JPS5820263B2 - Microorganism identification plate - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION ■ Background Technical Field of the Invention The present invention relates to a plate used for identifying microorganisms such as intestinal bacteria.

Prior Art and Problems In hospitals, etc., in order to take appropriate measures against infectious diseases, microorganisms such as pathogenic bacteria are identified, susceptibility tests are performed to find effective drugs, and the appropriate amount of the drug is administered to patients. ing.

To perform a pathogen identification test, usually blood, urine, feces, cough, spinal fluid, etc. are collected from a patient, the whole sample or a portion thereof is suspended in sterile water, etc., and a portion of this suspension is diluted. The cells are inoculated in a streak pattern onto an appropriate medium such as agar medium, and the bacteria are cultured.

Colonies with a diameter of 1 to 21 nm are selected from the colonies thus obtained and suspended in sterile water to prepare an identification test solution.

Next, this test solution is dispensed into each hole of a so-called microplate containing various test media, the reactivity to each medium is measured, and the measurement results are combined to identify the bacteria. .

Conventional microplates used to identify such microorganisms have a flat or concave bottom surface for each hole, so in order to make it easier to distinguish the test results, the bottom surface of each hole should be covered. It is necessary to accommodate a medium, which increases the overall amount of medium required.

In response, it is necessary to dispense relatively large amounts (eg, 0.15 to 0.2 ml) of test fluid into each well of conventional microplates.

Each test reaction requires mostly oxygen from the air, whereas this large amount of test liquid reduces the rate of contact with air, resulting in each test reaction taking a long time (e.g., 18 to 24 hours).
Therefore, it was impossible to identify it on the same day.

(1) Purpose of the Invention The purpose of the present invention is to provide a microorganism identification plate that allows microorganisms to be identified relatively quickly.

According to the present invention, there is provided a plate body, and a plurality of tubes for accommodating a test liquid for identifying microorganisms, which are spaced apart from each other within the plate body so that the open end thereof is located substantially on the same plane as the surface of the plate body. Provided is a microorganism identification plate comprising a bottomed cylindrical body whose bottom surface has a convex curved surface, and a microorganism identification medium annularly fixed to the periphery of the bottom surface of the cylindrical body. .

Preferably, the cylindrical body is disposed externally, and the surface portion of the plate body at a position corresponding to the cylindrical body in the row direction is a surface on which symbols and the like can be written.

Further, in this case, it is preferable to arrange the cylindrical bodies in three rows.

