JPS6214267B2 - - Google Patents
Info
- Publication number
- JPS6214267B2 JPS6214267B2 JP60161352A JP16135285A JPS6214267B2 JP S6214267 B2 JPS6214267 B2 JP S6214267B2 JP 60161352 A JP60161352 A JP 60161352A JP 16135285 A JP16135285 A JP 16135285A JP S6214267 B2 JPS6214267 B2 JP S6214267B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- chamber
- cells
- tube
- electrodes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004027 cell Anatomy 0.000 claims description 40
- 230000004927 fusion Effects 0.000 claims description 28
- 230000007910 cell fusion Effects 0.000 claims description 21
- 125000006850 spacer group Chemical group 0.000 claims description 18
- 229910001220 stainless steel Inorganic materials 0.000 claims description 11
- 239000010935 stainless steel Substances 0.000 claims description 10
- 210000004754 hybrid cell Anatomy 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000002184 metal Substances 0.000 claims description 7
- 230000015556 catabolic process Effects 0.000 claims description 5
- 230000013011 mating Effects 0.000 claims 1
- 230000005684 electric field Effects 0.000 description 14
- 239000006285 cell suspension Substances 0.000 description 5
- 230000006835 compression Effects 0.000 description 5
- 238000007906 compression Methods 0.000 description 5
- 238000007499 fusion processing Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 238000005452 bending Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 229920002799 BoPET Polymers 0.000 description 1
- 239000005041 Mylar™ Substances 0.000 description 1
- 230000005679 Peltier effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- POIUWJQBRNEFGX-XAMSXPGMSA-N cathelicidin Chemical compound C([C@@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(C)C)C1=CC=CC=C1 POIUWJQBRNEFGX-XAMSXPGMSA-N 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 239000003818 cinder Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000004720 dielectrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000012804 iterative process Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000013021 overheating Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/635,009 US4578168A (en) | 1984-07-27 | 1984-07-27 | Apparatus for fusing live cells with electric fields |
| US635009 | 1984-07-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6137090A JPS6137090A (ja) | 1986-02-21 |
| JPS6214267B2 true JPS6214267B2 (https=) | 1987-04-01 |
Family
ID=24546052
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16135285A Granted JPS6137090A (ja) | 1984-07-27 | 1985-07-23 | 電気により生細胞を融合する装置 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US4578168A (https=) |
| JP (1) | JPS6137090A (https=) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3317415A1 (de) * | 1983-05-13 | 1984-11-15 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Kammer zur behandlung von zellen im elektrischen feld |
| DE3321238C2 (de) * | 1983-06-11 | 1985-05-02 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Verfahren und Vorrichtung zur Fusion von Zellen |
| US4622302A (en) * | 1984-08-09 | 1986-11-11 | American National Red Cross | Process for inducing membrane fusion under an electric field |
| DE3522610A1 (de) * | 1985-06-12 | 1986-12-18 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Kammer fuer die behandlung von zellen im elektrischen feld |
| KR890003947B1 (ko) * | 1985-12-11 | 1989-10-13 | 가부시기가이샤 시마즈세이사구쇼 | 세포융합장치 |
| JPH0673447B2 (ja) * | 1986-03-11 | 1994-09-21 | 株式会社島津製作所 | 細胞融合装置 |
