JPS6244173A - 培養方法および装置 - Google Patents
培養方法および装置Info
- Publication number
- JPS6244173A JPS6244173A JP60180983A JP18098385A JPS6244173A JP S6244173 A JPS6244173 A JP S6244173A JP 60180983 A JP60180983 A JP 60180983A JP 18098385 A JP18098385 A JP 18098385A JP S6244173 A JPS6244173 A JP S6244173A
- Authority
- JP
- Japan
- Prior art keywords
- liquid medium
- cells
- culture
- container
- culture container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title description 19
- 239000007788 liquid Substances 0.000 claims abstract description 143
- 239000002245 particle Substances 0.000 claims description 41
- 230000001419 dependent effect Effects 0.000 claims description 19
- 230000002062 proliferating effect Effects 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 238000012136 culture method Methods 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 abstract description 25
- 230000005484 gravity Effects 0.000 abstract description 20
- 239000002609 medium Substances 0.000 description 144
- 210000004027 cell Anatomy 0.000 description 117
- 241000196324 Embryophyta Species 0.000 description 69
- 238000012258 culturing Methods 0.000 description 15
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 210000004748 cultured cell Anatomy 0.000 description 7
- 206010020649 Hyperkeratosis Diseases 0.000 description 5
- 238000007599 discharging Methods 0.000 description 5
- 239000004744 fabric Substances 0.000 description 5
- 238000010008 shearing Methods 0.000 description 5
- 239000013543 active substance Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 210000002950 fibroblast Anatomy 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 210000001938 protoplast Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000699802 Cricetulus griseus Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000003292 kidney cell Anatomy 0.000 description 2
- 210000005265 lung cell Anatomy 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 description 1
- 208000014697 Acute lymphocytic leukaemia Diseases 0.000 description 1
- 244000307697 Agrimonia eupatoria Species 0.000 description 1
- 235000016626 Agrimonia eupatoria Nutrition 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 241000282552 Chlorocebus aethiops Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 240000006497 Dianthus caryophyllus Species 0.000 description 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 229910000976 Electrical steel Inorganic materials 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001071917 Lithospermum Species 0.000 description 1
- 229910001209 Low-carbon steel Inorganic materials 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000282577 Pan troglodytes Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 208000002495 Uterine Neoplasms Diseases 0.000 description 1
- 244000172533 Viola sororia Species 0.000 description 1
- 208000024447 adrenal gland neoplasm Diseases 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 210000003953 foreskin Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000006247 magnetic powder Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 208000010916 pituitary tumor Diseases 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 206010046766 uterine cancer Diseases 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
- 229910000859 α-Fe Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60180983A JPS6244173A (ja) | 1985-08-20 | 1985-08-20 | 培養方法および装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60180983A JPS6244173A (ja) | 1985-08-20 | 1985-08-20 | 培養方法および装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6244173A true JPS6244173A (ja) | 1987-02-26 |
| JPH0534944B2 JPH0534944B2 (enrdf_load_stackoverflow) | 1993-05-25 |
Family
ID=16092691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60180983A Granted JPS6244173A (ja) | 1985-08-20 | 1985-08-20 | 培養方法および装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6244173A (enrdf_load_stackoverflow) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443985A (en) * | 1993-07-22 | 1995-08-22 | Alberta Research Council | Cell culture bioreactor |
| KR100848938B1 (ko) | 2007-04-20 | 2008-07-29 | 코아스템(주) | 세포배양관 및 이를 포함하는 대량 세포배양장치 |
| JP2010119323A (ja) * | 2008-11-18 | 2010-06-03 | Ihi Corp | 微生物濃縮装置 |
| JP2011200128A (ja) * | 2010-03-24 | 2011-10-13 | Ihi Corp | 微生物濃縮装置 |
| US11981884B2 (en) | 2022-10-17 | 2024-05-14 | Upside Foods, Inc. | Pipe-based bioreactors for producing comestible meat products and methods of using the same |
| US12256761B2 (en) | 2019-11-20 | 2025-03-25 | Upside Foods, Inc. | Apparatuses and systems for preparing a meat product |
-
1985
- 1985-08-20 JP JP60180983A patent/JPS6244173A/ja active Granted
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443985A (en) * | 1993-07-22 | 1995-08-22 | Alberta Research Council | Cell culture bioreactor |
| KR100848938B1 (ko) | 2007-04-20 | 2008-07-29 | 코아스템(주) | 세포배양관 및 이를 포함하는 대량 세포배양장치 |
| WO2008130119A1 (en) * | 2007-04-20 | 2008-10-30 | Corestem Co., Ltd. | Cell culture tube and multiple roller tube cell culture apparatus including the same |
| US8815578B2 (en) | 2007-04-20 | 2014-08-26 | Corestem Co., Ltd. | Cell culture tube and multiple roller tube cell culture apparatus including the same |
| US9493734B2 (en) | 2007-04-20 | 2016-11-15 | Corestem Co., Ltd. | Cell culture tube and multiple roller tube cell culture apparatus including the same |
| JP2010119323A (ja) * | 2008-11-18 | 2010-06-03 | Ihi Corp | 微生物濃縮装置 |
| JP2011200128A (ja) * | 2010-03-24 | 2011-10-13 | Ihi Corp | 微生物濃縮装置 |
| US12256761B2 (en) | 2019-11-20 | 2025-03-25 | Upside Foods, Inc. | Apparatuses and systems for preparing a meat product |
| US11981884B2 (en) | 2022-10-17 | 2024-05-14 | Upside Foods, Inc. | Pipe-based bioreactors for producing comestible meat products and methods of using the same |
| US11987778B2 (en) | 2022-10-17 | 2024-05-21 | Upside Foods, Inc. | Methods of using pipe-based bioreactors for producing comestible meat products |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0534944B2 (enrdf_load_stackoverflow) | 1993-05-25 |
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