JPS63141909A - Emulsion-type cosmetic - Google Patents

Abstract

PURPOSE:To provide an O/W-type cosmetic produced from laminin, a water-soluble salt of a carboxyvinyl polymer, a surfactant, an oily substance and water and having excellent storage stability, skin safety and practical properties, etc. CONSTITUTION:The objective O/W-type emulsion cosmetic is composed of 0.2-5.0wt.% laminin, 0.05-2.0wt.% water-soluble salt of carboxyvinyl polymer, 2.0-7.0wt.% surfactant, 10-70wt.% oily substance (e.g. oil and fat, wax, hydro carbon, etc.) and 20-85wt.% water. The cosmetic has high storage stability, excellent safety to skin and practical characteristics such as spreadability, viscosity, moisture-retaining property, water-resistance, etc. Laminin is a non- collagen protein existing in the basement membrane of epidermis, etc., of mammals and can be produced from the epidermis by conventional protein- extraction process.

Description

Detailed Description of the Invention (Technical Field) The present invention provides an oil phase/aqueous phase type (hereinafter referred to as O/ The present invention relates to an emulsified cosmetic having a dispersion state (abbreviated as W-type).

More specifically, storage stability, skin safety and practical properties (
The present invention relates to emulsified cosmetics with excellent spreadability, viscosity, moisturizing properties, water resistance, etc.

(Prior Art) Conventionally, various thickeners (gelling agents) have been used to improve the storage stability of O/W type emulsion compositions.

For example, water-soluble thickeners include vegum, xanthan gum, pectin, carrageenan, carboxyvinyl polymer salts, etc., and oil-soluble thickeners include organic bentonite, sig sugar fatty acid ester, and dextrin fatty acid ester. etc. are known.

However, emulsified compositions containing these thickeners alone do not have sufficient storage stability;
It was essential to use a high concentration of nonionic and cationic surfactants.

In addition, in the case of emulsified cosmetics in which an emulsified composition containing a high concentration of this surfactant is applied as a cosmetic base, (a) "elongation" (spreadability) is important in terms of practical properties. ) is bad, (b) has a "sticky feeling" and "sliminess" (poor viscosity), (c) has poor water resistance, and makeup tends to come off due to sweating, etc. had various problems such as poor skin safety, and were not necessarily satisfactory.

(Disclosure of the Invention) Therefore, as a result of intensive research into emulsified cosmetics that incorporate various thickeners, the present inventors discovered that laminin, a water-soluble salt of carboxyvinyl polymer, an oily substance, and a surfactant. It was discovered that a ◯/W type emulsified cosmetic prepared by blending .

(Objective of the Invention) That is, the object of the present invention is to provide O2 with excellent storage stability, skin safety, and practical properties (spreadability, viscosity, moisture retention, and water resistance).
/W type emulsion type cosmetics.

(Structure of the Invention) The present invention is an oil phase/water phase type emulsified cosmetic comprising laminin, a water-soluble salt of a carboxyvinyl polymer, a surfactant, an oily substance, and water.

(Specific Description of Structure) Laminin according to the present invention is a non-collagen protein present in basement membranes such as the epidermis of mammals, and can be extracted from biological samples containing basement membranes. It is composed of protein molecules with molecular weights of 220,000, 440,000, and 850,000, and is expected to be found in the basement membrane, and to play a role, along with collagen, in adhesion between epidermal cells and the basement membrane.

[European Journal of Biochemistry, No. 102, No. 5, pp. 195-201.

1979 CEur, J. Biochem, 1
02, 195-201.1979), Front Matrix Biology Vol. 7 No. 130-14101
979 (Front, Matrix, Biol,
, 7, 130-141.1979), Journal of Biological Chemistry, Vol. 254, No. 9
pp. 933-9937, 1979 (J. Biol.
Chem, +258.9933-9937. 1979
), etc.) Laminin is extracted from the basement membrane by a normal protein extraction method. For example, laminin can be obtained from the epidermis of a minipig as follows.

