JPS62134099A - ペルオキシダ−ゼ活性の測定方法 - Google Patents
ペルオキシダ−ゼ活性の測定方法Info
- Publication number
- JPS62134099A JPS62134099A JP60272426A JP27242685A JPS62134099A JP S62134099 A JPS62134099 A JP S62134099A JP 60272426 A JP60272426 A JP 60272426A JP 27242685 A JP27242685 A JP 27242685A JP S62134099 A JPS62134099 A JP S62134099A
- Authority
- JP
- Japan
- Prior art keywords
- value
- reagent
- reaction
- phenylenediamine
- opd
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000003992 Peroxidases Human genes 0.000 title claims abstract description 15
- 108040007629 peroxidase activity proteins Proteins 0.000 title claims abstract description 15
- 230000000694 effects Effects 0.000 title claims abstract description 12
- 238000005259 measurement Methods 0.000 title abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 35
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 8
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims abstract description 3
- 150000004986 phenylenediamines Chemical class 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 18
- 238000002835 absorbance Methods 0.000 abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 239000008213 purified water Substances 0.000 abstract description 5
- 239000007853 buffer solution Substances 0.000 abstract description 4
- 238000004040 coloring Methods 0.000 abstract 2
- -1 purified water Chemical compound 0.000 abstract 1
- 229910001868 water Inorganic materials 0.000 description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/20—Ortho-Phenylenediamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60272426A JPS62134099A (ja) | 1985-12-05 | 1985-12-05 | ペルオキシダ−ゼ活性の測定方法 |
| US06/936,320 US4855229A (en) | 1985-12-05 | 1986-12-01 | Measurement method of peroxidase activity |
| DE19863641489 DE3641489A1 (de) | 1985-12-05 | 1986-12-04 | Verfahren zur aktivitaetsbestimmung von peroxidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60272426A JPS62134099A (ja) | 1985-12-05 | 1985-12-05 | ペルオキシダ−ゼ活性の測定方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62134099A true JPS62134099A (ja) | 1987-06-17 |
Family
ID=17513744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60272426A Pending JPS62134099A (ja) | 1985-12-05 | 1985-12-05 | ペルオキシダ−ゼ活性の測定方法 |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US4855229A (https=) |
| JP (1) | JPS62134099A (https=) |
| DE (1) | DE3641489A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5215885A (en) * | 1990-10-23 | 1993-06-01 | Beckman Instruments, Inc. | Stable two-part chromogen substrate |
| US5182213A (en) * | 1991-03-18 | 1993-01-26 | Miles Inc. | Method for detecting the presence of peroxidatively active substance in basic media |
| CN110132913B (zh) * | 2019-04-24 | 2022-02-11 | 福建中医药大学 | 利用表面活性剂敏化反应进行铜离子检测的检测方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4193938A (en) * | 1978-03-08 | 1980-03-18 | Ppg Industries, Inc. | Extraction of phenylenediamine from aqueous alkaline solution |
| US4728608A (en) * | 1985-04-08 | 1988-03-01 | Cambridge Bioscience Corporation | Bacterial screening system with enhanced shelf life |
-
1985
- 1985-12-05 JP JP60272426A patent/JPS62134099A/ja active Pending
-
1986
- 1986-12-01 US US06/936,320 patent/US4855229A/en not_active Expired - Fee Related
- 1986-12-04 DE DE19863641489 patent/DE3641489A1/de active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE3641489C2 (https=) | 1991-01-31 |
| DE3641489A1 (de) | 1987-06-11 |
| US4855229A (en) | 1989-08-08 |
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