JPS63152998A - 糖転移酵素の測定方法 - Google Patents
糖転移酵素の測定方法Info
- Publication number
- JPS63152998A JPS63152998A JP29933386A JP29933386A JPS63152998A JP S63152998 A JPS63152998 A JP S63152998A JP 29933386 A JP29933386 A JP 29933386A JP 29933386 A JP29933386 A JP 29933386A JP S63152998 A JPS63152998 A JP S63152998A
- Authority
- JP
- Japan
- Prior art keywords
- sugar
- transfer
- acceptor
- transferase
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000000346 sugar Nutrition 0.000 title claims abstract description 63
- 102000004357 Transferases Human genes 0.000 title abstract description 13
- 108090000992 Transferases Proteins 0.000 title abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 23
- 239000002773 nucleotide Substances 0.000 claims abstract description 18
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 18
- 239000000126 substance Substances 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 3
- 108700023372 Glycosyltransferases Proteins 0.000 claims description 20
- 102000051366 Glycosyltransferases Human genes 0.000 claims description 19
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 claims description 12
- 239000000470 constituent Substances 0.000 claims 1
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 10
- 239000011324 bead Substances 0.000 abstract description 8
- 238000012546 transfer Methods 0.000 abstract description 7
- 229930186217 Glycolipid Natural products 0.000 abstract description 6
- 102000004856 Lectins Human genes 0.000 abstract description 6
- 108090001090 Lectins Proteins 0.000 abstract description 6
- 239000002523 lectin Substances 0.000 abstract description 6
- 239000007853 buffer solution Substances 0.000 abstract description 4
- 150000002772 monosaccharides Chemical class 0.000 abstract description 3
- 229920001542 oligosaccharide Polymers 0.000 abstract description 3
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 3
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 abstract description 2
- 230000004913 activation Effects 0.000 abstract description 2
- 239000000439 tumor marker Substances 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 150000001455 metallic ions Chemical class 0.000 abstract 1
- 230000005855 radiation Effects 0.000 abstract 1
- 239000000370 acceptor Substances 0.000 description 14
- 102000003886 Glycoproteins Human genes 0.000 description 8
- 108090000288 Glycoproteins Proteins 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 150000008163 sugars Chemical class 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- OGGXGZAMXPVRFZ-UHFFFAOYSA-N dimethylarsinic acid Chemical compound C[As](C)(O)=O OGGXGZAMXPVRFZ-UHFFFAOYSA-N 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 239000012190 activator Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000000691 measurement method Methods 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 102000030902 Galactosyltransferase Human genes 0.000 description 2
- 108060003306 Galactosyltransferase Proteins 0.000 description 2
- 108010044467 Isoenzymes Proteins 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229950004243 cacodylic acid Drugs 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229930182830 galactose Natural products 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- WTOIRKBUWMPVHG-VEIQOZLZSA-N (3R,4R,5S,6R)-3-amino-2-hexadecyl-6-(hydroxymethyl)oxane-2,4,5-triol Chemical compound CCCCCCCCCCCCCCCCC1(O)O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1N WTOIRKBUWMPVHG-VEIQOZLZSA-N 0.000 description 1
- HYZAWROTMYAUAQ-UHFFFAOYSA-N 1-ethyl-3-(6-methylpyridin-2-yl)urea Chemical compound CCNC(=O)NC1=CC=CC(C)=N1 HYZAWROTMYAUAQ-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000009016 Cholera Toxin Human genes 0.000 description 1
- 108010049048 Cholera Toxin Proteins 0.000 description 1
- PNIWLNAGKUGXDO-UHFFFAOYSA-N Lactosamine Natural products OC1C(N)C(O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 PNIWLNAGKUGXDO-UHFFFAOYSA-N 0.000 description 1
- -1 N-acetyl glycosamine Chemical class 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000244317 Tillandsia usneoides Species 0.000 description 1
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 description 1