In addition, as a culture medium, Labremco meat extract powder 0.01 to 0.9 g/l water, peptone for bacteria 0.1 to 9 g/l.
0g/11 water, glucose 11.0 to 19. Og/
l water, ammonium nitrate 0.1 to 5.0! ! /1 water, sodium bisulfite 0.01 to 5.0 g/l!
Water, sodium bicarbonate 0.1 to 20.0 g/l water and phenol red 0.05 Water, 0.5
Medium consisting of g/l water composition, Labremco meat extract powder 0
．． 01 to 0.9 g/l water, bacterial peptone o, i to 9.0 g/l water, arabinose 11,0 to 19
．． 0g/l water, sodium bisulfite 0.1 to 5.
09/l water, sodium bicarbonate 0.1 to 20.0
g/l water and phenol red 0.05 to 0.5
Medium consisting of 9/l water composition, Labremco meat extract powder 0
．． 01 to 0.9 g/l water, bacterial peptone 0.1 to 9.0 g/l water, rhamnose 11.0 to 19. (
J9/l water, sodium bisulfite 0.01 to 5.
0g/l water, sodium bicarbonate 0.01 to 20.
0 g/l water and phenol red 0.05 to 0.
A medium with a composition of 5 g/l water, 0.01 to 1.0 g of Labremco meat extract powder, Q water, and 0.0 g of peptone for bacteria.
1 to 1 o, l/IJ water, disodium hydrogen phosphate 0.1 to 3.0.0 g/l water and sorbitol,
A medium consisting of 1 to 5% of saccharose, inosit, mannitol, raffinose or attonit;
Tryptone TO91-50, og/V water, orthonitrophel-β-D-galactopyranoside 0.5-5.0 g/l water, glucose 0.005.9-5.
0g/11 water, sodium bisulfite 0.01g to 0.5g/l water and 0.1 disodium hydrogen phosphate
A medium with a composition of 20.0 to 20.0 g/l water, liver extract powder 0.01.9/l or more, yeast extract powder 0.01 g
/l water or more, tryptone 0.01 g/l water or more, proteose peptone 0.01 g/l water or more, Sawyer peptone 0.01 g/l water or more, sodium thiosulfate l) rum 0
．． 1 g to 5.0 g/L water, 0.1 g to 5.0 g/It water ammonium iron citrate, 0.01 illll water intake of L-1-liptophan, and 0.0 g dipotassium phosphate.
Medium with a composition of 1 to 20.09/l water, protease peptone 0.1 to 20.09/11 water, sodium malonate 5.0 to 3 og/l! Water, L-
Tryptophan 0.01 to 1o, og/A water, glucose 0.01 to 5.0g/l water, sodium chloride 0 to 5.0g/l water, sodium dihydrogen phosphate 0
．． 1 or 2o, Oj! /V water and bromothymol blue 0.05 to 0.5 g/lj water, peptone for bacteria 0.1 to 6.0 g/l water, soya peptone 0.1 to 4.0 g/l water, Yeast extract powder 0.1 to 5.0 g/13 water, glucose 0.1 to 30.0.9/l water, sodium pyruvate 0.1
or 15. (L9/A water, sodium bisulfite 0.
0.01 to 5.0 g/l water, 0.01 to 10.0 g/l water and creatine 0.01 to 10.0 g/l water, sodium dihydrogen phosphate.
Culture medium consisting of a composition of 01 to 5.09711 water, tryptone TO, 01 to 20.0 g/I! , water, L-lysine hydrochloride 5.0 to 50.0 g/V water, glucose 0.01 g to 5.0 g/l water, sodium dihydrogen phosphate 0.01 to 2 o, o9. #Medium consisting of water and bromothymol blue 0.05 to O, F4/l water, tryptone T 0.01 to 20.0 g/l
l water, L-ornithine-hydrochloride 5.0 to 5 o, og
/A water, glucose 0.01g to 5.0g/11 water, sodium dihydrogen phosphate 0.01 to 20.0g
/11 Water and Bromthymol Blue 0.05 to 0
．． Medium consisting of 5g/11 water composition, Kaciton 0.01
5. to 30.0 g/l water, L-arginine hydrochloride.
0 to 50. Oj;//l water, glucose 0.01 to 5.0 g/, 11 water, sodium dihydrogen phosphate 0
．． medium consisting of 0.01 to 20.0 g/l water and bromothymol blue 0.05 to 0.5 g/l water, yeast extract powder 0.01 to 3.0 g/l water, sodium citrate 0.5 to 2 o , l/l water, glucose 0.005 to 0.1 g/l! Water, reduced glutathione 0.01 to 10.0 g/V water, nicotine adenine dinucleotide 0.005 to 5.09/l sodium chloride 0.5 to 10.0 g/11 water, phosphoric acid-
Disodium hydrogen 0.01 to 20. Og/l water and Bromthymol Blue 0.05 to 0.5g/11
Culture medium consisting of water and bacterial peptone o, i to 10.0 g/l water, urea 0.1 to 20.0 g/l
11 water, glucose 0.01 to 5.0 g/11 water,
Sodium chloride O.0.1 to 10.0 g/l water, disodium hydrogen phosphate 0.005 to 50.0 g/l
7 water and phenol red 0.05 to 0.59/
Each test reaction can be performed more quickly by using a medium selected from the group consisting of a medium having a composition of 1 water.

It is also preferable to form a peripheral wall that surrounds the cylindrical body so as to be continuous with the peripheral end of the plate body, and to provide a protrusion that projects laterally outward from the lower end of this peripheral wall.

■Detailed Description of the Invention The present invention will be specifically described below with reference to the accompanying drawings.

As shown in FIG. 1, the microorganism identification plate 10 according to the present invention has a roughly square plate main body 11, and a plurality of microorganisms (18 in the figure) according to the test items are contained in the plate main body 11. A cylindrical body, for example, an inverted truncated conical body 12, is provided at intervals.

This cylindrical body 12 stores a liquid for inspection in a portion.

