JPS6151737B2 - - Google Patents
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- Publication number
- JPS6151737B2 JPS6151737B2 JP53106850A JP10685078A JPS6151737B2 JP S6151737 B2 JPS6151737 B2 JP S6151737B2 JP 53106850 A JP53106850 A JP 53106850A JP 10685078 A JP10685078 A JP 10685078A JP S6151737 B2 JPS6151737 B2 JP S6151737B2
- Authority
- JP
- Japan
- Prior art keywords
- layer
- chemical analysis
- reagent
- multilayer chemical
- liquid sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000004458 analytical method Methods 0.000 claims description 126
- 239000000463 material Substances 0.000 claims description 112
- 239000000126 substance Substances 0.000 claims description 106
- 239000003153 chemical reaction reagent Substances 0.000 claims description 79
- 239000007788 liquid Substances 0.000 claims description 51
- 238000003892 spreading Methods 0.000 claims description 28
- 230000007480 spreading Effects 0.000 claims description 28
- 238000011161 development Methods 0.000 claims description 20
- 238000009825 accumulation Methods 0.000 claims description 18
- 239000000523 sample Substances 0.000 description 52
- 238000000034 method Methods 0.000 description 25
- 238000000151 deposition Methods 0.000 description 13
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 230000008021 deposition Effects 0.000 description 8
- 239000000306 component Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000004445 quantitative analysis Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000004040 coloring Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- -1 polyethylene terephthalate Polymers 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004078 waterproofing Methods 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 229930185605 Bisphenol Natural products 0.000 description 1
- 229920008347 Cellulose acetate propionate Polymers 0.000 description 1
- 229920001747 Cellulose diacetate Polymers 0.000 description 1
- 229920002284 Cellulose triacetate Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 229920001328 Polyvinylidene chloride Polymers 0.000 description 1
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 1
- 239000012503 blood component Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920006217 cellulose acetate butyrate Polymers 0.000 description 1
- 238000009614 chemical analysis method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000005137 deposition process Methods 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 201000003373 familial cold autoinflammatory syndrome 3 Diseases 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000005033 polyvinylidene chloride Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002940 repellent Effects 0.000 description 1
- 239000005871 repellent Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229920002050 silicone resin Polymers 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Description
【発明の詳細な説明】
本発明はシート状の多層化学分析材料集積板お
よびその使用方法に関するもので、さらに詳しく
は、複数の異種の多層化学分析材料が支持板面上
に少なくともその試薬層が互いに接触又は近接し
て配置された構造の多層化学材料集積板、および
それを用いて多項目の化学分析を実施するための
液体試料の付着方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a sheet-like multilayer chemical analysis material accumulation plate and a method of using the same. The present invention relates to a multilayer chemical material accumulation plate having a structure in which the plates are arranged in contact with or close to each other, and a method for attaching liquid samples to the plate for carrying out multi-item chemical analysis.
液体試料を乾式で、迅速、簡易に化学定量分析
できる材料として、シート状の多層一体型化学定
量分析材料（以下、多層化学分析材料という。）
に関する発明が特開昭49−53888号、特開昭50−
137192号、特開昭51−40191号、特開昭52−3488
号、特開昭52−131786号、特公昭53−21677号、
米国特許第3526480号、同3663374号などの各明細
書および第10回国際臨床化学会（M′exico City、
1978年２月26日から３月３日）の発表予稿集
（13、14、47、76、118頁）などに記載されてい
る。 A sheet-shaped multilayer integrated chemical quantitative analysis material (hereinafter referred to as multilayer chemical analysis material) is used as a material for dry, quick, and easy chemical quantitative analysis of liquid samples.
The invention related to this is disclosed in Japanese Patent Application Laid-open No. 49-53888 and Japanese Patent Application Publication No. 50-53.
No. 137192, JP-A-51-40191, JP-A-52-3488
No., JP-A-52-131786, JP-A-53-21677,
Specifications such as U.S. Patent Nos. 3,526,480 and 3,663,374 and the 10th International Conference of Clinical Chemistry
It is described in the presentation proceedings (pages 13, 14, 47, 76, 118) from February 26, 1978 to March 3, 1978.
かかる多層化学分析材料は、分析のための試薬
を予めゼラチンの如き結合剤の中に含有させて薄
層状にする考え方のもので、実際の化学分析に当
つて被検液試料を１滴シート上に滴下又は付着さ
せるだけでよいので、従来法に較べ、試薬溶液と
試験管とを一切必要としない乾式の化学分析材料
である。 Such multilayer chemical analysis materials are based on the idea that reagents for analysis are pre-contained in a binder such as gelatin to form a thin layer.During actual chemical analysis, one drop of a test liquid sample is placed on a sheet. It is a dry chemical analysis material that does not require any reagent solutions or test tubes, compared to conventional methods, since it only needs to be dropped or attached to the surface of the material.
これらの多層化学分析材料の基本構成は多孔性
展開層と試薬層との組合わせであるが、試薬層は
機能を分離して、第一試薬層の下に第二試薬層又
発色層又は検出層又は色素受容層などと呼ばれる
複数層に構成することもある。又複数試薬層の中
間に光遮蔽層、バリヤ層などと呼ばれる中間層を
設けることもある。又、展開層中に試薬を包含さ
せて展開層と試薬層が合体された構造のものもあ
るが、いずれにしても、「展開層」と「試薬層」
の二つの作用層が基本である。 The basic composition of these multilayer chemical analysis materials is a combination of a porous development layer and a reagent layer, but the function of the reagent layer is separated, and a second reagent layer or coloring layer or detection layer is placed below the first reagent layer. It may be composed of multiple layers called a dye-receiving layer or a dye-receiving layer. Further, an intermediate layer called a light shielding layer, a barrier layer, etc. may be provided between the plurality of reagent layers. There are also structures in which the developing layer and reagent layer are combined by incorporating a reagent in the developing layer, but in any case, the "developing layer" and the "reagent layer"
There are two basic layers of action:
この多層化学分析材料上に滴下・付着された液
体試料は、多孔性展開層によつて一様に拡げられ
試薬層面に浸入する。試薬層中には、試薬液中の
ある成分と反応して終局的に発色、着色又は変色
反応に導く系が組込まれているので、終局試料液
中のある成分の含有量は色濃度に対応し、光学的
化学分析方法、主として比色法で定量することが
できるような仕組みになつている。 A liquid sample dropped and attached onto this multilayered chemical analysis material is uniformly spread by the porous spreading layer and penetrates into the reagent layer surface. The reagent layer contains a system that reacts with a certain component in the reagent solution to ultimately lead to color development, coloring, or color change reaction, so the final content of a certain component in the sample solution corresponds to the color concentration. However, the mechanism is such that it can be quantified using optical chemical analysis methods, primarily colorimetric methods.
