JPS62258313A - Cosmetic - Google Patents

Abstract

PURPOSE:To provide a cosmetic containing thiamine and/or a thiamine ester as active components, effective in suppressing the aging of skin or formation of skin cancer and preventing suntan, without causing damage or denaturation of the DNA of dermatic cell and having high safety. CONSTITUTION:The objective cosmetic contains thiamine and/or a thiamine ester as active components. Thiamine is vitamin B1 which is a kind of essential vitamin widely distributed in plant, etc. The thiamine ester is e.g. phosphoric acid ester, sulfuric acid ester, etc., preferably a thiamine phosphoric acid ester, especially thiamine pyrophosphate or thiamine monophosphate, etc., existing in living body. Thiamine compound is soluble in water and easily dissolves in face lotion. The amount of the thiamine compound is >=0.1wt%, preferably 0.1-0.2wt%, based on the whole cosmetic.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a cosmetic containing amine and/or thiamin ester and which can be used in sun protection cosmetics, sun protection cosmetics, and the like.

(Prior Art) It is known that sunburn caused by sunlight is caused by ultraviolet rays of 280 to 400 nm. Sunburn is classified into two types. One is acute inflammation accompanied by skin erythema and pain caused mainly by ultraviolet rays in the range 280 to 320 nm (UV rays in the A range), and the other is acute inflammation that is mainly caused by ultraviolet rays in the range 320 to 400 nm (UV rays in the B range or near range). It is a darkening of the skin due to the deposition of melanin pigment caused by ultraviolet rays. 2 for the purpose of tanning the skin to a beautiful tan color without causing skin irritation.
Cosmetics for sun tanning are already manufactured and sold. For example, cosmetics containing ethyl para-aminobenzoate, cinoxate (trade name), oxybenzone (trade name), etc. as UV absorbers (for example, basic cosmetics such as creams and emulsions: foundations).

Finishing cosmetics such as pressed powder) are available.

The ultraviolet absorber used absorbs ultraviolet rays of 280 to 320 nm well and transmits near ultraviolet rays. Therefore,
Although it is possible to darken the skin to the desired degree without causing acute inflammation of the skin, recent studies have revealed that the transmitted near ultraviolet light damages the DNA of skin cells. for example.

It has been reported that thymine dimers are generated in DNA strands by irradiation with ultraviolet light, especially near ultraviolet light. recently,
Instead of determining the degree of sunburn by the SPF value used in the cosmetics field, attempts have been made to determine the degree of sunburn by measuring the thymine dimer using a device. Although it is not known that DNA containing thymine dimers directly causes negative effects on living organisms, the fact that irradiation with ultraviolet rays, especially near ultraviolet rays, causes denaturation of a portion of DNA in skin cells. This is thought to suggest that there is a correlation between the intensity of sunlight and skin blemishes and wrinkles, as well as the occurrence of skin cancer.

Based on this idea, sunlight, especially near ultraviolet rays.

For example, the relationship between Pea and DNA has been studied5.
reported that irradiation with near ultraviolet light in the presence of 4-thiouridine or 4-thiodeoxyuridine, which are substances normally present in cells, causes single-strand breaks in DNA. (J, G.

Peak et al. (1984); Photochemistry and Photobiology (Photoc
hemistry and photobiology)
, 39.713), taking into account that near ultraviolet rays themselves are not absorbed by DNA, it is assumed that near ultraviolet rays do not absorb DNA.
It is thought that the damage in A occurs as a result of a substance capable of absorbing near ultraviolet rays, such as 4-thiouridine and 4-thiodeoxyuridine, absorbing near ultraviolet rays and undergoes some kind of change, which affects the DNA. In particular, oxygen atoms in oxygen-containing compounds in cells are excited by near ultraviolet light, and this is thought to be involved in DNA damage.

In addition to substances that exist in cells, such as the above-mentioned 4-thiouridine and 4-thiodeoxyuridine, substances that are widely present in the environment change when exposed to near ultraviolet irradiation, and this changes the D
It is also possible that it causes damage to NA. For example, nitrosamines, which exist in the air in ppb order,
Alternatively, trace amounts of nitrosamines produced when amines in cosmetics undergo oxidation may affect DNA.

Nitrosamines do not originally have DNA reactivity, but when they change when exposed to near-UV irradiation, they cause mutations in living cells by causing single-strand breaks in DNA or changing the amino acid sequence of DNA. It is known.

