JPS6240287A - Preservation of mushroom hyphae - Google Patents

Abstract

PURPOSE:To stop the growth of mushroom hyphae in the state of secondary hyphae and artificially control the period from inoculation of seed fungi to the harvesting, by keeping a culture medium spread with the mushroom hyphae in an atmosphere under a low partial pressure of oxygen. CONSTITUTION:Mushroom species related to wood destroying fungi, e.g. Panus ostreatus, Pholiota nameko, SHIITAKE mushroom etc., are subjected to culture medium adjusting and sterlization in a pressurized vessel according to the respective fungal species by a well-known method, and seed fungi as a secondary hyphae are inoculated thereinto the carry out cultivation until the culture medium is spread with the hyphae. Operation to reduce partial pressure of oxygen is performed in the state of spread secondary hyphae to keep the atmosphere of the low partial pressure of oxygen under <=100mmHg reduced pressure or <=38mmHg partial pressure of oxygen under atmospheric pressure. Thereby, the growth of the mushroom hyphae is stopped in the state of secondary hyphae to control the period to harvesting of fruit bodies.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a method for terminating the growth of mushrooms in the form of secondary hyphae and preserving them.

Conventional technology and its problems Mushroom ode, which is made of wood, matures into a mycospore in the kuminospore of the fruiting body, and then undergoes repeated cell division under proper conditions of watering and humidity to become primary mycospores. Flt length.

Furthermore, this secondary mycelium conjugates with a primary mycelium of the opposite sex to become a secondary mycolia, which elongates into a tree or culture medium to form a primordium, which enlarges and becomes a fruiting body.

In such a living environment of mushrooms, it is relatively easy to stop their growth and preserve them with Kumiko Hayashi's +lJs, but conventionally, mushroom cultivation is done by using seed fungi that are pure cultured secondary cells. In order to grow Sakae II in a medium, once the inoculum is inoculated, it takes several days from inoculation to harvest (it is difficult to operate) Ta.

The present invention focuses on the fact that the metabolism of secondary mycelium is significantly reduced under low oxygen partial pressure, and there is relatively little contamination by bacteria.The present invention controls the harvesting period of fruiting bodies and utilizes the storage period to ship them to distant locations. This was done with the purpose of making it possible to display it as a product at stores.

Solution to the tJ112N point: A medium infested with mushroom mycelia is placed under a low oxygen partial pressure atmosphere.
cii preferably under pressure in the range below /θOm Hg,! By setting the elementary partial pressure to J, l # Hg or less, the growth of the mushroom mycelium is stopped in the form of secondary mycelium, and the period from inoculation of the first species M to harvesting of the fruiting body is artificially controlled. .

Example The method for preserving the mushroom bellows will be explained in detail below.

The mushroom to be tested in the present invention may be any of oyster mushrooms, nameko mushrooms, shiitake mushrooms, etc., and the mushroom species are not limited.

After adjusting the culture medium and sterilizing it in an autoclave according to a well-known method according to each mushroom species, 9 seedlings are harvested as a secondary yarn and cultured until it spreads in the culture medium.

Next, the oxygen partial pressure is lowered in the bark state where the secondary direct lineage is growing and passing through to stop the growth and preserve it.There are vacuum methods and gas replacement methods for lowering the oxygen partial pressure.

When cultivating oyster mushrooms using the vacuum method, the vacuum chamber in the cultivation bottle is kept at a storage temperature within the range of total gas pressure J~/σQ m Hg~J
j If you use alJI that does not freeze at ℃, the secondary hyphae will grow.
Growth can be stopped for at least 74 days.

However, when storing for a long time in a sealed container,
Carbon gas in the cultivation bottle gradually increases, and it is known that under carbon gas lit of o, i < or more, aerial mycelium grows and becomes an obstacle to fruiting body ablation. It is preferable not to include a carbon dioxide absorbent.

On the other hand, in the case of the gas replacement method, the gas used may be an inert gas such as nitrogen or argon.

In cultivation of oyster mushrooms using the substitution method, if the oxygen temperature change in the culture bottle is kept in the range of σ to j and the darkness in the storage bottle is kept in the range of Growth can be stopped for at least 74 days. In the case of the gas replacement method as well, it is preferable to include a carbon dioxide absorbent in the same manner as in the vacuum method.