多成分を含有する液体試料、たとえば、唾液、
血液、尿などの体液中のある化学成分を迅速、簡
易かつ試験管や溶液状試薬なしの乾式操作で化学
分析するためには、上記のシート状多層化学分析
材料は誠に好都合なものである。この材料は血中
のグルコース定量用、尿素窒素定量用、アミラー
ゼ定量用といつた様に、対象とする分析項目毎に
それ専用の分析材料が必要である。従つて、例え
ば上記３項目に加えてビリルビン、アルブミン、
コレステロール等の６項目の血中成分を定量しよ
うとする時は、まず６種類の多層化学分析材料を
選び出して用意し、つぎに全血又は血清の５μ
から15μを各材料上に１滴ずつ合計６回の滴下
付着操作が必要であり、続いて発色反応終了後、
各材料の順番をきめて、比色定量を行う。 Liquid samples containing multiple components, e.g. saliva,
The above-mentioned sheet-shaped multilayer chemical analysis material is very convenient for chemically analyzing certain chemical components in body fluids such as blood and urine quickly and easily using a dry operation without using test tubes or solution reagents. This material requires a dedicated analysis material for each target analysis item, such as for determining blood glucose, urea nitrogen, and amylase. Therefore, for example, in addition to the above three items, bilirubin, albumin,
When trying to quantify 6 blood components such as cholesterol, first select and prepare 6 types of multilayer chemical analysis materials, then prepare 5μ of whole blood or serum.
It is necessary to apply 1 drop of 15μ on each material for a total of 6 times, and then after the color reaction is completed,
Determine the order of each material and perform colorimetric determination.
上記の多層化学分析材料は定量の簡便さに関し
ては誠に優れたものであり、例えば血液化学分析
の目的で使用する時、患者１人１項目だけの分析
にはたいへん好都合である。しかし一般に行なわ
れている臨床化学検査では１人、１回の採血で５
から10項目の検査をするのが普通であり、その時
上記多層化学分析材料を利用するためには、まず
５から10種類の分析材料を１枚１枚選び出して、
順番づけし各材料上に血液を１滴ずつ枚数に応じ
て５回から10回滴下付着する方式で、かなり煩雑
な手順である。更に多人数について多項目の検査
を実施しようとすると、分析材料選び出し、順番
づけ、血液滴下の繰返しなど膨大な仕事量にな
る。そこでこの繰返し回数を最小限にするための
種々の実験を重ねた結果、予め必要な異種複数の
多層化学分析材料を支持板面上に特定の形に、例
えば放射状に配置して組込んだ構造の集積板を用
い、試料は各分析材料にまたがつて展開されるよ
うな点に付着させればよい事が判明して本発明に
到達した。 The above-mentioned multilayer chemical analysis material is excellent in terms of ease of quantitative determination, and is very convenient for analyzing only one item per patient, for example, when used for the purpose of blood chemistry analysis. However, in commonly performed clinical chemistry tests, one person can receive 50% in one blood draw.
Normally, 10 items are tested, and in order to use the multilayer chemical analysis materials mentioned above, first select 5 to 10 types of analysis materials one by one.
It is a fairly complicated procedure, in which one drop of blood is placed on each material in an orderly manner five to 10 times depending on the number of sheets. Furthermore, if a multi-item test is to be conducted on a large number of people, the amount of work involved will be enormous, such as selecting analysis materials, ordering them, and repeating blood dripping. As a result of various experiments to minimize the number of repetitions, we have developed a structure in which a plurality of required different types of multilayer chemical analysis materials are arranged in a specific shape, for example radially, on the surface of the support plate. The present invention was achieved based on the discovery that the sample can be attached to a point spread over each analysis material using an accumulating plate.
本発明の目的は、１個の化学分析用具と１回の
液体試料付着操作で３項目以上の化学定量分析を
実施できる多層化学分析材料集積板を提供するこ
とである。 An object of the present invention is to provide a multilayer chemical analysis material accumulation plate that can perform chemical quantitative analysis of three or more items using one chemical analysis tool and one liquid sample deposition operation.
本発明の他の目的は１個の化学分析用具と１回
の液体試料付着操作で３項目以上の化学定量分析
を同等の条件下で実施できる多層化学分析材料集
積板を提供することである。 Another object of the present invention is to provide a multilayer chemical analysis material stacking board that allows chemical quantitative analysis of three or more items to be performed under equivalent conditions using one chemical analysis tool and one liquid sample deposition operation.
本発明の他の目的は１個の化学分析用具と１回
の液体試料付着操作で３項目以上の化学定量分析
を実施できる多層化学分析材料集積板の使用方法
を提供することである。 Another object of the present invention is to provide a method for using a multilayer chemical analysis material accumulation plate that allows chemical quantitative analysis of three or more items to be performed using one chemical analysis tool and one liquid sample deposition operation.
本発明の他の目的は１個の化学分析用具と１回
の液体試料付着操作で３項目以上の化学定量分析
を同等の条件下で実施できる多層化学分析材料集
積板の使用方法を提供することである。 Another object of the present invention is to provide a method for using a multilayer chemical analysis material accumulation board that allows chemical quantitative analysis of three or more items to be performed under equivalent conditions using one chemical analysis tool and one liquid sample deposition operation. It is.
本発明の他の目的は１回の液体試料付着操作で
相異なる２種以上の多層化学分析材料に液体試料
を展開させて同じ条件下で各試薬層に液体試料を
到達させる方法を提供することである。 Another object of the present invention is to provide a method for spreading a liquid sample on two or more different types of multilayer chemical analysis materials in one liquid sample deposition operation and allowing the liquid sample to reach each reagent layer under the same conditions. It is.
本発明は、(1)支持板の上に各々試薬層と多孔性
展開層を有し、かつ互いに相異なる種類の３個以
上の多層化学分析材料が少なくとも該試薬層が互
いに接触又は近接するようにして配置されている
ことを特徴とする多層化学分析材料集積板ならび
に、(2)支持板の上に各々試薬層と多孔性展開層を
有し、かつ互いに相異なる種類の複数個の多層化
学分析材料が少なくとも該試薬層が互いに接触又
は近接するようにして配置されている多層化学分
析材料集積板の該展開層に液体試料を付着させ、
それにより該液体試料が該展開層により展開され
該試薬層に到達することを特徴とする多層化学分
析材料集積板の使用方法である。 The present invention provides: (1) three or more multilayer chemical analysis materials each having a reagent layer and a porous spreading layer on a support plate, and of different types, such that at least the reagent layers are in contact with or close to each other; (2) a plurality of multilayer chemical analysis materials of different types each having a reagent layer and a porous development layer on the support plate; attaching a liquid sample to the spreading layer of a multilayer chemical analysis material accumulation plate in which analysis materials are arranged such that at least the reagent layers are in contact with or close to each other;
This is a method of using a multilayer chemical analysis material accumulation plate, characterized in that the liquid sample is thereby spread out by the spreading layer and reaches the reagent layer.