In this way, near ultraviolet rays indirectly damage and denature DNA, causing spots and wrinkles on the skin, and eventually skin cancer.

What are the tanning cosmetics currently in use?

Although it can prevent acute skin inflammation, it has no effect on DNA damage caused by absorption of near ultraviolet rays.

(Problem that the invention attempts to solve) The present invention solves the above-mentioned conventional drawbacks.

The purpose is to provide cosmetics that can be applied to sunscreen cosmetics, sunburn prevention cosmetics, etc., and that can suppress DNA damage and, therefore, prevent skin cancer.

(Means and effects for solving the problems) The cosmetic of the present invention contains thiamin and/or thiamin ester, thereby achieving the above object.

Thiamine contained in the cosmetic of the present invention is vitamin B, which is a type of essential vitamin, and is widely contained in plants and the like. As the thiamine ester, phosphoric acid esters, sulfuric acid esters, carboxylic acid esters, etc. are used. Thiamine phosphate esters, particularly thiamine pyrophosphate ester, thiamine monophosphate ester, etc., which exist in living bodies, are suitably used. Cosmetics containing such thiamine and/or thiamine esters (hereinafter referred to as thiamines) may be in any form. For example, basic cosmetics such as lotion, cream, emulsion; foundation, lipstick.

Examples include finishing cosmetics such as pressed powder.

Since thiamines are water-soluble, unlike conventional ultraviolet absorbers 1 such as ethyl para-aminobenzoate, they easily dissolve in lotions. The formulations of these cosmetics are based on the formulations of known cosmetics, and thiamin is added to the total amount of 0.1% of the cosmetics.
It is contained in a proportion of at least 0.1% by weight, preferably 0.1 to 0.2% by weight. If the amount is too low, the effects of thiamin described below will not be obtained.

Even if it is in excess, an effect proportional to the content cannot be obtained. The method for producing the cosmetic of the present invention also follows the usual method. For example, if it's a cream, it's stearic acid.

Oil-based ingredients such as stearyl alcohol, reduced lanolin, and squalene; Emulsifiers such as octyldodecanol, polyoxyethylene cetyl ether, and glycerin monostearate; Water-based ingredients such as water and propylene glycol; Fragrance; Regular cream ingredients such as antioxidants and thiamine are uniformly kneaded using a conventional kneader.

Thiamines contained in the cosmetics of the present invention 1 For example, thiamine has an absorption in the range of 200 to 300 nm as shown in Figure 1 (the figure shows O, a 20mM aqueous sodium phosphate solution containing 1mM thiamine (pH 7.4)). ). Therefore, when the cosmetic of the present invention is applied to the skin, it effectively absorbs mainly A-region ultraviolet rays and does not cause erythema or painful acute inflammation on the skin. Thiamines are
Furthermore, since it does not absorb ultraviolet rays of 300 nm or more, near ultraviolet rays reach the skin without being absorbed and cause melanin pigment deposition. At this time, reaction intermediates from compounds containing excited oxygen and nitrosamines are generated due to near ultraviolet irradiation as described in the "Prior Art" section, but the thiamines contained in the cosmetics of the present invention do not react with these compounds. Has the ability to capture. Therefore, even though near ultraviolet rays reach the skin, the DNA of skin cells is not damaged or denatured. Therefore, when the cosmetic of the present invention containing thiamines is applied to the skin, skin aging and the occurrence of skin cancer can be suppressed.

Since thiamin can transmit near-ultraviolet rays, it can be applied to sun-tanning cosmetics, and if UV absorbers, kaolin, talc, etc., which can also block near-ultraviolet rays are added, it can also be applied to sun-tanning cosmetics. is possible. Thiamine used in the cosmetics of the present invention is vitamin B1, which is a type of essential vitamin.
Thiamine pyrophosphate and thiamine monophosphate are biological products produced as a result of thiamine being ingested and metabolized by the human body. As described above, the thiamines used in the present invention are harmless and beneficial components for the human body.

Therefore, the safety of the cosmetic of the present invention is extremely high.

(Example) The invention will be explained below with reference to examples.

tip top DNA was extracted from phage φ×174 by a conventional method.

This DNA is an RFI type DNA having a double-stranded superhelical circular structure. It is known that when a single strand break occurs in RFI type DNA, it becomes relaxed circular type DNA (RF type II DNA). This RFI type DNA 0.2
0.1 M sodium phosphate buffer containing 15 μg
in μl.