As shown in FIG. 2, the cylindrical body 12 has an open end slightly protruding from the surface of the plate body 12, but is located substantially on the same plane as the surface of the plate body 12, and its bottom surface 13 constitutes a convex curved surface.

Most cylindrical bodies used for test reactions that normally require oxygen in the air have an opening inner diameter of 7 mm and a bottom inner diameter of 4 mm.
．． 5 mrrt and a depth of 11 mm, but a cylindrical body for test reactions conducted in relatively anaerobic conditions such as hydrogen sulfide production reactions and indole production reactions has an opening inner diameter of 6 mm, a bottom inner diameter of 3.5 gm, and 12m deep
rn, narrow and deep.

Further, a cylindrical body for a test reaction that requires a relatively large amount of oxygen, such as a citric acid decomposition reaction, is formed to be wide and shallow, for example, with an opening inner diameter of 7 cages, a bottom inner diameter of 5 mm, and a depth of 10 mm.

Returning to FIG. 1, the cylindrical bodies 12 are arranged in rows as shown, and in the figure, 18 cylindrical bodies 12 are arranged in three rows.

The surface portion 14 of the plate body 11 at a position corresponding to each cylindrical body 12 in the row direction of each cylindrical body row is marked with a symbol, etc. (i.e., a letter, a figure, a symbol), for example, a positive test reaction.
There is a surface where + and - can be written to indicate negative.

Such a surface 14 can be achieved by having a satin finish.

In addition, in the figure, there is also a band-shaped satin area 1 at the upper and lower ends.
5 and 16 are provided so that the patient name, sample name, and other necessary information can be entered.

Further, between each matte surface 14 and each cylindrical body 12, an abbreviation indicating the test reaction or substrate group to be tested on each cylindrical body 12 is provided.

In the figure, GLC is a glucose decomposition reaction, NIT is a nitrate reduction reaction, LYS is a decarboxylase reaction (substrate: lysine hydrochloride), and ORN is a decarboxylase reaction (substrate: lysine hydrochloride).
ornithine hydrochloride), SOR is sorbitol decomposition reaction, M
AN is mannitol decomposition.

Reaction, ARA is arabinose decomposition reaction, H2S is hydrogen sulfide production reaction, IND is indole production reaction, URE is urease reaction, ARG is decarboxylase reaction (substrate: arginine hydrochloride), SAC is saccharose decomposition reaction, RAF is raffinose decomposition reaction , RHA is rhamnose decomposition reaction, MALO is malonic acid decomposition reaction, TI) A is indolepyruvate production reaction (deaminase reaction), V
P is the acetoin production reaction (Forgesproskaukare reaction), CIT is the citric acid decomposition reaction, INO is the inosit decomposition reaction, ADO is the attonite decomposition reaction, and 0NP
G represents β-galactosidase reaction (substrate: orthonitrophenyl-β-D-galactopyranoside) (the same applies hereinafter).

The microorganism identification plate 10 shown in the figure includes a plate body 1.
A peripheral wall 17 that extends slightly outward and surrounds the cylindrical body 12 is formed so as to be continuous with the peripheral end of the cylinder 1 .

As clearly shown in FIG. 2, this peripheral wall 17 extends below the cylindrical body 12, and has a plate main body 1 at its lower end.
An annular rib 18 extending outward in a direction parallel to the surface of the annular rib 18 and an annular protrusion 19 extending downward from the circumferential end of the annular rib 18.
is formed.

In this way, when the plates 10 are stacked, the upper plate serves as a lid, and the upper end surface of the lower plate engages and moves with the inner wall of the step formed by the annular rib 18 and annular protrusion 19 of the upper plate. It is convenient for transportation and storage as there is no need to do anything.

Such plates can be made of plastic.

Note that another lid body may be provided at the top of the plate.

Now, as clearly shown in FIG. 1, around the bottom surface 13 of each cylindrical body 12, microorganism identification media 20a to 20r suitable for each test reaction are fixed in an annular shape.

In such a circular medium, each medium is placed in each cylindrical body 12.
It can be formed by dispensing the liquid until it covers the periphery of the bottom surface 13 and drying it with hot air in a short period of time.

