JPS6182105A - Setting device for initial position of fine stylus or the like - Google Patents

Abstract

PURPOSE:To position a fine stylus extremely accurately by setting the zero-cross point of the S-shaped output waveform of a differential photodetector based upon the movement of a fine body, etc., at an initial position where it should be set previously. CONSTITUTION:The differential photodetector provided to the stage 6 of a microscope is provided with a pair of a light emitting element 11, such as an LED, and a differential photodetecting element 12, such as a differential solar battery, which are provided at x-axial distance from each other and a pair of a light emitting element 13 and a differential photodetecting element 14 which are provided at z-axially distance. When an extremely small glass stylus 13 is moved in the z-axial direction, S-shaped output wavelength shown in a graph is obtained by the pairs 11 and 12 and the z coordinate z0 of the zero-cross point is known; when the glass stylus 3 is moved in the x-axial direction, the x coordinate x0 of the zero-cross point is known. For the purpose, the differential photodetector 1 is installed on the stage 6 so that the zero- cross point (x0, z0) has (x) and (z) coordinates corresponding to one point in an area within the visual field of the microscope, and then the glass stylus 3 is only moved so as to detect this zero-cross point during operation, thereby obtaining the (x) and (z) coordinates of a glass bar 3.

Description

DETAILED DESCRIPTION OF THE INVENTION (A) Field of Industrial Application The present invention relates to an initial position setting device for microneedles, etc., and is used for setting microneedles to initial positions in, for example, micromanipulation. It is something.

(B) Prior Art When a microscopic object such as a cell is manipulated using a micromanipulator, for example, a manipulation instrument such as a microscopic glass needle must first be inserted into the field of a microscope in a container holding the sample. However, the tip of this microneedle has a diameter of several μm.
It is extremely thin and will break if you touch it with a prepared slide.

Conventionally, setting the initial position of the microneedles has been done by visual measurement, which is difficult unless someone is skilled in operating a micromanipulator.

(c) Purpose of the Invention The present invention solves the drawbacks of the above-mentioned prior art, and enables the initialization of microneedles, etc., which can automatically and reliably set the microneedle ring at the initial position within the field of view of a microscope in micromanipulation, etc. The purpose is to provide a position setting device.

(d) Structure of the Invention The present invention provides a differential photodetector including a light emitting element such as an LED, a differential photodetection element, etc., and a microscopic body such as a microneedle so as to perpendicularly cross the light beam from the light emitting element. and a means for reading the output of the differential photodetector as the microscopic object such as the microneedle moves, and detects the zero cross of the S-shaped output waveform of the differential photodetector due to the movement of the microscopic object. This is an initial position setting device for microneedles, etc., characterized by setting a point at an initial position to be set in advance.

(E) Embodiment FIG. 1 is a configuration diagram showing an embodiment of the present invention, FIG. 2 is a plan view of a differential photodetector, FIG. 3 is a front view of the differential photodetector, and FIG.
The figure is a diagram showing an example of an output waveform from a differential photodetector.

A differential photodetector 1 is installed on a microscope stage 6, and a micromanipulator 2, which is a means for moving a microscopic object such as a microscopic glass needle 3, detects the microscopic glass needle 3 in the y-axis direction in the figure. By inserting the microscopic glass needle 3 into the device 1 and moving the microscopic glass needle 3 in the X-axis direction and the X-axis direction shown in the figure with respect to the differential photodetector 1, the position of the X coordinate and 2 coordinates that fall within the field of view of the microscope is detected. However, if the micro glass needle 3 is further linearly moved in the y-axis direction at the determined position of the X coordinate and 2 coordinates, the micro glass needle 3 can be moved straightly in the y-axis direction without contacting the operation container 4 etc.
can be brought into the field of view of the microscope. 5 is an objective lens of the microscope.

To further explain the configuration of the device, a differential photodetector 1 installed on the stage 6 of the microscope has a light emitting element 11 such as an LED spaced apart in the X-axis direction and a differential photodetector such as a differential solar cell. A pair of photodetecting elements 12 and a pair of a light emitting element 13 and a differential photodetecting element 14 provided spaced apart in two axial directions are provided. Therefore, if the micro glass needle 3 is moved in two axial directions, pair 11
．． 12, an S-shaped output waveform as shown in FIG. 4 can be obtained, and two coordinates zO of the zero cross point can be found. In addition, if the micro glass needle 3 is moved in the X-axis direction, the pair 1
3.14, it is also possible to obtain an S-shaped output waveform as shown in FIG. 4 and find the X coordinate XQ of the zero crossing point.

Therefore, conversely, if the differential photodetector 1 is installed on the stage 6 in advance so that this zero crossing point (xo, zo) is at two coordinates with the X coordinate corresponding to one point in the field of view of the microscope, By simply moving the glass micro needle 3 to detect this zero-crossing point during operation, the X and 2 coordinates of the micro glass needle 3 that can be inserted into the field of view of the microscope can be obtained. The y-coordinate within the field of view of the microscope can be set to a desired position y by moving the microscopic glass needle 3 in the y-axis direction while viewing the microscope. That is, the initial position of the micro glass needle 3 is (xo, yt.

20) can be obtained. In order to automatically set the initial position, as shown in FIG. A device is configured by combining a discriminator 8 that determines whether the device has moved.

(F) Effect The present invention has the above-described configuration, and can easily and quickly move a microscopic body such as a microneedle to an initial position to be set. Furthermore, since a differential photodetector consisting of a light emitting element and a differential photodetection element is used, the position of a microneedle etc. can be set very accurately. Therefore, by incorporating this device into a coarse movement mechanism control system of a micromanipulator, it becomes very easy to set the initial position of a microneedle, etc.

[Brief explanation of the drawing]

FIG. 1 is a configuration diagram showing an embodiment of the present invention, FIG. 2 is a plan view of a differential photodetector, FIG. 3 is a front view of the differential photodetector, and FIG.
The figure is a diagram showing an example of an output waveform by a differential photodetector. 1--Differential photodetector 2--Micromanipulator 3--Micro glass needle 4--Operation container 5--Objective lens 6--Stage 7--Output reader 8--Discriminator 11.13 -・Light emitting element

Claims (1)

[Claims] A differential photodetector consisting of a light emitting element such as an LED and a differential light detection element, a means for moving a microscopic body such as a microneedle so as to perpendicularly cross the light beam from the light emitting element, and a means for moving a microscopic body such as a microneedle, etc. means for reading the output of the differential photodetector as the microscopic object moves, and sets the zero-crossing point of the S-shaped output waveform of the differential photodetector due to the movement of the microscopic object to a preset initial position. An initial position setting device for a microneedle, etc.