本発明の要点である「試薬層が互いに接触又は
近接して配置されている」とは、多孔性展開層に
付着された液体試料が展開層により展開されて広
がり試薬層に到達しうる範囲内に試薬層（又は、
必要により試薬層と他の層）が少なくともその一
端が接触又は近接して配置されていることを意味
する。好ましくは支持板面上の一点（以下、中心
点という。）の上方の多孔性展開層に付着された
液体試料が展開層により展開されて広がり試薬層
に到達しうる範囲内に、光学的方法により化学分
析しうる広さの試薬層（化学分析を実施するため
に必要に応じて設けられる他の層をも含める。）
（以下、化学分析領域という。）が存在するように
試薬層（又は試薬層と他の層）が互いに少なくと
もその一端が接触又は近接して配置されているこ
とを意味する。近接して配置するとは、第２図に
示し後述するように、試薬層（又は試薬層と他の
層）の最も近い部分相互を約0.5mmから約５mmの
間隙をあけて配置することである。ここで多孔性
展開層が一つの連続した実質的にいたる所で等し
い構造を有する層である場合には、中心点の上に
付着された液体試料が展開層により展開されて広
がつた先端を結んだ線は、おおよそ中心点を中心
とする閉じた円又は円に近似する図形（以下、両
者をあわせて展開円という。）の周を形成する。
また一方で、液体試料の付着量を多くすれば、そ
の量に比例して展開円はほとんど制限なしに大き
くなり、その展開円の内部では液体試料が試薬層
に到達するが、展開円の直径にほぼ比例して液体
試料が展開されるのに要する時間が長くなる。し
かし本発明の分析材料集積板を用いて化学分析す
るに際しては、主として操作の容易さと、一連の
操作（液体試料の適用から化学分析の完了まで）
に要する時間をなるべく短くするという要請によ
り制約されるので、付着される液体試料の量が少
量に制限されることが多い。このような実用上の
理由により、展開円の半径は約３mmから約30mm、
好ましくは約３mmから約20mmの範囲が選択され、
一方、多層化学分析材料の分析領域は展開円の内
部に存在しなければならない。従つて、多層化学
分析材料は中心点を中心にして半径約30mm以下、
好ましくは半径約20mm以下の円内にその分析領域
が存在するように配置することができる。 The gist of the present invention, "the reagent layers are arranged in contact with or close to each other" means that the liquid sample attached to the porous development layer is spread out by the development layer and can reach the reagent layer. reagent layer (or
This means that at least one end of the reagent layer and other layers (if necessary) are in contact with or are placed in close proximity. Preferably, the liquid sample attached to the porous developing layer above a point (hereinafter referred to as the center point) on the surface of the support plate is spread by the developing layer and reaches the reagent layer using an optical method. reagent layer of a size that allows chemical analysis (including other layers provided as necessary to perform chemical analysis)
(hereinafter referred to as a chemical analysis region) means that the reagent layers (or the reagent layer and other layers) are arranged so that at least one end thereof is in contact with or close to each other so that there is a chemical analysis region. Closely located means that the closest parts of the reagent layer (or reagent layer and other layers) are spaced apart from each other by about 0.5 mm to about 5 mm, as shown in FIG. 2 and described below. . If the porous spreading layer is one continuous layer having substantially the same structure everywhere, the liquid sample deposited on the central point is spread out by the spreading layer to form a widened tip. The connected lines form the periphery of a closed circle centered approximately at the center point or a figure approximating a circle (hereinafter, both are collectively referred to as an expanded circle).
On the other hand, if the amount of liquid sample attached is increased, the development circle will increase in proportion to the amount without any limit, and the liquid sample will reach the reagent layer inside the development circle, but the diameter of the development circle will increase. The time required for the liquid sample to develop increases approximately in proportion to However, when performing chemical analysis using the analytical material accumulating plate of the present invention, the main considerations are ease of operation and a series of operations (from application of liquid sample to completion of chemical analysis).
The amount of liquid sample deposited is often limited to a small amount, constrained by the desire to minimize the amount of time required for this process. For these practical reasons, the radius of the development circle is approximately 3 mm to approximately 30 mm,
Preferably a range of about 3 mm to about 20 mm is selected;
On the other hand, the analysis region of the multilayer chemical analysis material must exist inside the development circle. Therefore, the multilayer chemical analysis material has a radius of about 30 mm or less around the center point.
Preferably, the analysis area can be arranged within a circle with a radius of about 20 mm or less.
異種の３個以上の特定の形の多層化学分析材料
の配置の具体例は、扇形の頂点、三角形、菱形、
長方形、正方形等の多角形の１頂点を中心点で互
いに接触させた、又は中心点を中心とする一定の
半径の円に内接させた放射状配置、多角形、円、
楕円形、卵円形を中心点を中心とする一定の半径
の円に内接させた配置、中心点を中心とする一定
の半径の円内におさめた格子縞状の配置、中心点
を中心とする一定の半径の円内に不規則に並べた
配置をあげることができる。ここで前述の一定の
半径の円は展開円に等しいか小さい円である。と
ころで、多孔性展開層により展開された液体試料
は、その付着された中心点から等しい距離の点で
は、特に均一性（単位面積当りの液体試料の量が
等しいこと。）よく試薬層に到達するので、前二
者の配置が好ましい。 Specific examples of the arrangement of three or more different specific shapes of multilayer chemical analysis materials include fan-shaped vertices, triangles, diamonds,
A radial arrangement, a polygon, a circle, in which one vertex of a polygon such as a rectangle or square is in contact with each other at the center point, or inscribed in a circle of a constant radius centered on the center point,
An ellipse, an arrangement in which an oval shape is inscribed in a circle of a certain radius centered on the center point, a plaid arrangement in a circle of a certain radius centered on the center point, and an arrangement centered on the center point It can be arranged irregularly within a circle with a fixed radius. Here, the aforementioned circle of constant radius is a circle that is equal to or smaller than the developed circle. By the way, the liquid sample spread by the porous spreading layer has good uniformity (equal amount of liquid sample per unit area) at points at equal distances from the center point to which it is attached, so that it reaches the reagent layer. Therefore, the former two arrangements are preferable.
本発明の多層化学分析材料集積板に配置する多
層化学分析材料の数は、３個以上であり、好まし
くは４個以上であるが、その数は多いほぼ一般的
には好ましい。 The number of multilayer chemical analysis materials disposed on the multilayer chemical analysis material accumulation board of the present invention is three or more, preferably four or more, but a large number is generally preferred.
支持板は、ポリエステル（例、ポリエチレンテ
レフタレート、ビスフエノールのポリカーボネー
ト）、セルロースエステル（例、セルロースジア
セテート、セルローストリアセテート、セルロー
スアセテートプロピオネート、セルロースアセテ
ートブチレート、セルロースニトレート）、セル
フアン、ポリスチレン、ポリオレフイン（例、ポ
リエチレン、ポリプロピレン）、ハロゲン含有ビ
ニルポリマー（例、ポリ塩化ビニル、ポリ塩化ビ
ニリデン）、有機または無機のガラスの近紫外
部、可視光部、近赤外部にかけて透明性のよい材
料でできた厚さ約10μｍから約0.5mm、好ましく
は約20μｍから約0.3mmまでの板状物又はその積
層物を用いることができる。支持板にはその周辺
に全辺にわたつて、又はその一部に枠を設けるこ
とができる。また、支持板としてシリコーン樹脂
等の離型剤をごくうすく塗布した前述の支持板又
は他の支持体（紙、プラスチツク、金属箔等の半
透明又は不透明な支持体をも用いることができ
る。）を用いると、保護の役目をし、測定の際に
はこれを剥がして除去してから測定することがで
きる。剥して除去できる支持板を用いる場合に
は、集積板の構造を支えるために各多層化学分析
材料に共通した一つの多孔性展開層を設けるのが
好ましい。 The support plate can be made of polyester (e.g., polyethylene terephthalate, polycarbonate of bisphenol), cellulose ester (e.g., cellulose diacetate, cellulose triacetate, cellulose acetate propionate, cellulose acetate butyrate, cellulose nitrate), cellulose, polystyrene, polyolefin. (e.g., polyethylene, polypropylene), halogen-containing vinyl polymers (e.g., polyvinyl chloride, polyvinylidene chloride), organic or inorganic glass materials that are highly transparent in the near-ultraviolet, visible, and near-infrared regions. A plate or a laminate thereof having a thickness of about 10 μm to about 0.5 mm, preferably about 20 μm to about 0.3 mm can be used. The supporting plate can be provided with a frame around the entire side or a part thereof. Further, as a support plate, the above-mentioned support plate coated with a very thin release agent such as silicone resin or other supports (translucent or opaque supports such as paper, plastic, metal foil, etc. can also be used). When used, it serves as a protection, and can be peeled off and removed before measurement. If a peelable and removable support plate is used, it is preferable to provide one porous spread layer common to each multilayer chemical analysis material to support the structure of the stack plate.
