JPS62275688A - Production of pyruvic acid by fermentation - Google Patents
Production of pyruvic acid by fermentationInfo
- Publication number
- JPS62275688A JPS62275688A JP27531086A JP27531086A JPS62275688A JP S62275688 A JPS62275688 A JP S62275688A JP 27531086 A JP27531086 A JP 27531086A JP 27531086 A JP27531086 A JP 27531086A JP S62275688 A JPS62275688 A JP S62275688A
- Authority
- JP
- Japan
- Prior art keywords
- pyruvic acid
- torulopsis
- producing
- pyruvate
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 229940107700 pyruvic acid Drugs 0.000 title claims abstract description 15
- 238000000855 fermentation Methods 0.000 title claims description 10
- 230000004151 fermentation Effects 0.000 title claims description 10
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 18
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims abstract description 13
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229960002685 biotin Drugs 0.000 claims abstract description 9
- 235000020958 biotin Nutrition 0.000 claims abstract description 9
- 239000011616 biotin Substances 0.000 claims abstract description 9
- 239000011721 thiamine Substances 0.000 claims abstract description 7
- 235000019157 thiamine Nutrition 0.000 claims abstract description 7
- 241000222126 [Candida] glabrata Species 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 229960003495 thiamine Drugs 0.000 claims abstract description 4
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims abstract description 4
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 15
- 229940076788 pyruvate Drugs 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 abstract description 5
- 229930003231 vitamin Natural products 0.000 abstract description 5
- 235000013343 vitamin Nutrition 0.000 abstract description 5
- 239000011782 vitamin Substances 0.000 abstract description 5
- 239000006227 byproduct Substances 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- -1 biotin for growth Natural products 0.000 abstract description 2
- 239000000047 product Substances 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229960003512 nicotinic acid Drugs 0.000 description 4
- 235000001968 nicotinic acid Nutrition 0.000 description 4
- 239000011664 nicotinic acid Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 235000008160 pyridoxine Nutrition 0.000 description 3
- 239000011677 pyridoxine Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 229940011671 vitamin b6 Drugs 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 2
- 229960004172 pyridoxine hydrochloride Drugs 0.000 description 2
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 2
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000002897 organic nitrogen compounds Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000011118 potassium hydroxide Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Abstract
Description
【発明の詳細な説明】
３、発明の詳細な説明
〈産業上の利用分野〉
本発明は発酵法によるピルビン酸の製造法に関するんも
のである。[Detailed Description of the Invention] 3. Detailed Description of the Invention (Field of Industrial Application) The present invention relates to a method for producing pyruvic acid by a fermentation method.
ピルビン酸は生体代謝の重要な中間体であり、各硬固・
、＠薬などの有効な合成原料で必るのみならず酸素法に
よるＬ−トップ１〜フアン、Ｌ−システィン、し−チロ
シンなどのアミノ酸合成の主要原料で必る。よって安価
に製造しくｑれば、種々の合成原料として有用で必る。Pyruvate is an important intermediate in biological metabolism, and is
It is essential not only as an effective raw material for the synthesis of drugs, but also as a main raw material for the synthesis of amino acids such as L-top 1-fan, L-cysteine, and tyrosine by the oxygen method. Therefore, if it can be produced at low cost, it will be useful as a raw material for various synthetics.
〈従来の技術〉
従来、発酵法によるピルビン酸の製造法としては、サツ
カロミセス属およびキャンディダ屈などの酵母菌や担子
菌類または特殊なバクテリアによる方法が知られている
（特公昭５７−７９６号公報、特開昭５７−’１５９４
９２号公報等）。<Prior art> Conventionally, as a method for producing pyruvic acid by fermentation, a method using yeasts such as Satucharomyces and Candida spp., basidiomycetes, or special bacteria is known (Japanese Patent Publication No. 57-796). , Japanese Patent Publication No. 57-'1594
Publication No. 92, etc.).
く本発明が解決しようとする問題点〉
しかしながら、かかる従来方法は副生物が多かったり、
または、収率・収量が十分でなかったりしたため工業的
に有利な方法とは言えなかった。Problems to be Solved by the Present Invention> However, such conventional methods produce many by-products,
Alternatively, the yield and yield were insufficient, so it could not be said to be an industrially advantageous method.
