JPS5847486A - Hypha mass of basidiomycetes or wood for growing mushroom or their preparations - Google Patents

Abstract

PURPOSE:To prepare a wood for growing mushroom to which a hypha mass or mycelium of a germanium-assimilating Basidiomycetes with a high content of germanium is actively attached, by inoculating a seed mold of Basidiomycetes into a mold floor or a raw wood in the presence of a germanium compound, cultivating it under aerated conditions. CONSTITUTION:A seed mold of Basidiomycetes selected from the group consisting of the genus Ganoderma, Elfvingia, Laetiporus, Polyozellus, Lenzites, Inocybe, Fomes fomentarius, Fomitopsis, and Grifola is inoculated into a mold floor consisting of at least sawdust and/or rice bran or a raw wood such as a Japanese beech or a Japanese oak in the presence of an inorganic and/or organic germanium compound such as germanium dioxide, 3-hydroxygermylpropionic acid, etc., cultivated under aerated conditions at about 25-27 deg.C for 40-50 days, and a mycelium of Basidiomycetes is generated and grown while the germanium compound is being assimilated and absorbed.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to fresh water in which mycelial masses or mycelia of germanium-assimilating germanium-rich basidiomycetes belonging to the family Salmonaceae are attached, and to a method for producing the same.

More specifically, the present invention provides germanium-assimilating germanium-rich basidios selected from the group consisting of the genus Stonemanga, the genus Coffitake, the genus Mustatake, the genus Versicolor, the genus Scaleitake, the genus Versicolor, the genus Tsuriganetake, the genus Tsugasanokoshika and the genus Maitake. This invention relates to a mycelia mass or mycelium-attached wood and a method for producing the same.

Basidiomycetes usually reproduce by alternating generations of mycelium, fruiting body, and spores, but it is also possible to reproduce only mycelium.
Since it is difficult to generate and propagate eH fruiting bodies industrially, mycelium is propagated industrially. In recent years, it has been discovered that polysaccharides extracted from a certain basidiomycete of the family Salmonaceae have medicinal efficacy as an anticancer drug. Since there are great expectations for its medicinal efficacy, it is eagerly awaited to establish a method for propagating its mycelium and producing large quantities of its fruiting bodies.

By the way, among the basidiomycetes, the basidiomycetes belonging to the genus Stonebush, in particular the TLH extract produced from the basidiomycetes, act as an immunostimulant and increase resistance to foreign substances such as cancer that occur in the human body. The results of clinical trials have confirmed that it suppresses the proliferation of cancer cells, removes cancer cells, and more preferably has cholesterol-lowering and blood pressure-lowering effects, and has therapeutic effects on chronic hepatitis, rheumatism, etc. has been done.

Furthermore, germanium compounds, especially organic germanium compounds, have attracted attention because they have important therapeutic effects such as anticancer effects. It has been found that germanium has the effect of promoting the growth of fungal bodies and fruiting bodies.

Therefore, the present inventors biosynthesized germanium compounds and fungal body components such as glycoproteins and polyphytes contained in basidiomycete seedlings belonging to the family Salmonaceae, and synthesized them within the mycelium and/or fruiting body of the basidiomycete. For the purpose of synergizing the medicinal efficacy of both the germanium compound and the germanium compound, and to discover new medicinal efficacy by converting it into an unknown organogermanium compound, for example, germanium compounds are added to the culture medium of Manga chinensis. By assimilating and absorbing the mycelium and fruiting body, it is possible to obtain the mycelium and fruiting body of Cinnamon mushroom, which has excellent medicinal properties, and the addition of organic germanium compounds has a significant effect on the development and growth of the mycelium and fruiting body. The present inventors have discovered that the method is effective and that it is possible to produce a large amount of mycelium and fruiting bodies in a shorter period of time compared to conventional culture and cultivation methods, and have completed the present invention.

Next, the present invention will be explained in detail.

The present invention involves inoculating a fungus bed of at least sawdust and/or rice bran or logs in which an inorganic and/or organic germanium compound is present with an inoculum of a basidiomycete belonging to the family Salmonaceae, and culturing it under aerated conditions. 16 clusters or mycelia of germanium-assimilating germanium-rich basidiomycetes belonging to the family Salmonaceae, which are obtained by generating and multiplying the mycelia of the basidiomycetes while assimilating and absorbing the germanium compounds. Citrus cypress trees and their production methods.

