JPS62160103A - Filter separation device - Google Patents

Abstract

PURPOSE:To reduce cloggings of filter medium, minimize danger of contamination of filtrate and carry out filtering of suspension for a long period of time by revolving a rotor close to the filter medium on raw liquid side during filtering of raw liquid. CONSTITUTION:A filtrate chamber 14 consisting of a ceramic membrane 12 and a substrate 13 for the membrane is formed on part of the wall of a culture tank 11. A magnet rotor 15 is installed revolvably by a rotor substrate 16 on the opposite side of the filtrate chamber 14 through the membrane 12. The said rotor substrate 16 is preferably made of a network material. A filtrate thief line 17 is installed to the filtrate chamber 14, being connected with a pump 18. A rotor working body 19 made of magnet is installed facing to the rotor 15 through the membrane 12 and the filtrate chamber 14. When variation of concentration in the culture tank 11 is measured, the sample is drawn by generating turbulence on the surface of membrane 12 by means of revolution of the rotor 15, enlarging shearing speed for the wall and filtering by means of negative pressure generated with the pump 18.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a filtration separation device. More specifically, it is used in various industrial fields including the food and pharmaceutical industries, where various high molecular weight substances are used as solutes, and these suspended components are separated from suspensions containing microorganisms, cells, and other fine particles. The present invention relates to a filtration separation device.

[Prior art] In the food and pharmaceutical industries, low molecular weight substances produced in reaction tanks such as fermenters and culture tanks are combined with formed components such as microorganisms, cultured cells, unreacted solids, and foreign substances. separation is taking place. Conversely, when obtaining microorganisms and cells as products, separation is performed for the purpose of separating them from the culture medium and removing waste products that inhibit their growth. Methods for separating each particle component from such a suspension, that is, a suspension containing a high molecular weight substance with a molecular weight of 1,000 to 2,110,000,110 as a solute and further containing particles with a size of 1 to 1,001 μm, include centrifugation and deep layer separation. Filtration, precision filtration, ultrafiltration, etc. are mainly used.4 The depth filtration method uses a thick fibrous sheet or mat made of asbestos, filter paper, glass fiber, etc. as a filter medium, and 7 Precision filtration and ultrafiltration are surface filtration methods in which the removal is mainly carried out on the membrane surface, allowing for more accurate separation compared to depth filtration. can.

[Problems to be solved by the invention] Centrifugal separation is a commonly used method for clarifying such suspensions; There were problems such as the incorporation was cumbersome and the process was susceptible to contamination during separation.

In addition, in the case of deep filtration, precision filtration, and ultrafiltration methods that use filter media, the 9 surfaces of the filtration tube are easily clogged due to suspension components in the suspension, and when filtration is performed for a long time, Because it required a process of backwashing and other cleaning to eliminate clogging, it could not be used continuously.2 In particular, it was difficult to prevent changes in the concentration of products and substrates in reaction tanks such as fermenters and culture tanks. When monitoring, products and substrates are either filtered by microfiltration or the like, or vertical filtration quickly clogs and cannot be used continuously for long periods of time. In addition, with conventional cross-flow filtration methods, clogging is unlikely to occur, and there is a high risk of contaminating the inside of reaction tanks such as fermenters and culture tanks due to damage to pumps and circulation circuits. Means 1 The filtration separation device of the present invention is a filtration separation device having a filter medium for separating substances into layers, and has a rotor near the filter medium on the raw solution side, and rotates to the filtrate side via the filter medium. It has a rotor operating body that operates a rotor, and at least when filtering an undiluted solution,
By operating the rotor with a rotor actuator, the clogging of the membrane is reduced, the risk of contamination of the stock solution side is reduced, and the above problems are solved.6 [Operation] Suspension containing high molecular weight substances as solutes. In order to perform filtration separation of the liquid, in the total filtration method in which filtration is performed perpendicular to the filtration surface, the filtration surface is rapidly clogged with suspended particles, so it is necessary to filter the filtration surface parallel to the S surface. The cross-flow filtration method, which performs filtration while forming a flow of Ti liquid, is effective.This method creates a flow along the filtration surface, which has the effect of reducing the accumulation of suspended particles. The higher the flow velocity against the surface, ie the greater the wall shear rate, the greater this effect.