As this medium, any of the conventionally known culture media for test reactions can be used, but the conventional culture media for various test reactions lack stability and require a relatively long time for the reaction.

In view of this point, the inventors conducted various studies and developed the following improved culture media for various test reactions.

Among the compositions, the left column shows the amount of ingredients used in the examples, and the right column shows the range of ingredients.

■ Improved culture medium for glucose decomposition reaction (GLC) Labremco meat extract powder 0.5 g (0.01-0.9 g) Peptone for bacteria 3.0 g (0.1-9.0 g
) Glucose 15. Og(11,0~19
．． 0 g) ammonium nitrate 0.7 g (0,1
~5.0 g) Sodium bisulfite 0.1 g (0
,01~5.0 g) Sodium hydrogen carbonate 1.1g
(0.1-20.0 g) Phenol red 0.
22 g (0.05-0.5 g) Purified water 2, Improved medium for arabinose decomposition reaction (ARA) Labremco meat extract powder 0.5 g (0.01-0.9 g
) Peptone for bacteria 3.0.9 (0,1-9.
0 g) Arabinose 15.0.9 (11,0
~19.09) Sodium bisulfite 1.0 g
(0.1-5.0 g) Sodium hydrogen carbonate 0.9
g (0.1-20.0 g) Phenol red
0.22g (0.05-0.5g) purified water
1 l 113 Improved medium for rhamnose decomposition reaction (RHA) Labremco meat extract powder 0.5 g (
0.01-0.9 g) Peptone for bacteria 3.0
g (0.1-9.0 g) Rhamnose 1
5.1g (11.0-19.0 g) Sodium bisulfite 0.1,? (0.01-5.0 g) Sodium hydrogen carbonate 0.2 g (0.01-20.09
) Phenol red 0.22g (0.05~0
．． 5.9) Purified water Ill?
1 14, sorbitol (SOR), saccharose (SA
C), Inojit (INO), Mannit (MAN),
Rhinose (RAF), Atonit (ADO), improved medium for each degradation reaction Basic medium composition Labremco meat extract powder 1.0 g (0.01-1
．． 0.9) Peptone for bacteria 10.0 g (0,
1-1o, o 9 ) Phosphate-hydrogen disodium 3.0
g (0,1~vo,o g) phenol red
0.17 g (0.05-0.5 g) purified water
1 l 1 l Add 1 to 5% of each ear of rice to the above basic medium.