Specific examples of the basidiomycetes used in the present invention include Cinnamon edulis, Cinnamon chinensis (Linocula spp.), Coffixarnum edulis, Mustard edulis, Versicolor versicolor, Scale edulis, Betula edulis, Turiga edulis, Tsugasan erectum, Chimireimaitake, and the like. The seed culture medium, fungal bed, and inoculation medium used in the present invention include (1) sawdust from cypress, beech, oak, etc., (2) rice bran, defatted rice bran, (3) water,
(t) Composed of slaked lime, etc. If a small amount of a mixture of edible oils and fats and edible emulsifiers, QjllWs such as glucose, pheasant rose, and mannose, vitamins such as piotin and vitamin B7, and amino acids such as glutamate and sodium aspartate are added to the medium, the mycelium and offspring of basidiomycetes can be stimulated. The occurrence and growth of the fruit is further promoted, and a mycelial mass or tank water with high germination activity can be obtained. The logs used in the present invention are beech trees, oak trees, etc. These log demons, for example, have a diameter of about 10 ct.
n, length approximately /jan, but is not limited to these.

As a container for inoculation, it is easy to use something that is durable and flexible, such as stainless steel. The size of the inoculation container is not particularly limited, but for example, a nylon bag (a bag shaped like a barb, with an MM upper part and a sealed lower part).
In this case, one with a length of about 20 cm and a width of about 1 cm is used. As the metal can, a box-like object with an opening at the top and a bottom, a box-like object, etc. are used.

Place the container in the container and pour water to an extent that does not exceed the top surface of this S stand to create a water storage section. The water in the water storage section becomes steam in the subsequent heat sterilization and cultivation steps, and an appropriate amount of moisture is constantly supplied to the fungal bed, raw wood, mycelium, etc. in the inoculation container in an aseptic manner.

When a mycelial mass is obtained from a fungal bed and cultivated in the same container, moisture is similarly supplied to the mycelial mass and fruiting body. In order to facilitate the evaporation of water vapor, it is desirable to provide a large number of through holes on the top surface of the F2 mounting base.

i+'
Inorganic germanium is impregnated into a fungal bed or raw wood, specific examples of which include: inorganic germanium such as germanium oxide, germanium chloride, 3-hydroxygermyl, propionate, organic germanium polymers, etc. Examples include organic germanium. As the organic germanium polymer, for example, a low molecular weight 3-oxygermylpropionic acid polymer, a high molecular weight 3-oxygermylpropionic acid polymer, etc. are used. If the inorganic germanium compound is used in combination with citrate, polyphosphate, etc., the amount assimilated and absorbed into the mycelium and fruiting body of the basidiomycete described above will further increase. It is preferable to use an organic germanium compound because it has the above-mentioned growth promoting effect on the basidiomycete and is easily assimilated and absorbed by the bacterial cells.

In order to impregnate a germanium compound marked R into a solid medium such as a fungus bed or raw wood, (1) add the germanium compound to the water in the water storage part, impregnate or apply the mixture, (4') add the germanium compound to the water in the water storage part, and (4') add the germanium compound to the water in the water storage part. When pouring a top inoculation medium to facilitate inoculation, add the germanium compound to this inoculation medium; (5) make 7 or 2 holes on the cut surface φ of the log or its circumference; Fill the hole with a carrier such as cotton, cloth, or paper sponge impregnated with a germanium compound, a germanium compound coated with starch or the like, or a germanium compound made of a poorly soluble germanium compound. etc. will be carried out. According to method (s), germanium compounds are gradually supplied to the fruiting body over a long period of time and are assimilated into the fruiting body, as it is gradually diffused throughout the log each time water is sprayed in the subsequent fruiting body production process. Entities can be highly germanized.

The concentration of an inorganic germanium compound in a medium is, for example, about 100 to 1,000 ppM, and a low concentration of 1100 PP or less is not very effective in assimilating and absorbing germanium compounds. At high concentrations, it adversely affects the growth and growth of mycelium and fruiting bodies. Organogermanium compounds have extremely good assimilation and absorption, and even at a certain high concentration (l
Toe /3 (1) OppM) Does not have any adverse effect on the development, proliferation, growth, etc. of mycelium and fruiting bodies.

8! Place the S stand in the container for bacteria, place the fungus bed or raw wood on this place, pour the inoculation medium as necessary, close the upper opening of this container, and place it in a steam heating oven. Store and heat sterilize. In normal pressure sterilization, it is steamed for about 10 to 2 hours at the 10th point, and in high pressure sterilization, it is steamed at /10 to /20'C for about to minutes. After turning off the heat, set up a sterile filter, culture under aerated conditions, and inoculate the fungus bed or raw wood. When cultured at about 23° to 27 aC for 10 to 10 days, mycelium will develop and proliferate, and a mycelial mass or mycelium will take root and a rotten tree will be obtained. A small amount of water vapor is constantly supplied from the water storage part at the bottom of the inoculation container during the cultivation period, so the inside of the inoculation container is maintained aseptically and at the maximum humidity, and the mycelium, medium, etc. Since an appropriate amount of moisture is supplied and the oxygen content is increased, the conditions are optimal for the generation, proliferation, and growth of mycelium.