支持体、試薬層、また試薬層の機能を分離する
場合の第一試薬層、第二試薬層、発色層、検出
層、色素受容層、色遮蔽層、バリヤ層などの構
成、調製方法および多層化学分析材料への組込み
方法は前述の明細書の記載に従つて実施すること
ができる。とくに色遮蔽層としては多孔性の金属
膜からなる水浸透性の色遮蔽層又は金属粉末を含
む水浸透性の色遮蔽層が好ましく、これらの色遮
蔽層の構成、調製方法、多層化学分析材料への組
込み方法については特開昭55−26428及び特開昭
55−26429各明細書の記載に従つて実施すること
ができる。また、支持体は省略することができ、
この場合には支持板が個々の多層化学分析材料の
支持体も兼ねる。 Structures, preparation methods, and multilayers of the support, reagent layer, first reagent layer, second reagent layer, coloring layer, detection layer, dye-receiving layer, color-shielding layer, barrier layer, etc. when separating the functions of the reagent layer The method for incorporating it into chemical analysis materials can be carried out as described in the above specification. In particular, the color shielding layer is preferably a water-permeable color shielding layer made of a porous metal film or a water-permeable color shielding layer containing metal powder, and the composition, preparation method, and multilayer chemical analysis material of these color shielding layers are preferably used. For information on how to incorporate
55-26429 can be carried out according to the descriptions in each specification. Also, the support can be omitted,
In this case, the support plate also serves as a support for the individual multilayer chemical analysis materials.
本発明の多層化学分析材料集積板の基本的形態
は、第１図に示した断面図の如く、支持板１、支
持板上のある点（中心点）２を中心に支持板面上
に各試薬層が互いに接触又は近接して配置された
異種複数の試薬層３１，３２及び多孔性展開層４
０の三者が積層されて固定された形成のものであ
る。各試薬層３１，３２は点２を境にして相接触
していてもよく、又ある間隙をもつて離れて（す
なわち近接していて）いてもよい。多孔性展開層
４０は各試薬層３１，３２に共通の一枚物でもよ
く、又各試薬層のそれぞれに固有の複数のもので
あつてもよいが、多孔性展開層は共通の一枚物を
用いることが好ましい場合もある。これは、液体
試料が多孔性展開層により展開される際に、展開
層が一枚物であれば液体試料の展開が実質的にい
たる所で均一に起るので、化学分析の精度を高め
ることができるからである。ここで試薬層と多孔
性展開とが組合つて始めて一種の多層化学分析材
料を形成するので、本発明の多層化学分析材料集
積板は異種複数の化学分析材料が支持板面上に接
触又は近接して配置された構造であると表現する
方が理解し易い。 The basic form of the multilayer chemical analysis material accumulation board of the present invention is as shown in the cross-sectional view shown in FIG. A plurality of different reagent layers 31 and 32 and a porous development layer 4 in which the reagent layers are arranged in contact with or close to each other.
0 are laminated and fixed. The reagent layers 31 and 32 may be in contact with each other with the point 2 as a boundary, or may be separated (that is, close to each other) with a certain gap. The porous spreading layer 40 may be a single piece common to each reagent layer 31, 32, or may be a plurality of pieces unique to each reagent layer, but the porous spreading layer 40 may be a single piece common to each reagent layer 31, 32. In some cases, it may be preferable to use This is because when a liquid sample is developed by a porous developing layer, if the developing layer is made of a single piece, the development of the liquid sample occurs substantially uniformly everywhere, which improves the precision of chemical analysis. This is because it can be done. Since the reagent layer and the porous expansion form a kind of multilayer chemical analysis material only when they are combined, the multilayer chemical analysis material accumulation plate of the present invention allows a plurality of different chemical analysis materials to contact or be close to each other on the surface of the support plate. It is easier to understand if it is expressed as a structure arranged as follows.
多孔性展開層の上（試薬層から遠い方の表面の
上）には、実開昭54−162295号明細書に記載され
ている少なくとも１個の小孔を有する防水性層、
実開昭54−162293号又は実開昭54−162294号明細
書に記載されている水分蒸発防止カバー、又は実
開昭54−178495号明細書に記載されている血液中
の有形成分を過する過層と少なくとも１個の
小孔を有する防水性層（防水性層が多孔性展開層
に接して設けられる。）の組合せを設けることが
できる。 On the porous spreading layer (on the surface far from the reagent layer), there is a waterproof layer having at least one small pore as described in Japanese Utility Model Application No. 162295/1983;
The cover for preventing moisture evaporation described in the specification of Japanese Utility Model Application No. 54-162293 or 1984-162294, or the cover that prevents the formation of substances in the blood described in the specification of the Japanese utility model application publication No. 54-178495. A combination of a waterproof layer having at least one small pore (the waterproof layer being provided in contact with the porous deployment layer) can be provided.
異種複数の多層化学分析材料を支持板上に形成
する方法は、既成の相異なる多層化学分析材料を
円形、扇形又は多角形にカツトしたものを支持板
面上に配置し接着する切貼り法をとることもでき
るし、また支持板上に、スポツト塗布法や切貼り
法等でまず複数の相異なる試薬層を形成した後、
多孔性展開層を積層接着する方法をとることもで
きるなど、実際の形成法は分析項目数、製造量、
コスト等を考慮して任意に選択することができ
る。また、異種複数の多層化学分析材料のうち、
一種のみを、扇形の場合にはその中心角を大きく
または小さく作り、円形又は多角形の場合には大
きさ又は形を変えることにより、集積板に使用す
る際の向き、あるいは集積板に配列された多層化
学分析材料の識別をするための手段とすることが
できる。 A method for forming a plurality of different types of multilayer chemical analysis materials on a support plate is a cut-and-paste method in which different multilayer chemical analysis materials are cut into circular, fan-shaped, or polygonal shapes and placed on the surface of the support plate and adhered. Alternatively, after first forming a plurality of different reagent layers on a support plate using a spot coating method or a cutting and pasting method,
The actual formation method depends on the number of analysis items, production volume,
It can be arbitrarily selected in consideration of cost and the like. In addition, among the multilayered chemical analysis materials of different types,
By making only one type with a larger or smaller central angle in the case of a fan shape, or by changing the size or shape in the case of a circular or polygonal shape, it is possible to change the orientation when used on the stacking board or how to arrange it on the stacking board. It can be used as a means for identifying multilayer chemical analysis materials.