5. Improved medium for β-galactosidase reaction (ONPG)
Tryptone T 10. Og(0,1~50
．． 0 g) ONPG* 1.5 g (0
,5-5.0 g) Glucose 0.1
g (0,005-5.0g) Sodium bisulfite
0.01-0.5g phosphate-hydrogen disodium 3
．． 0.9 (0.1-20.0 g) Purified water
17 17*0NPG:, t-lutonitrophenyl-β-D-galactosidase 6, improved medium for hydrogen sulfide production reaction and India step production reaction (H2S/IND) Liver extract powder 3.0 g (0.01 g or more) Yeast Extract powder 3.0.9 (0,01,
9 or more) Tryptone 15.0.9 (0,
01g or more) Proteose Peptone 10.09 (0
,01g or more) Soya Peptone 5.0g (
0.01g or more) Sodium thiosulfate 0.5.9
(0.1-5.0 g) Iron ammonium citrate
0.5 g (0.1-5.0.9) L-tryptophan 1.0 g (0.01 g or more) Dipotassium phosphate 3.0 g (0.1-20.0 g) Purified water
1 l 117, improved medium for malonic acid decomposition reaction and tryptophan deaminase reaction (M
ALO-TDA) Proteose peptone 3.0 g (0.1-20.
0 g) Sodium malonate 10.0 g (5,0
~30.0 g) L-tryptophan 1.5 g (
o, o 1-10.0g) Glucose
0.2 g (0.01-5.0 g) sodium chloride
01.0.9 (0-5.0 g) dihydrogen phosphate-
Sodium 5.5 g (0.1-20.0 g) Bromthymol blue 0.16 g (0.05-0.5 g)
Purified water 1 l 118, Voges Proscalael reaction improved medium (VP) Peptone for bacteria 5.0 g (0.1-6.0.9)
Soya peptone 3.0 g (0.1-4.
0.9) Yeast extract powder 1.0 g (0,
1-5.0.9) Glucose 8.0.
9 (0,1-30.0 j!) Sodium pyruvate 2.0 g (0,1-15.0 g) Sodium bisulfite 0.3.9 (0,01-5.0 g)
Sodium dihydrogen phosphate 0.2.9 (0.01~1
0.0g) Creatine 0.5g (0,
01-5.0.9) Purified water 16
11 9, Improved medium for lysine decarboxylase reaction (LYS
) Tryptone T 2.5g (0.01~20
．． 0 g) L-lysine-hydrochloride 20.0 g (5,0
~50.0 &) Glucose 0.5
g (0.01-5.0 g) Sodium dihydrogen phosphate 0.5 g (0.01-20.0 g) Bromthymol blue 0.12 g (0.05-0.5 g) Purified water
IA? lA10, improved medium for ornithine decarboxylase reaction (ORN) Tryptone T 2.5g (0.01~2o
, Og) L-ornithine-hydrochloride 15.0 g (5,
0-50.0 g) Glucose 0.5
g (0.01-5.0 g) Sodium dihydrogen phosphate 0.5 g (0.01-20.0 g) Bromthymol blue 0.08.9 (0.05-0.5 g) Purified water
1 l 1111, improved medium for arginine decarboxylase reaction (ARG) Kashiton 10. Og(0,01~30.
0g) L-arginine hydrochloride 30.09 (5,0
~50.0 g) Glucose 0.5 g
(0.01-5.0.9) Sodium dihydrogen phosphate 0.8.9 (0.01-20.0g) Bromthymol blue o, 12g (0.05-0.5g) Purified water
1 l l 112, improved medium for citric acid decomposition reaction (CIT) yeast extract powder 1.
0 g (0,01-3.0 g) sodium citrate
6.0 g (0.5-20.0 g) glucose
0.05. ! 9 (0,005~0.1.9
) g) Retathione, reduced form 3.09 (0,01~
10.0g) NAD*0.05g (0,005-5.0
g) Sodium chloride 4.0 g (0.5-1
0.0 g) Disodium hydrogen phosphate 0.55 g (0
,01~20.0g) Bromthymol Blue 0.14
g (0.05-0.5.9) purified water
1 l l l*NAD: Nicotine adenine dinucleotide 13, improved medium for urease reaction (URE) peptone for bacteria 1.0 (0.1-10.0 g) urea 3.0 (0.1-20.09
) Glucose 1.0 (0,01~5
．． 0 g) Sodium chloride 3.0 (0,01
~10g) Phosphoric acid-hydrogen dinato 0.03 (0,0
05-50.0g) Phenol red 0.19
(0.05-0.59) Purified water 1
The determination method for each medium is shown in Table 1.

■1 Specific actions and effects of the invention Using the microorganism identification plate of the invention described above,
To identify microorganisms, a test solution prepared by a conventional method is dispensed one drop at a time into each cylindrical body 12 using a dispensing dropper, and incubated at, for example, about 37° C. for 5 hours.

Each culture medium 20a to 20r fixed to each cylindrical body 12 in an annular manner
Since the amount is smaller than conventional ones, it dissolves easily and causes various reactions.

For example, according to the judgment table in Table 1, + or - is written on the matte surface 14 of the plate body 11 corresponding to each cylindrical body 12 depending on whether the reaction is positive or negative.

Microorganisms are identified by comprehensively judging the + and - indications.

Examples of experiments in which microorganisms were identified using the microorganism identification plate of the present invention will be described below.

Example A microorganism identification plate shown in FIGS. 1 and 2 was used.

Each of the above-mentioned improved media was used as each medium, and 50 μl of this was dispensed into each cylindrical body and dried.

Each enterobacterium shown in Table 2 was inoculated onto a trypto soy agar medium in a streak pattern and cultured at 37°C for 18 hours to form colonies.

Among the colonies in each plot, those with a diameter of 1.5 mm or more were suspended in 1 ml of sterile distilled water, dispensed drop by drop with a dropper for dispensing microorganisms, and incubated with a lid at 37°C for 5 hours. .

After culturing, GLC-NIT, H2S・IND and MAL
GLC for O-TDA respectively.

