JPS6221502B2 - - Google Patents

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Publication number
JPS6221502B2
JPS6221502B2 JP59033338A JP3333884A JPS6221502B2 JP S6221502 B2 JPS6221502 B2 JP S6221502B2 JP 59033338 A JP59033338 A JP 59033338A JP 3333884 A JP3333884 A JP 3333884A JP S6221502 B2 JPS6221502 B2 JP S6221502B2
Authority
JP
Japan
Prior art keywords
extract
seafood
treatment
solution
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP59033338A
Other languages
Japanese (ja)
Other versions
JPS60180566A (en
Inventor
Akyoshi Yamane
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP59033338A priority Critical patent/JPS60180566A/en
Publication of JPS60180566A publication Critical patent/JPS60180566A/en
Publication of JPS6221502B2 publication Critical patent/JPS6221502B2/ja
Granted legal-status Critical Current

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  • Meat, Egg Or Seafood Products (AREA)

Description

【発明の詳細な説明】[Detailed description of the invention]

本発明は、魚介類のエキス剤の製造法に関す
る。 従来、ウナギやエビをアルコール含有飲料に浸
漬処理することによる栄養酒を製造する方法は公
知であり、コイを糖含有アルコール飲料に浸漬し
て加熱処理することにより栄養飲料を製造する方
法も公知である。また、魚介類エキスの抽出にお
いて、加熱抽出処理や高温下での分解処理を行う
場合油脂の酸化やメラード反応などの化学変化を
起し易く、色調、香、味覚ともに著るしく劣化し
た製品を生ずるおそれがあるのである。 本発明者は、0℃以下においても酵素が充分に
その分解作用を有することを知見し、アミノ酸そ
の他有効成分を多量に含有すると共に、色、香、
味の良好な魚介類のエキス剤を得る方法について
研究した結果、本発明を達成したのである。 本発明は、油脂の変敗や色、香の劣化を来たす
ことなく、魚介類から旨味成分その他の有効成分
を多量に含有するエキス剤を製造する方法を提供
することを目的とする。 本発明は、魚介類を、酵素を添加した、食塩、
しよ糖、またはエチルアルコールの単独溶液もし
くは混合溶液に浸漬し、0℃〜−15℃の温度の範
囲内の温度下で、凍らない状態において、抽出処
理した後、加熱処理し酵素を失活させた後、固形
分・油脂分を分別処理することを特徴とする魚介
類の抽出液を製造する方法である。 本発明での魚介類とは、コイ、エビ、ウナギ、
ハマグリその他の淡水産、海水産の魚類、貝類を
意味し、それらの形状により、たとえば小型のも
のはそのままの姿、または内臓等を除去した姿、
大型のものは内臓等を除去し、または除去せずし
て、ブツ切りまたはミンチがけ等により適宜の大
きさになした姿等適宜所望形態のものが使用され
る。浸漬液として使用する食塩、しよ糖、または
エチルアルコールの単独溶液もしくは混合溶液と
は、0℃〜−15℃の温度範囲下で未凍結状態の魚
介類の浸漬処理において、魚介類から有効成分を
抽液乃至浸出することができ、かつ添加された酵
素を不活性化することが無い濃度を有する水溶液
を意味し、食塩、しよ糖、またはエチルアルコー
ルその他氷点降下性能を有し、飲食用に供される
ものの単独溶液または混合溶液を意味する。たと
えば、混合溶液の場合、エチルアルコール15%溶
液3〜4にはしよ糖500〜600gを混和した溶液
が使用され得る。また、浸漬処理での浸漬液の使
用量は、使用魚介類の2〜3倍量が好ましい。た
とえば、コイ1.5Kg程度に3.0〜4.5程度使用され
得る。また本発明で酵素を添加するのは、0℃以
下の低温で浸漬処理するので、自己消化酵素だけ
では分解が不十分なので、これを補うために酵素
添加するのであり、使用酵素としては、主として
蛋白分解酵素であつて、味、香の良いエキスを得
るには、アルカリ性・中性プロテアーゼが好まし
く、使用魚介類当りの使用量は5万〜10万単位で
0.1〜1.0%が好ましい。浸漬処理時間は、長時間
を要し、たとえば、−3℃では5〜14日間を要
し、より低温度の場合には、延長し、より高音の
場合には短縮することができ、所望時間浸漬(抽
出)処理するのである。さらに、加熱処理とは、
添加した蛋白分解酵素などを失活させる程度の温
度で短時間加熱することを意味し、未分解蛋白を
凝固させることが好ましい。 本発明により得られる抽出液は、そのままもし
くは適宜濃度に濃縮し、または呈味成分、さらに
栄養成分をも配合して飲用等に供される。また、
上記液状物を粉末化または賦形剤などを混和して
顆粒その他の固形化とすることができる。 本発明によれば、食塩、しよ糖、またはエチル
アルコールなどの氷点降下性能をもつと共に飲食
用に供し得るものの、単独溶液または混合溶液を
使用し、0℃〜−15℃の温度範囲下で浸漬処理す
るので、上記氷点降下性液の魚介類への浸透など
により魚介類を凍らせることなく、また魚介類の
腐敗防止(細菌、低温細菌糖の微生物の抑制、揮
発性塩基窒素の抑制)や油脂の変敗の防止などを
して浸漬(抽出)処理ができると共に、味に関与
するアミノ態窒素が徐々に増加するのが認められ
るばかりでなく、上記抽出液(浸漬液)に蛋白分
解酵素が添加されているので、グルタミン酸、グ
リシン、アルギニンその他のアミノ酸が高濃度に
含有され、しかも抽出処理で加熱手段を用いない
ので色調も良好な抽出液が得られるのである。 次に、本発明の実施態様を記載する。 実施例 1 ブツ切りにした黒コイ1.5Kgを、15%アルコー
ル溶液4にしよ糖600gを溶解し、さらに蛋白
分解酵素(市販のタシナーゼN−11−100を使用
した)を0.1%添加した、−3℃の抽出液に6日間
浸漬した。次いで、約60℃で短時間加熱して酵素
を失活させると共に未分解の蛋白質を凝固させた
後、固形分を分別し、油分を分離して得た液を濃
縮処理して濃縮抽出液を得た。 本実施例1記載と同様資材を使用して、抽出処
理において、約80℃で約8時間の加熱処理によつ
て、加熱抽出と濃縮を行つた後、固形分・油分を