次に本発明の多層化学分析材料集積板の使用方
法について説明する。まず第一に液体試料を多孔
性展開層の上に付着させる。付着させる位置は中
心点または中心点の近傍の多孔性展開層の上面で
ある。付着させる方法は具体的には液体試料の滴
下、液体試料のおしつけ又は塗りつけ、液体試料
の吹きつけ等をあげることができる。なお、本明
細書においては、これらのすべての付着させる方
法およびそのプロセスだけでなく、多孔性展開層
の上に他の層が設けられている場合のように間接
的に多孔性展開層に液体試料を付着させる方法お
よびそのプロセスをも含めて付着（する）とい
う。本発明の集積板に少なくとも１個の小孔を有
する防水層又は過層が最上層として設けられて
いる場合には、液体試料は防水性層の小孔部分又
は中心点の上方の過層に付着させることによ
り、液体試料が小孔を通過して、又は過層を通
過して（有形成分が含まれている場合には有形成
分が過されて）多孔性展開層に付着することが
できる。さらに詳細に具体的に説明すると、液体
試料１回の適用で、支持板上に配置された相異な
る種類の複数個の多層化学分析材料各々の少なく
とも試薬層には多孔性展開層により展開された液
体試料がほぼ均一にゆきわたるよう、中心点（第
１図の点２）又は中心点の近傍の上方にＳ方向か
ら液体試料を付着する。液体試料の付着は１回で
十分である。このようにして付着された液体試料
は各々の多層化学分析材料の個々の多孔性展開
層、又は共通の一つの多孔性展開層によつて展開
されて各試薬層に到達し、ついで各試薬層内にゆ
きわたる。そこで液体試料は各試薬層に含有され
る各試薬と反応して発色又は変色（以下、あわせ
て発色という。）する。発色反応が実質的に終了
した後に、各発色濃度は、第１図のV1，V2の方
向から順に測定して液体試料中の化学成分を定量
することができる。ここまでの説明で明らかにな
つたとおり、本発明の集積板を用いて液体試料を
１回だけ付着する使用方法は、化学分析の観点か
らは、各多層化学分析材料（より具体的には各試
薬層）への液体試料の付着または到達がすべて同
等の条件下で実施されるという著しい特徴があ
り、このことは各化学分析材料における定量に必
然的に付随する誤差の要因が実質的に除去され
る、あるいは著しく減少されることを意味するこ
とになる。 Next, a method of using the multilayer chemical analysis material stacking board of the present invention will be explained. First, a liquid sample is deposited onto the porous spreading layer. The position of attachment is the upper surface of the porous spreading layer at or near the center point. Specific methods for adhesion include dropping a liquid sample, basting or smearing a liquid sample, and spraying a liquid sample. Note that this specification describes not only all of these deposition methods and processes, but also the application of liquid to the porous deployant layer indirectly, such as when other layers are provided on the porous deployant layer. Attachment also includes the method and process of attaching a sample. If the accumulation plate of the present invention is provided with a waterproofing layer or superlayer having at least one small hole as the top layer, the liquid sample is applied to the small hole area of the waterproofing layer or the superlayer above the central point. By depositing, the liquid sample passes through the small pores or through the overlayer (if it contains a solid component, the solid component is passed through) and deposits on the porous spreading layer. I can do it. More specifically, in one application of a liquid sample, at least the reagent layer of each of a plurality of different types of multilayer chemical analysis materials arranged on a support plate is developed by a porous development layer. The liquid sample is deposited from the S direction above the center point (point 2 in FIG. 1) or near the center point so that the liquid sample is spread almost uniformly. It is sufficient to attach the liquid sample once. The liquid sample deposited in this manner is spread out by the individual porous spreading layers of each multilayered chemical analysis material or by a common porous spreading layer to reach each reagent layer, and then to each reagent layer. Spread within. Therefore, the liquid sample reacts with each reagent contained in each reagent layer to develop or change color (hereinafter collectively referred to as color development). After the color reaction is substantially completed, each color concentration can be measured in order from the directions V 1 and V 2 in FIG. 1 to quantify the chemical components in the liquid sample. As has been made clear from the explanation up to this point, the usage method of depositing a liquid sample only once using the stacking plate of the present invention is not suitable for each multilayer chemical analysis material (more specifically, for each multilayer chemical analysis material) from the viewpoint of chemical analysis. It has the remarkable feature that the deposition or access of the liquid sample to the reagent layer (reagent layer) is all carried out under comparable conditions, which virtually eliminates the sources of error that inevitably accompany the quantification of each chemical analysis material. This may mean that the amount is increased or significantly reduced.
本発明の多層化学分析材料集積板の使用方法
は、一つの支持体の上に互いに相異る２種以上の
試薬層と共通の又は個別の多孔性展開層を設けた
多層化学分析材料の使用方法にまで拡張すること
ができる。すなわち、本発明の集積板のごとくは
じめから３項目以上の化学分析を１回の液体試料
の付着で実施することを意図した分析材料ではな
いが、多層化学分析材料の試薬層と多孔性展開層
の分析領域の配置が展開円の内部にあるような多
層化学分析材料である場合には、本発明の方法を
その材料にまで拡張して実施することができる。
とくに相異る２種の試薬層が接触又は近接して設
けられている多層化学分析材料においては、２種
の試薬層の配置の中心点、２種の試薬層の接触部
分の一点等、２種の試薬層における各分析領域間
の対称中心点またはその近傍の多孔性展開層の上
に液体試料を付着させることにより本発明の方法
を実施することができる。 A method of using the multilayer chemical analysis material accumulation plate of the present invention is to use a multilayer chemical analysis material in which two or more different reagent layers and a common or individual porous development layer are provided on one support. It can be extended to methods. In other words, although it is not an analysis material intended to perform chemical analysis of three or more items in one application of a liquid sample like the stacking plate of the present invention, it is a multilayer chemical analysis material with a reagent layer and a porous spreading layer. In the case of a multilayered chemical analysis material in which the analysis region is located inside the development circle, the method of the present invention can be extended to that material.
In particular, in a multilayer chemical analysis material in which two different types of reagent layers are provided in contact with or close to each other, the center point of the arrangement of the two types of reagent layers, one point in the contact area between the two types of reagent layers, The method of the present invention can be practiced by depositing a liquid sample onto the porous spreading layer at or near the center of symmetry between each analysis area in the seed reagent layer.
本発明の多層化学分析材料集積板の具体例を第
２図から第５図に亘つて模型的に示してある。 Specific examples of the multilayer chemical analysis material stacking board of the present invention are schematically shown in FIGS. 2 to 5.
第２図と第３図は試薬層と多孔性展開層とより
なる完成した多層化学分析材料の６種類のもの
を、扇形状に切つて中心点の近傍に、その一端が
くるように放射状に近接させて配置一体化した構
造の例である。第２図はこれを上から眺めた図
で、透明支持板１上の中心点２を中心に、台形又
は扇形状に切つた、６種類の完成した多層化学分
析材料６１から６６を放射状に配置して接着し、
各分析材料の一端は、すべて中心点２を中心とす
る半径４mmの円周に内接して存在するようにして
ある。６種の分析材料は隙間なく並べることもで
きるし、図示の如く0.5mmから５mmの間隙８をあ
けることもできる。各分析材料の間隙及び放射状
配置の中心部分を防水、撥水加工することができ
る。 Figures 2 and 3 show six types of completed multilayer chemical analysis materials consisting of a reagent layer and a porous spreading layer, cut into fan shapes and radially arranged so that one end is near the center point. This is an example of a structure in which they are placed close together and integrated. FIG. 2 shows this as seen from above, and six types of completed multilayer chemical analysis materials 61 to 66 cut into trapezoids or fan shapes are arranged radially around the center point 2 on the transparent support plate 1. and glue,
One end of each analysis material is inscribed in a circumference having a radius of 4 mm centered on the center point 2. The six types of analysis materials can be arranged without any gaps, or they can be arranged with a gap 8 of 0.5 mm to 5 mm as shown in the figure. The gaps and the central portions of the radial arrangement of each analysis material can be treated to be waterproof and water repellent.