After judging H2S and MALO based on the color change based on the judgment table in Table 1 and writing + and - on the respective matte surfaces, for NIT, add the nitrate reduction reaction test reagent to IN.
For D, a reagent for indole production reaction test, and for TDA, a tryptophan deaminase reaction test reagent were added drop by drop.

and 0NPG, SOR, SAC, lNO, MAN.

One drop of the oxidase reaction test reagent was added to each negative reaction item among RAF and ADO.

The change in color after the addition of the reagent was observed, and a judgment was made based on the judgment table in Table 1, and + and - were written on the respective matte surfaces.

For other inspection items, changes in color were observed and + and - were written on the satin surface.

The results are shown in Table 3.

When the bacterial names were determined by comparing the combinations of + and - shown in Table 3 with the standard identification table, the same bacterial names as in Table 2 were obtained.

As described above, in the plate for identifying microorganisms of the present invention, the bottom surface of the cylindrical body constitutes a convex curved surface, and the culture medium is fixed to the circumferential part in a ring shape, so that the amount of culture medium can be reduced.

As a result, only a small amount of test liquid for identifying microorganisms is required, and microorganisms can be identified on the same day.

In addition, if the surface of the plate body corresponding to each cylindrical body is made of a matte finish, it is possible to write the + and - judgment symbols with a writing instrument, eliminating the need to memorize the judgment results and making it possible to accurately identify microorganisms. It can be done.

Furthermore, when the improved medium is used as a medium, each test reaction is further promoted and identification can be performed more accurately.

Furthermore, if a peripheral wall is formed in the plate main body so as to surround the cylindrical body, and a step is formed at the end of the peripheral wall, when the plates are stacked on top of each other, one of them functions as a lid and does not move. Convenient for transportation and storage.

[Brief explanation of the drawing]

FIG. 1 is a top plan view of the microorganism identification plate of the present invention;
FIG. 2 is a sectional view taken along the line ■-■ in FIG. 11... Plate body, 12... Cylindrical body, 13... Bottom surface, 14, 15, 16...
... Satin surface, 17 ... Peripheral wall, 18 ...
- Annular rib, 19... Annular protrusion, 20...
···Culture medium.

Claims (1)

