DE3402169A1 - Process for the preparation of thallophytic or bryophytic cytorrhysates, chemicytorrhysates prepared thereby, and the use thereof for nutrient supplementation - Google Patents

Abstract

Published without abstract.

Description

Process for the production of thallophytic

or bryophytic cytorrhysates, chemicytorrhysates prepared thereafter and their use for nutritional supplementation. The invention relates to manufacture of chemytorrhysed cells of thallophytes and bryophytes and the like Chemicytorrhysates, which affect the cell metabolism of animal and plant organisms can activate, in the sense of food and feed additives for humans and animals, in the sense of plant growth substances for all kinds of plants Art. Preparations which contain the chemicytorrhysates produced according to the invention, are exceptionally potent cell proliferation stimulants on the Basis of CACCC factors (CACCC stands for "cell metabolism activating chemicytorrhysized cells ").

Factors that activate cell metabolism are known. For example promotes an extract freed from proteins and other high molecular weight components cellular respiration from blood animal and human cells and increases oxygen uptake in isolated mitochondria (Jaeger et al., Arzneimittelforschung 1965, pp. 750-4). To improve cell respiration and brain function in humans are Preparations from calf blood extracts with added Hept-UnoL have been proposed. Gibberellins are well-known plant growth substances that stimulate the growth of many plants can have a strong influence even in very small amounts; however, their dosage is difficult. The ones that activate cell metabolism are used as feed additives Factors (extracts) are out of the question, as corresponding tests have shown.

The effects of estrogens, antibiotics, arsenic and copper compounds as a feed additive is clearly superior to the extracts mentioned.

Stimulators or regulators for feeding animals and plants, especially domestic animals and crops, are more and more in use around to improve health and growth and to positively influence them. There are Preparations that are not themselves food or nutrients for animals or plants which have a prophylactic effect and the anabolic metabolism influence.

However, for all compounds developed so far in this context and preparations side effects have been observed, to which now intensified attention by the public facing. This applies, for example, to compounds such as zinc bacitracin (an antibiotic belonging to the peptolide group ), for nitrovin (1,5-di (5-nitro-2-furfuryl) -1 , 4-pentadien-3-one-amidinhydrazon.HCl) of the furan group, for arsanilic acid (p-aminophenylarsinic acid), for dienoestrol diacetate (an estrogen derivative), for carbadox (methyl-3- (2-quinoxalinyl) -methylene) carbazate-N¹, N4-dioxide) from the quinoxaline dioxide group, for copper sulfate, etc.

The pronounced plant growth substances such as auxins, heteroauxins, Gibberellins and others are very concentration-dependent and difficult in their effect to dose, quite apart from the sometimes high price and their specificity.

The invention is based on the object, thallophytic and bryophytic Manufacture of cytorrhysates based on CACCC, exclusively from natural materials (Thallophyta, Bryophyta) are free of side effects that the previous, Above feed additives and plant growth substances adhere to, and effective Represent food and feed additives and plant growth substances.

To this end, the invention proposes the method specified in claim 1 for the production of cytorrhysates of thallophytes and bryophytes. Preferred Refinements of this method are the subject of subclaims 2 to 12. The chemicytorrhysate obtainable by this process and its use for the nutrient gnuns are the subject of claims 13 to 17.

Surprisingly, it has been shown that treated by chemicytorrhysis Thallophytes and bryophytes an excellent one Basis for CACCC preparations that are used as feed additives, food additives, plant growth substances, and even useful as cosmetics and aphrodisiacs. Your application extends on the animal and plant sector.

The chemicytorrhysates obtained by the process according to the invention proved to be non-toxic according to the usual toxicological methods and in the screening test as non-toxic.

The LD50 for oral administration is over 20 () / kg. Egg irritations, in particular in rabbit eyes, could not be observed.

The usability as an aphrodisiac results from the following observations and experiments: it is known that yeast inhibits the sexuality of male mice, when normal untreated yeast is added to drinking water in an amount of 0.5% This inhibition is lifted when the drinking water 0.15 t of the invention cytorrhysed ilefe (prepared as in Example 3. The results are summarized in Tab. Preparations with the chemicytorrhysates of the examples 1 and 2 showed similar results.

Table I: Inhibition of sexuality in mice by yeast and compensation by cytorrhysed yeast - 50 male animals and 200 female animals per experiment. Animals (1: 4) after cancellation d.