Below are the actual results obtained using the vacuum method and gas replacement method for oyster mushroom cultivation.

1 month of marriage 11) Cultivation bottle tσ0CCp, p bottle 12) Medium Mixed proboscis of sawdust, rice bran, and yield enhancer.

The dry weight ratio of scum and rice bran is dσ:qθ
A yield enhancer of ggDs was added to each bottle.

(3) Warm culture @Iff: J g'c Growth Warm temperature: /2°C There are three possible times to perform the growth stop operation as shown in Figure @7, but at which time should you perform the growth stop operation? However, it is possible for growth to stop. The stopping time is immediately after the completion of culture.
Stop time 0 indicates immediately before primordium formation, and stop time O indicates immediately after primordium formation.

Within the range of storage temperature, growth can be stopped in the state of secondary hyphae for at least 81 days.

(2) In the vacuum method with a storage temperature of 2°C, it is possible to stop growth for up to Jt days in the state of J secondary hyphae. As a result, the number of days from the end of culture to harvest was 37 days, and no significant difference was observed among the daily cultivation method, vacuum method, and NJill conversion method, which all used growth suspension operations. will not decrease.

(4) The incidence of bacterial contamination is extremely low regardless of whether the Oku method or the Nko@Ko method is added to the conventional cultivation method.

[Brief explanation of drawings]