Therefore, by rotating the rotor near the filtration surface, a 6-rotator, which can create turbulent flow against the filtration surface, increase the wall shear rate, and make it difficult for the filter to become clogged, is installed through the filter. Various methods can be considered for rotating the rotor operating body, but the rotor can be rotated by making the rotor and the rotor operating body with magnets and rotating the rotor operating body with a motor. preferable.

In this way, by rotating the rotor near the filtration surface, a turbulent flow is created on the filtration surface, increasing the wall shear rate and making it difficult for the filter to become clogged. As with the vertical filtration method, there is no need for a circulation circuit, so the risk of contamination of the inside of the reaction tank such as a fermenter or culture tank is very low6. It is desirable to appropriately set the wall shear rate for more effective filtration. The setting range of wall shear rate is 11.
It is preferable that it is 10-10000/see,
In other words, if the wall shear rate is less than 100, the effect of preventing particle adhesion cannot be obtained, and if the wall shear rate is more than 100%, if the suspension contains cell components, there is a possibility that this destruction may result, which is not preferable7. Furthermore, in order to make the above effects more effective, the average pore diameter of the filter medium must be 0.11115~+
5 ttm i8t It (H$ Porous membrane is preferred. The average pore size is less than n, 005 μm?
1.1 The overspeed decreases, making it impractical, and if the particle size exceeds 15 μm, the particle removal accuracy deteriorates.The pore diameter here is In other words, a liquid diluted to a solid content concentration of 0.1 wt% is passed through, and the capture efficiency is determined from the following formula.6 Capture efficiency (%): (Cf -Cp)/CfXl00Cf:
The latex concentration of the original liquid Cp: Find the trapping efficiency from the particles with the smaller particle size, and use the particle size when this value reaches 100% as the pore size6. Also, the porosity was calculated using the following formula.

Porosity (%) = pore volume Pa/porous body volume x 100 Materials for the porous tubular body include organic polymer materials such as fluorine-based and olefin-based materials, ceramics such as aluminum oxide and silicon nitride, Non-It like metals such as stainless steel
Any porous body may be used as long as it is made of a solid material or a mixture of both materials.

EXAMPLES Below, the present invention will be explained in more detail with reference to Examples, but the Examples are not intended to limit the present invention.

[Example] FIG. 1 shows a first example of the present invention. A ceramic membrane 12 and a membrane support 13 are attached to a part of the wall of the culture tank I+ so as to constitute a -ail chamber 14. Since the culture tank is sterilized by heat, it is preferable to use a ceramic membrane that is heat resistant.6Furthermore, a rotor 15 made of a magnet is installed on the opposite side of the filtrate chamber 14 via the membrane 12 to support the rotor. It is rotatably mounted by a body 16.

The structure of the rotating support 16 may be an angle structure, a net structure, a punched metal structure, or a combination of these structures, but a net structure is preferable, that is, the inside of the culture tank is always free. This effect can be reduced by covering the vicinity of the membrane 12 with a rotor support of a network structure, as this may inhibit the turbulent flow on the membrane surface caused by the rotor 15. It is from. A filtrate collection port 17 is attached to the filtrate chamber 14,
The filtrate collection port 17 is connected to a pump 18 . A rotor actuating body 19 made of a magnet is installed so as to face the rotor 15 via a ceramic membrane 12 and a filtrate chamber 14 . be rotated.

In addition, the culture tank 11. The membrane support 13 and the rotor support 16 are used to rotate the rotor 15 with the rotor operating body 19.
Preferably, it is made of non-magnetic material such as stainless steel, aluminum, or synthetic resin.