Trial number d. Inhibition of inhibition d. Addition of a series of male sexuality d. Corrupted yeast * No. mice normal yeast (8) * inhibition (%) increase (2 1 50 (1: 4) 70 0 20 2 50 (1: 4) 65 10 3 50 (1: 4) 60 0 25 * inhibition or increase measured on the number of juniors per 50 litters, based on controls without yeast in the case of the invention Procedures are intact thallophyte cells, bryophyte cells, or both chemicytorrhytic treated by keeping the cells at temperatures in the range of about o0c to 40 ° C, preferably at 250C to 35 ° C, intimately with a water-soluble salt and preferably a salt mixture are mixed from salts of the metals of the 1st and 2nd main group of the periodic table, wherein the amount of salt is such that it liquefies the amount of cell bodies sufficient.

Thereafter, the liquefied mixture, preferably quickly, on a Heated temperature that is above 40 ° C, but below the pasteurization temperature of milk should be (82 "C). After heating to an elevated temperature, this is preferred If the mixture lasts at least 10 minutes, it is cooled down, possibly with preservatives offset, and is ready to use. It is advantageous to use the finished yeast suspension to be taken up in solid silica gel and thereby in an inexpensive, sandable form bring to.

The cells are used to initiate the chemicytorrhytic reactions of the materials mentioned in detail below and others plant organisms belonging to the thallophytes and bryophytes, -including of the bacteria, with salts, preferably with salt mixtures of salts of metals 1st and 2nd main group of the periodic table treated.

This reduces their water content.

Most salts can be considered as osmocitica, some are not have too little water solubility, the sodium and potassium salts are preferred as well as the calcium and magnesium salts. Preferred anions, especially in combination with the aforementioned preferred metal ions, are sulfates, nitrates, chlorides, acetates, Citrates, lactates, malates, succinates, carbonates, bicarbonates and phosphates.

The proportions of cell material to salt mixture are general adjusted so that a minimum amount of salt mixture that is sufficient for liquefaction, is used.

This amount is preferably in the range from 1.5 to 2.5% of the cell body material; 2.0% + 0.2% are especially preferred. In most cases, an excess is harmful but not of salt.

It has been found useful to add an additive to the salt mixtures to add carbohydrates and / or alcohols.

Suitable carbohydrates are glucose, sucrose, maltose, lactose, Fructose, mannose, sorbose, fucose, L-ascorbic acid, glucosamine, gluconic acid, glucuronic acid, Cyclodextrins, glycogen, soluble starch. Suitable alcohols are: glycerine, glycol, Mannitol, sorbitol, ethanol, pentaerythritol. A combination of salt mixture and polyvalent ones Alcohols are cheap and preferred.

The first part of chemicytorrhysis occurs at temperatures of about OOC to 400C carried out. After exposure to the Osmocitica, following the Liquefaction of the cell material heated to temperatures, which are preferably between 400C and 700C, but mostly and preferably not exceeding 630C. The length of time depends in detail on the amount used, it should however, preferably not less than 10 minutes.

Preferably, the liquefied preparation quickly reaches the final temperature heated, which is advantageously achieved by preheating the stirred vessel, e.g. to 700C can be.

Another, albeit less preferred, way of working is to to first heat the cell material (under the conditions specified above) and only then add the salt mixture.

It's been found to be particularly cheap when the stake of the salt of a metal of the 2nd main group about 10% of the amount of the salt of the metal or the metals of the 1st

Main group.

With the use of carbohydrates and / or alcohols amounts to their amount to preferably 0.5 to 1.5% by weight of the cell body mass (before dehydration).

The term cytorrhysis used here is known from the literature (See magazine Die Brauwelt ", Issue 12, 1946, pp. 183-185). In connection with In the present invention, the term cytorrhysis stands for dehydration from cells by osmotica and exposure to heat. Kressin defines cytorrhysis as the contraction of cells by dehydration or water release from cells under the influence of heat, cold or Osrnotica. The prerequisite for cytorrhysis is a cell membrane that is in contrast to the almost box-like strength of the cell wall of a tissue group Cell of a higher plant has a high elasticity. Furthermore are important Factors the permeability for water, but not for plasmolytics and the fact that the protoplasm by releasing water to form a gel with the properties of a highly swellable colloid.

In the course of the invention it was found that when the invention Cytorrhysis carry out the following processes: 1) cytorrhysis in the classic sense (cressin), without significant plasmolysis.

2) Partial dehydration from the cells of suitable Thallophyta and Bryophyta by osmotica and heat (chemicytorrhysis).