Figure @/ is a block explanatory diagram showing the method for preserving mushroom mycelia of the present invention, and Figure @2 is a publicly known block explanatory diagram. Figure 1 □ [ ; Procedural amendment (voluntary) 1. Description of the case Japanese Patent Application No. 179079 No. 2, title of the invention: Mushroom mycelium preservation method 3, person making the amendment Relationship with the case Applicant: Hitachi Kiden Hitachi Kiden Kogyo Co., Ltd. 4, Agent: 1 Nishihonmachi, Nishi-ku, Osaka City No. 5 Fuji Building New Building, 2-8 Chome (
6383) Patent attorney Ike 1) Yoshio Yomo (foreign name)
1. ) 5. Date of directive or notification: 1939, month, day 6. Full text of the specification to be amended 7. Contents of the amendment as attached. 1. Title of the invention: Method for preserving mushroom mycelium 2. Scope of claims (1) A method for preserving mushroom hyphae, which is characterized in that the growth of mushroom hyphae is stopped and preserved with the good news of secondary stuttering by creating a low oxygen partial pressure atmosphere in a medium in which mushroom hyphae are prevalent. +21 A method for preserving mushroom mycelia according to claim 1, in which a low-oxygen molecular atmosphere is maintained under a reduced pressure of /θσ-Hg or less; +3) A method for preserving mushroom mycelia by sealing in Nu gas; Partial pressure J I
``Method for preserving mushroom hyphae as described in the claims @/ in order to reduce the hyphae to Hg or less. 3. Detailed description of the invention Industrial field of application The present invention is a method for preserving mushroom hyphae by reducing the growth of mushroom hyphae to secondary hyphae. ,
It concerns how to preserve it. Conventional technology and its problems Mushroom shoulder, which is a wood-perishing fungus, is a wood-perishing fungus that matures the muntio spores in the fruiting body and repeats saccular division under appropriate warm and humid conditions. Grows into primary hyphae. Furthermore, this secondary hyphae merges with the primary hyphae of the opposite sex to form a secondary hyphae, which elongates in the tree or culture medium to form a stock base, which enlarges and becomes a fruiting body. In the life cycle of mushrooms, it is relatively easy to stop growth and preserve them in the state of basidioelectrons, but conventionally, mushroom cultivation involves cultivating seedlings with pure culture of secondary mycelia. In order to inoculate the culture medium and grow vegetatively, once the inoculum is inoculated, the number of days required from 1' seed to harvest depends on the mushroom, and it is difficult to artificially manipulate the growth rate t. The present invention focuses on the fact that under low oxygen partial pressure, the metabolism of secondary hyphae is significantly reduced, and there is relatively little contamination by bacteria.The present invention controls the fruiting body harvest period and utilizes the storage period to facilitate shipment to distant locations. This was done for the purpose of making it possible to display it as a product in a store. The solution to the problem is to place the culture medium infested with mushroom mycelia under a low oxygen partial pressure atmosphere,
Desirably, the growth of the mushroom hyphae is stopped in the state of secondary hyphae by reducing the oxygen partial pressure to Jt-Hg or less under a reduced pressure of /σσ-sHg or less or atmospheric pressure, and fruiting bodies are harvested from inoculation with one species of fungus. Period 1 ■ is artificially controlled. In the following example, a method for preserving one string of end-grown mushrooms will be described. Mushrooms to be tested include oyster mushrooms, nameko mushrooms, and shiitake mushrooms, which are related to the extent of wood decay. According to well-known methods according to each mushroom species, culture medium 1
After conditioning and sterilizing in a pressurized can, seed fungi are layered as secondary mycelia.
Cultivate until the cells are spread in the medium. Next, in a state where secondary hyphae are widespread, an operation to lower the oxygen partial pressure is performed to stop growth and preserve the hyphae. There are two methods for lowering the oxygen partial pressure: the vacuum method and the gas @ night method. When cultivating oyster mushrooms using the reduced pressure method, the vacuum inside the cultivation bottle is reduced to full gas pressure! If the storage temperature is maintained in the range of ~/00 #Hg in the range of 12 to 10C without freezing, growth can be stopped in the state of secondary hyphae for at least 74 days. However, when storing for a long time in a sealed cultivation bottle,
It is known that carbon gas art- in the cultivation bottle gradually increases, and under carbon gas conditions of 0.2 or more, aerial mycelium grows and becomes an obstacle to fruiting body yield of 1 fI. It is preferable to enclose a carbon dioxide gas absorbent therein. On the other hand, Gas W! In the case of kanpo, the gas used may be an inert gas such as nitrogen or argon. In oyster mushroom cultivation using the M method, oxygen 7a in the cultivation bottle
Growth can be stopped for at least 74 days in the condition of the secondary layer yarn by keeping the fibers in the range of 0 to 0 and the storage temperature in the range of -2-C. It is preferable to enclose a carbon dioxide gas absorbent in each container.The following are the experimental results using the reduced pressure method and the gas replacement method in cultivating oyster mushrooms.1 Cultivation 1 (1) Cultivation bottle toac Polypropylene Bottle (2) A mixture of medium waste, rice bran, and a yield increaser. The dry weight ratio of waste and rice bran was set to 1゛σ: 40, and the yield increaser was added at a rate of #gDS per chin. (3) Temperature culture section*: J 4/'C growth temperature 111': /J℃
As shown in Figure 1, there are three possible timings for performing the growth stop operation. Growth can be stopped by performing the growth stop operation at any time. The stop time ■ indicates immediately after the completion of cultivation, the stop time O indicates immediately before the formation of the primordium, and the stop time O indicates immediately after the formation of the primordium. Within the temperature range, growth can be stopped for at least 74 days in the condition of secondary hyphae. (2) In the reduced pressure method with J'C as the storage voltage, it is possible to stop growth for up to Eat days in the condition of one secondary thread. As a result, the number of days from the end of culture to harvest is 37 days, which can be extended by the number of days for which the growth stop operation was performed. (3) There was no noticeable difference in yield between the conventional cultivation method without growth-stopping operation, the depressurization method of the present invention, and the N-coil cooling method. (4) The incidence of bacterial contamination is extremely low even if either the depressurization method or the nitrogen replacement method is added to the conventional cultivation method. 4. Brief explanation of the drawing The block explanatory diagram showing the method for preserving mushroom hyphae of the present invention, Figure @2, is a known block jQ I'JJ diagram.

Claims (3)

[Claims] (1) A method for preserving mushroom mycelium, which comprises suspending the growth of mushroom mycelia in the state of secondary hyphae and preserving the medium in a medium infested with mushroom mycelia under a low oxygen partial pressure atmosphere. (2) The method for preserving mushroom mycelium according to claim 1, wherein the low oxygen partial pressure atmosphere is kept under reduced pressure of 100 mmHg or less. (3) By filling in N_2 gas, the oxygen partial pressure can be reduced to 38
A method for preserving mushroom mycelium according to claim 1, wherein the temperature is below mmHg.