plate body 1 at a position corresponding to each cylindrical body 2 in the direction is marked with a symbol etc. (i.e.
For example, + and - can be written on the surface to indicate positive or negative for each test reaction (letters, figures, symbols). Such a surface 4 can be achieved by making it satin-like. In addition, in the figure, band-shaped satin-like parts 5 and 6 are also installed at the upper and lower end portions of the plate main body 1, and the patient name,
You can enter the sample name and other necessary information.

Further, between each satin-like surface 4 and each cylindrical body 2, an abbreviation indicating the test reaction or substrate name to be tested on each cylindrical body 2 is attached.

In the figure, GLC is the glucose decomposition reaction, NIT is the nitrate reduction reaction, and LYS is the decarboxylase reaction (substrate:
lysine hydrochloride), ORN is decarboxylase reaction (substrate: ornithine hydrochloride), SOR is sorbitol decomposition reaction, MAN is mannitol decomposition reaction, ARA is 7-rabinose decomposition reaction, ) (S is hydrogen sulfide production reaction, IND is indole production reaction, URE is urease reaction, ARG is decarboxylase reaction (substrate: arginine hydrochloride), SA
C is saccharose decomposition reaction, RAF is raffinose decomposition degree wide, RHA is rhamnose decomposition reaction, MALO is malonic acid decomposition reaction, TDA is indolepyruvate production reaction (
deaminase reaction), vp is the 7 setoin production reaction (Vogesbroskaer reaction), CIT is the citric acid decomposition reaction, INO is the inosit decomposition reaction, ADO is the atonite decomposition reaction, and 0NPG is the β-galactosidase reaction (substrate: orthonitrophenyl). β-D-galactopyranoside).

The plate for identifying microorganisms shown in the figure has a peripheral wall 7 that is connected to the peripheral end of the plate body 1 and surrounds the cylindrical body 2.
is formed. This peripheral wall 7 is a cylindrical body as shown in FIG. An annular rib 8 extending outward in a direction parallel to the surface of the plate body 1 and an annular protrusion 9 extending downward from the peripheral end of the annular rib 8 are formed at the lower end thereof. ing. In this way, when the plates are stacked, the upper plate acts as a lid, and the upper end surface of the lower plate engages with the inner wall of the step formed by the annular rib 8 and the annular protrusion 9 of the upper plate and moves. Such plates can be made of plastic, which is convenient for transport and storage. Note that another lid may be provided at the top of the plate.

The bottom surface 3 of the cylindrical body 2 is formed into a flat shape. Preferably, in order to increase the specific surface area in contact with air, the amounts of the various biochemical property test media 10a to 10r and the test liquid for microorganism identification required for microorganism identification are made as small as possible.
It may be formed into a spherical surface projecting upward, and the lower part of the inner circumferential wall surface 14 of this cylindrical body 2 is subjected to a satin finish.

This satin-like surface 13 has microorganism identification media 10-a to 10.
This is to improve the adhesion of R to prevent peeling after the bag is dried, and is considered necessary at least on the side wall of the surface to which the culture media 10a to 10r are to be adhered. This satin-like surface 13 scatters transmitted light and makes it difficult to mechanically read the change in color tone of the test liquid as a change in absorbance from the outside. It is not preferable to form it on the surface of the body 2, especially in the center of the side surface. If it is provided only at the bottom of the surface, it is preferable because it does not cause scattering of the transmitted light and does not interfere with mechanically reading the color tone change of the test liquid. The satin-like surface 13, which may be provided at the corner of the Taisei 3, has a height of, for example, H1 to tQ (lpm).

It is preferable to have an uneven surface with a pitch of P1 to 100 layers.If the height and pitch are less than 11, peeling of the culture medium cannot be prevented much when the surface is close to a smooth surface, and furthermore, if the height and pitch are less than 11,
If it is 0 p or more, the number of convex portions becomes too small, which is not preferable. More preferably, the height H is about 5 to 501 Lm, and the pitch P is about 5 to 50 Lm.

As shown in FIG. 1, a microorganism identification medium 10a-1Or suitable for each test reaction is fixed in an annular shape to the periphery of the matte surface 13 of the bottom surface 3 of each cylindrical body 2. There is. Such a circular medium.

It can be formed by dispensing each liquid culture medium into each cylindrical body 2 until it covers the periphery of the bottom surface 3 and drying it with hot air in a short time. As this medium, any of the conventionally known test reaction media can be used.

To identify microorganisms using this microorganism identification plate described above, a drop of a test solution prepared by a conventional method is dispensed into each cylindrical body 2 using a dispensing dropper.1 For example, intestinal bacteria can be identified. If so, culture at 35°C to 37°C for 4 to 5 hours. Each culture medium 10a fixed to each cylindrical body 2 in an annular manner
Since the amount of 10r is smaller than conventional ones, it is easily dissolved and various reactions occur. Therefore, according to the existing judgment table for microorganism identification, each cylindrical body is
+ or - on the satin-like surface 4 of the plate body 1 corresponding to
Enter. Microorganisms are identified by comprehensively judging the + and - indications.

■0 Different functions and effects of the invention As is clear from the above explanation, the microorganism identification plate of the present invention has a plurality of bottomed cylindrical bodies with openings for accommodating the microorganism identification test liquid. In the microorganism identification plate provided on the plate main body so as to be located substantially on the same surface as the surface of the plate main body, at least the lower part of the inner circumferential side wall of the bottomed cylindrical body has a satin finish, and the microorganisms are placed in the satin finish part. Since the identification medium is fixed, compared to conventional microplates, the contact area per unit volume between the microorganism identification medium and the cylindrical body is significantly increased, and the engagement of fine irregularities increases the physical adhesion force. is increased, and peeling of the medium after drying can be prevented. Of course, if the bottom surface of the cylindrical body of the microorganism identification plate is made into a convex spherical surface, the effect achieved by fixing the culture medium in a circular shape around the periphery excluding the top, that is, the amount of culture medium and the amount of test solution can be reduced. It goes without saying that the effect of microorganism identification, such as the time it takes to identify microorganisms, is not impaired.
The fixing effect of the dry medium is greatest when applied at a final weight of ~100 and a pitch of P1 to 100 l.

[Brief explanation of the drawing]

FIG. 1 is a plan view showing an embodiment of the microorganism identification plate according to the present invention, FIG. 2 is a sectional view taken along the line ■-■ in FIG.
The figure is an enlarged sectional view of the satin-like surface. 3 to 5 are enlarged sectional views of the bottom corner of the cylindrical body. DESCRIPTION OF SYMBOLS 1... Plate main body, 2... Cylindrical body, 3... Bottom surface 7a... Inner peripheral wall surface of cylindrical body, loa~lor... Medium for microorganism identification, 11... Hole, 12 ... Corner, 13... Satin-like surface. 1 patentee Terumo Corporation

Claims (3)

[Claims] (1) For microorganism identification, a plurality of bottomed cylindrical bodies for accommodating test solutions for microorganism identification are provided in the plate body with their openings located on substantially the same surface as the plate main body surface. A plate for identifying microorganisms, characterized in that at least a lower portion of the inner peripheral side wall of the bottomed cylindrical body has a satin-like texture, and a culture medium for identifying microorganisms is fixed to the satin-like part. (2) The plate for microorganism identification according to claim 1, wherein the satin-like portion is composed of unevenness with a height of H1 to 100 μm and a pitch of P1 to 100 μm. (3) The microorganism identification plate according to claim 1 or 2, wherein the satin-like portion is provided only at the lower part of the circumferential wall of the cylindrical body.