3) Elimination of inhibitors (inhibitors) of growth, protein synthesis and sexuality (chemicytorrhysis).

4) Formation of metabolic activators, both increasing the concentrations existing as well as formation of new activators (chemicytorrhysis).

5) Protection of cell components through membrane reinforcement so that the Chemizytorrhysierten cells can develop a depot effect or get to places that cannot be reached by the non-cytorrhysed cells (cytorrhysis).

These effects of chemicytorrhysis are particularly pronounced in Preparations made with salt mixtures in the preferred amounts are which salt mixtures both salts of the 1st main group and the 2nd main group

Main group, preferably containing salts of sodium and magnesium. The addition of carbohydrates and / or alcohols, preferably sucrose and glucose, intensifies this chemicytorrhytic effect.

According to the invention, cells of thallophytes and bryophytes become chemicytorrhytic treated. Thallophytes include the well-known and freely accessible algae and fungi, Bryophytes the known and freely accessible classes of the Hepaticae (liverworts), Anthrocerotae (horne liverworts) and Musci mosses.

The fungi include the classes of Phycomycetes algal fungi (Mucor, Pythium, Phytophora) and Ascomycetes sac fungi (Saccharomyces, Aspergillus, Neurospora) and the Basidiomycetes club fungi (Puccinia, Amanita, Polyphorus) and Fungi imperfecti.

The algae include the following classes: Chlorophyta -green algae, Chrysophyta - yellow-green algae, golden brown algae, Pyrrhophyta, Phaeophyta - brown algae, Cyanophyta - blue-green algae, Rhodophyta - red algae, Schizomycota - bacteria (Streptococcus, Bacillus, Spirillum) and Myxomocyta - slime mollds.

From the class of the Ascomycetes come from the genus of the yeast-like Fungi preferred the following species in question: Schizosaccharomyces (millet beer in Africa, also occurring in molasses); Endomyces lactis (milk mold that is considered a beneficial insect occurs during cheese ripening); Saccharomyces (yeast in the strict sense), namely Saccharomyces cerevisiae (baker's yeast, distillery yeast and bottom-fermented brewer's yeast), Saccharomyces cerevisiae var.ellipsoideus (wine yeast), S. carlsbergensis (bottom-fermented Brewer's yeast), S. fragilis JORGENSEN; S. lactis DOMBROWSKI, S. rosei GUILIERMONT, S. biosoprus NAGARISHI; Hansenula yeasts such as H.anomala HANSEN-SYDOW Trichosporon cutaneum OTA (as a companion to baking and fodder yeast) -; Candida tropicalis and C. utilis HENNEBERG L. and KR (Torulopsis utilis), which are technical feed and nutritional yeasts; Candida Krusei (cream yeast) BERKHOUT; Kloeckera JANKE (wine musts); Sporobolomycetes (Grain, fresh and sea water, hay and straw); and Pichia polymorpha LOCKER (Pichia HANSEN).

Preferred chemicytorrhysates are with the yeasts in the strict sense (Saccharomyces) and technical feed and nutritional yeasts (C. tropicalis, Torulopsis utilis).

Further preferred Ascomycetes are Aspergillus niger and Neurospora crassa.

The species Chlorella, Nitschia, Scenedesmus, Bacillus bifidus and Streptococcus are preferred.

Among the mosses, leaf mosses (e.g. Mnium and Catharinea) are preferred.

The chemicytorrhysates produced according to the invention are used as suspensions with approx. 30 to 35% solids content according to the chemizytorrhytic osmotica and Received heat treatment. You can easily use water or aqueous solutions be diluted. They are preferred as nutrient supplements or growth substances in Amounts of about 0.1 wt. To 2% are added to normal feed or drinking water or both.

Such feed additives sometimes show amazing results. Such were guppies that were reared on a food that contained a chemicytorrhysate the invention based on Aspergillus niger and Torula utilis (1: 1) and 2 up to 4 wt. t of a salt mixture (mixture of citrates, lactates and chlorides of the Sodium and magnesium with a Na: Mg weight ratio of 10: 1) already contained after 4 weeks 95% larger than normally fed control fish.

The effect of the chemicytorrhysates according to the invention containing CACCC-based preparations can be direct and / or indirect. An indirect effect occurs, for example, if a regulating influence on harmful microorganisms, E.g. the intestinal flora of warm and cold-blooded animals, or of aphids occurs, see above that the biological balance can be restored and thus a better one Utilizing the nutrients can be done (balancing the intestinal flora without them to destroy).