第３図は第２図の構造の多層化学分析材料集積
板をX2−Y2の破線にそつて切断した断面の模型
図である。透明支持板１の上に異種の分析材料６
１，６４が中心点２を中心に配置されている。分
析材料６１，６４はそれぞれ透明支持体５１，５
４試薬層３１，３４多孔性展開層４１，４４から
なることを例示した。透明支持体５１，５４は省
略することも可能である。中心点上にＳ方向から
50〜90μの液体試料を付着させる。試薬層中の
発色濃度はV1，V4の方向から各材料について順
に比色定量にする。支持板は平面が原則である
が、中心点２を中心に山型やすり鉢型になつてい
てもよい。支持板材料は光透過性のよい有機又は
無機ガラス類や写真用フイルムベースがもつとも
好ましい。又単なる軟質透明フイルムや又は枠つ
きの薄い軟質透明フイルムをも利用することがで
きるし、支持板は展開層側にもつてくることも可
能であるが、この場合試料付着はＳ方向と反対の
方向から行う。 FIG. 3 is a cross-sectional model diagram of the multilayer chemical analysis material stacking plate having the structure shown in FIG. 2, cut along the broken line X 2 -Y 2 . Different types of analysis materials 6 on transparent support plate 1
1 and 64 are arranged around center point 2. Analysis materials 61 and 64 are transparent supports 51 and 5, respectively.
The example is shown as consisting of four reagent layers 31, 34 and porous spreading layers 41, 44. It is also possible to omit the transparent supports 51 and 54. From the S direction on the center point
Deposit 50-90μ of liquid sample. The color concentration in the reagent layer is determined colorimetrically for each material in order from the direction of V 1 and V 4 . In principle, the support plate is flat, but it may also be shaped like a chevron or a mortar around the center point 2. The supporting plate material is preferably an organic or inorganic glass or a photographic film base having good light transmittance. Also, a simple flexible transparent film or a thin flexible transparent film with a frame can be used, and the support plate can also be placed on the developing layer side, but in this case, the sample should be attached in the opposite direction to the S direction. Start from
第４図、第５図は展開層をまだ積層していない
段階の、即ち試薬層又は試薬層と支持体だけから
なる未完成の多層化学分析材料を三角形状に、塗
布するか又は切貼りする方法で、中心点を中心に
放射状に接触させて配置して一体化し、その後円
形の多孔性展開層をその中心を中心点に一致させ
て積層し固定して一体化した形態のものを示す。
第４図はその平面図で、まず支持板１の上の中心
点２を中心に、正三角形状に切つた６種類の未完
成の多層化学分析材料７１から７６の頂点を互い
に接触させて放射状に配置固定する。次に中心点
２を中心に半径約1.5〜４cmの円形または多角形
の一つの多孔性展開層４９を積層接着し６個の多
層化学分析材料に共通の多孔性展開層を形成させ
て多層化学分析材料を完成すると同時に集積板が
でき上る。 Figures 4 and 5 show a state in which the developing layer has not yet been laminated, that is, an unfinished multilayer chemical analysis material consisting only of a reagent layer or a reagent layer and a support is coated or cut and pasted in a triangular shape. In this method, the materials are integrated by arranging them in radial contact around the center point, and then the circular porous spread layers are laminated with their centers aligned with the center point and fixed to form an integrated structure.
FIG. 4 is a plan view of the same. First, centering on the center point 2 on the support plate 1, the vertices of six types of unfinished multilayer chemical analysis materials 71 to 76 cut into equilateral triangles are brought into contact with each other and radially arranged. Place and fix. Next, one circular or polygonal porous spread layer 49 with a radius of approximately 1.5 to 4 cm is laminated and bonded around the center point 2 to form a common porous spread layer among the six multilayer chemical analysis materials. At the same time as the analytical materials are completed, the stacking board is completed.
第５図は上記第４図に示した構造のもののX4
−Y4破線に沿つた断面の模型図である。 Figure 5 shows the X 4 structure shown in Figure 4 above.
-Y is a cross-sectional model diagram taken along the broken line 4 .
支持板１の上に、透明支持体５１，５４及び試
薬層３１，３４からなる（多孔性展開層を有しな
い）未完成の多層化学分析材料７１，７４があ
り、その上に両者に共通の多孔性展開層４９を積
層接着して、多層化学分析材料とその集積板を完
成したものである。 On the support plate 1, there are unfinished multilayer chemical analysis materials 71, 74 (without a porous development layer) consisting of transparent supports 51, 54 and reagent layers 31, 34, and on top of them are A porous spread layer 49 is laminated and bonded to complete a multilayer chemical analysis material and its integrated plate.
液体試料の付着はＳ方向から、比色定量は
V1、V4方向から行うことは第２図の集積板と同
様である。ここで、各分析材料の支持体５１，５
４を省略した形式、即ち共通の支持板と共通の多
孔性展開層の間に、各種の三角形状試薬層が放射
状に接触して配置された形式のものにすることも
できる。更に第５図において、支持板１を、その
位置からはずし、展開層４９の上方においた構造
も可能である。このとき支持板１には液体試料付
着のための適当な小孔が中心点２の上方に必要で
ある。 The liquid sample is attached from the S direction, and the colorimetric determination is
The process from the V 1 and V 4 directions is the same as the stacking board shown in FIG. 2. Here, supports 51 and 5 of each analysis material
4 may be omitted, that is, various triangular reagent layers may be arranged in radial contact between a common support plate and a common porous spreading layer. Furthermore, in FIG. 5, it is also possible to remove the support plate 1 from that position and place it above the spreading layer 49. At this time, the support plate 1 needs to have a suitable small hole above the center point 2 for the attachment of the liquid sample.
第５図、第６図は別の多層化学分析材料集積板
の例を示す。この例においては、支持板１の上に
円形の８種の試薬層７１から７８が、中心点２を
中心とする特定の半径の円に内接するようにして
互いに間隙をおいて配置され、その上に一つの共
通の円形の多孔性展開層４９を設けられている。
第６図はこの集積板の平面図、第７図は第６図に
おけるX6−Y6破線にそつた断面の模型図であ
る。液体試料の付着はＳ方向から中心点２の上方
の多孔性展開層４９の上に行い、比色定量は
V1、V5方向から行う。 FIGS. 5 and 6 show another example of a multilayer chemical analysis material accumulation board. In this example, eight circular reagent layers 71 to 78 are arranged on the support plate 1 with gaps between them so as to be inscribed in a circle of a specific radius centered on the center point 2. A common circular porous spreading layer 49 is provided on top.
FIG. 6 is a plan view of this stacking board, and FIG. 7 is a cross-sectional model diagram taken along the broken line X 6 -Y 6 in FIG. 6. The liquid sample is deposited on the porous development layer 49 above the center point 2 from the S direction, and the colorimetric determination is
Perform from the V 1 and V 5 directions.
ここまでの説明では、多層化学分析材料６種又
は８種を用いて集積板を構成する例を用いて説明
したが、多層化学分析材料を３種以上用いて本発
明の集積板を構成することができることはいうま
でもない。 Up to this point, the explanation has been given using an example in which an integrated board is constructed using six or eight types of multilayer chemical analysis materials, but the integrated board of the present invention may be constructed using three or more types of multilayer chemical analysis materials. Needless to say, it can be done.
本発明の多層化学分析材料集積板の特徴を従来
の多層化学分析材料と比較して述べる。 The features of the multilayer chemical analysis material stacking board of the present invention will be described in comparison with conventional multilayer chemical analysis materials.