When measuring changes in the concentration of products or substrates in the culture tank 11, the rotor 15 is rotated by rotating the rotor operating body 19 to generate turbulent flow on the surface of the membrane 12 and increase the wall shear rate. At this time, the pump 18 is also operated at the same time, and the inside of the filtration chamber 14 is brought to 1 pressure, and the liquid in the culture tank 11 is filtered through the IIu 12 to collect a measurement sample. According to this method? Since the shear rate on the surface of the membrane 12 is always high at the time of 11 hours, clogging is unlikely to occur and continuous use for a long time is possible. A part of the rotor is made into a concave shape, and a cylindrical ceramic membrane 22 and a rotor support 26 are attached to the concave part so as to constitute a filtrate chamber 24. Furthermore, a magnetic rotor 25 is rotatably attached to the rotor support 26 outside the filtrate chamber 24 via the membrane 22 and the rotor support 26 . A filtrate collection port 27 is attached to the filtrate chamber 24 , and the filtrate collection port 27 is connected to a pump 28 . Rotor operating body 29 made of magnets
The cylindrical ceramic membrane 22 and the ML irf chamber 24
A rotor actuating body 29, which is disposed opposite to the rotor 25 via the rotor 25, is rotated by a motor 30. When measuring changes in concentration of products or substrates in the culture tank 21, a measurement sample is collected by filtering the liquid in the culture tank 21 through the membrane 22 in the same manner as in the first embodiment. In this case as well, as in the first embodiment, the wall shear rate can be increased on the surface of the membrane 22 due to the turbulent flow effect by the rotor 25, making it possible to use it continuously for a long time without causing clogging. becomes.

FIG. 3 shows a third embodiment of the invention. A ceramic membrane 32 and a membrane support 33 are attached to a part of the wall of the culture tank 31 so as to form a chamber 34.

Furthermore, a magnetic rotor 35 is rotatably attached to a rotor support 36 on the opposite side of the filtrate chamber 34 via the membrane 32 . A rotor operating body 39 made of a magnet is installed in the filtrate chamber 34 through the wall of the culture tank 31, and the rotor operating body 39 is rotated by a motor 40.

Since the wall of the culture tank 31 and the rotor operating body 39 are mechanically sealed, no filtrate leaks from the filtrate chamber 34. A filtrate collection port 37 is attached to the filtrate chamber 34 , and the filtrate collection port 37 is connected to a pump 38 . When measuring changes in the concentration of products or substrates in the culture tank 31, it is possible to increase the wall shear rate on the surface of the membrane 32 due to the turbulence effect of the rotor 35, as in the first embodiment. Therefore, a large amount of clogging occurs.<<,
Can be used continuously for a long time.

FIG. 4 shows a fourth embodiment of the invention. The cartridge of the filtration and separation device of the present invention is shown installed in the sample collection port 41 of the culture tank. The cartridge and body of the filtration separation device includes a membrane 42, a membrane support 43, a rotor 45, a rotor support 46, a filtrate collection port 47, a rotor operating body 49, and a motor 5.
It is composed of a 0.0 ring 51.

The membrane 42 is made of ceramic, and the membrane 42 and membrane support 43 constitute a filtrate chamber 44 . Furthermore, a rotor 45 made of a magnet is rotatably attached to the opposite side of the filtrate chamber 44 via the membrane 42 by a rotor support 46. The filtrate chamber 44 has a filtrate sampling port 47. Four rotor actuating bodies made of magnets, which are separated by one inch, are installed so as to face the rotor 45 with a ceramic membrane 42 and a liquid chamber 44 interposed therebetween. The rotor operating body 49 is rotated by the motor 50. The rotor operating body 49 is rotated by the motor 50.
The sample collection port 41 of the ridge and culture tank is connected to an O-ring 5I.
When measuring changes in the concentration of products or substrates in the 6 culture tanks that are liquid-tightly fitted together, the liquid in the culture tank is filtered through the membrane 42 in the same manner as in the first embodiment. In this case, as in the first embodiment, the wall shear rate can be increased on the surface of the membrane 42 due to the turbulent flow effect by the rotor 45, so that clogging of the membrane occurs. It is possible to use it continuously for a long time.

Furthermore, since the filtration separation device is a cart/soiler, it can be easily removed from the culture tank and the membrane 42 can be easily replaced. A filtration separation device having the structure shown in the first example was prepared using a two-open ceramic flat plate membrane, and the head was placed in a culture tank.