(Experiment example)...See Table 1■ After washing the dorsal skin of 100 mini pigs (formerly Nature Pigs) and cutting their hair,
The upper layer of the skin, including the basement membrane, is peeled off using a medical dermatome to obtain 2.5 kg of a sample for laminin extraction.

■ 7.5 kg of 3.4 mol sodium chloride (NaCj aqueous solution (0.05 mol Tris-HCl buffer - adjusted to PH = 7.4) was added to this sample, and the mixture was stirred at a temperature of 4°C for 30 minutes. , liberate blood-derived proteins,
; Exclude this by door to door.

■ 0.5 mol NaC, which is 10 times the amount of the obtained residue.
1 aqueous solution (PH = 7.4 UR finished) and heated to 4°C.
The mixture is stirred once and filtered to obtain an extract containing laminin.

Repeat this extraction operation once more and combine the extracts.

■ Add NaC1 to this extract to reduce the NaC1 concentration to 1
．． Adjust to 7 mol, and remove the generated collagen precipitate by centrifugation.

(2) Add ammonium sulfate to the obtained extract to make a saturated solution, and obtain a precipitate containing laminin by centrifugation.

■ Dissolve this precipitate in 31 0.05 molar Tris-HCl buffer (PH = 8.6), put it into a dialysis tube, and then dissolve it in 0.05 molar Tris-HCl buffer containing 2 molar concentration of phosphorus (PH = 8.6). Dialyze against PH-8,6) for 2 days.

■ This I precipitate was equilibrated with 2 molar urea-0.05 molar Tris-HCl buffer (PH = 8.6).
AF, pass through a cellulose column (10 cm x 60 cm) and aspirate proteins other than laminin.

(2) This permeate was dialyzed against distilled water, condensed (4), and lyophilized to obtain 7.5 g of mini-butalaminin.

Next, sheep laminin 8.7 was obtained from the back skin of grasses in the same manner as the extraction method described above.

Next, to investigate the properties of laminin as a protein, we analyzed its constituent amino acids.

As a result, as shown in Table 2, minipig laminin and sheep laminin were found to be laminin-specific proteins consisting of similar amino acid #1lJl compositions. , 2 was done.

Table 1 (Laminin extraction method) Residue 113 Fluid (blood protein) extract Residue Precipitate Dialysis fluid Table 2 (Amino acid composition of laminin) The amount of laminin blended is 0 based on the total amount of the cosmetic.
．． 2 to 5.0% by weight (hereinafter abbreviated as wt%) is preferred; less than 0.2WL% results in poor storage stability;
5. If it exceeds Q w t%, the appearance characteristics and practical properties such as viscosity of the dosage form will deteriorate.

The water-soluble salt of carboxyvinyl polymer used in the present invention is a well-known compound. It is a salt consisting of an acid-type carboxyvinyl polymer (for example, Carpopol 940 manufactured by Gutdrich, Inc.) and a basic substance such as caustic soda, caustic potash, or alkanolamine, and the pH value of its aqueous solution is 5.0.
~8.0.

The blending amount of the water-soluble salt of carboxyvinyl polymer is 0.05~'l, Q w based on the total amount of the cosmetic.
If the aO value is less than 05 wt%, the storage stability will not be sufficient, and if it exceeds 2.0% by weight, the appearance characteristics and practical properties of the dosage form will deteriorate.

The surfactant is an emulsifier commonly used in emulsified cosmetics, and one or more of anionic, cationic, and nonionic surfactants may be used.

The blending amount is 2.0 to 7.0% based on the total amount. Q
wt% is preferred.

The oily substance used in the present invention is a known oily raw material for cosmetics, and includes, for example, oils and fats, waxes, hydrocarbons, ester oils, higher alcohols, and grade fatty acids.

One or more of these oily substances are selected and applied depending on the type, dosage form, purpose, etc. of the cosmetic. The amount of blending is usually 1% based on the total amount of the cosmetic.
0 to 70W (.