く問題点を解決するための手段および作用〉したがって
本発明者らは上記問題点を解決することのできる、新規
な、発酵法によるピルビン酸生産菌を見出すことを目的
として鋭意研究したところ、トルロプシス居に属する酵
母菌が優れた効果を奏することを見出し本発明に到達し
た。Means and Action for Solving the Problems> Therefore, the present inventors conducted intensive research with the aim of finding a new pyruvate-producing bacterium by fermentation that could solve the above problems, and found that Torulopsis The present invention was achieved by discovering that yeast bacteria belonging to the genus genus spp. have excellent effects.
すなわち、本発明の上記目的は、トルロプシス属に属す
るピルビン酸生産菌を培養することにより培地中にピル
ビン酸を生成蓄積させ、これを採取することにより、達
成されるのである。That is, the above-mentioned object of the present invention is achieved by culturing pyruvate-producing bacteria belonging to the genus Torulopsis to produce and accumulate pyruvate in a medium, and then collecting the pyruvate.
トルロプシス属の酵母を用いた発酵法によるピルビン酸
の著量の蓄積はこれまで知られていなかった。The accumulation of significant amounts of pyruvate by fermentation using yeast of the genus Torulopsis has not been known so far.
以下、本発明の構成を詳細に説明する。Hereinafter, the configuration of the present invention will be explained in detail.
本発明に厄いられるピルビン酸生産菌はピルビン酸生成
能を荷するトルロプシス属に属する酵母菌であればいか
なるものでおってもよいが、望ましくは各種のビタミン
を要求するもの、特にチアミン、ナイアシン、ピリドキ
シン、ビオチンなどを単独もしくは併せて要求するもの
が好適である。The pyruvate-producing bacteria troubled by the present invention may be any yeast belonging to the genus Torulopsis that has the ability to produce pyruvate, but preferably those that require various vitamins, especially thiamin and niacin. , pyridoxine, biotin, etc. alone or in combination are suitable.
本発明で用いられるピルビン酸生産菌としては、例えば
、トルロプシス・グラブラータ（Ｔｏｒｕｌｏｐｓｉｓ
ｇｌａｂｒａｔａ）（）ＦＯ０００５）（チアミン
、ビオチン、ピリドキシンおよびナイアシン要求）、ト
ルロプシス・メタノロベラセンス（Ｔｏｒｕ Ｉ ｏｐ
ｓ ｉＳ ｍｅｔｈａｎｏｌｏＶｅｓｃｅｎｓ）（△
ＴＣＣ２６１７６＞（チアミンおよびビオチン要求）な
どが挙げられる
本発明で用いられる培地は発酵に通常使用される炭素源
、窒素源、無機塩類、ビタミン類などを程よく含有する
ものであればよいが、炭素源としては、グルコースなど
の糖質、有機酸、エタノール、メタノールなどの使用酵
母菌が利用し得るものが使用される。窒素源として硫安
、硝安、塩安、尿素、ペプトン、肉エキス、味液、その
他の有機および無機窒素化合物が使用されるが、望まし
くはアミノ酸をバランスよく含む有機窒素化合物がよい
。無機塩類としてはリン酸カリウム、ＦｉＡ酸マグネシ
ウム、鉄、マンガン、その他の無機塩類が用いられ、ざ
らに必要に応じてチアミン、ナイアシン、ピリドキシン
、ビオチンなどの要求ビタミン、またはこれらを含有す
る酵母エキス、コーン・スチープ・リカー、その他の天
然物を添加した培地を使用すればよい。Examples of the pyruvate-producing bacteria used in the present invention include Torulopsis glabrata.
glabrata) ()FO0005) (requires thiamine, biotin, pyridoxine and niacin), Torulopsis methanoloverascens (Toru I op
s iS methanoloVescens) (△
TCC26176> (thiamin and biotin requirement), etc. The culture medium used in the present invention may contain moderate amounts of carbon sources, nitrogen sources, inorganic salts, vitamins, etc. normally used in fermentation. For example, carbohydrates such as glucose, organic acids, ethanol, methanol, etc., which can be used by the yeast used, are used. As a nitrogen source, ammonium sulfate, ammonium nitrate, ammonium chloride, urea, peptone, meat extract, flavor liquid, and other organic and inorganic nitrogen compounds are used, and organic nitrogen compounds containing amino acids in a well-balanced manner are preferable. Potassium phosphate, magnesium FiA, iron, manganese, and other inorganic salts are used as inorganic salts, and if necessary, required vitamins such as thiamin, niacin, pyridoxine, and biotin, or yeast extract containing these, A medium supplemented with corn steep liquor or other natural products may be used.
