JPS63159498A - Enzyme-containing tableware washing composition - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides an exa protein containing a protease and a specific enzyme.
□Washing, acid (enzyJic dishwashin)
qComposition).

The use of II in hand and machine dishwashing compositions is known in the art. For this purpose, amylases and/or proteases have been proposed in particular.

Although lipases have also been suggested as general enzymes, no specific lipases have been proposed for use in dishwashing compositions.

A frequent problem in dishwashing is the removal of egg yolks, especially completely dried or baked hardened egg yolks, from the surfaces of dishes, and the satisfactory removal of this type of soil often requires specific measures or specific dishwashing methods. Requires composition.

The inventors have surprisingly found that the inclusion of proteases and certain lipases in dishwashing compositions can satisfactorily remove egg yolks from dishes.

This removal effect is significantly superior to that obtained using either protease or lipase, and in fact a synergistic cleaning effect can be obtained by combining these two enzymes.

Proteases that can be used in the present invention are any type of protease known for inclusion in detergent compositions. A widely commercially available protease is also the N0VOI/species, and suitable proteases of this type include N0VOI
Alcalase commercially available from ndustri.

IEsperase and 5avinasc; G15
HaXataSe and H'axacal commercially available from Broca-des; Kali Chew+ie
Optimase, commercially available from Showaden Riki; azusase, commercially available from Showaden Riki. Preferably, it is a so-called highly alkaline protease, such as 5avinase. Mixtures of various proteases may also be used. usually,
The dishwashing composition of the present invention contains protease 9, and the final composition has a suspension of 0.1 to 50 GU/N, usually 1 to 40 G.
U/tttg, preferably with a proteolytic activity of 5-30 GU/q.

The lipase that can be used in the present invention is derived from the microorganism Chromo
bacter viscosum var decoy stage"N
This is a lipase that shows positive immunological cross-reactivity with the lipase antibody produced by RRL-B56731. The lipase is manufactured by Ministry of Agriculture Re5earc.
h5service, NorthernLltili
zation and Development Di
available as NrlRL-83673. This lipase is hereinafter referred to as "Toyo 1iffiJ lipase".
n,, 133. p., 76-79 (1950)], it must exhibit positive immunological cross-reactivity with the Toyo Jozo lipase antibody.

Antiserum was produced as follows.

0.1 rI of equal volume ■! Mix the g/d antigen and Freund's adjuvant (complete or incomplete) until an emulsicon is obtained. Two female rabbits are injected with emulsion 2d in the following manner.

Day 0: Antigen in complete Freund's adjuvant Day 4: Antigen in complete Freund's adjuvant Day 32: Antigen in incomplete Freund's adjuvant Day 60: Booster of antigen in incomplete Freund's adjuvant Clots collected on day 67 were centrifuged. Serum containing the required antibodies was prepared.

The titer of the anti-Toyo III lipase antiserum is determined by observing the precipitation of serial dilutions of antigen and antiserum according to the Ophterlony method. 25 dilution of antiserum is 0.119/
at! The dilution was such that visible precipitation was observed at an antigen concentration of .

All lipases that exhibit positive immunological cross-reactivity with the Toyo Inn Lipapi antibody described above are included in the lipases of the present invention. As a typical lipase, Pseu-domonas
Lipase derived from Nuorescens TAN 1057 (trade name Amano-P), Pseudomon
as rrragi FERN P1339-derived lipase (trade name Amano-B), Pseu-domo
nas n1troreducens var, lip
lipase derived from P. olyticum FERN P1338, lipase derived from PSOUdOmOnaS Sp,
Product name Amano-CES), Pseudomon
Lipase from as2 dish, ChrOmObaCtOr
For example, Chromobacter v
iscosum var, Lipolyt-icun
Lipase derived from NRRL B-3673 (commercially available from Toyo Jozo), US Biochemical Ca.
rp, and Chromobacter viscosus lipase commercially available from DiO3ynth Co, Netherlands, and lipase from Pseudomonas ladioli.

The lipase of the present invention in the dishwashing composition is preferably such that the final dishwashing composition has a concentration of 100 to 0.005 LU/j19, preferably t L
, < 25 to 0.05 LU/19 with lipolytic activity.

Lipase unit (tu), temperature 30℃, al19.0 (
7) Under conditions 5 mmol/Il of Tris! 1 in buffer solution
3 mmol/1 Ca24 and 20 mmol/1 Ha
It is the amount of lipase that produces 1 μmol of titratable fatty acids per minute in the presence of α. The substrate is 3.3% a%
of olive oil and 3.3% of gum arabic.

Of course, it is also possible to use mixtures of the abovementioned lipases. The lipase may be used in an unpurified state or in a purified state purified by a known adsorption method such as phenylcephalic acid adsorption method.