In addition, the water is purified water that is usually used, such as deionized purified water treated with an ion exchange resin or distilled water, and the blending amount is 20 to 20 g based on the total amount.
The content is preferably 5 wt%.

In addition to the above, the emulsified cosmetic of the present invention may appropriately contain ordinary cosmetic raw materials such as fragrances, preservatives, humectants, pigments, pigments, etc. within the range that achieves the purpose of the present invention. can do.

In addition, the emulsified cosmetics of the present invention include skin creams, skin emulsions, Masosano creams, cleansing creams,
It is applied to hair creams, makeup bases, hand creams, and many other emulsion-like or cream-like cosmetics.

(Example) The present invention will be explained below with reference to Examples.

The test methods for storage stability, skin safety, and practical properties described in the Examples are as follows.

(1) Storage stability test After storing the sample in a constant temperature room at 45°C for 3 months, observe the appearance and emulsion width of the sample, and if no abnormality is observed (emulsification state and particles are uniform) If any abnormality was observed (oil separated, particles became coarse, etc.), it was evaluated as poor.

(2) Skin safety test Samples o and o s were applied to the skin of the pressure side of the forearm of 25 subjects.
g was applied for 24 hours for occlusion using a batch test adhesive with a circular lint cloth of diameter 1.0 cm.
The skin condition of 25 subjects was evaluated for each sample according to the following criteria.

The results of the evaluation were determined based on the stronger reaction between 1 hour and 24 hours after the removal of the bandage, and were expressed as the number of people with an evaluation of (±) or below.

(3) Practical property test (spreadability, viscosity, moisture retention, water resistance) After 20 test subjects used the sample for 10 days, the properties of the sample were evaluated.

The test results for test items such as spreadability, viscosity, moisture retention, and water resistance were evaluated as follows: ``Good spread when applied,'' ``No sticky or slimy feeling,'' and ``Less moisturized skin.'''', and the number of people who answered ``My makeup doesn't come off easily or my hair gets messy due to sweat.''

Examples 1 to 6, Comparative Examples 1 to 4 (0/W type skin cream) Each O/W type skin cream was prepared by blending various thickeners, etc. as shown in Table 3 as shown below. Sukiskin Cream was manufactured and the various tests described above were conducted.

In addition, in Comparative Example 1, the amount of POE sorbitan monooleate (20EO), which is the β) component, was increased to 3.5 wt.
In addition to the above table, cetyl alcohol sulfate ester Na salt was added to the Ω component in an amount of Q, 5 wt%.

(2) Preparation method Mix the four ingredients into the 0th order part, (c), 0th order part each 8
Each skin cream was prepared by mixing the first, (5) and zeroth components heated and dissolved at 0° C. and cooling to 30° C. with stirring.

(3) Properties As shown in Table 3, Comparative Example 1, in which a high concentration of surfactant was blended, had good storage stability, but did not yield favorable results in terms of skin safety and practical properties. In addition, Comparative Examples 2 to 4, in which laminin or carboxyvinyl polymer salts were individually blended, were clearly inferior in storage stability.

On the other hand, the emulsified cosmetic composition of the present invention showed excellent properties in various tests.

Examples 7 to 8, Comparative Example 5 [0/W type makeup base] In the same manner as in Example 1, each 0/W type makeup base was prepared with the following composition, and various tests were carried out (2 ) Properties As shown in Table 4, Examples 7 and 8, which are emulsified cosmetics of the present invention, exhibited excellent properties in various tests.

(Effects of the Invention) As described above, the present invention provides a useful emulsified cosmetic with excellent practical properties such as storage stability, skin safety, spreadability, viscosity, moisturizing property, and water resistance. That is clear.

Claims (1)

[Claims] laminin and a water-soluble salt of carboxyvinyl polymer;
An oil phase/water phase type emulsified cosmetic made by blending a surfactant, an oily substance, and water.