Such imbalances in the intestinal flora of animals and humans can be caused by the most varied of circumstances, e.g. by transport, adverse Seasons, insufficient growth, changes in food and changes in the environment.

Preparations containing chemytorrhysates produced according to the invention are suitable due to this surprising effect on the intestinal flora (drug bers Effect) to a particular extent also for convalescence.

The effectiveness of the cells cytorrhysed according to the invention is more suitable Objects are shown, for example, in the rabbit experiment described below, in which the preparation prepared according to Example 3 has been used.

Using the growth parameters: body weight, feed consumption, Water intake and organ weight were the growth behavior of 15 rabbits each male and female

Sex (albino rabbits, white New Zealanders) with weights of 1.4 kg to 2.0 kg observed. The standard laboratory diet: Höing and water ad libitum. The test preparation was added to the drinking water in an amount of 0.1%. the Rabbits were at room temperature of 200C and rel. Humidity of 50 - 60% held.

Clinical picture: The animals showed after the 6-week treatment period no deviations from normal behavior (evident from the following Tah. II and III).

Body weights: males and females showed significantly improved Weight gain that is statistically significant was (p less than 0.05 for males, less than 0.01 for females), as can be seen from Tables IV and V. is. The results of Tables IV and V are shown in the corresponding Fig.

1 and 2 shown graphically.

Feed consumption / water consumption: No significant differences in Total consumption.

Autopsy findings: No deviations from the control animals (Tab. VI and VII).

Organ weights: At the final secretion the organs heart, liver, both kidneys and adrenal glands dissected and weighed.

The absolute organ weights were used for the statistical evaluation transformed into relative organ weights in relation to the body weights.

The statistical comparison of the group means showed neither in the Males still have statistical differences or dose-dependent group trends among females.

A load on the main excretory organs from the test preparation could thus be excluded, as is also shown in Tables VIII and IX.

An addition of 0.1% to the drinking water caused a significantly increased Weight gain and thus improved feed utilization compared to the controls.

Table II Weekly Report for Clinical Observations Test Week: 1st - 6 group: control animal no .: 1- 10 sex: 5 # 5 # examination macroscopically compilation of findings Appearance / behavior no deviations from normal observations, reflections, no deviations from normal observations hair coat / skin turgor no deviations from normal observations Body orifices no deviations from normal observations Faeces (color, consistency) no deviations from normal observations body weights food / water consumption no deviations from normal observations Acute symptoms no deviations from Normal observations Table II Weekly Report for Clinical Observations Test week: 1 - 6 Group: II Animal No .: 1- 10 Sex: 5 # 5 # Examination macroscopically Compilation of findings appearance / behavior no deviations from normal observations Reflexes no deviations from normal observations Hair dress / skin turgor no deviations from normal observations body orifices no deviations from normal observations Faeces (color, consistency) no deviations from normal observations body weights Food / water consumption no deviations from normal observations Acute symptoms no deviations from normal observations Table IV Summary: Body weights (g) # Group Initial 1st week 2nd week 3rd week 4th week 5th week 6th week Weight gain (total) K # 1635 1829 2081 2247 2451 2642 2813 1174.9 s # 291 258 205 247 271 235 241 213.2 II *) # 1712 1978 2278 2519 2762 2762 2933 30771365.0 **) s # 145 183 218 212 234 255 199 106.6 *) Test preparation from example 3 **) P <0.05 Table V Summary: Body Weights (g) # Group initial 1st week 2nd week 3rd week 4th week 5th week 6th week increase weight (total) K # 1709 1943 2194 2365 2533 2727 2868 1160.1 s # 272 243 259 267 267 267 250 62.0 II # 1655 1946 2274 2541 2809 3038 3213 1556.9 s # 124 100 125 134 148 135 139 58.3 * P <0.01 II = test preparation from example 3 Tabel VI end section (macroscopic findings) group = control organ animal no. Findings CNS 1 - 10 (5 # 5 #) macroscopically without trial sp. Changes in the lungs "macroscopically without trial sp. Changes in the heart / pericardium "macroscopically without trial sp. Changes in the stomach "macroscopically without any experimental changes in the small / large intestine "macroscopically without experimental changes liver" macroscopically without experimental changes. Changes in the spleen "macroscopically without experimental changes in the kidneys" macroscopically without trial sp. Changes in seroses / vessels "macroscopically without any experimental changes Lymph nodes "macroscopically without any experimental changes in the gonads" macroscopically without trial sp. Changes Table VII End Section (macroscopic Findings) group = control organ animal no. Findings CNS 1 - 10 (5 # 5 #) macroscopically without trial sp. Changes in the lungs "macroscopically without any experimental changes Heart / pericardium "macroscopically without experimental changes in stomach" macroscopically without trial sp. Changes in the small and large intestine "macroscopically without any experimental changes Liver "macroscopically without experimental changes. Spleen" macroscopically without experimental changes. Changes in kidneys "macroscopically without any experimental changes in seroses / vessels "macroscopically without experimental changes in lymph nodes" macroscopically without trial sp. Changes in the gonads "macroscopically without any experimental changes Tabel VIII Relative organ weights (%) # Heart Liver Kidney left. Kidney right. Adrenal gland left Adrenal gland right

