JPS6183127A - Production of solubilized material of thallophyte cell or bryophyte cell, prepared chemical cell solubilized material and its use as nutrition auxiliary - Google Patents

Abstract

PURPOSE:The titled cell liquefied material (CYTOLIZATE) useful as a nutrition auxiliary, etc., obtained by lending a cell of thallophte and/or bryophyte with a water-soluble salt of a specific metal at a specified temperature, heating, and cooling the salt. CONSTITUTION:A cell of a thallophyte (e.g., marine algae, mold (Aspergillus)) and/or a bryophyte (e.g., Hepaticae, Anthocerotae, or MUSUSHIMOSES) is blended with a water-soluble with a water-soluble salt of a metal of the first or the second main group of the periodic table in an amount enough to liquefy a cell bulk at 0-40 deg.C, then the liquefied mixture is heated and subjected to hemicytolish treatment at >=40 deg.C a sterilizing temperature, to give a liquefied material of thallophyte cell or bryophte cell. It is used as a feed, a fertilizer and/or a nutrition supplying agent for drinking water or sprinkling water, and amount of it used is 0.1-2wt% based on the feed, etc. The liquefied material is also used as a plant growing agent, cosmetic, enhancer for vitality, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION This invention relates to chemical liquefied products of cells of foliar plants and moss plants, and to a process for the production of such chemically liquefied cells, ie, hemitytrisate. These substances can activate the cellular metabolism of animal and plant organs, and can be used as additives in animal feed and food, and as growth agents for various plants. Products containing hemititolisate produced according to the present invention are highly efficacious and CACCC-factor (
CACCC is a drug that stimulates the proliferation of cells (abbreviation for chemically liquefied cells) based on chemically liquefied cells, which has the effect of activating cell metabolism.

Activators of cellular metabolism are known. For example, extracts from blood from which proteins and other higher molecular weight components have been removed,
Promotes cellular respiration in animal and human cells, and is located in isolated mitochondria (filament bodies) and increases their oxygen absorption (
Eger et al.
ng 19B5.750-4 pages).

Products made from calf blood extract with added heptaminol have been proposed to improve cellular respiration and brain function in humans. Gibberellins are known plant growth agents and can already influence the growth of many plants in very small amounts. However, it is extremely difficult to determine the amount to be used. Factors (extracts) that activate cellular substance metabolism
cannot be used as feed additives, as evidenced by experiments on them.

Its action as a feed additive, such as estrogen, antibiotics, arsenic and copper compounds, is significantly superior to the above extracts. Stimulants and regulators for feeding animals and plants, especially domestic animals and useful plants, are often used to improve their health and growth or to provide beneficial effects.

These are not in themselves nutrients or fertilizers for animals or plants, but are products that exert a preventive action and influence the metabolism of anabolic substances.

However, 111 effects have been observed in compounds and products previously developed in this regard, and their side effects are of great public concern today. This applies, for example, to compounds such as ticubacitracin (an antibiotic from the Veptlide group), the furan-based nitrobin (1,5-di(5-di(5-di(5-di)-2-furfurylamidine hydrazone, HCI), arsanilic acid (p- aminophenylarsinic acid), genonistrol diacetate (estrogen-based conductor), quinoxaline dioxid carbadox (methyl-3-(2-quinophthalinyl)-methylene)carbazade-N1,N4-dioxide), copper sulfate, etc. The same can be said about

Plant growth agents such as auxins, heteroauxins, gibberellins, etc., apart from the fact that their actions are extremely sensitive, some of them are extremely expensive, and are highly specific. It is extremely difficult to determine the amount to be paid.

The object of the present invention is to create a movable feed and nutrient additive that is based on natural materials (N-like plants, moss plants), does not have the side effects seen in the feed additives and plant growth agents mentioned above, and The purpose of this invention is to produce IJsate (cell liquefied product) for leafy plants and bryophytes, which is based on CACCC, which is a plant growth agent.