| US4906576A (en) * | 1986-05-09 | 1990-03-06 | Electropore, Inc. | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor |
| US4923814A (en) * | 1986-05-09 | 1990-05-08 | Electropore, Inc. | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor |
| WO1987006851A1 (en) * | 1986-05-09 | 1987-11-19 | Electropore, Inc. | Apparatus and method for vesicle poration, loading and fusion |
| US4752284A (en) * | 1986-12-05 | 1988-06-21 | Biscar Jean P | Artificial gravity intracellular molecular extraction |
| JPS63169562A (ja) * | 1986-12-26 | 1988-07-13 | ダニエル・タイ−ユイ・ウオン | 高圧生物高分子転移および細胞融解装置 |
| US4970154A (en) * | 1987-10-09 | 1990-11-13 | Baylor College Of Medicine | Method for inserting foreign genes into cells using pulsed radiofrequency |
| US4822470A (en) * | 1987-10-09 | 1989-04-18 | Baylor College Of Medicine | Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses |
| EP0386086B1 (en) * | 1987-10-09 | 1996-05-29 | Baylor College Of Medicine | Method for cell poration and cell fusion using radiofrequency electrical pulses |
| US5389069A (en) * | 1988-01-21 | 1995-02-14 | Massachusetts Institute Of Technology | Method and apparatus for in vivo electroporation of remote cells and tissue |
| GB8807271D0 (en) * | 1988-03-26 | 1988-04-27 | Preece A W | Cell fusion apparatus |
| US5007995A (en) * | 1989-05-11 | 1991-04-16 | Olympus Optical Co., Ltd. | Device for electrofusion of cells |
| JPH0491700U (https=) * | 1990-12-27 | 1992-08-10 | ||
| US5173158A (en) * | 1991-07-22 | 1992-12-22 | Schmukler Robert E | Apparatus and methods for electroporation and electrofusion |
| JP2565463B2 (ja) * | 1993-02-19 | 1996-12-18 | 日本分光工業株式会社 | 電気的細胞操作装置のための操作チャンバー |
| IL108775A (en) * | 1994-02-25 | 2003-09-17 | Univ Ramot | Method for efficient incorporation of molecules into cells |
| AU5390696A (en) * | 1995-04-20 | 1996-11-07 | University Of South Florida | Purified and isolated sertoli cell aggregate |
| US5983131A (en) * | 1995-08-11 | 1999-11-09 | Massachusetts Institute Of Technology | Apparatus and method for electroporation of tissue |
| US5911223A (en) * | 1996-08-09 | 1999-06-15 | Massachusetts Institute Of Technology | Introduction of modifying agents into skin by electroporation |
| US6085115A (en) * | 1997-05-22 | 2000-07-04 | Massachusetts Institite Of Technology | Biopotential measurement including electroporation of tissue surface |
| IL133708A0 (en) | 1997-06-30 | 2001-04-30 | Rhone Poulence Rorer S A | Improved method for transferring nucleic acid into multicelled eukaryotic organism cells and combination thereof |
| AU8444798A (en) | 1997-06-30 | 1999-01-25 | Centre National De La Recherche Scientifique | Improved method for transferring nucleic acid into the striped muscle and combination therefor |
| USH2119H1 (en) | 1997-11-17 | 2005-06-07 | The United States Of America As Represented By The Secretary Of The Navy | Acoustic fusion of aquatic animal tissue cells with biological agents |
| FR2792207B1 (fr) * | 1999-04-15 | 2001-06-08 | Electricite De France | Procede de traitement d'un flux aqueux par electropulsation a champ parallele a l'ecoulement, chambre de pulsation et applications |
| KR100384924B1 (ko) * | 2000-10-12 | 2003-05-22 | 이석근 | 순환성 자기장을 이용한 유전자 발현 조절장치 및 그 방법 |
| DE10116211A1 (de) * | 2001-03-27 | 2002-10-10 | Eppendorf Ag | Kammer zur Behandlung von in einer Suspension enthaltenen Zellen im elektrischen Feld |
| DE10127247B4 (de) * | 2001-06-05 | 2006-12-07 | Eppendorf Ag | Vorrichtung und Verfahren zur elektrischen Behandlung suspendierter biologischer Partikel |