K control 1 0.23 3.92 0.28 0.32 0.006 0.005 2 0.22 3.10 0.32 0.32 0.004 0.005 3 0.25 3.97 0.28 0.28 0.006 0.004 4 0.22 3.47 0.29 0.30 0.004 0.006 5 0.23 3.50 0.31 0.29 0.004 0.005 # 0.23 # 3.59 # 0.30 # 0.30 # 0.005 # 0.005 s 0.01 s 0.36 s 0.02 s 0.02 s 0.001 s 0.001 II *) 1 0.22 3.95 0.29 0.32 0.004 0.006 2 0.22 3.62 0.31 0.30 0.005 0.004 3 0.22 3.09 0.32 0.30 0.004 0.003 4 0.24 3.41 0.30 0.32 0.004 0.004 5 0.25 3.98 0.28 0.29 0.004 0.003 # 0.23 # 3.61 # 0.30 # 0.30 # 0.0042 # 0.0040 s 0.01 s 0.38 s 0.02 s 0.01 s 0.0004 s 0.0010 *) II = test preparation Tabel IX Relative organ weights (%) # Heart Liver Kidney left Kidney right Adrenal gland left Adrenal gland re.

K control 1 0.25 3.72 0.30 0.30 0.004 0.005 2 0.26 3.24 0.31 0.32 0.005 0.005 3 0.20 3.84 0.33 0.32 0.005 0.004 4 0.19 3.21 0.28 0.30 0.004 0.004 5 0.23 3.03 0.33 0.30 0.005 0.004 # 0.22 3.41 0.31 0.31 0.0050 0.0050 s 0.03 0.35 0.02 0.01 0.0010 0.0010 II- *) 1 0.22 3.06 0.29 0.26 0.004 0.004 2 0.22 3.84 0.26 0.28 0.006 0.003 3 0.24 3.67 0.29 0.28 0.004 0.003 4 0.20 3.17 0.31 0.30 0.005 0.005 5 0.24 3.36 0.31 0.28 0.005 0.004 # 0.22 3.53 0.29 0.28 0.0050 0.0040 s 0.02 0.26 0.02 0.01 0.0010 0.0010 *) II = test preparation A corresponding one Trial with guinea pigs resulted in an increase in feed effectiveness of approx. 50 t.

Feed utilization of the groups: male. Sea female Guinea pig pig control 0.063 0.080 test group 0.093 0.123 This corresponds to an improvement the group "test attempt" by 51%.

Regarding food and water consumption, a difference was made between the two Groups found no significant differences.

The improvement in body weight compared to the controls results from Tab. X to XIII.