To this end, the present invention proposes a method for producing (i) IJsate from a foliar plant or a bryophyte as described in the claims.

Advantageous embodiments of this method are claimed in claims 3 to 1.
3. Hemititorisate obtained by these methods and their use as nutritional additives (
(expressed as a method of use) is defined in claim 14.
to 17 targets.

Surprisingly, cells of foliage plants and bryophytes treated by the hemitytolysis method (chemical liquefaction method)
It has been found to be an excellent basis for ACCC-products, which can be used as feed additives, nutritional additives, plant growth agents, and even as cosmetics and virility enhancers. And their uses extend to animals and plants.

Hemititorisate obtained by the process of the invention is non-toxic when tested by conventional toxicological methods and proved non-toxic by screening tests. LD5o is 20 g/kg or more by oral administration. No skin irritation was observed, especially in the rabbit eyes.

Its effectiveness as a virility enhancer is clear from the following observations and experiments. It is known that addition of 0.5% of ordinary untreated yeast to drinking water suppresses sexual desire in male mice. Such repressive properties can be achieved by the cytolysed yeast according to the present invention,
That is, when 0.15% of liquefied yeast (produced in Example 3) is added, it disappears. These results are summarized in Table 1. The products with hemititolizate of Examples 1 and 2 show similar results.

i Suppression of sexual desire in mice by yeast and compensation by cytolyzed enzymes - 50 male animals, 200 female animals (1:4
) The number of offspring per 50 births measured against a control without yeast is the suppression, young or exaltation rate. temperatures, especially 25"C to 35C
By mixing intimately with water-soluble salts of metals of the first and second main groups of the periodic table, especially mixtures thereof, and placing the material in sufficient quantity to liquefy the material, Process into chitorichish. Then the liquefied mixture.

Heat preferably rapidly to a temperature above 40' C but below the sterilization temperature of milk (82' C). After heating to the elevated temperature (heating continues for at least 10 minutes),
The mixture is cooled and, optionally mixed with a preservative, ready for use.

For example, the suspension of yeast thus obtained is adsorbed onto a solid silicic acid gel, if necessary, to form a form that is easy to handle.

In order to carry out the hemitytriche reaction, the materials described in detail below, as well as cells of plant microorganisms belonging to foliar plants and bryophytes, including bacteria, must be mixed with salts, especially those from the first and second groups of the periodic table. Treatment with salts of metals of the main groups or mixtures thereof. The water content then decreases.

As penetrating agents it is possible to use most salts whose water solubility is not too low. Sodium salts, potassium salts and Cal/Rum salts, and magnesium salts are preferred. In particular, salts in which the above-mentioned excellent metal ions and excellent anions are combined include sulfates, nitrates, chlorides, acetates, citrates, lactates,
These are maleate, succinate, bicarbonate and phosphate.

The quantitative proportion of the cellular material embedding mixture should use the lowest amount of salt mixture that is sufficient for liquefaction. The amount should be in the range 1.5-2.6% of the cellular material. 2°0%±0.2% is particularly good. In many cases, excess salt is not harmful.

It has been found useful to add additives such as carbohydrates and/or alcohols to the salt mixture. Suitable carbohydrates include glucose, saccharose, maltose, lactose, fructose, mannose, rubose, fucose, L-ascorbic. acids, glucosamine, gluconic acid, glucuronic acid, cyclodextrin, glycogen, soluble starch. Suitable alcohols are glycerin, glyfur, mannitol, sorbitol, ethanol, bentaerythritol. Salt mixtures and polyhydric alcohols. The combination of these is beneficial and excellent.

The first part of the IJ-se (chemical liquefaction) is carried out at a temperature of about 0°C to 40"C. After the action of the leaching agent, liquefaction of the cellular material is followed by a temperature of about 40"C. 70'C (but for cannabis it is best not to exceed 63°C)
Heating is performed to a certain temperature. The time required depends on the amount used, but it is best not to exceed 10 minutes.

