JPS6161069B2 - - Google Patents
Info
- Publication number
- JPS6161069B2 JPS6161069B2 JP8742678A JP8742678A JPS6161069B2 JP S6161069 B2 JPS6161069 B2 JP S6161069B2 JP 8742678 A JP8742678 A JP 8742678A JP 8742678 A JP8742678 A JP 8742678A JP S6161069 B2 JPS6161069 B2 JP S6161069B2
- Authority
- JP
- Japan
- Prior art keywords
- trypsin
- agglutination
- bacterial cells
- treated
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000004520 agglutination Effects 0.000 claims description 36
- 230000001580 bacterial effect Effects 0.000 claims description 35
- 238000006243 chemical reaction Methods 0.000 claims description 28
- 102000004142 Trypsin Human genes 0.000 claims description 24
- 108090000631 Trypsin Proteins 0.000 claims description 24
- 239000012588 trypsin Substances 0.000 claims description 24
- 239000000427 antigen Substances 0.000 claims description 20
- 102000036639 antigens Human genes 0.000 claims description 20
- 108091007433 antigens Proteins 0.000 claims description 20
- 230000002269 spontaneous effect Effects 0.000 claims description 8
- 229940122618 Trypsin inhibitor Drugs 0.000 claims description 6
- 101710162629 Trypsin inhibitor Proteins 0.000 claims description 6
- 239000002753 trypsin inhibitor Substances 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 description 18
- 241000287828 Gallus gallus Species 0.000 description 12
- 235000013330 chicken meat Nutrition 0.000 description 12
- 241000606767 Avibacterium paragallinarum Species 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000607142 Salmonella Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241000271566 Aves Species 0.000 description 3
- 241000589567 Brucella abortus Species 0.000 description 3
- 241000589874 Campylobacter fetus Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000204022 Mycoplasma gallisepticum Species 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 229940056450 brucella abortus Drugs 0.000 description 3
- 239000007975 buffered saline Substances 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 230000002458 infectious effect Effects 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000016784 immunoglobulin production Effects 0.000 description 2
- 206010039083 rhinitis Diseases 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 206010033971 Paratyphoid fever Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000607729 Salmonella enterica subsp. enterica serovar Abortus Species 0.000 description 1
- 241000607662 Salmonella enterica subsp. enterica serovar Abortusequi Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000006161 blood agar Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 229940039227 diagnostic agent Drugs 0.000 description 1
- 239000000032 diagnostic agent Substances 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8742678A JPS5513884A (en) | 1978-07-17 | 1978-07-17 | Aggregation reacting antigen and its manufacture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8742678A JPS5513884A (en) | 1978-07-17 | 1978-07-17 | Aggregation reacting antigen and its manufacture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5513884A JPS5513884A (en) | 1980-01-31 |
| JPS6161069B2 true JPS6161069B2 (enrdf_load_stackoverflow) | 1986-12-24 |
Family
ID=13914538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8742678A Granted JPS5513884A (en) | 1978-07-17 | 1978-07-17 | Aggregation reacting antigen and its manufacture |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5513884A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63149279U (enrdf_load_stackoverflow) * | 1987-03-20 | 1988-09-30 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5980981A (ja) * | 1982-11-01 | 1984-05-10 | Sanyo Electric Co Ltd | ガリウム燐緑色発光ダイオードの製造方法 |
-
1978
- 1978-07-17 JP JP8742678A patent/JPS5513884A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63149279U (enrdf_load_stackoverflow) * | 1987-03-20 | 1988-09-30 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5513884A (en) | 1980-01-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lancefield | Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test | |
| Stoenner et al. | Antigenic variation of Borrelia hermsii. | |
| Lancefield | A serological differentiation of human and other groups of hemolytic streptococci | |
| Amies | The envelope substance of Pasteurella pestis | |
| Kunin et al. | Serological studies of O antigens of Escherichia coli by means of the hemagglutination test | |
| Pauli et al. | Studies on the serological typing of Streptococcus hemolyticus | |
| JP2595133B2 (ja) | 免疫刺激剤およびその製造法 | |
| Hughes | The epidemiology of fowl cholera: II. Biological properties of P. avicida | |
| Kantor et al. | Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin | |
| Beck | The role of the spirochaete in the Wassermann reaction | |
| Seastone | The occurrence of mucoid polysaccharide in hemolytic streptococci of human origin | |
| Blair et al. | Streptococcal agglutinins and antistreptolysins in rheumatoid (atrophic) arthritis | |
| Sottile et al. | Application of counterimmunoelectrophoresis in the identification of Streptococcus pneumoniae in clinical isolates | |
| JPS6161069B2 (enrdf_load_stackoverflow) | ||
| Young et al. | Sensitization of infant red cells by bacterial polysaccharides of Escherichia coli during enteritis | |
| Edmunds et al. | Two cases of listerial meningitis in infants | |
| Miller et al. | Haemophilus somnus Complex: Antigenicity and Specificity of 1 Fractions of Haemophilus somnus | |
| Wallace et al. | The bentonite flocculation test in the assay of Neisseria antibody | |
| Rake et al. | STUDIES ON MENINGOCOCCUS INFECTION: IV. The Antigenic Complex of the Meningococcus—Group-Specific Carbohydrate and Protein Fractions | |
| Barnes et al. | Serological relationship between pneumococcus Type I and an encapsulated strain of Escherichia coli | |
| Nakazawa et al. | Diagnosis of Rhodococcus equi infection in foals by the agar gel diffusion test with protein antigen | |
| JPH0664065B2 (ja) | 菌体外膜保有菌の免疫学的測定法 | |
| RU2754465C1 (ru) | Способ получения эритроцитарного диагностикума для реакции непрямой гемагглютинации (рнга) при клостридиозах животных | |
| Kolmer | A study of diphtheria bacilli with special reference to complement-fixation reactions | |
| SU889003A1 (ru) | Способ получени диагностической псевдотуберкулезной сыворотки |