Table X Body Weights (g / week) Group: Control - ## Animal No. Initial weights 1st week 2nd week 3rd Woxhe 4th week 5th week Increase 1 786 790 799 840 870 910 126 2 470 490 540 550 570 590 120 3 460 475 500 510 530 570 110 4 510 540 550 590 635 655 144 5 614 630 640 675 705 735 120 6 674 700 714 745 780 805 130 7 450 505 535 560 585 620 170 8 540 545 590 615 630 655 115 9 490 525 535 570 605 630 140 10 506 525 550 585 615 660 155 # 550.0 572.5 595.5 624.0 652.6 683.0 133.0 SD # 109.2 102.2 95.3 101.2 103.9 105.1 19.18 Table XI Body weights (g / week) Test group: - ## Animal No. Initial weight 1st week 2nd week 3rd week 4th week 5th week Increase 1 490 544 585 605 670 695 206 2 455 505 540 575 645 670 215 3 470 490 535 570 610 645 175 4 560 585 635 670 710 745 185 5 480 520 560 595 635 675 195 6 640 670 695 735 760 795 155 7 610 635 674 710 740 785 175 8 470 495 540 565 585 635 165 9 675 685 730 770 795 830 155 10 520 550 570 606 670 680 160 # 537.0 568.0 606.5 640.0 682.0 715.5 178.5 *) SD # 79.6 72.6 71.9 75.2 67.9 68.2 21.09 *) <0.01 Table XII Body Weights (g / week) Group: Control - ## Animal No. Initial Weight 1st Week 2nd Week 3rd Week 4th Week 5th Week Increase 1 570 625 630 650 695 735 165 2 550 620 660 660 700 720 170 3 495 520 535 555 580 605 110 4 710 755 760 770 805 840 130 5 400 420 465 505 530 560 160 6 380 395 435 475 520 545 165 7 410 435 470 505 510 550 140 8 670 675 690 730 750 785 115 9 560 605 615 645 655 690 130 10 460 505 550 585 635 665 205 # 520.5 55.5 581.0 608.0 638.0 669.5 149.0 SD # 125.5 119.2 107.4 99.6 101.7 103.1 29.23 Tabel XIII body weights (g / week) test group: - ## animal no. Initial weight 1st week 2nd week 3rd week 4th week 5th week Increase 1 580 656 669 736 806 829 251 2 520 590 640 675 715 765 246 3 570 630 660 690 730 770 199 4 655 705 735 760 780 805 150 5 470 525 570 630 680 715 245 6 370 431 490 550 600 645 275 7 430 470 496 545 585 635 206 8 480 535 575 640 685 730 250 9 460 495 550 585 645 680 220 10 540 565 590 630 655 685 146 # 507.5 560.0 597.5 644.0 688.0 726.0 218.5 *) SD # 82.6 86.2 78.5 72.5 71.5 66.1 43.7 *) @ 0.01 In the following examples Preferred chemicytorrhysates are produced and used as food additives or Tested growth substances. Details of the figures are explained in Example 4.

Example 1 5 kg of Aspergillus niger and 5 kg of Torula utilis are used in 100C with stirring in a suitable mixing device with such an amount of one Salt mixture added that the mass liquefies. The salt mixture contains Citrates and / or lactates and / or chlorides of the alkali metals (Na, K) and alkaline earth metals (Mg, Ca) in a ratio of organic matter to salt of 10 parts by weight to 0.4 parts by weight, and an additional 20% sucrose / mannitol (1: 1), based on the amount of salt.

With the addition of heat, the temperature of the mixture increases to 56 - 59 "C increased to complete chemytorrhysis. To avoid contamination the suspension is treated with suitable preservatives, e.g. with nipagin (4-hydroxybenzoic acid methyl ester or 4-hydroxybenzoic acid ethyl ester). The preparation obtained contained approx.

35% dry matter.

The growth test on guppy fish that were 1 week old showed if 2 o / oo of the preparation per week can be added to the normal feed that the After 4 weeks, animals were 95% larger than normally fed control fish.

Example 2 1 kg of Neurospora crassa, 1 kg of Lactobacillus bulgarocis and 10 kg of Japanese Ikashiokara yeast are mixed at 30 ° C. in a mixer which has a means of heating with 250 g of a mixture of sodium phosphate (Na3PO4), magnesium phosphate.

Sodium lactate, glucose and manganese chloride (weight ratio 1: 0.1: 0.05: 0.05: 0.001) and 125 g sucrose + mannitol (1: 1) added. Chemicytorrhysis occurs rapidly with stirring one, which is finished by heating to a temperature of 600C. After 1 hour cooled to 200C with 100 g silica gel Aerosil 300 {finely ground, dust, avg. Diameter of the primary particles 7nm) and with nipagin / hydroxyquinoline (for preservation) offset.

The preparation produced in this way is added to the feed of Pulcini White mountain Chicken added at 0.1% (weight basis). Control animals and Test animals are fed for 75 days and weighed on day 40 and day 75: after 40 days after 75 days animal weight animal weight group number (kg) number (kg) control group 965 1,180 970 1,991 Trial group 990 1,258 989 2,190 The feed consumption per kg Body weight after 75 days is calculated as follows: Control group 2.73 kg test group 2.48 kg corresponding to a feed saving of approx. 10%. The mortalities in the test group are lower than in the control group, i.e. the health of the chicken is positively influenced.