Rapidly heat the liquefied product to the final temperature. This can be achieved by preheating the stirrer, for example to 70°C.

A similar method of operation is that the cell material is first heated under the conditions described above and then the salt mixture is added only after that.

It was found that when the proportion of metal salts in the second main group of the periodic table is about 109A compared to the metal salts in the first main group, the spider has an advantage.

If carbohydrates and/or alcohols are used in combination, the amount is in particular between 0.5 and 1.5 of that of the cell material before dehydration.
1 plate%.

The concept of liquefaction used in the present invention is known in the literature (Plaubelt, Vol. 12, 1946).
(pp. 183-185). In the context of the present invention, the term cytolyse refers to the dehydration of cells by the action of penetrants and heat. Krehun defined cytolysis as dehydration from cells due to heating, cold, or the action of osmotic agents, or fine contraction due to drainage. The premise of cytolyse is that the cell membrane has high extensibility, unlike the box-like rigidity of the cell wall of cells in the connective tissues of higher plants. A further important factor is the permeability to water, not to Plasmolytica, and the fact that the protoplasm becomes a gel with highly swellable colloidal properties upon expulsion of water.

What has been revealed by the present invention is that the following phenomenon occurs during the titrease according to the present invention.

1) Cytolyse in the classical sense (cresonone), but without significant plasmodesegregation.

2) Partial dehydration (hemitytlyse) from cells of suitable phyllophytes and bryophytes by leaching agents and heating.

3) Removal of inhibitors of growth, protein synthesis and sexual desire (hemititolyse) e 4) Formation of metabolic activators, once elevated existing activators, and formation of new activators (hemitito IJ-ase).

5) Protection of cell components by strengthening the cell membrane, ie, hemitytolyzed cells can have a debo effect. Alternatively, cells that are not hemitylysed can reach places that cannot be reached (cytolysed).

The above-mentioned effects of the hemitytolyses are particularly advantageous in the amounts used in the first or second main group of the periodic table (IJ).
It is particularly characteristic when using products made with salt mixtures containing salts of umum and magnesium. The addition of carbohydrates and/or alcohols, especially saccharoses and glycoses, enhances this hemitrichic effect.

According to the present invention, cells of foliar plants and bryophytes are treated to form hemichytotriches. Foliophytes include readily available and well-known seaweeds and molds, and bryophytes include readily available and well-known species such as Hebatica, Anthrocelotae, and Muscimoses.

Those belonging to the fungi include Phycomycetes, Algal and Quantum fungi (Ascomycetes, Sakufungi) (Satucharomyces, Aspergillus, Neurospora), Bastiomycetes, Crabfungi (Baxonia, Salurenoconcae, Polyphorus) I and 77 There are various classes of inverfecti.

There are the following classes of algae: Namely, green algae (chlorotheater, guri7 algani), yellow-green algae (chrysiphyta) (yellow-green
algae), golden-brown algae, pyrohophita, beophita (brown algae), cyanotwita (blue-green algae), rhodophyta (red algae), fission fungi (
Myxomycota) – Bacteria (Steredococcus, Bacillus, Spirillum) and Myxomycota
, and Bacillus subtilis.

Among the genus of yeast fungi, the following are particularly prominent in the class of quantum fungi.

Schizosaccharomyces (African Hirsebier, which also occurs in molasses), Endomyces lactis (milk mold, which appears in the base of cheese production), Saccharomyces (yeast in the narrow sense), and especially Saccharomyces cerevisiae (Buck yeast, Brennerei's yeast) and bottom-fermenting brewer's yeast), Satucharomyces cerevisiae pal ellipsoitheus (wine yeast), Satucharomyces carlsbergensis (bottom-fermenting brewer's yeast), Satucharomyces fragilis Noergenzen;
Hansenula yeast (e.g. Ecchi, Anorama Hansen-
7); Trichosporon cutaneum OTA (as a by-product of buck yeast and swallow yeast); Candida tropicalis Candida utilis Henneberg L. u. KR (Rulopuns utilis) (which is an industrial footer yeast and Neel yeast),
Kretsukera kursei (rum yeast), Berghout; Kretsukera Janke (wine must); Scholopolomycetes (getreid, juice and seawater, forage and straw);
Hansen).