培養中はピルビン酸の生成蓄積にともない、ρ１」の低
下が起こるので炭酸カルシウム、苛性ソーダ、苛性カリ
などのアＪレカリでｐｌ」３〜７に調節することがピル
ビン酸生産には有効である。培養中の温度は２２℃〜３
２°Ｃが適当でおる。培養終了後、系内に蓄積したピル
ビン酸は常法により、単離採取することができる。例え
ば、酸性エーテル抽出、フェニルヒドラゾン化して沈澱
単離する方法なども採用することができる。During cultivation, as pyruvate is produced and accumulated, ρ1' decreases, so it is effective for pyruvic acid production to adjust pl' to 3 to 7 with an alimentary agent such as calcium carbonate, caustic soda, or caustic potash. The temperature during culturing is 22℃~3
2°C is suitable. After completion of the culture, pyruvate accumulated in the system can be isolated and collected by a conventional method. For example, methods such as acidic ether extraction and phenylhydrazonation followed by precipitation isolation can also be employed.
〈実施例〉 以下、実施例によって本発明を説明する。<Example> The present invention will be explained below with reference to Examples.
実施例において生成したピルビン酸の確認と定量は高速
液体クロマトグラフィーによる方法および乳酸脱水素酵
素による方法によって行った。以下の分析結果について
は上記両分析法ともよく合致してあり、同じ分析数値を
示した。Confirmation and quantification of pyruvate produced in the Examples were performed by a method using high performance liquid chromatography and a method using lactate dehydrogenase. The following analytical results were in good agreement with both of the above analytical methods and showed the same analytical values.
実施例１
グルコース１０％、ペプトン１％、Ｋ Ｈ２Ｐｇ／ｆｆ
、ピリドキシン塩酸塩１００μｇ／ｆｌ、ニコチンａ１
００ｔｌ’Ｊ／４１．ｐｌ−１５，０から’；する培地
３０ｍｌ１を５００ ｔｎｉ容振盛フラスコに分注滅菌
後、別に滅菌したＣ ａ Ｃ０３４％を添加し、トルロ
プシス・グラブラータ（ＩＦＯ０００５）を１白金耳植
菌した後、３０’Ｃで９１時間撮隔培養した。ビオチン
は添加しなくともペプトンに含まれる量で十分でおった
。培養後ピルビン酸を定量したところ４０．７３１０．
で市り、残糖は２２３／αであった。副生物は液体クロ
マトグラフィーでは検出されなかった。この培養液１Ｑ
を除菌後、上澄液に塩酸を加え１１２゜０とし、１０．
のエチルエーテルで抽出し、次いで苛性ソーダでｐＨを
６．０に中和した後４０℃で減圧濃縮し１００ｄ程度と
した。この濃縮液にエタノールを滴下させピルビン酸ソ
ーダ２３．１（純度９７％）を得た。Example 1 Glucose 10%, peptone 1%, K H2Pg/ff
, pyridoxine hydrochloride 100μg/fl, nicotine a1
00tl'J/41. After sterilizing and dispensing 30 ml of medium from pl-15,0 to a 500 tni shaking flask, adding separately sterilized Ca C034% and inoculating one platinum loop of Torulopsis glabrata (IFO0005), Culture was carried out at 30'C for 91 hours at intervals. Even without adding biotin, the amount contained in peptone was sufficient. After culturing, pyruvate was quantified and found to be 40.7310.
The remaining sugar was 223/α. No by-products were detected by liquid chromatography. This culture solution 1Q
After sterilization, add hydrochloric acid to the supernatant to bring it to 112°0, and 10.
The extract was extracted with ethyl ether, then neutralized to pH 6.0 with caustic soda, and concentrated under reduced pressure at 40° C. to about 100 d. Ethanol was added dropwise to this concentrated solution to obtain sodium pyruvate 23.1 (purity 97%).
