JPS63202357A - Health food - Google Patents

Abstract

PURPOSE:To obtain a health food containing Solanum lyratum Thumb as an active ingredient and having antitumor action without toxicity. CONSTITUTION:A leaf, steam or the both or whole grass of Solanum lyratum Thumb which is a climbing and perennial plant of Solanaceae autogenous in Japan, India, China continent, etc., is as necessary (semi-)dried and extracted with water or a water-soluble organic solvent (e.g. ethanol) at room temperature or under heating to provide an extract, which is then filtered and dried to afford the extract of Solanum lyratum Thumb. Then the extract is taken in an amount of 1g/adult/day.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to Solanum Iyra
The present invention relates to a health food containing an extract of tumThunb (Japanese and English, white haired wisteria) as an active ingredient.

[Prior art and its problems (background of the invention)] Hyodorino 1
Ugo is a climbing perennial herb of the Solanaceae family that grows wild in the mountains and roadsides of Japan, India, mainland China, etc., and has traditionally been used as a folk medicine for fever reduction, diuresis, tonicity, stomach health, and pain relief. However, it was not used much because it was said to be toxic, and its active ingredients, efficacy, toxicity, etc. had not yet been fully investigated. In view of this current situation, the present inventors conducted a detailed study on the physiological effects of the components contained in Bulbul vulgaris, and found that the extract of Bulbul vulgaris with water etc. has antitumor effects. The present inventors discovered that the present invention was based on the discovery that a health food with excellent efficacy could be obtained by using the compound, and that it was not particularly toxic.

[Structure of the invention]

The gist of the present invention, which was made against the background of such circumstances, is that the present invention uses a red-spotted bulb extract as an active ingredient,
JI7 for 0 wood is composed of a health food with anti-tumor effects! In I, the term "Hyodorino vulgare extract" refers to an extract obtained by extracting the Hiyodorino genus with water, a water-soluble organic solvent, or a mixture of water and a water-soluble organic solvent.

The extract used as an active ingredient in the present invention can be prepared by extracting fresh, dried or semi-dried leaves, stems, foliage, and whole plants of Hyodorino 1. M), ethanol, and other alcohols (M) are likely to be extracted by leaching with a mixture of these at room temperature or by heating. Obtained by drying using a drying method.

Although it is possible to use known water-soluble organic solvents, it is preferable to use one that does not adversely affect health.

The health food of the present invention may be used as is,
In addition, according to a conventional method, known excipients for food and other compounds are mixed at a rate such that the intake amount of the extract is about 1 g per adult per day, and it is prepared in the form of a normal food.
For example, powder, 12M.

It can also be in the form of capsules, granules, suspensions, syrups, and other solid, semisolid or liquid conventional food forms. Fl! There are no particular limitations on the ingredients, and it is also possible to add Hyodorinorougo extract to other foods at the above-mentioned ratios.

〔Effect of the invention〕

According to the present invention, foods having antitumor effects can be ingested as food, which greatly contributes to maintaining health.

In any case, it is not yet known that the health food of the present invention, which contains the extract of the present invention as an active ingredient, not only has an antitumor effect, but also has no particular toxicity. It can be said that f and s are extremely large.

[Examples] - The present invention will be further explained below with reference to Examples and '171 Experimental Example, but the present invention is not limited thereto.

χ side 1 16,001 liters of water in dried calamus leaves so*g
and extracted with an extract extractor at 100℃ for 1 hour.
After filtering the insoluble matter, the obtained extract was concentrated under reduced pressure at 40° C. and further spray-dried to obtain yellowish brown dry extract powder 4Ay. The yield was 6.7%.

Example 2 Total jF of dried bulbul, 45kg, 10
Extract with hot water at 0°C for 1 hour, filter the insoluble matter, and add 4
A soft extract (fluid extract) 381 with a concentration of 12% was obtained by vacuum low-temperature concentration at 0°C. The dry extract powder amount per extract 11e was 0.28 g/xi.

Example 3 (Tablet M) Dry extract 2 produced in Example 1 above was mixed with 9 and lactose 2, and a small amount of flavoring agent was added, and the mixture was compressed into tablets each weighing 1 g. This tablet should be taken twice a day.

Next, the results of testing the efficacy of the f11 health food of the present invention are shown.

Test Example 1 Sarcoma 180 (M, tumor cells) using mice
(Preparation of sample) 700 g of the air-dried stems of B. elegans was extracted with hot water at 60°C, and the extract was divided into a water layer and a butanol (BuOH) layer by partitioning the amount of solvent between water and lobe tar. fractionated into
Furthermore, the fraction of the aqueous layer was extracted using berlite XAD4 (
The mixture was fractionated into a methanol (NeOH) eluate and an aqueous eluate by column chromatography using (trade name).

(Sample administration and effect) Using the WiA solution obtained in the above fractionation, Sarcoma 1
The activity against 80 was investigated. In other words, 5areo theory a
180 was transplanted into the mouse skin, the aqueous layer,
Both the BuOH layer, the aqueous eluate, and the MeOH eluate were each administered once a day at a rate of 1F body weight to 2000.3000.3000.1100Oz/10 times in total.
&11 As shown in Table ■, the proliferation was suppressed in all cases compared to the control group (non-administered group). In particular, the BuOH layer showed the strongest suppressive effect when the tumor weight was about 173 compared to the control group.

Table 1 Table ■ Test Example 2 Suppressive effect on JTC-26 (entered cervical cancer cells) using mice (preparation of sample) Bulbul bulbulina was extracted as shown in Figure 1, and the sample was Obtained.