Excellent hemitytrisates (those hemicytolysed according to the present invention) are yeasts in the narrow sense (Saccharomyces), industrial feed (footer) yeasts, and food (neel) yeasts (C. troplca11s, Torulopsls).
Obtained using ut1 mouth S).

Other excellent ascomycetes are Aspergillus niger and Neurospora clubsa.

Among seaweeds, Chlorella, Ninotia, Scenedesmus, Bacillus bifidus and Streptococcus are particularly good.

Among the moss, Platomoses, such as Minirum and Catalinaa, are outstanding.

The hemitytrisate produced according to the present invention can be obtained by treatment with a hemitytritic penetrating agent and heat treatment to obtain a
It is obtained as a suspension containing 5% solids. It can be diluted with water or aqueous solutions without any difficulty.

and about 0.0% in normal feed, drinking water, or both.
It is added in an amount of 1% to 2%. Such feed additives exhibit surprisingly good efficacy. For example, Aspergillus niger and Torula utilis (1:1) and salt mixture (
Gutsby fish reared on a diet containing hemitytrisate of the present invention based on a mixture of sodium and magnesium citrate, lactate and hydrochloride (Na:Mg, 10:1 weight ratio) After 4 weeks, the fish were already 85% larger than the normally reared control fish.

The effects of the CACCC-based products containing hemitytrisate of the present invention are both direct and indirect.

Indirect effects occur, for example, when a regulatory action occurs in warm-blooded and cold-blooded animals or in damaged microbial organisms, such as the iimm of leaf lice, i.e. the biological equilibrium is re-established; As a result, it occurs when nutrients are better utilized (without damaging the intestinal flora, leading to a state of equilibrium).

Imbalances in the intestinal flora of animals and humans can be caused by various reasons, such as luck! 11 (movement), abnormal weather, poor growth, changes in feed, and environmental changes are the causes. Due to such an amazing effect on enterobacteriaceae (drug effect), the hemititolisate-containing products produced according to the invention are also highly suitable for disease recovery.

The efficacy of the cell lines prepared according to the present invention for suitable targets is demonstrated, for example, in deletion rabbit experiments using the product prepared according to Example 3.

Using the growth parameters namely body weight, feed cost, water intake, and organ weight, 15 male and female rabbits (body 37 (1.4 kg to 2.0 kg Alpino rabbits, white) were tested. The growth status of New Norland rabbits was observed.

Two standard laboratory feeds and added bits of water (fasse)
r ad11bltu■). Test - The product was added to drinking water at a level of 0.1%. The rabbits were kept at 50-60% relative temperature at room temperature of 20°C.

Clinical results: The animals did not show any abnormalities compared to normal conditions after 6 weeks of treatment time (see Tables 2 and 3).

Body weight: Both males and females showed significantly good weight gain.

The increase was statistically significant (p less than 0.05 for males and less than 0.01 for females).
This is clear from Tables 4 and 5. The results in Tables 4 and 5 are shown graphically in the corresponding Figures 1 and 2. Feed consumption/drinking water consumption: There is no essential difference in overall consumption.

Anatomical findings: No differences from control animals (see Tables 6 and 7).

In the final dissection of the organ creation, the organs, heart, liver, both viscera and adrenal glands were dissected and weighed. For statistical evaluation,
Absolute organ weights were converted to relative organ weights in relation to body weight.

When comparing groups statistically, neither males nor females showed any statistical differences, nor did they show any group trends that were influenced by exposure.

It was possible to conclude that there was no burden due to the test product of the main portion. Tables 8 and 9 also prove this point.