| IL151315A (en) * | 2002-08-18 | 2010-04-29 | Maroon J Abu Nassar | Fixture for electrode placement |
| EP3088516B1 (en) | 2003-12-01 | 2019-05-22 | Cellectis | Non-uniform electric field chamber for cell fusion |
| US8796021B2 (en) | 2007-02-23 | 2014-08-05 | Advanced Cell Technology, Inc. | Blastomere culture to produce mammalian embryonic stem cells |
| CN101912732B (zh) * | 2010-08-09 | 2012-02-01 | 天津富金环境技术研究有限公司 | 介电电泳法无污染回收贵重金属的高效连续工艺 |
| EP3194581A4 (en) | 2014-09-15 | 2018-04-25 | Children's Medical Center Corporation | Methods and compositions to increase somatic cell nuclear transfer (scnt) efficiency by removing histone h3-lysine trimethylation |
| CN108753569A (zh) * | 2018-05-25 | 2018-11-06 | 苏州博福生物医药科技有限公司 | 一种用于捕获细胞或生物分子的富集筛选机构 |
| CN113755318A (zh) * | 2021-09-03 | 2021-12-07 | 重庆大学 | 一种复合脉冲细胞电融仪和控制方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL128653C (https=) * | 1964-11-30 | |||
| GB1124185A (en) * | 1965-07-05 | 1968-08-21 | Mitsubishi Heavy Ind Ltd | A method of preventing adherence of marine life to a structure in sea water |
| US3984303A (en) * | 1975-07-02 | 1976-10-05 | Diamond Shamrock Corporation | Membrane electrolytic cell with concentric electrodes |
| US4269690A (en) * | 1980-01-28 | 1981-05-26 | Nancy Swartz Hammond | Electrolytic apparatus for reclaiming dissolved metal from liquid |
| US4441972A (en) * | 1983-04-08 | 1984-04-10 | D.E.P. Systems, Inc. | Apparatus for electrofusion of biological particles |
-
1984
- 1984-07-27 US US06/635,009 patent/US4578168A/en not_active Expired - Lifetime
-
1985
- 1985-07-23 JP JP16135285A patent/JPS6137090A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| US4578168A (en) | 1986-03-25 |
| JPS6137090A (ja) | 1986-02-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS6214267B2 (https=) | ||
| US4764473A (en) | Chamber for the treatment of cells in an electrical field | |
| US5173158A (en) | Apparatus and methods for electroporation and electrofusion | |
| US6150148A (en) | Electroporation apparatus for control of temperature during the process | |
| EP0225636B1 (en) | Apparatus for fusing cells | |
| US4441972A (en) | Apparatus for electrofusion of biological particles | |
| US4906576A (en) | High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor | |
| US4822470A (en) | Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses | |
| US20040115784A1 (en) | Apparatus and method for streaming electroporation | |
| KR101853514B1 (ko) | 부착 세포를 처리하기 위한 방법 및 전극 조립체 | |
| WO2000034434A1 (en) | Multi-channel electrode arrays | |
| US4699881A (en) | Chamber for the treatment of cells in an electric field | |
| DE3687905D1 (de) | Verfahren und einrichtung zur anregung einer fluessigkeit. | |
| CN111321077B (zh) | 用于大体积转染的装置和方法 | |
| US3205292A (en) | Heating and melting process of vitreous materials and furnace therefor | |
| US4959321A (en) | Cell fusion apparatus | |
| WO2008051169A1 (en) | Tip electrode chamber for small volume electroporation | |
| Techaumnat et al. | High-yield electrofusion of biological cells based on field tailoring by microfabricated structures | |
| CN210846406U (zh) | 活性炭再生装置和废气处理系统 | |
| KR20130037468A (ko) | 초소형 세포 융합장치 | |
| JPS63152972A (ja) | 電気的細胞操作のための高速電気細胞操作装置 | |
| JPH0239880A (ja) | 細胞処理チャンバ | |
| JP2013126381A (ja) | 細胞融合方法 | |
| WO1987006851A1 (en) | Apparatus and method for vesicle poration, loading and fusion | |
| JPS63152971A (ja) | 電気的細胞操作方法及びそのためのフロ−チヤンバ− |