A filtration experiment using this filtration device was carried out using 6 LMi'a in the culture tank 11. Alcoholic yeast was cultured in the culture medium, and the bacterial weight was adjusted to 5% (wet weight). The sample was filtered through the ceramic flat membrane II by the pump 18, taken out as a measurement sample from the filtrate collection port 17, and the amount of filtration was measured.At this time, by rotating the rotor operating body 19 with the motor 20, When the rotor 15 was rotated at (Ollrpm) and the diaphragm indenter was adjusted to 0.04 g/cm', the initial filtration pupil was 3 m 17 minutes, and even 5 days after the start of filtration,
m1/min was maintained. In contrast, a filtration experiment was conducted under the same conditions as above without rotating the rotor 15, but
Clogging occurred rapidly, and 12 hours after the start of filtration, almost no liquid a could be obtained.

In addition, even if backwashing was performed, the initial flow rate was 115 or less two days after the start of filtration.9 [Effects of the Invention] As described above, the filtration separation device according to the present invention has the following advantages. have.

(By rotating the rotor near the filtering surface of the D filter medium, it is possible to create turbulent flow against the creeping surface of the D filter medium and increase the shear rate, which prevents clogging. Filtration or ultrafiltration can be done exclusively for a long time.

■It is safe because even if the circuit or pump used to collect the measurement sample is damaged, the inside of the reaction tank will not be contaminated.

[Brief explanation of drawings]

FIG. 1 is a cross-sectional view showing a first embodiment of the filtration and separation device of the present invention, FIG. 2 is a sectional view of the second embodiment of the filtration and separation device of the present invention, and FIG. 3 is a sectional view of the filtration and separation device of the present invention. FIG. 4 is a sectional view showing a fourth embodiment of the filtration and separation device of the present invention. ...Membrane 13, 33.43...Membrane support +4.24.34.44...J, 1 liquid chamber +5.25.
35.45...Rotor 1F, 26.36.46...Rotor support 17,
27.37.47...Filtrate collection port I11.28.38
...Pump 19, 29.39.49...Rotor operating body 20, 30
．． 40.5 (1... Motor 41... Culture tank sample collection port 51... ○ ring

Claims (11)

[Claims] (1) A filtration separation device having a filter material for separating substances, which has a rotor near the filter material on the raw solution side, and has a rotor operating body that operates the rotor on the filtrate side via the filter material. death,
A filtration separation device characterized in that clogging of a membrane is reduced by operating the rotor by the rotor operating body at least when filtering a stock solution. (2) The filtration and separation apparatus according to claim 1, wherein at least one of the rotor and the rotor operating body is composed of a magnet. (3) The filter medium for separating the substance has a pore size of 0.005 to 1
The filtration separation device according to claim 1 or 2, which is a microporous membrane of 5 μm. (4) The filtration separation device according to any one of claims 1 to 3, wherein the material of the filter medium that separates the substance is an inorganic material or an organic material. (5) installing the filter medium inside the tank wall of the reaction tank so as to form an independent space surrounded by the filter medium and the tank wall;
2. The filtration and separation apparatus according to claim 1, wherein the independent space is connected to a pump, and the components in the reaction tank are collected by the pump through the independent space through the filter medium. (6) The filtration separation device according to claim 5, wherein the rotor operating body is installed outside the tank wall. (7) The filtration separation device according to claim 5, wherein a part of the rotor operating body exists within the independent space. (8) It is composed of the filter medium, the filter medium support, the rotor, and the rotor operating body, and the filter medium and the filter medium support form an independent space on the filtrate side, and are detachably attached to the reaction tank. The filtration separation device according to claim 1, characterized in that: (9) The independent space has a filtrate collection port, the filtrate collection port is connected to a pump, and the components in the reaction tank are collected by the pump through the independent space via a filter medium. A filtration separation device according to claim 8. (10) The filtration separation device according to claim 8, wherein the rotor operating body is installed outside the independent space. (11) The filtration separation device according to claim 8, wherein a part of the rotor operating body exists within the independent space.