- USAZACJQJDHAJH-KDEXOMDGSA-N [[(2r,3s,4r,5s)-5-(2,4-dioxo-1h-pyrimidin-6-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] hydrogen phosphate Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](C=2NC(=O)NC(=O)C=2)O1 USAZACJQJDHAJH-KDEXOMDGSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000007978 cacodylate buffer Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
- 230000000503 lectinlike effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 229960001479 tosylchloramide sodium Drugs 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29933386A JPS63152998A (ja) | 1986-12-16 | 1986-12-16 | 糖転移酵素の測定方法 |
| EP87118665A EP0272603A3 (en) | 1986-12-16 | 1987-12-16 | Method for measuring glycosyltransferase |
| US07/643,695 US5173407A (en) | 1986-12-16 | 1991-01-22 | Method for measuring glycosyltransferase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29933386A JPS63152998A (ja) | 1986-12-16 | 1986-12-16 | 糖転移酵素の測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63152998A true JPS63152998A (ja) | 1988-06-25 |
| JPH0548120B2 JPH0548120B2 (https=) | 1993-07-20 |
Family
ID=17871193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29933386A Granted JPS63152998A (ja) | 1986-12-16 | 1986-12-16 | 糖転移酵素の測定方法 |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP0272603A3 (https=) |
| JP (1) | JPS63152998A (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315761A (ja) * | 1989-03-15 | 1991-01-24 | Sumitomo Seika Chem Co Ltd | 糖転移酵素の活性測定方法およびその用途 |
| JPH03140865A (ja) * | 1989-10-27 | 1991-06-14 | Kosumitsuku:Kk | 悪性腫瘍の判別方法 |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4971905A (en) * | 1987-08-11 | 1990-11-20 | Pacific Northwest Research Foundation | Diagnosis of cancerous or precancerous conditions in human secretory epithelia by enzyme activity of β-1-3N-acetylglucosaminyltransferase |
| US5403717A (en) * | 1987-08-11 | 1995-04-04 | Pacific Northwest Research | Diagnosis of premalignant or malignant conditions of human secretory epithelia |
| JP2579497B2 (ja) * | 1987-09-07 | 1997-02-05 | オリエンタル酵母工業株式会社 | 肝臓疾患診断剤 |
| DK626488D0 (da) * | 1988-11-10 | 1988-11-10 | Torben Falck Oerntoft | Fremgangsmaade til udtagning af en sekretproeve via en legemsaabning og til praeparation af proeven med henblik paa kvantitativ bestemmelse af molekylaere strukturer og enzymaktivitet i proeven samt proeveudtagningsindretning til brug derved |
| US5206356A (en) * | 1988-11-21 | 1993-04-27 | Chembiomed, Ltd. | Inhibitors for alpha-L-fucosyl transferase |
| US5032505A (en) * | 1988-11-21 | 1991-07-16 | Chembiomed, Ltd. | Inhibitors for glycosaminosyl transferase V |
| CA2012062A1 (en) * | 1989-03-15 | 1990-09-15 | Shin Yazawa | Method for determination of activities of glycosyltransferases and its use |
| CA2147887A1 (en) * | 1992-10-30 | 1994-05-11 | James W. Dennis | Method for measuring glycosyltransferase activity |
| WO2006049519A1 (en) * | 2004-11-08 | 2006-05-11 | Meat Biologics Research Limited | A method for the determination of sialyltransferase activity |
| US20240167075A1 (en) * | 2022-11-17 | 2024-05-23 | Regeneron Pharmaceuticals, Inc. | Methods for assaying enzyme activity |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2621908A1 (de) * | 1975-05-26 | 1976-12-09 | Sandoz Ag | Neues diagnostikum fuer maligne tumore |
| DE3070194D1 (en) * | 1979-04-16 | 1985-03-28 | Massachusetts Inst Technology | An in vitro method for determining malignancy in mammalian tissue |
| NL8020265A (nl) * | 1980-07-25 | 1982-05-03 | Otsuka Pharma Co Ltd | Bepaling van met tumor gepaard gaande glycobrug, kankerdiagnose en pakketten voor kankerdiagnose. |
| US4569909A (en) * | 1982-06-03 | 1986-02-11 | Seitetsu Kagaku Co., Ltd. | Process for preparing uridine diphosphate-N-acetylgalactosamine |
| US4797356A (en) * | 1985-12-02 | 1989-01-10 | Konishiroku Photo Industries, Co., Ltd | Monoclonal antibodies specific to glactosyltransferase isoenzyme II and their use in cancer immunoassays |
-
1986
- 1986-12-16 JP JP29933386A patent/JPS63152998A/ja active Granted
-
1987
- 1987-12-16 EP EP87118665A patent/EP0272603A3/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315761A (ja) * | 1989-03-15 | 1991-01-24 | Sumitomo Seika Chem Co Ltd | 糖転移酵素の活性測定方法およびその用途 |
| JPH03140865A (ja) * | 1989-10-27 | 1991-06-14 | Kosumitsuku:Kk | 悪性腫瘍の判別方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0272603A2 (en) | 1988-06-29 |
| JPH0548120B2 (https=) | 1993-07-20 |
| EP0272603A3 (en) | 1989-04-26 |
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