Addition of 0.1% to drinking water significantly increased body weight gain and therefore sorption of feed utilization relative to control.

f-12- Appearance/! 1 degree Same reflex as normal observation
〃 Hair/Skin 1 Open parts of the body 〃 Funices (color luster, consistency)
〃Body weight〃Food/water intake〃Acute symptoms〃2-6-Organ Animal No., Findings Lungs
〃 〃heartki,/pericardial sac 〃 〃stomach
〃 〃Small intestine/large intestine
〃 〃liver 〃
〃Spleen 〃 〃
Kidney〃〃Serum/Vessel
〃〃Lymph node〃
〃Gonads〃
〃f deficiency---7= Organ Animal No., Findings ZNS
l-10 (5@5♀) No abnormality was observed macroscopically.Lungs〃〃Heart/Pericardium〃〃Stomach
〃 〃Small intestine/large intestine 〃 〃Liver〃
〃Spleen〃
〃Kidney 〃 〃Serum/Vessel 〃 〃Lymph nodes
〃 〃Gonads〃
The results of experiments on guinea pigs showed that the increase in feed efficiency was about 50%.

Feed efficiency for both groups: This corresponds to an improvement of 51% in the test experimental group.

No essential differences in feed intake and water intake were confirmed between the two groups.

The improvement in body weight compared to the control is evident from Tables 10-13.

Great hi! In one example, a superior hemitytrisate has been produced and tested as a feed additive or growth material.

Details of the drawings are explained in Example 4.

In step 5, Aspergillus niger and 5 kg of Torula utilis were mixed in a mixing aiit with an amount of salt mixture to liquefy the cell mass while stirring at 10°C. The salt mixture used was 10 parts by weight of citrate and/or lactate and/or hydrochloride of alkali metals (Na, K) and alkaline earth metals (Mg%Ca) to 0.4 parts by weight. It contains 20% saccharose/mannitol (1:1) based on its base weight.

Heat is applied to raise the temperature of the mixture to 56-59°C to complete hemitytlysis. Its to avoid infection! ! A suitable preservative such as nipagin (4-hydroxybenzoic acid methyl ester or 4-hydroxybenzoic acid ethyl ester) is mixed into the suspension. The resulting product contains approximately 35% dry matter.

The breeding test results for Gutsupi (grown between 15 and 30 years) are as follows:
2% of product per week. When added to regular diet, the fish were 95% larger than control fish fed regular diet after 4 weeks.

1 doctor - 1 kg of Neurospora crassosa, 1 kg of Lactobacillus bulgaricus, and 10 kg of Squid Geocolor yeast.

Sodium phosphate IJ at 30” C in a mixing apparatus with a heating device.
250 g of a mixture of Na 3 P O+, magnesium phosphate, sodium butyrate, glucose and manganese chloride in a weight ratio of 1:0.1:0.50:0.05:0.001 and 125 g of saccharose+ Mannyy) (1:1). Hemitytlysis started rapidly upon stirring and was terminated by heating to 80°C. After 1 hour, cool to 20°C and add 100g of silicic acid gel.
Mix with Aerosol 300 (finely ground, average diameter of Primer particles 7 gn) and Hinipagin/Hydroxyquinoline (preservative).

0.1% by weight of the product thus produced is added to Pulchini White Mountain chicken feed. Control and test animals are kept for 75 days and weighed on days 40 and 75.

Kotsuto Q-ni group 965 1.180
970 1,991 test group 990 1.258 989 2.19
After 0.075 days, the feed per kg of body weight was as follows: Control group: 2.73 kg Test group: 2.48 kg This corresponds to a feed saving of approximately 10%. The mortality rate in the test group is lower than in the control group. In other words, a beneficial effect was seen on the frog quality of chicken.

The protein and fat content of the test animal fish and tripe/
Digestion is essentially better than the control group.

Gontotoshi group 22.3 0.
79 1.98 test group 24.
2 G, 48 1.50 When the test animal fish was treated with trypsin for 90 hours, only 1.50 of the fish was treated with trypsin.
5% remained undigested (on the contrary, more than 1.98% remained undigested in the control group). and 25g sugar (saccharose) and heated to 60°C.