The mycelial mass obtained using the container cultivation method is kept at a humidity of 60% in the cultivation room.
~90%, and grown at a temperature of 25-30'C under well-ventilated conditions, avoiding direct sunlight and in bright light, fruiting bodies can be harvested in about 40 days.

In the log cultivation method, flat land with good drainage is grown at a depth of 1Qc.
The excavated soil was excavated (the capital was buried leaving the upper part) and the upper part was covered with small gravel and porous earth and sand such as Kanuma-jo.After that, a vinyl house was built and the temperature inside the house was set to 30-30℃.
3 Maintain czm degree at approximately 0%. Cultivate by avoiding direct sunlight and avoiding drying. After surrender/1
Germination occurs in 1 day 7-j, fruiting bodies are formed in 5 o-sr days, and the seeds can be harvested. In this case, the degree of tears is tO~50%, and the temperature is 2g~3. ! 'Keep at C.

The above descriptions are mainly about the mandarin mushroom, but as raw wood, for example, the wood of the coffin mushroom is beech, oaks, chestnuts, etc., the vermilion mushroom is a broad-leaved tree, the chimney mushroom is a coniferous tree such as hemlock, the scale mushroom is a broad-leaved tree, and the chili mushroom is a broad-leaved tree. Beech trees are used for this, conifers are used for Aikawaratake, and Quercus oak and beech are used for Masufuke.

The cultivation method and cultivation method of these basidiomycetes may be carried out in accordance with the cultivation method and cultivation method of Cinnamon mushroom.

Example Inside a U drawing container containing a mounting table with a bottomed water-resistant, heat-resistant, and flexible synthetic resin bag (heat-resistant nylon) with the top open and a large number of through holes drilled on the top surface. Add % sodium polyphosphate aqueous solution/liter to an extent that does not exceed the top surface of this mounting table, and use the lower part of the mounting table as a water storage section. On this mounting table, a diameter of approximately /Qcm,
Place 70 rounded logs of length /j; tab in a vertical line, close the top opening of the synthetic resin bag, heat sterilize it in an autoclave at /20'C for 30 minutes, and store it in the Taisho period. Pour the 2-hour retainer onto the cut surface of the log and open the opening a.
5 gates are provided, and the inoculum is massaged from the outside of the bag to spread the inoculum over the entire cut surface of the log, and then cultured for 3 days at 2! -27'C under aerated conditions.

In 30 days, you can get a water sample with white mycelium spread all over the log.

The harvested cypress trees are buried in the soil at regular intervals, leaving the tops intact, and the tops are covered with small gravel and Kanuma soil.

Build a vinyl house on top of that and keep the temperature inside the house at 30.
Maintain ~33 °CNW degrees at lrO%. Avoid drying by watering and avoid direct sunlight. The seeds germinated in 7.2~/day after being laid down, and then the temperature was increased to 2ff~32°C1 humidity '10-
! ;096 for better fruiting body development. Fruiting bodies grow in So-5g days after lying down, and are harvested. Three to II fruiting bodies were hatched from seven logs, and the length of their stems was approximately Qcm, and the diameter of the cap was approximately QC.
1l, the underside of the umbrella is yellow. The fruiting body obtained by inoculating, culturing, and cultivating under the same conditions except that no germanium compound was used has a stem length of about 10 CwS and a diameter of about 6 cm, and the underside of the cap is white. There are 1 to 3 fruiting bodies per 7 logs, with an average of 2 fruiting bodies, and because the size of the harvested fruit is uneven and there are some with inferior commercial value, a process of sorting the fruiting bodies is necessary. There are also disadvantages.

On the other hand, when germanium compounds are used,
All of the fruiting bodies that can be harvested have a cap diameter of about 77 cm or more, and the product price is very high because the cap thickness is very thick, and there is no need for a sorting process for the harvested fruiting bodies.