実施例２
実施例１において使用した培地中のグルコースを５％に
減らし、ピリドキシン塩酸塩およびニコチン酸をビオチ
ン０．５μｇ１０．におきかえた以外は同様な培地を用
い、トルロプシス・グラブラータをトルロプシス・メタ
ノロベラセンス（ＡＴＣＣ２６１７６）に変えて実施例
１と同様の方法で培養した。Example 2 Glucose in the medium used in Example 1 was reduced to 5%, pyridoxine hydrochloride and nicotinic acid were added to biotin 0.5 μg 10. The culture was carried out in the same manner as in Example 1, except that the same medium was used, and Torulopsis glabrata was replaced with Torulopsis methanoloverascens (ATCC 26176).
７２時間後、培養液中のピルビン酸を定量したところ２
０．５ｇ／Ｑであり、残糖はなかった。 〈発明の効果
〉
本発明の方法は、発酵法によりピルビン酸を著量蓄積せ
しめることが可能であり、高収率、収量でピルビン酸を
得ることができるとともに、副生物がほとんど生成しな
いため、工業的に極めて有利である。After 72 hours, pyruvic acid in the culture solution was quantified.2
It was 0.5g/Q, and there was no residual sugar. <Effects of the Invention> The method of the present invention makes it possible to accumulate a significant amount of pyruvic acid by fermentation, obtain pyruvic acid in high yield and yield, and generate almost no by-products. It is extremely advantageous industrially.
Claims (3)
、生育のためにチアミンを必要とするピルビン酸生産菌
を培養することにより培地中にピルビン酸を生成蓄積さ
せ、これを採取することを特徴とする発酵法によるピル
ビン酸の製造法。(1) A fermentation method characterized by producing and accumulating pyruvate in a medium by culturing a pyruvate-producing bacterium that belongs to the genus Torulopsis and requires thiamine for growth, and collecting it. Method for producing pyruvic acid by.
ンを必要とする特許請求の範囲第１項記載の発酵法によ
るピルビン酸の製造法。(2) A method for producing pyruvic acid by the fermentation method according to claim 1, wherein the pyruvic acid-producing bacteria further requires biotin for growth.
ｐｓｉｓ）属グラブラータ（ｇｌａｂｒａｔａ）種また
はトルロプシス（Ｔｏｒｕｌｏｐｓｉｓ）属メタノロベ
ッセンス（ｍｅｔｈａｎｏｌｏｖｅｓｃｅｎｓ）種に属
する特許請求の範囲第１項記載の発酵法によるピルビン
酸の製造法。(3) Virupic acid producing bacteria are Torulopsis (Torulo).
A method for producing pyruvic acid by the fermentation method according to claim 1, which belongs to the genus Torulopsis glabrata species or the genus Torulopsis species methanolovescens.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61-42583 | 1986-02-27 | ||
| JP4258386 | 1986-02-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62275688A true JPS62275688A (en) | 1987-11-30 |
| JPH0358275B2 JPH0358275B2 (en) | 1991-09-04 |
Family
ID=12640089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27531086A Granted JPS62275688A (en) | 1986-02-27 | 1986-11-20 | Production of pyruvic acid by fermentation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62275688A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0389620A1 (en) * | 1987-08-21 | 1990-10-03 | Toray Industries, Inc. | Process for preparing pyruvic acid by fermentation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6214789A (en) * | 1985-07-12 | 1987-01-23 | Natl Food Res Inst | Production of pyruvic acid |
| JPS635080A (en) * | 1986-06-25 | 1988-01-11 | バイエル・アクチエンゲゼルシヤフト | 2-trifluoromethyl-benzimidazole |
-
1986
- 1986-11-20 JP JP27531086A patent/JPS62275688A/en active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6214789A (en) * | 1985-07-12 | 1987-01-23 | Natl Food Res Inst | Production of pyruvic acid |
| JPS635080A (en) * | 1986-06-25 | 1988-01-11 | バイエル・アクチエンゲゼルシヤフト | 2-trifluoromethyl-benzimidazole |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0389620A1 (en) * | 1987-08-21 | 1990-10-03 | Toray Industries, Inc. | Process for preparing pyruvic acid by fermentation |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0358275B2 (en) | 1991-09-04 |
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