分別して濃縮抽出液を得た。 氷温抽出による本実施例1(試験区)と加熱抽
出による上記方法(対照区)との濃縮抽出液を比
較した結果は次のとおりである。
The present invention relates to a method for producing a seafood extract. Conventionally, a method of producing a nutritional drink by immersing eel or shrimp in an alcohol-containing beverage is known, and a method of producing a nutritional drink by immersing carp in a sugar-containing alcoholic beverage and heat-treating the same is also known. be. In addition, when extracting seafood extracts, heat extraction treatment or decomposition treatment at high temperatures tends to cause chemical changes such as oxidation of fats and oils and Mellard reaction, resulting in products with significantly deteriorated color, aroma, and taste. There is a possibility that this may occur. The present inventor found that enzymes have a sufficient decomposition effect even at temperatures below 0°C, and in addition to containing large amounts of amino acids and other active ingredients,
The present invention was achieved as a result of research into methods for obtaining seafood extracts with good taste. An object of the present invention is to provide a method for producing an extract containing a large amount of flavor components and other active ingredients from seafood without causing deterioration of fats and oils and deterioration of color and aroma. The present invention provides seafood with enzyme-added salt,
The enzyme is immersed in a single or mixed solution of sucrose or ethyl alcohol, extracted at a temperature between 0°C and -15°C without freezing, and then heated to deactivate the enzyme. This is a method for producing a seafood extract, which is characterized by separating the solid content and oil and fat content. Seafood in the present invention includes carp, shrimp, eel,
Refers to clams and other freshwater and saltwater fish and shellfish, depending on their shape, such as small ones as they are, or with their internal organs removed,
Large ones are used in any desired shape, such as by cutting or mincing them into appropriate sizes with or without removing internal organs, etc. A single solution or a mixed solution of table salt, sucrose, or ethyl alcohol used as a dipping liquid is used to soak unfrozen seafood in a temperature range of 0°C to -15°C to remove active ingredients from seafood. It means an aqueous solution that can extract or leach the enzyme and has a concentration that does not inactivate the added enzyme, and that has the ability to lower the freezing point of salt, sucrose, or ethyl alcohol, and is edible. means a single solution or a mixed solution of the substances to be subjected to. For example, in the case of a mixed solution, a solution in which 500 to 600 g of sucrose may be mixed with 15% ethyl alcohol solution 3 to 4 may be used. Further, the amount of dipping liquid used in the dipping treatment is preferably 2 to 3 times the amount of the seafood used. For example, about 3.0 to 4.5 can be used for about 1.5 kg of carp. In addition, enzymes are added in the present invention because the immersion treatment is carried out at a low temperature of 0°C or lower, so the decomposition is insufficient with autolytic enzymes alone, so enzymes are added to compensate for this. Among proteolytic enzymes, alkaline and neutral proteases are preferred in order to obtain extracts with good taste and aroma, and the amount used is 50,000 to 100,000 units per seafood used.
