JPS62248432A - Culture medium of mushroom - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a culture medium for cultivating edible mushrooms such as oyster mushrooms (artificial shimeji mushrooms), shiitake mushrooms, enoki mushrooms, and nameko mushrooms.

[Conventional technology and its problems]

In recent years, as a method for cultivating edible mushrooms such as enoki mushrooms and oyster mushrooms, bottle cultivation using a culture medium containing a mixture of sawdust and rice bran has become popular, instead of hoda wood cultivation using logs. This method has relatively easy cultivation techniques;
It has the characteristics of a relatively short period from inoculation to harvest, and can be cultivated intensively year-round with simple equipment.

However, recently it has become difficult to obtain sawdust.
It has become necessary to develop alternatives to sawdust.

Therefore, the present invention aims to provide a new culture medium that uses less sawdust, shortens the period from inoculation to harvest, and increases yield.

[Means for solving problems]

In order to solve the above problems, the present invention replaces the sawdust with zeolite in an edible mushroom culture medium made of a mixture of sawdust and rice bran.

[Effect]

Zeolite is a clay mineral that is abundantly produced.

Therefore, it is suitable as a substitute for sawdust, which is difficult to obtain.

Furthermore, the following experiment confirmed that when sawdust was replaced with zeolite, the cultivation speed became faster and the yield increased.

(Experiment 1) A standard culture medium is a mixture of sawdust and rice bran in a 4:1 volume ratio in a test tube, and 30%, 50%, and 100% of the sawdust in this standard culture medium is replaced by zeolite. The effect on vegetative hyphal growth was investigated. The results are shown in Table 1. In addition, the moisture content of the culture medium is 60% in both cases.
After sterilizing the culture medium in an autoclave at 1.2 atm for 30 minutes, the culture medium was cultured in an incubator at 23°C. The amount of hyphal elongation was determined by inoculating the seed fungus onto the top surface of a culture medium packed in a test tube, and measuring the length of downward elongation of the mushroom hyphae after culturing.

Table 1 In this experiment, as shown in Table 1, it was confirmed that by replacing sawdust with zeolite, the elongation of hyphae was promoted and the growth of hyphae was improved.

(Experiment 2) A glass bottle with a volume of 200 ml was filled with 140 g of a culture medium with a moisture content of 60%, sterilized in an autoclave (1.2 atm, 40 minutes), inoculated with inoculum, and incubated at 23°C.
After culturing the mycelium, once the fungi had covered the entire glass bottle, the fungi were covered, watered, and moved to a low temperature of 13°C to wait for fruiting bodies (mushrooms) to form. The results are shown in Table 2.

Table 2 The results in Table 2 show that adding zeolite to the culture medium did not change the yield, but it was found that the number of days required for the cultivation of the bacteria was considerably shortened, and the number of days required for harvesting was shortened.

(Experiment 3) Cultivate in the same manner as Experiment 2! The experiment was carried out by changing the composition of the I group.

The results are shown in Table 3.

Table 3 In this experiment as well, it was revealed that by adding zeolite to the culture medium, the number of days for vegetative mycelial growth (the number of days required for the mycelium to cover the entire bottle) and the number of days for fruiting body maturation were shortened.

〔Effect of the invention〕

Claims (2)

[Claims] (1) A culture medium for edible mushrooms comprising a mixture of sawdust and rice bran, wherein the sawdust is replaced with zeolite. (2) Claim 1 characterized in that 30 to 100% of the sawdust is replaced by zeolite in terms of capacity ratio.
Culture medium for edible mushrooms listed in section.