Also the protein and fat content and the trypsin digestion of the meat the test animals are much better than those of the control group:% protein% Fat% digestibility ~ ~ by trypsin control group 22.3 0.79 1.98 experimental group 24.2 0.48 1.50 Only 1.5% of the meat remains after 90 hours of trypsin treatment of the meat of the test animals undigested (compared to 1.98% for the control animals) Example 3 5 kg of Saccharomyces cerevisiae are mixed with a mixture of 100.degree. C. at 350.degree g NaCl, 15 g MgSO4 and 25 g cane sugar (sucrose) are added and heated up to 60 ° C.

A suitable antifoam agent is added.

After cooling, nipagin is used to preserve benzyl alcohol and Germall.

In order to bring the suspension into a solid form, it is mixed with the sufficient amount of finely divided silica gel dust (see. Example 2) added. A The appropriate proportion is 2 ml of yeast suspension per 1-3 g of Aerosil 300.

Approx. 0.2 to 0.5% of the solid preparation obtained in this way was added to the feed of rats added. The result was a reduced feed intake and thus an improved one Feed utilization.

The growth parameters were: body weights, feed consumption, water intake, Organ weights. Food: standard laboratory dietitian Altromin.

Conditions: room temperature 220C, rel. Humidity 50 - 60%, daily lighting duration 12 hours.

Body weights and feed consumption are summarized in Tables XIV to XVI.

Feed effectiveness: The FE number is an indication of either if it is increased to increased weight gain or decreased feed consumption.

Because the feed consumption in the test group was significantly lower when the weight gain corresponded to the control, it can be improved from here Feed utilization can be assumed.

The feed efficiencies were as follows: Control: 0.21 Test group: 0.24 in each case expressed as the weight gain in 6 weeks per lg of feed intake.

Table XIV Summary: Body Weights (g) Group Initial 1. Week 2nd Week 3rd Week 4th Week 5th Week 6th Week Weight gain (total control 87.9 129.2 168.8 199.2 227.2 249.0 261.8 173.9 # 3.7 # 5.6 # 7.3 # 9.0 # 13.2 # 18.1 # 22.1 # 21.1 Test group 86.6 124.8 165.8 198.2 222.8 244.3 262.5 175.9, # 3.6 # 4.9 # 5.5 # 6.3 # 8.4 # 9.8 # 10.2 # 11.9 Table XV Summary: Feed consumption (g / week) Group 1st week 2nd week 3rd week 4th week 5th week 6th Week Total Control 112.5 128.7 147.5 135.0 141.2 152.0 816.8 # 2.5 # 3.3 # 4.1 # 8.3 # 10.5 # 12.0 # 34.2 Test group 103.3 115.3 132.8 126.2 134.8 146.8 770.9 * # 3.6 # 7.6 # 3.1 # 7.4 # 9.9 # 12.8 # 43.0 * p <0.05 Tabel XVI Individual feed consumption over 6 weeks Group animal no. Total (g) control 1 818 2 797 3 772 4 836 5 872 6 810 Test group 1 725 2 723 3 793 4 816 5 816 6 752 example 4 In this example, a chemicytorrhysate prepared according to the invention was used, obtained as follows: 100 parts by weight of a mixture, the wine yeast, baker's yeast and bottom-fermented brewer's yeast in a ratio of 8: 90: 2, is stirred with 2.5 parts by weight of a mixture that contains the chlorides of potassium and magnesium and sodium as well as sucrose and gluconic acid in the ratio of 0.3: 0.2: 1.7: 0.2: 0.1 contains.

Initial temperature: 220C. Final temperature after rapid heating up to 52-62 ° C. At 400C, 2 parts by weight of finely ground silica gel (see example 2) and 5 parts by weight of mannitol were added. After the end of the foaming and after Cooling down to 200C, the preparation can be used immediately if it is added to the irrigation water adds.

If the preparation has to be stored for a longer period of time, it is advisable to add a preservative in an amount of 0.1 to 0.3%. The preparation can then be kept for at least 1 year. Always shake around before use as it is is a suspension.

The following was used to evaluate the chemicytorrhysate as a plant growth substance Experimental arrangement chosen: 1 part fine sand and 1 part peat were used as the plant soil used. Unless otherwise noted, it was planted on May 20th. The preparation (Chemizytorrhysat) was added to the Gießw.1sser in a quantity of 1 per mille.