0.1-1.0% is preferred. The soaking treatment time takes a long time, for example 5 to 14 days at -3°C, and can be extended for lower temperatures and shortened for higher temperatures, depending on the desired time. It is immersed (extracted). Furthermore, heat treatment is
This means heating for a short time at a temperature that deactivates added proteolytic enzymes, etc., and preferably coagulates undegraded proteins. The extract obtained by the present invention can be used for drinking as it is, concentrated to an appropriate concentration, or mixed with taste components and nutritional components. Also,
The above liquid material can be powdered or mixed with excipients to form granules or other solids. According to the present invention, a single solution or a mixed solution of salt, sucrose, or ethyl alcohol, which has freezing point lowering properties and can be used for consumption, is used in a temperature range of 0°C to -15°C. Since the immersion process is performed, the seafood does not freeze due to penetration of the above-mentioned freezing point-depressing liquid into the seafood, and prevents spoilage of the seafood (suppression of microorganisms such as bacteria and low-temperature bacteria sugars, and suppression of volatile base nitrogen). Not only can the soaking (extraction) treatment prevent the deterioration of fats and oils, but also the gradual increase in amino nitrogen, which is involved in flavor, has been shown to improve the ability of the extract (soaking liquid) to undergo protein decomposition. Since enzymes are added, the extract contains glutamic acid, glycine, arginine, and other amino acids in high concentrations, and since no heating means are used in the extraction process, an extract with good color can be obtained. Next, embodiments of the invention will be described. Example 1 1.5 kg of cut black carp was dissolved in 15% alcohol solution 4 with 600 g of sugar, and 0.1% of proteolytic enzyme (commercially available Tacinase N-11-100 was used) was added. It was immersed in an extract solution at 3°C for 6 days. Next, after heating at approximately 60°C for a short time to inactivate the enzyme and coagulate undegraded proteins, the solid content is separated and the oil content is separated.The resulting liquid is concentrated to obtain a concentrated extract. Obtained. Using the same materials as described in Example 1, in the extraction process, heat extraction and concentration were performed by heat treatment at about 80°C for about 8 hours, and then the solid content and oil content were separated and the concentrated extract was obtained. I got it. The results of comparing the concentrated extracts of this Example 1 (test group) using ice-cold extraction and the above method (control group) using heated extraction are as follows.