The dishwashing compositions of the present invention may further contain ingredients conventional in dishwashing compositions. Thus, the dishwashing compositions of the invention may contain small amounts, for example 0.5 to 5% by weight, of detergent surfactants such as anionic, nonionic surfactants, such as low-foaming or non-foaming nonionic surfactants. The low-foaming or non-foaming nonionic surfactants are known in the art, and suitable surfactants are described in "Non1o" by H.
nic 5urfactants"Vol, 1 (19
67).

Dishwashing compositions of the present invention may also contain organic and/or inorganic builder substances, usually from 10 to 80% by weight, and for most common applications from 20 to 60% by weight. The builder substances include polyphosphoric acid, birophosphoric acid, hexametaphosphoric acid, orthophosphoric acid, carbonic acid, bicarbonic acid, and boric acid.

Also included are alkali metal salts of silicic acids and alkali gold salts of polycarboxylic acids and poly- and hydroxysulfonic acids.

Further examples include citric acid, nitrilotriacetic acid, carboxymethyloxysuccinic metal salts, and zeolites. Polyelectrolytes such as polymaleates and polyacrylates are also suitable.

Perboric acid. Hypercarbonic acid. It is also possible to include peroxy type bleaches such as alkali gold Hg persulfates and organic peracids and their salts. A bleaching precursor such as tetraacetylethylene diamine may be included along with a peroxy type bleach such as sodium perborate.

Depending on your needs! ! ! Bamboo liquid, fragrance, @coloring agent, disinfectant.

Solvent, antifoaming agent. Re like hectorite.

Corrosion inhibitor. It is also possible to contain a discoloration inhibitor and the like.

It is also possible to contain other enzymes, such as amylases, cellulases, pectinases, pectin-esterases or oxidases.

The composition can be prepared in any desired form, eg powder, bar. cake.

It can be prepared in block, paste and liquid form.

Examples of the present invention will be shown below.

Example 1 The following basic ingredients Pentasodium triphosphate 1.16 g/l.

Anhydrous sodium carbonate 0.27 9/1 Sodium sulfate 0.33 9/1 and anhydrous sodium sulfate 0.5619/j! The test was conducted using an aqueous solution containing .

Toyo Jozo lipase (0.259/1) is added to the above composition.
), Savinase 6. OCM (0.02
Comparative tests were conducted on products containing 3g/I) or a mixture of these enzymes, or those containing no enzyme.

A set of 8 glass plates (4x4c!I) was coated with baked-o
n) Egg yolk attached. These were soaked in water 11 at 27° G11 containing the above amount of dishwashing composition for 1 hour at pl+10.0. The amount of residual egg yolk present on the board was measured by weighing the soiled board before and after soaking and calculating the difference in weight.

The difference (DW) was expressed as a percentage of the initial amount of soil. The test was repeated three times and the following results were obtained.

Similar results were obtained at 35°C and 45°C.

Similar results were obtained when Alcalase was used in place of 5avinase.

Example 2.

The following results were obtained in the same manner as in Example 1 except that Amano-P lipase was used.

Example 3 Glassware was washed and hot dried in a Kervore 5ears dishwasher at 60° C. according to a normal washing program b4:. The hardness of water is 14.4°
It was FH.

Dishwashing composition 309 having the following composition was charged.

Sodium tripolyphosphate 24wt 8% soda ash
20 Sodium disilicate 11% sodium disilicate Condensate of linear C1o alcohol with 6 moles of ethylene oxide and 24 moles of 2.5% propylene oxide Sodium sulfate
27.8% by weight Amylase (4,8H
U/Ill! J) 0.5ffiffi%・
■ Protease (Sav+nase) 1.0
Weight % (1544GII/~) Toyo Jozo Lipase 15LU/d stain is 6
It was the egg yolk of Itg.

The glassware was washed several times in succession and evaluated for degree of film formation on a scale of 0 to 5. It was determined as 0 when no clouding occurred at all, and 5 when severe clouding occurred. These experiments were also conducted using compositions containing no lipase, compositions containing no 5avinasc, or compositions containing neither lipase nor 5avinase.

I got the following results.

-”'J- Par Namrose Pen Note Sharp

Claims (2)

[Claims] (1) 0.5-5% by weight detergent surfactant and 10-80%
An enzyme-containing dishwashing composition comprising % by weight of builder and an amount of protease such that the composition has a proteolytic activity of 0.1 to 50 glycine units/mg, further comprising:
romobacter viscosum￥var、￥
1. A composition comprising 0.005 to 100 LU/mg of bacterial lipase that exhibits a positive immunological cross-reactivity with an antibody to lipase produced by C. lipolyticum\NRRL B3673. (2) Lipase is Pseudomonas fluo
rescens￥、￥Pseudomonas fra
gi￥、￥Pseudomonas nitrored
ucens\var,\lipolyticum\,\
Pseudomonas cepacia￥、￥Pse
u-￥￥domonas gladioli￥、￥Ch
The composition according to claim 1, which can be produced from Romobacter viscosum.