JPS632011Y2 - - Google Patents

Description

[Detailed explanation of the invention] This invention relates to a device for measuring chemiluminescence.
In particular, the present invention relates to a device for measuring weak light emitted from living organisms such as laboratory animals.

As is well known, many substances generate extremely weak light as a result of oxidation reactions, etc., and the applicant has put into practical use a device that detects and quantitatively measures this weak light. There is.

Figures 1 and 2 show the device.
11 is a casing from which light is blocked, and this casing 11
A drawer-shaped sample chamber 12 is provided at the lower part of the front surface. A sample storage cell 13 shown in FIG. 2 is provided inside this sample chamber 12, and this cell 13
is set at a predetermined position in the housing 11 by pushing the sample chamber 12 into the housing 11. Further, a photoelectric converter (for example, a photomultiplier tube) 14 is provided inside the casing 11 facing the cell 13, and a shutter 15 is provided at an intermediate portion between the photoelectric converter 14 and the cell 13. . When the shutter 15 is open, weak light generated from the sample housed in the cell 13 is detected by the photoelectric converter 14. The output signal of this photoelectric converter 14 is supplied to a counting section 17 via a preamplifier 16, and the input signal in this counting section 17 is, for example, A/D (analog/digital) converted and then counted. The amount of weak light emitted is measured.

Incidentally, chemiluminescence has been confirmed not only in substances such as oil but also in blood and the like in living tissues. Conventionally, when measuring the amount of light emitted from blood or the like, blood is collected from a living body and stored in the cell 13. Therefore, it has been difficult to carry out experiments in which, for example, changes in the amount of light emitted when a reagent is administered to a living body are measured in real time.

In the fields of basic medicine and clinical medicine, it is important to use experimental animals and measure chemiluminescence in the brain, liver, etc. in living animals. However, since conventional devices use cells, it has been difficult to measure living organisms.

This idea was made based on the above circumstances, and its purpose is to be able to directly measure chemiluminescence from a living body, to move the living body to a predetermined position relative to the photoelectric converter, and to expand the measurement range. By making it possible to set it arbitrarily, the present invention aims to provide a weak light measuring device that has few measurement errors and is highly practical.

An embodiment of this invention will be described below with reference to the drawings. Note that the same parts as in FIG. 2 are given the same reference numerals, and only the different parts will be explained.

In FIG. 3, a container, for example a vat 31, is provided inside the sample chamber 12, and this vat 31 is provided inside the sample chamber 12.
A living body 32 such as a rat is housed inside the body 1 . In addition, the sample chamber 1 located below the vat 31
A drive device 33 is provided inside the butt 2, and a drive shaft 34 of this drive device 33 is provided on the lower surface of the butt 31. This drive device 33 drives a drive shaft 34 manually or by power, and the butt 31 is driven in the vertical direction, that is, in the direction of distance from the photoelectric converter 14. Further, a mask 35 is provided between the living body 32 and the shutter 15 in correspondence with the light receiving section of the photoelectric converter 14. This mask 35 is fixed to, for example, the sample chamber 12 by supports 36 and 37, and a diaphragm 41 is provided in the center of the mask 35 as shown in FIG.
is detachably provided. The mask 35 and the aperture 41 define the measurement position and measurement range of the living body 32, and various apertures 41 with different diameters of the through holes 42 are prepared. In such a configuration, the living body 32 is placed inside the vat 31 so that the measuring section faces the through hole 42 of the diaphragm 41 . At the same time, the drive device 33 is operated, and the butt 31 is moved up and down to connect the living body 32 and the mask 3.
5 and the photoelectric converter 14 are adjusted.

According to the above configuration, the bat 31 is connected to the drive device 33.
It can be driven in the vertical direction by. Therefore, by driving the butt 31, even if the living body has a complicated shape, the living body and the photoelectric conversion device 14 can be separated.
It is possible to keep the distance constant, and it is possible to reduce detection errors in the amount of light emitted due to distance errors.

Furthermore, by providing the mask 35, it is possible to accurately determine the part of the living body to be measured.
Further, by appropriately changing the diameter of the through hole 42 in the diaphragm 41, the amount of light and the measurement range can be changed, so there is an advantage that the resolution can be adjusted as appropriate.

Next, another embodiment of the present invention will be described. In Fig. 5, the same parts as in Fig. 3 are given the same reference numerals, and only the different parts will be described.

In FIG. 5, a butt 51 is provided on the drive shaft 34 of the drive device 33, and a smaller butt 52 is provided inside this butt 51. Moving devices 53 and 54 are provided between the butts 51 and 52 to connect them and drive the butt 52 in the front, back, left and right directions inside the butt 51. The moving devices 53 and 54 each include, for example, a gear and a nut, and the butt 52 can be moved to an arbitrary position by, for example, driving the gear manually or by power.

With the above configuration, the vat 51 can be driven in the vertical direction and in the front, rear, left and right directions while the living body 32 is housed inside the vat 51. Therefore, the positional relationship between the measurement site of the living body and the photoelectric conversion device 14 can be easily set.

Furthermore, if a sensor is provided to detect the amount of movement of the bat 52 and the bat 52 is moved back and forth or left and right while the vertical position of the living body is set, it is possible to measure spatial changes in the amount of light emitted by the living body. It is.

In FIG. 5, reference numeral 55 is a plug provided on the housing 11, and a pipe for supplying anesthetics or the like to the living body is inserted through the plug 55.

Further, the configurations of the diaphragm 41 and the moving devices 53, 54 are not limited to the above embodiments, and can of course be modified in various ways.

As detailed above, according to this invention, chemiluminescence from a living body can be directly measured, the living body can be moved to a predetermined position relative to the photoelectric converter, and the measurement range can be set arbitrarily. By this,
It is possible to provide a highly practical weak light measurement device with little measurement error.

[Brief explanation of the drawing]

Figures 1 and 2 show conventional weak light measuring devices, respectively. Figure 1 is a front view, Figure 2 is a configuration diagram, and Figure 3 is an example of a weak light measuring device according to this invention. FIG. 4 is a top view showing a part of FIG. 3, and FIG. 5 is a block diagram showing another embodiment of this invention. 11... Housing, 14... Photoelectric converter, 31, 5
1, 52... Butt, 32... Living body, 33... Drive device, 35... Mask, 41... Aperture, 53,
54...Moving device.

Claims (1)

[Scope of utility model registration request] In a weak light measurement device that uses a photoelectric converter to detect chemiluminescence generated from a biological sample inside a light-blocked housing and measures the amount of light emitted from the detected light, a container for accommodating a biological sample with an opening facing the photoelectric converter; and a container capable of driving the container to maintain at least a constant distance between the biological sample contained in the container and the photoelectric converter. A weak light measuring device comprising: a driving means; and a setting means provided between the biological sample and the photoelectric converter to determine the measurement range of the biological sample and to set the amount of light incident on the photoelectric converter.