(1) 従来の多層化学分析材料は分析項目１項目当
り１枚の分析材料を必要とするので、多項目分
析に当つては多数枚の分析材料を選び出し、順
序づけする必要があつた。本発明の集積板は１
枚の支持板に予め必要項目の分析材料の複数枚
を組にして配列してあるので、分析材料の選び
出しと順番づけする手間が省け、かつ貯蔵空間
が節約できるコンパクト型である。(1) Conventional multilayer chemical analysis materials require one analysis material for each analysis item, so it is necessary to select and order a large number of analysis materials for multi-item analysis. The integrated board of the present invention has 1
Since a plurality of sheets of analytical materials for necessary items are arranged in sets on one support plate in advance, it is a compact type that saves the trouble of selecting and ordering the analytical materials and saves storage space.
(2) 従来の多層化学分析材料は１項目の分析毎に
１回ずつ試料を滴下・付着する操作が必要であ
るため、分析項目数に応じた多数回の試料滴下
が必要であつたが、本発明の集積板は１回だけ
の試料付着操作で多項目の分析が可能であるの
で、分析操作がより一層簡略化され、かつ操作
ミスの入る余地が少く、かつ試料付着操作を自
動化しようとするとき、従来のものに比較し数
段簡易なものとなる。(2) Conventional multilayer chemical analysis materials require the operation of dropping and depositing the sample once for each analysis item, so it is necessary to drop the sample multiple times depending on the number of analysis items. Since the accumulating plate of the present invention enables analysis of multiple items with only one sample attachment operation, the analysis operation is further simplified, there is less room for operational errors, and it is possible to automate the sample attachment operation. When doing so, it becomes much simpler than the conventional method.
(3) 特に血液化学分析の分野で本発明の集積板を
利用する場合、緊急検査に要な複数項目分析用
の多層化学分析材料を一定順序で接触又は近接
して配置固定したシートを用意しておくと、１
枚のシートと１回の血液付着だけで必要項目の
検査が短時間にできるので、たいへん有用な緊
急検査用の分析手段を提供するものとなる。(3) In particular, when using the accumulating plate of the present invention in the field of blood chemistry analysis, a sheet is prepared in which multilayer chemical analysis materials for multiple-item analysis required for emergency testing are arranged and fixed in contact or close together in a certain order. If you keep it, 1
Since the required items can be tested in a short time with only one sheet and one blood deposition, it provides a very useful analytical means for emergency testing.
第１図は本発明の多層化学分析材料集積板の基
本的形態の断面図である。第２図から第５図は本
発明の多層化学分析材料集積板の具体例模型図で
ある。第２図は完成した多層化学分析材料の異種
のもの６個を支持板面上の中心点を中心とする小
円に外接するよう放射状に近接して配置した形式
の多層化学分析材料集積板であり、第３図はX2
−Y2破線に沿つた断面図である。第４図は多孔
性展開層を有しない未完成の多層化学分析材料の
異種のもの６個を支持板面上の中心点を中心に放
射状に接触させて配置した後共通の展開層一枚を
積層した形式の多層化学分析材料板であり、第５
図はそのX4−Y4破線に沿つた断面図である。第
６図は８個の異種の円形の試薬層を支持板面上の
中心点を中心とする特定の半径の円に内接して配
置した後共通の円形の展開層を積層した多層化学
分析材料集積板であり、第７図はそのX6−Y6破
線に沿つた断面図である。
１：支持板、２：中心点、３１，３２，３４，
３５：試薬層、４０：多孔性展開層、４１，４
４：個々の多孔性展開層、４９：共通の多孔性展
開層、５１，５４：個々の透明支持体、６１，６
２，６３，６４，６５，６６：個々の多孔性展開
層を有する異種の多層化学分析材料、７１，７
２，７３，７４，７５，７６，７７，７８：個々
の多孔性展開層を有しない異種の多層化学分析材
料、８：多層化学分析材料間の間隙、Ｓ：液体試
料付着方向、V1，V2，V4，V5：比色定量方向、
X2−Y2，X4−Y4，X6−Y6：多層化学分析材料集
積板の切断面を示す破線。
FIG. 1 is a sectional view of the basic form of the multilayer chemical analysis material stacking plate of the present invention. FIGS. 2 to 5 are schematic diagrams of specific examples of the multilayer chemical analysis material stacking board of the present invention. Figure 2 shows a multilayer chemical analysis material accumulation board in which six different types of completed multilayer chemical analysis materials are arranged radially close to each other so as to circumscribe a small circle centered on the center point on the surface of the support plate. Yes, Figure 3 is X 2
-Y2 is a cross-sectional view along the broken line. Figure 4 shows six different types of unfinished multilayer chemical analysis materials that do not have a porous spread layer placed in radial contact around the center point on the surface of the support plate, and then one common spread layer. It is a multilayer chemical analysis material plate in a laminated format, and the fifth
The figure is a sectional view taken along the X 4 -Y 4 broken line. Figure 6 shows a multilayer chemical analysis material in which eight different circular reagent layers are arranged inscribed in a circle with a specific radius centered on the center point on the support plate surface, and then a common circular developed layer is laminated. It is an integrated board, and FIG. 7 is a sectional view thereof along the X 6 -Y 6 broken line. 1: Support plate, 2: Center point, 31, 32, 34,
35: Reagent layer, 40: Porous development layer, 41, 4
4: Individual porous spreading layer, 49: Common porous spreading layer, 51, 54: Individual transparent support, 61, 6
2, 63, 64, 65, 66: Heterogeneous multilayer chemical analysis materials with individual porous spread layers, 71, 7
2, 73, 74, 75, 76, 77, 78: Different types of multilayer chemical analysis materials without individual porous spread layers, 8: Gaps between multilayer chemical analysis materials, S: Liquid sample deposition direction, V 1 , V 2 , V 4 , V 5 : Colorimetric direction,
X 2 −Y 2 , X 4 −Y 4 , X 6 −Y 6 : Broken lines indicating the cut surface of the multilayer chemical analysis material accumulation board.