【表】【table】

【表】【table】

【表】 上記のように、試験区(実施例1)の抽出液中
には、対照区の抽出液中のアミノ酸よりも全般的
に多量に含有され、3倍以上となつている。な
お、試験区の抽出液には、対照区の抽出液より
も、蛋白質、炭水化物が多、また脂肪が少なく含
有されていた。 実施例 2 ブツ切りにした黒コイ1.5Kgを、食塩60Kgを4
の水に溶解し、さらに蛋白分解酵素(市販のタ
シナーゼN−11−100)0.1%添加した、−0.5℃の
抽出液に15日間浸漬した。 次いで、実施例1と同様に、酵素失活処理する
と共に未分解蛋白質凝固処理した後、分別処理
し、濃縮処理して濃縮抽出液を得た。 実施例 3 30%アルコール溶液4に、蛋白質分解酵素
0.1%添加して抽出液を得、該抽出液に黒コイの
ブツきり1.5Kgを入れ、−12℃で15日間浸漬処理し
た。 次いで、実施例1と同様に、酵素失活処理と共
蛋白質凝固処理し、分別処理に濃縮処理して、濃
縮抽出液を得た。 実施例 4 黒コイのブツ切れ1.5Kgを、実施例1と同様の
抽出液に入れ、−8℃の温度下で15日間抽出処理
した。次いで約60゜で短時間加熱処理しつ、酵素
を失活させると共未分解の蛋白質を凝固させた
後、固型分を分離し、凍結して濃縮液を得た。 上記各実施例のパネルによる官能的試験を表3
に示し、遊離アミノ酸分析結果を表4に示す。
[Table] As described above, the extract of the test group (Example 1) generally contained a larger amount of amino acids than the extract of the control group, more than three times as much. The extract from the test group contained more protein and carbohydrates, and less fat than the extract from the control group. Example 2 1.5 kg of black carp cut into pieces and 60 kg of table salt
The sample was dissolved in water and immersed for 15 days in an extract solution at -0.5°C to which 0.1% of proteolytic enzyme (commercially available Tacinase N-11-100) was added. Next, in the same manner as in Example 1, the mixture was subjected to enzyme deactivation treatment and undegraded protein coagulation treatment, followed by fractionation treatment and concentration treatment to obtain a concentrated extract. Example 3 Add proteolytic enzyme to 30% alcohol solution 4
0.1% was added to obtain an extract, and 1.5 kg of black carp pieces were added to the extract and immersed at -12°C for 15 days. Next, in the same manner as in Example 1, enzyme inactivation treatment and coprotein coagulation treatment were performed, followed by fractionation treatment and concentration treatment to obtain a concentrated extract. Example 4 1.5 kg of pieces of black carp were placed in the same extraction solution as in Example 1, and extracted for 15 days at a temperature of -8°C. Next, the mixture was heated at approximately 60° for a short time to inactivate the enzyme and coagulate undegraded proteins, and then the solid content was separated and frozen to obtain a concentrated solution. Table 3 shows the panel sensory test for each of the above examples.
The results of free amino acid analysis are shown in Table 4.

【表】【table】

【表】【table】

【表】 上記対照区は、熱水抽出を行い、分離、濃縮処
理を行つて得た濃縮抽出液である。
[Table] The above control group is a concentrated extract obtained by performing hot water extraction, separation, and concentration treatment.

Claims (1)

【特許請求の範囲】[Claims] 1 魚介類を、酵素を添加した、食塩、しよ糖、
またはエチルアルコールの単独溶液もしくは混合
溶液に浸漬し、0℃〜−15℃の温度範囲内の温度
下で凍結されない状態において抽出処理した後、
加熱処理して酵素と不活性化してから固形分と油
分とを分別処理することを特徴とする魚介類から
抽出液を製造する方法。
1 Seafood with added enzymes, salt, sucrose,
Or, after being immersed in a single solution or a mixed solution of ethyl alcohol and subjected to extraction treatment in an unfrozen state at a temperature within the temperature range of 0°C to -15°C,
A method for producing an extract from seafood, which comprises heating to inactivate enzymes and then separating solids and oils.
JP59033338A 1984-02-25 1984-02-25 Preparation of extracted solution from fish and shellfish Granted JPS60180566A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP59033338A JPS60180566A (en) 1984-02-25 1984-02-25 Preparation of extracted solution from fish and shellfish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59033338A JPS60180566A (en) 1984-02-25 1984-02-25 Preparation of extracted solution from fish and shellfish

Publications (2)

Publication Number Publication Date
JPS60180566A JPS60180566A (en) 1985-09-14
JPS6221502B2 true JPS6221502B2 (en) 1987-05-13

Family

ID=12383777

Family Applications (1)

Application Number Title Priority Date Filing Date
JP59033338A Granted JPS60180566A (en) 1984-02-25 1984-02-25 Preparation of extracted solution from fish and shellfish

Country Status (1)

Country Link
JP (1) JPS60180566A (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427454A (en) * 1987-07-22 1989-01-30 Mizusawa Industrial Chem Concentrated shellfishes and production thereof
JPH01285176A (en) * 1988-05-11 1989-11-16 Hideto Eguchi Healthy food
JPH089923A (en) * 1994-06-29 1996-01-16 Ajinomoto Co Inc Production of extract for food

Also Published As

Publication number Publication date
JPS60180566A (en) 1985-09-14

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