As is clear from the above explanation, the fruiting body of the basidiomycete of the present invention is large and thick, and has high commercial value, the yield of fruiting body per 7 logs is high, and the fruiting body of the basidiomycete of the present invention is large, and the fruiting body of the basidiomycete of the present invention is large, and the yield of fruiting body per 7 logs is high. It has various characteristics, such as the fact that it contains a large amount of complex compounds that are biosynthesized and combined with germanium compounds and bacterial body components such as proteins and polysaccharides contained in bacteria. The fruiting body of the present invention is clearly distinguished from the conventional fruiting body in that the underside of the cap is yellow, which indicates that the content of germanium components in the fruiting body is extremely high. The content of germanium components can be clearly identified by the degree of shading of the yellow-white color on the back of the cap. As mentioned above, when no germanium compound is used, the underside of the fruit body obtained is white.

The germanium component content of the mycelium of the basidiomycete of the present invention is 1
1000 ppM or more, and the fruiting body obtained by cultivating using the mycelium mass of the present invention or the tank water in which the mycelium is attached, for example, the fruiting body of the stone mushroom, has a germanium component content of about 1500 ppM, and the underside of the cap has a germanium component content of about 1500 ppM. They are yellow in color and contain a large amount of germanium.

In addition, the fruiting body obtained without using a germanium compound, for example, the content of germanium precomponents in the fruiting body of Cinnamon edulis is 1OO~700 PPM, and the fruiting body of Cinnamon edulis cultivated in a cold region has a germanium content of 700 ppm.
pbi, the content of those grown in lowlands is ≠00 PPM
The inventor has found that. It is believed that the reason why it has been empirically said that the Cinderaceae mushrooms, which grow naturally in cold regions, have high medicinal efficacy is due to the high content of this germanium component. It is presumed that the germanium compounds assimilated and absorbed by basidiomycetes such as C. chinensis exist in the bacterial cells as complexes such as complex salts with glycoproteins, polysaccharides, etc. in the bacterial cells.

Claims (1)

[Scope of Claims] / A fungal bed or log consisting of at least sawdust and/or rice bran in the presence of an inorganic and/or organic germanium compound. An inoculum of a basidiomycete selected from the group consisting of the genus Coffitake, the genus Mustatake, the genus Versicolor, the genus Scale, the genus Citrus, the genus Tsuriganetake, the genus Tsugasanokoshika and the genus Maitake is inoculated and cultured under aerated conditions to produce the germanium compound. Generate mycelium of the basidiomycete while assimilating and absorbing it,
A water test in which a mycelial mass or mycelium of germanium-assimilating germanium-rich basidiomycetes, which is obtained by growing germanium, has taken root. 2. A fungal bed or log made of at least sawdust and/or rice bran in which an inorganic and/or organic germanium compound is present is used to incubate a bed of fungi or logs made of at least sawdust and/or rice bran. The invention is characterized by inoculating a basidiomycete seed fungus selected from the group consisting of the genus Salmonella and the genus Maitake, and culturing it under aerated conditions to assimilate and absorb the germanium compound while generating and growing the mycelium of the basidiomycete. A method for producing sample water in which mycelial masses or mycelia of germanium-assimilating germanium-rich basidiomycetes have taken root. 3. In an inoculation container with an opening at the top, a mounting table with a large number of through holes on the top surface is housed, and an inorganic and/or organic germanium compound is added to the extent that it does not exceed the top surface of the mounting table. Place the fungus bed or raw wood made of at least sawdust and/or rice bran on the mounting table, close the opening, heat sterilize it in a heating oven, and leave it to cool under sterile conditions. Claim 2, characterized in that a downwardly closed opening is opened, a basidiomycete inoculum is inoculated through the opening, a filter is provided in the opening, and the culture is carried out under aeration conditions. A method for producing ice samples on which mycelial masses or mycelia of germanium-assimilating germanium-rich basidiomycetes are attached. In a container for inoculation with an opening at the top, a mounting table with a number of through holes on the top surface is housed, and water is injected to the extent that it does not exceed the top surface of the mounting table, and at least sawdust and/or
Alternatively, place a fungal bed or log made of rice bran on a mounting table, pour a mixture of inorganic and/or organic germanium compounds into an inoculation medium onto the fungal bed or log, close the opening, and heat. Sterilize by heating in a pot, leave to cool, open the closed opening under aseptic conditions, pour and inoculate the basidiomycete inoculum through the opening, place a filter in the opening, and culture under aeration conditions. The second claim characterized in that
A method for producing an ice test on which a mycelial mass or mycelium of germanium-assimilating germanium-rich basidiomycetes as described in 2. S. Production of germanium-assimilating germanium-rich mycelial mass or mycelia-attached mycelia according to claim 3 or 3, wherein the inoculation container is a metal can. Law. g. A method for producing a germanium-assimilating germanium-rich mycelium mass or a tree on which mycelium has taken root, characterized in that the inoculation container is a synthetic resin bag.