A suitable quenching agent was added.

After cooling, it was stored using Evagin/pentyl alcohol and Germal.

In order to convert the suspension into a solid, the suspension was adsorbed with a sufficient amount of finely ground silicic acid powder (see Example 2). A suitable quantitative ratio is 21 yeast suspension per 300 g of 1-3 g aerosol.

Approximately 0.2-0.5% of the solid product so obtained was added to the rat diet. As a result, feed intake is reduced and
Therefore, the feed utilization rate improved.

Growth parameters are double duty, food consumption, water loss intake, and organ weight as shown below. The feed is standard laboratory feed Althromin.

The rearing conditions were a room temperature of 22°C, an air relative humidity of 50-80%, and a daily irradiation time of 12 hours.

Body weight and food consumption are summarized in Tables 14-16.

Feed Efficiency (FE): If the FE number increases, it indicates an increase in body weight or a decrease in food consumption.

The feed consumption of the test group was correspondingly significantly lower than the weight gain of the controls, so that an improved utilization of the feed could be recognized.

The feed efficiency is as follows.

Control group 0.21 Test group 0.24 Each expressed as weight gain per Ig feed intake over 6 weeks.

2moni L ball Y -1 H 02 control 1
818 test group l
EXAMPLE 1 Hemicytrisate prepared according to the present invention according to the procedure of 725 Engineering Co., Ltd. was used.

Wine yeast, baker's yeast, and bottom-fermenting beer yeast (8
100 parts by weight of a mixture of potassium magne/rum and sodium chloride and saccharose and gluconic acid in a ratio of 0.3:0.2:1.7:0.
Mix with 2.5% by weight of a mixture containing 2=0.1 while stirring.

Starting temperature 22℃ 0 Final temperature 52-6 by rapid heating
Up to 2℃. At 40"C 2 parts by weight of silicic acid gel powder (see Example 2) and 5 parts by weight of mannitol are added.

After foaming is finished and cooled to 20° C., the product is ready for use when adding it to the rearing water.

If you have to store this product for a long time, please use 0.1
It is advisable to add a preservative in an amount of ~0.3%. This product can be stored for at least one year. ! ! ! Since it is cloudy, shake it before use.

The following test method was chosen to evaluate hemititrisate as a plant growth agent.

Part of fine sand and part of peat were used as plant soil. Unless otherwise specified in the table below, the plants were planted on May 20th. The product hemitytrisate was added to the irrigation water at a ratio of 1/1000.

Test cultivation was performed on the defective plants, and co-/troult comparison observations were made. On July 1st, ie, after about 6 weeks of purification with mold solution, evaluation was carried out by taking photographs. However, the only thing I planted on June 16th was the Japanese cypress, and the photo was taken on July 8th.
I did a ffl shadow. As a result, it was revealed that the growth of each of the following 17 aM products was significantly promoted by the hemicytrisate of the present invention.

(1) Chi7ya (2) Chair (3) Tomato (8! Seedling size 20cm) (4) Targetes (1115-20c) (5) Bechuni (11)
20c Seal) (6) Ezogiku (// 3c++) (7) Ageratum (// 20c■) Both left and right sides are products of the present invention, center = control (8) Begonia (Size of seedlings 1)
5cm) (9) Begonia (〃) (10) Ento (Kashiwa 4.2615.5) (11)
) Umorokon (〃) (12) Sunflower (〃) (13) Sunflower (seed stick 4.25) (14) Ento (〃) (15) Bechuni (size of seed market 15c mouth) (16)
Senjugiku (May seed stick) (17) Sennoyugiku (〃)

[Brief explanation of drawings]