That is, 1.1 A2 of the air-dried stems of the water-drying plant were extracted three times with warm water at 60°C, and the extract was concentrated under reduced pressure at 60°C or lower to obtain 133 g of Suikokisu. After treating this with ethyl acetate (^cOEL) 400z1, the remaining fi12
4f was suspended in water 31 and extracted by shaking with BuOH 31.

On the other hand, the stems after hot water extraction were extracted twice with hot MeOHr and twice with heating, and the extract was concentrated under reduced pressure to obtain 38 g of HeOH extract.

In addition, when we searched each of Suiko Kiss, ^eOEt soluble part, BuOH layer, aqueous layer, and HeOH extract by thin layer chromatography, the chromatogram shown in Figure 2 was obtained, and ^coEtc+1 part contained ll1lF fat, etc. but,
It was found that sugar was mainly transferred to the aqueous layer.

The BuOH layer was then concentrated under reduced pressure to obtain a residue stg, and as shown in FIG.
Repeat column chromatography using (trade name), silica sol column chromatography, recrystallization, etc.
Steroid saponin 5L-0 (2), 5L-1 (1),
7.9.1.6 5L-11(3) respectively as crystals
．． 3.4g was obtained. The yield for dry raw materials is 0 for each
．． It was 72.0°15.0.31%. Also, HeO
From the H extract, SL-1 (3.9 g (0.36% based on the dry raw material) was obtained by repeating silica sol column chromatography.

(Sample administration and effects) Steroid saponin 5L-0 (2) obtained by the method of Moeji,
5L-1 (1), SL-■ (3) JTC-26
We investigated its effect on cervical cancer. J
HEM TC-26 to 1 x 105 cells/ml
Eagles 90%, Feta-ICalf Ser
um (Product name: Microbio-1 logica 1 company 1
31) The cells were placed in a 10% medium, and the compound was further adjusted to each concentration and injected. Five samples were prepared for each sample, and samples with no additives were used as a control group.The samples were incubated at 37°C for 144 hours in CO□x flow, and the number of viable cells was counted.
The growth inhibition rate was measured by comparing the mean value with the control group using AIL. The results are shown in Table ■.From this result, 5L-1(
1) and SL-■ (3) were found to have a strong inhibitory effect. SL-■ (3) shows no activity at low concentrations, but when the concentration becomes lighter than 8.0 gg/11, the activity increases rapidly, showing an inhibition rate of 100%. -#s70
No activity was observed with star 7-luglycoside 5L-0(2).

&l
The following acute 4-sex test using mice etc. and ■E2
A sexual toxicity test was conducted.

Acute toxicity test was conducted in mice (mouth^L8/ce^nw Cr
In j), the bulbul extract obtained in Example 1.2 was administered orally or intraperitoneally.

In the case of oral administration, the brown-spotted bulbul extract obtained in Example 2 was administered to a group of 10 8-week-old male mice at a dose of 5.6 kg.
y/kg body nails and 11.297 kg body weight. LDS after 72 hours. is 11,2y#g
That was it. In the case of intraperitoneal administration, the Hyodorino 1 Ugo extract obtained in Example 1 was used at an extract concentration of 0.38 g/Ay.
.

0.95g/kFI, 1.9g/A1F, 3.81F,
Physiological saline was used to maintain the Qg of 7.8 g/average, and the amount of solution administered to a group of 1044-week-old male mice was L.
Ozl/kg. LD after 72 hours was 2.05y/AI.

LD of the hot water soluble fraction of dried leaves of Kumazasa. is more than 9 in 10g7 for oral administration, and 2.2097kg (1 for intra-abdominal administration).
975 pm Dr. Yanada et al. of Pharmaceutical University published in Japanese Pharmacological Journal:
(Pharmacological research on bear bamboo), oral administration of Hycidorino 1 Ugo extract, LD of intra-abdominal administration. The level of toxicity was similar to this one, and no particular toxicity was observed.

Subacute toxicity test was conducted on 4-week-old and 8-week-old male and female mice (B
AL8/c, ^nwcrj), 6-week-old female rats (Cr
Lllister) and 4-week-old female guinea pigs (Cr
In the case of administration for 8 weeks, the internal intake per 14 body weight was approximately 38 y/Ag for mice, approximately 12.2 g for rats, and 14 y/Ag for guinea pigs. .8y/Ag, when administered for 13 weeks, it was 55g/Ay for mice and 16,71g for rats.
7A9, guinea pig was 24.4 g/approx.

According to this test, no significant changes due to the difference in period were observed in the observation at 8 weeks 1 and 11.13 weeks (111 weeks), - the membrane condition was normal, and both body weight change and food deterioration were significant compared to the control group. No differences were observed.In addition, no changes were observed due to gender differences in pathological Jfl literacy findings, protein levels λ, etc., and no significant differences were observed in blood cell counts, etc. compared to the control group.Furthermore, major organs, etc. The pathological W;L examination was normal in both mice, rats, and guinea pigs, and no remarkable 5% normal state was observed overall.

From the above tests, it is clear that the red-spotted bulbul extract does not have any significant rQ properties.

Incidentally, in each of the above-mentioned Examples and the like, dried sagebrush is used, but it is also possible to use fresh or semi-dried sagebrush.

[Brief explanation of the drawing]

Figure 1 is a diagram of the fractionation process of the test sample of the present invention;
The figure is a thin) II chromatogram of P. elegans extract.

Claims (1)

[Claims] A health food containing bulbul extract as an active ingredient and having antitumor effects.