The attached photos show a comparison with one with the chemicytorrhysate the plant treated according to the invention (left in each case) in comparison with a control plant (Each on the right). The following are shown in detail: Photo 1: Lettuce (seedling on May 20th) Photo 2: Zinnia (seedling on May 20th) Photo 3: Tomatoes (seedling on May 20th cm tall) Photo 4: Tagetes (seedlings on May 20th, 15-20 cm tall) Photo 5: Petunias (seedlings on May 20 20 cm tall) Photo 6: Summer aster (seedling on May 20, 3 cm tall) Photo 7: Ageratum (Seedlings on May 20, 5 cm tall) left and right preparation according to the invention, middle = Control Photo 8: Begonia (seedlings on May 20th, 15 cm tall) Photo 9: Begonia (seedlings on May 20 15 cm tall) Photo 10: Peas (planted from seeds on April 25th / 5th) Photo 11: Maize (planted from seeds on April 25/5) Photo 12: Sunflowers (planted from seeds) on April 25th 5.

Photo 13: Sunflowers (planted from seeds on April 25th) Photo 14: Peas (planted from seeds on April 25th) Photo 15: Petunias (seedlings on April 25th, 15 cm tall) Photo 16: Tagetes erecta (seedlings planted in May) Photo 17: Tagetes erecta (seedlings planted in May) The images are photographs taken on July 1st, i.e.

after approx. 6 weeks watering with the preparation solution, provided the seedlings on May 20 were planted. Photo 16 was taken on June 16. and LoLo 17th on 8th July recorded.

It can be seen that the chemicytorrhysate produced according to the invention to an essential: 'leads to greater growth of the respective plants.

- L e r s e i t e -

Claims (17)

Claims 1. A method for producing a thallophytic or bryophytic cytorrhysate, characterized in that intact thallophytic and / or bryophyte cells are chemizytorrhytisch treated by the cells at Temperatures in the range from 00r to 400C intimately with a water-soluble salt or Salt mixture of metals of the 1st and 2nd Main group of the periodic table in one for liquefying the cell body mass be mixed in a sufficient amount and then add the liquefied mixture a temperature is heated which is above 40 ° C, but below the pasteurization temperature and then cooled. 2. The method according to claim 1, d a d u r c h g e k e n n z e i c h n e t that the liquefied meat is at a temperature in the range from 400C to 700C is heated. 3. The method according to claim 1 or 2, characterized in that on a temperature of 630C or less is heated. 4. The method according to any one of claims 1 to 3, characterized in that that the liquefied mixture is heated uniformly and quickly, preferably with a Speed of at least 100 / min. 5. The method according to any one of claims 1 to 4, characterized in that that the mixture is heated for at least 10 minutes. 6. The method according to any one of claims 1 to 5, characterized in that that the mixture additionally carbohydrates and / or alcohols are added. 7. The method according to any one of claims 1 to 6, characterized in that that a salt mixture is used, the sodium salt (s) and calcium and / or magnesium salt (s) contains. 8. The method according to claim 7, characterized in that the salts as sulfate, nitrate, chloride, acetate, citrate, lactate, malate, succinate, carbonate, Bicarbonate and / or phosphate can be used. 9. The method according to any one of claims 1 to 8, characterized in that that with about 1.5 to 2.5 parts by weight of salt based on 100 parts by weight of intact Cell body mass, is mixed. 10. The method according to any one of claims 1 to 9, characterized in, that the mixture glucose and / or sucrose in an amount of 0.5 to 1.5 parts by weight can be added per 100 parts by weight of intact cell body mass. 11. The method according to any one of claims 1 to 10, characterized in, that the cell material from the group: Aspergillus niger, Torula utilis, Neurospora crassa, Lactobacillus bulgaricus, Saccharomyces species and Japanese Ikashiokara yeast is selected. 12. The method according to any one of claims 1 to 10, characterized in that that Sac Aromyces cerevisiae with 2 wt. t NaCl, 0.3 wt. t MgSO4 and 0.5 wt .-% sucrose is liquefied. 13. Chemicytorrhysate obtained by a method of the claims 1 to 12. 14. Use of the chemizytorrhysate according to claim 13 for nutritional supplementation for feed and / or drinking water in quantities of 0.1 to 0.5 t. 15. Use of the chemizytorrhysate according to claim 13 as a plant growth substance. 16. Use of the chemizytorrhysate according to claim 13 as a cosmetic active ingredient to activate the skin metabolism. 17. Use of the chemizytorrhysate according to claim 13 as an aphrodisiac to increase potency.