Claims (1)
し、かつ互いに相異なる種類の３個以上の多層化
学分析材料が設けられてなり、該試薬層は互いに
接触又は0.5mmから５mmの間隙で近接して配置さ
れ、該展開層は該試薬層と同一の形状で該試薬層
ごとに設けられているか、又は該展開層は展開円
より大きい形状で該試薬層全てに共通に１個設け
られていることを特徴とする多層化学分析材料集
積板。 ２ 支持板の上に各々試薬層と多孔性展開層を有
し、かつ互いに相異なる種類の複数個の多層化学
分析材料が設けられてなり、該試薬層が互いに接
触又は0.5mmから５mmの間隙で近接して配置さ
れ、該展開層は該試薬層と同一の形状で該試薬層
ごとに設けられているか、又は該展開層は展開円
より大きい形状で該試薬層全てに共通に１個設け
られている多層化学分析材料集積板の該展開層に
液体試料を付着させ、それにより該液体試料が該
展開層により展開され該複数個の試薬層各々に到
達することを特徴とする多層化学分析材料集積板
の使用方法。[Scope of Claims] 1. Three or more multilayer chemical analysis materials of different types each having a reagent layer and a porous development layer are provided on a support plate, and the reagent layers are in contact with each other. or are arranged close to each other with a gap of 0.5 mm to 5 mm, and the developing layer has the same shape as the reagent layer and is provided for each reagent layer, or the developing layer has a shape larger than the developing circle and is provided for each reagent layer. A multilayer chemical analysis material accumulation board characterized by having one piece common to all. 2 A plurality of multilayer chemical analysis materials each having a reagent layer and a porous spreading layer and of different types are provided on a support plate, and the reagent layers are in contact with each other or with a gap of 0.5 mm to 5 mm. and the developing layer has the same shape as the reagent layer and is provided for each reagent layer, or the developing layer has a shape larger than a developing circle and is provided in common to all the reagent layers. A multilayer chemical analysis characterized in that a liquid sample is attached to the spreading layer of a multilayer chemical analysis material accumulation board, whereby the liquid sample is spread by the spreading layer and reaches each of the plurality of reagent layers. How to use the material accumulation board.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10685078A JPS5533651A (en) | 1978-08-31 | 1978-08-31 | Laminated plate of multi-layered chemical analysis material and using method thereof |
| DE19792934760 DE2934760A1 (en) | 1978-08-31 | 1979-08-28 | INTEGRATED MATERIAL FOR CHEMICAL ANALYSIS AND METHOD FOR APPLYING IT |
| US06/071,618 US4270920A (en) | 1978-08-31 | 1979-08-31 | Integrated material for chemical analysis and a method of using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10685078A JPS5533651A (en) | 1978-08-31 | 1978-08-31 | Laminated plate of multi-layered chemical analysis material and using method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5533651A JPS5533651A (en) | 1980-03-08 |
| JPS6151737B2 true JPS6151737B2 (en) | 1986-11-10 |
Family
ID=14444092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10685078A Granted JPS5533651A (en) | 1978-08-31 | 1978-08-31 | Laminated plate of multi-layered chemical analysis material and using method thereof |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US4270920A (en) |
| JP (1) | JPS5533651A (en) |
| DE (1) | DE2934760A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015161656A (en) * | 2014-02-28 | 2015-09-07 | 公立大学法人奈良県立医科大学 | Inspection tool of test solution physical property value |
| WO2021220730A1 (en) * | 2020-04-30 | 2021-11-04 | ウシオ電機株式会社 | Component measurement method and component measurement strip |
| WO2023162095A1 (en) * | 2022-02-24 | 2023-08-31 | セルスペクト株式会社 | Blood testing device |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5670460A (en) * | 1979-11-13 | 1981-06-12 | Fuji Photo Film Co Ltd | Multilayer chemical analysis material for analysis of water liquid |
| US4323536A (en) * | 1980-02-06 | 1982-04-06 | Eastman Kodak Company | Multi-analyte test device |
| DE3029579C2 (en) * | 1980-08-05 | 1985-12-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method and means for separating plasma or serum from whole blood |
| JPH0122135Y2 (en) * | 1980-08-25 | 1989-06-29 | ||
| SE427389B (en) * | 1981-03-02 | 1983-03-28 | Alfa Laval Ab | INDICATOR INCLUDING A CAREER AND A REACTION SYSTEM |
| JPS5977356A (en) * | 1982-06-30 | 1984-05-02 | Fuji Photo Film Co Ltd | Multilayer analysis element for fluorescent assay and fluorescent assay using the same |
| USRE34405E (en) * | 1983-08-01 | 1993-10-12 | Abbott Laboratories | Determination of analytes in particle-containing medium |
| JPH0736012B2 (en) * | 1984-10-05 | 1995-04-19 | テルモ株式会社 | Test pieces |
| US4857271A (en) | 1985-02-07 | 1989-08-15 | Eastman Kodak Company | Reducible compounds and analytical compositions, elements and methods utilizing same |
| US4678757A (en) * | 1985-04-11 | 1987-07-07 | Smithkline Diagnostics, Inc. | Device and method for whole blood separation and analysis |
| JPS61262661A (en) * | 1985-05-16 | 1986-11-20 | Konishiroku Photo Ind Co Ltd | Biochemical analysis unit |
| US4623461A (en) * | 1985-05-31 | 1986-11-18 | Murex Corporation | Transverse flow diagnostic device |
| US4857453A (en) * | 1987-04-07 | 1989-08-15 | Syntex (U.S.A.) Inc. | Immunoassay device |
| US5256372A (en) * | 1987-11-06 | 1993-10-26 | Idexx Corporation | Dipstick test device including a removable filter assembly |
| ES2056877T3 (en) * | 1987-12-23 | 1994-10-16 | Abbott Lab | DEVICE TO CONDUCT AGGLUTINATION REACTIONS. |
| US4963325A (en) * | 1988-05-06 | 1990-10-16 | Hygeia Sciences, Inc. | Swab expressor immunoassay device |
| JPH0715427U (en) * | 1990-12-29 | 1995-03-14 | デルタ工業株式会社 | Car seat |
| DE4217733A1 (en) * | 1992-05-29 | 1993-12-02 | Boehringer Mannheim Gmbh | Test carrier for analyte determination and method for its production |
| US5707818A (en) * | 1994-12-13 | 1998-01-13 | Bsi Corporation | Device and method for simultaneously performing multiple competitive immunoassays |
| US5981298A (en) * | 1995-12-13 | 1999-11-09 | Surmodics, Inc. | Immunoassay device and method |
| WO2000014535A1 (en) * | 1998-09-09 | 2000-03-16 | Amira Medical | Interstitial fluid methods and devices for determination of an analyte in the body |
| US6251083B1 (en) | 1999-09-07 | 2001-06-26 | Amira Medical | Interstitial fluid methods and devices for determination of an analyte in the body |
| US7816090B2 (en) * | 2007-01-10 | 2010-10-19 | Ortho-Clinical Diagnostics, Inc. | Multiple immunochemistry assays on an element |
| US9903858B2 (en) | 2014-07-23 | 2018-02-27 | Ortho-Clinical Diagnostics, Inc. | Multiplexing with single sample metering event to increase throughput |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3006735A (en) * | 1959-09-22 | 1961-10-31 | Morton Salt Co | Quick-dip indicator |
| US3139328A (en) * | 1960-07-21 | 1964-06-30 | Sun Oil Co | Indicator paper |
| DE2332760C3 (en) * | 1972-06-30 | 1982-03-04 | Eastman Kodak Co., 14650 Rochester, N.Y. | Material for the quantitative spectrophotometric analysis of a liquid |
| US4046512A (en) * | 1976-02-20 | 1977-09-06 | Westinghouse Electric Corporation | Device for indicating overheating in generators |
| US4050898A (en) * | 1976-04-26 | 1977-09-27 | Eastman Kodak Company | Integral analytical element |
-
1978
- 1978-08-31 JP JP10685078A patent/JPS5533651A/en active Granted
-
1979
- 1979-08-28 DE DE19792934760 patent/DE2934760A1/en active Granted
- 1979-08-31 US US06/071,618 patent/US4270920A/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015161656A (en) * | 2014-02-28 | 2015-09-07 | 公立大学法人奈良県立医科大学 | Inspection tool of test solution physical property value |
| WO2021220730A1 (en) * | 2020-04-30 | 2021-11-04 | ウシオ電機株式会社 | Component measurement method and component measurement strip |
| JP2021177169A (en) * | 2020-04-30 | 2021-11-11 | ウシオ電機株式会社 | Component measurement method and strip for component measurement |
| WO2023162095A1 (en) * | 2022-02-24 | 2023-08-31 | セルスペクト株式会社 | Blood testing device |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2934760C2 (en) | 1988-05-05 |
| JPS5533651A (en) | 1980-03-08 |
| DE2934760A1 (en) | 1980-03-13 |
| US4270920A (en) | 1981-06-02 |
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