A 4-thiodeoxyuridine solution was added so that the final Q concentration was 0.81. Separately, a cream (emollient cream) was prepared by a conventional method using the formulation shown in Table 1, and its 10+
ng was added to the buffer solution containing 4-thiodeoxyuridine and mixed. This was covered with a 3mm thick transparent glass plate, and the Toshiba Blacklight FL2'0BLB was placed on top of the glass plate.
(20W) Ultraviolet irradiation was performed for 1 hour at room temperature using two tubes. The intensity of ultraviolet rays on the surface of the reaction solution (diameter 7, - circle) containing DNA, 4-thiodeoxyuridine, and cream was 3.5 μ-7 mm.

The reaction solution after UV irradiation was subjected to agarose gel electrophoresis, ethidium staining was performed, and the fluorescence intensity was measured with a densitometer. FI type DNA and RFIT type DNA were each quantified. The results are shown in Table 6. Example 2~
5 and Comparative Examples 1 to 5 are also shown in Table 6. In Table 6, the inhibition rate means that IIFI type DNA is I? It refers to the degree to which it inhibits the development of FII type ON8. Is the inhibition rate 1 when no UV irradiation is performed? The production rate of IIFn type DNA (64%) when using a cream that does not contain thiamine, assuming the production rate of Fn type DNA (14%) as 100%.
Calculations were made assuming Comparative Example 1) as 0%.

Table 1 Cream prescription Next 119l A milky lotion having the formulation shown in Table 2 was prepared by a conventional method.

An experiment was conducted according to Example 1 using 10 ml of this emulsion instead of the cream. However, instead of 4-thiodeoxyuridine (0.8mM), diethylnitrosamine (
1mM> was used.

Table 2: Prescription of Emulsion Powder foundations having the formulations shown in Table 3 were prepared using a conventional method. An experiment was conducted according to Example 1 using 10 ml of this powder foundation instead of the cream. The results are shown in Table 6.

Table 3 Table 4 according to the conventional formula for powder foundation
A lipstick with the formulation shown in was prepared.

An experiment was conducted according to Example 1 using 10 ml of this lipstick instead of the cream. .

Table 4 Lipstick prescription A lotion having the formulation shown in Table 5 was prepared by a conventional method.

Example 1 Using 10w of this lotion instead of cream
The experiment was conducted according to.

(The following is a blank space) Table 5 Formula of lotion A cream was prepared by removing thiamin from the formulation in Table 1 and adding an amount of purified water corresponding to thiamin.

Using this, an experiment was conducted according to Example 1. The results are shown in Table 6.

Calibration 1 A milky lotion was prepared by removing thiamine monophosphate from the formulation in Table 2 and adding purified water in an amount corresponding to the amount of thiamine monophosphate. Use this.

An experiment was conducted according to Example 1. The results are shown in Table 6.

A powder foundation was prepared by removing thiamin from the formulation in Table 3 and adding talc in an amount equivalent to thiamin. Using this, an experiment was conducted according to Example 1. The results are shown in Table 6.

A lipstick was prepared by removing thiamine pyrophosphate from the formulation in Table 4 and adding castor oil, which corresponds to thiamine pyrophosphate. Use this.

An experiment was conducted according to Example 1. The results are shown in Table 6.

Satomongoi A lotion was prepared by removing thiamin from the formulation in Table 5 and adding purified water in an amount equivalent to thiamin. Using this, an experiment was conducted according to Example 1. The results are shown in Table 6.

(The following is a blank space) (Effects of the invention) According to the present invention, ultraviolet rays, especially near ultraviolet rays,
A cosmetic material capable of suppressing DNA tU damage to skin cells caused by this method can be obtained. When using such cosmetics.

The development of sun spots, wrinkles, and even skin cancer is suppressed. The cosmetics themselves are also highly safe. Since this cosmetic absorbs A-region ultraviolet rays and transmits near-ultraviolet rays, it can be suitably used in sun-baked cosmetics, creating ``beautiful tan-colored skin,'' and 1. It does not leave spots or wrinkles. If a UV absorber capable of absorbing A-region ultraviolet rays is added to this cosmetic, it can also be useful as a sunscreen cosmetic.

The figure is a chart showing the UV absorption of thiamine.

that's all

Claims (1)

[Claims] 1. A cosmetic containing thiamine and/or thiamine ester. 2. The cosmetic according to claim 1, wherein the thiamine ester is a thiamine pyrophosphate ester and/or a thiamine monophosphate ester.