1 and 2 are graphs showing the effects of the present invention. Attached photos 1-17 all show the plant growth effect of the present invention (there is no other way to prove this effect other than by photos) Photo 1c1: Chinya photo 2: Chair photo 3 Nidomado (size of seedlings) 20cm) Photo IL4:
Targetes (II 15-15-2O Photo 5: Bechuni (// 2Qc嘗) Photo 6: Ezogiku (〃3c)
Photo 7: Agera Thiem (20c irises) Both left and right are products of the present invention, center = control Photo 8: Begonia
(Size of 81 seedlings 15cm) Photo 9: Begonia (〃
) Photo 10: Peas (ffl sown 4.2515.5) Photo 11: Corn (〃) Photo 12: Sunflowers (〃) Photo 13: Sunflowers (Ffl sown 4.25) Photo 14: Peas (〃) Photo 15: Petuni (seedling size 15cm) Photo 1
6: Senji Yugiku (May broadcast) Photo 17 Nisenji Yugiku (〃) Agent Patent Attorney (8334) Sunagawa Okabe (1 person)” Kobi7 Yamamasa; 1υ. July 31, 1985

Claims (1)

[Scope of Claims] (1) Cells of foliar plants and/or bryophytes are treated at a temperature range of 0°C to 40°C in an amount sufficient to liquefy cell aggregates in the first and second main cells of the periodic table. A foliar plant produced by mixing with a water-soluble salt of a group metal or a mixture of those salts, then heating the liquefied mixture to a temperature above 40°C but below the sterilization temperature, and then cooling. Liquefied cell material of bryophyte. (2) Sufficient water solubility of metals from the first and second main groups of the periodic table to liquefy the cell aggregates of foliar plants and/or bryophyte cells at a temperature range of 0°C to 40°C. A foliar plant, characterized in that it is mixed with a salt or a mixture of these salts, then treated into a hemitrichtrich by heating the liquefied mixture to a temperature above 40°C but below the sterilization temperature, and then cooled. Method for producing a liquefied product of cells or bryophyte cells (3) The method according to claim 2, characterized in that the liquefied mixture is heated to a temperature in the range of 40°C to 70°C. (4) A method according to claim 2 or 3, characterized in that the liquefied mixture is heated to a temperature of up to 63°C. (5) A method according to claims 2 to 4, characterized in that the liquefied mixture is heated uniformly and rapidly at a rate of at least 10° C./min. (6) The method according to claims 2 to 5, characterized in that the liquefied mixture is heated for at least 10 minutes. (7) The method according to claims 2 to 6, characterized in that carbohydrates and/or alcohols are additionally added to the liquefied mixture. (8) The method according to claims 2 to 7, characterized in that a salt mixture containing sodium salts and calcium salts and/or magnesium salts is used. (9) characterized in that sulfates, nitrates, chlorides, acetates, citrates, lactates, maleates, succinates, carbonates, bicarbonates and/or phosphates are used as salts; The method according to claim 8. (10) The method according to claims 2 to 9, characterized in that about 1.5 to 2.5 parts by weight of salt is mixed with 100 parts by weight of the cell aggregate. (11) 0 per 100 parts by weight of cell aggregates in the liquefied mixture
．． 11. Process according to claims 2 to 10, characterized in that 5 to 1.5 parts by weight of glucose and/or saccharose are added. (12) The method according to claims 2 to 11, characterized in that the cell material is selected from the group consisting of Aspergillus niger, Torrautilis, Neorospora crassa, Lactobacillus bulgaricus, Saccharomyces, and Icasiocara yeast. (13) Saccharomyces cerevisiae with 2% Na by weight
12. Process according to claims 2 to 11, characterized in that liquefaction is carried out with Cl, 0.3% by weight of MgSO_4 and 0.5% by weight of saccharose. (14) Use of cell liquefaction product (hemitytrisate) of foliar plants or bryophytes in an amount of 0.1 to 0.5% as feed, fertilizer and/or nutritional supplement for drinking water or irrigation. (15) Use of hemititrisate as a plant growth agent. (16) Use of hemicytrisate as a cosmetic to activate skin metabolism. (17) Use of hemitytrisate as a drug for sexual enhancement.