[Scope of Claims] 1. A plate body, and a plate for accommodating a test liquid for identifying microorganisms, the plate body being spaced apart within the plate body so that the opening end is located on the same plane as the surface of the plate body. A microorganism identification plate comprising a plurality of bottomed cylindrical bodies each having a convexly curved bottom surface, and a microorganism identification medium annularly fixed to the periphery of the bottom surface of the cylindrical bodies. 2. Claim 1, characterized in that a cylindrical body is disposed externally, and the surface portion of the plate body at a position corresponding to the cylindrical body in the row direction is a surface on which symbols, etc. can be written.
Plate for identifying microorganisms as described in section. 3. The microorganism identification plate according to claim 2, characterized in that the cylindrical bodies are arranged in three rows. 4 The medium is Labremco Meat Extract Powder 0.01 to 0.0.
9g/13 water, bacterial peptone 0.1 to 9.0g/
l water, glucose 11.0 to 19. Of! /1! water, amminiram nitrate 0.1 to 5.0 g/l water, sodium bisulfite 0.01 to 5.0 g/l water, sodium bicarbonate 0.1 to 2 o, l/v water and phenol red 0.05 to 0 Medium consisting of a composition of .5 g/l water, Labremco meat extract powder 0.01 to 0.9
g/l water, bacterial peptone 0.1 to 9,09/13
Water, arabinose 11.0 to 19.0 g/l! water, sodium bisulfite 0.1 to 5.0971 water,
Medium consisting of sodium bicarbonate 0.1 to 2 o, og/l water and phenol red 0.05 to 0.5 g/l water, Labremco meat extract powder 0.01 to 0.9 g/l water, bacterial peptone 0 .1 to 9. (Jg
/11 water, rhamnose 11.0 to 19.0g/11
Water, sodium bisulfite 0.01 to 5.0 g/l
Water, sodium hydrogen carbonate 0.01 to 20.0 g/
l water and phenol red 0.05 to 0.5 g/
Medium consisting of l water composition, Labremco meat extract powder 0.0
1 to 1.09711 water, bacterial peptone 0.1 to 10.0 g/l water, disodium hydrogen phosphate 0.1
medium consisting of 1 to 30.0 g/l of water and 1 to 5% of sorbitol, saccharose, inosit, mannitol, raffinose or atonite, tryptone TO01 to 50.0 g/l of water, orthonitrophenyl-β-D gal') Topyranoside 0.5 to 5.0g
/l water, glucose O, 005g to 5.0g/IJ
Water, sodium bisulfite 0.01g to 0.5g/
l water and 0.1 to 2 o of disodium hydrogen phosphate
, medium consisting of og/l water composition, liver extract powder 0.0
1 g/l water or more, yeast extract powder 0.01 g/l water or more, tryptone 0.01 g/lj water or more, proteose peptone 0.01 g/l water or more, soya peptone 0.0
19/l water or more, sodium thiosulfate 0.19 to 5
．． 0 g/l water, iron ammonium citrate 0.1 g to 5.09 g/l water, L-tryptophan 0.01 g/l or more and dipotassium phosphate 0.1 to 20.0 g/l water.
Medium consisting of 1 water composition, protease peptone 0.1
from 20.0 g/l water, sodium malonate 5.0 to 30.1/l water, I,4-Liptophan 0.01 to 10.1/l water, glucose 0.01 to 5.0 &
/l water, sodium chloride 0 to 5.0 &/l water, sodium dihydrogen phosphate 0.1 to 2o, og/13 water and bromothymol blue 0.05 to 0.5 g/l
Medium consisting of 13 water composition, bacterial peptone 0.1 to 6.0 &/l water, Sawyer peptone 0.1 to 4.0
&/l water, yeast extract powder 0.1 to 5.0 g/13 water, glucose 0.1 to 30.0 &/l water, sodium pyruvate 0.1 to 15.0 g/# water, sodium bisulfite 0. 01 to 5.09711 Water, sodium dihydrogen phosphate 0.01 to 10.0 p/l and creatine 0.01 to 5.0 &/l water, tryptone TO, 01 to 20.0 &/l
Water, L-lysine-hydrochloride 5.0 to 5o, og/l water, glucose 0.01p to 5.0g/IJ water, sodium dihydrogen phosphate 0.01 to 2o, og, #water and bromothymol Blue 0.05 to 0.5g/
A medium consisting of a composition of 12 water, tryptone TO, 01 to 20.0 &/l water, L-ornithine hydrochloride 5.0 to 50.0 &/l water, glucose o, oB to 5.
09/l water, sodium dihydrogen phosphate 0.01 to 20.09/l water and bromothymol blue 0.05
A medium with a composition of 0.59 to 0.59/l water, 0.
．． 01 to 30.0 g/l water, L-arginine hydrochloride 5.0 to 50.0 g/l water, glucose 0.
0.01 to 5.0&/l water, sodium dihydrogen phosphate 0.01 to 20.0 g/l water and bromothymol blue 0.05 to 0.5 g/l! A medium consisting of a water composition, yeast extract powder 0.01 to 3.09/l water,
Sodium citrate 0.5-20, o9/l water, glucose 0.005-0.1 g/It water, reduced glutathione 0.01-10.097! 'Water, nicotine adenine dinucleotide 0.005 to 5.0 &/
l water, sodium chloride 0.5 to to, og/l water,
Phosphate-hydrogen disodium 0.01 to 20.0&/
l water and bromothymol blue 0.05 to 0.5
A medium consisting of the composition of &/l water and peptone 0 for bacteria
．． 1 to 10.0 g/# water, urea 0.1 to 20.
0g/11 water, glucose 0.01 to 5.0g/1
3 water, sodium chloride 0.01 to 1o, og/# water, phosphoric acid-hydrogen disodium 0.005 to 50.0
g/l! water and phenol red 0.05 to 0.05 o;
The microorganism identification plate according to any one of claims 1 to 3, which is selected from the group consisting of a medium having a composition of l/ll water. 5. Claim 1, characterized in that a peripheral wall is formed that is continuous with the peripheral end of the plate body and surrounds the cylindrical body, and an annular rib is provided that projects laterally outward from the lower end of the peripheral wall.
The plate for identifying microorganisms according to any one of Items 1 to 4.