JP3510597B2 - Blood separation device and blood test method - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a blood separation device, and more particularly to a blood separation device capable of quantitatively separating non-blood cell components from blood.

[0002]

2. Description of the Related Art Conventionally, when separating a non-blood cell component from blood, the collected blood has been centrifuged to separate the blood cell component and the non-blood cell component. That is,
Blood collected from a vein is centrifuged, and a liquid non-blood cell component, mainly a plasma component, is used as a sample for measurement by a measuring device.

Here, the non-blood cell component mainly means a plasma component or a serum component, and means something other than the blood cell component in blood. The blood cell component includes white blood cells, red blood cells, platelets and the like.

Further, as a method of conducting a medical examination by mail, conventionally, finger-tip blood, that is, blood collected from a finger, is dropped on a blood sampling filter paper, dried for a predetermined time, put in a bag and sent to an inspection agency. There is a thing to do an inspection.

[0005]

However, in the above method of centrifugation, it is necessary to perform the separation with a centrifugal separator,
A centrifuge is needed. In addition, the labor required for the centrifuge is required. In particular, when a medical examination is performed by mail, a centrifuge is required on the side of the examinee (subject), and even if the centrifuge can be performed on the side of the examinee, the sample is liquid. So
There is a problem that the transfer becomes difficult and that the test component of the sample is inactivated or denatured if it remains liquid.

Further, in the above method of dropping blood on the blood collecting filter paper, since blood adheres to the blood collecting filter paper, GO
Colorimetric measurement becomes difficult when measuring biochemical items such as T, GPT, and cholesterol. That is, since the extract obtained from the blood sampling filter paper has a red color due to hemoglobin, there is a problem that colorimetric measurement, that is, measurement by a colorimetric quantitative method cannot be performed. In addition, unlike biochemical items,
Regarding immunological items, even an extract from a blood-collecting filter paper onto which blood has been dropped, that is, an extract exhibiting a red color can be measured in that sense because only the substance to be measured can react. .

Therefore, the present invention can easily separate non-blood cell components in blood without using a centrifuge, can be used for medical examination by mail, and can be used for biochemical items. It is an object of the present invention to provide a blood separation device that does not interfere with measurement.

[0008]

The present invention has been made to solve the above problems, and firstly, a non-blood cell component, which is a component other than blood cell components, in blood. , A blood separation device for separating non-blood cell components that are plasma components or serum components (may be a "blood processing device")
And a separation part for separating non-blood cell components and a first holding part for holding the separation part, the first part having a plate shape
A separation unit having a holding unit; an adsorption unit for adsorbing the non-blood cell component separated by the separation unit; and a second holding unit having a second holding unit holding the adsorption unit and having a plate shape. A suction unit, a main body that holds the separation unit and the suction unit in a state where the separation unit and the suction unit are stacked, and an upper main body that detachably holds the separation unit. An upper main body that holds the separation unit on the lower surface side of the upper main body so that the separation section is located below the through hole, and a suction hole for dropping blood; A switching member provided between a main body portion having a lower main body portion for holding the unit, and the upper main body portion and the separation unit, wherein the through hole of the upper main body portion is closed from a closed state of closing the lower end of the through hole. Open state with open bottom And having a switching member for switching, a.

In the blood separating device of the first construction, blood is dripped into the through hole of the upper main body. At this stage, the switching member is placed in a closed state in which the lower end of the through hole is closed so that blood does not drop into the separation section. Next, the switching member brings the lower end of the through hole into an open state. Then, the separation unit is held by the upper main body part in the main body part, and the separation is positioned below the through hole, so that the blood reaches the separation part and the blood cell component is captured in the separation part, and only the non-blood cell component is obtained. Separates and moves to the lower surface side. Then, since the separation unit and the adsorption unit are held in the main body in a laminated state, the separated non-blood cell component is adsorbed to the adsorption unit. The adsorbed portion is extracted by shaking in a liquid such as physiological saline to extract non-blood cell components and used for various tests.

Therefore, the centrifugation operation is not required when separating the non-blood cell components, and the non-blood cell components can be separated by a simple method. Further, if the adsorbing part on which the non-blood cell components are adsorbed is dried, it can be stored at room temperature or transferred by mail or the like. In addition, since the red blood cells are captured by the separation unit, the adsorption unit that has adsorbed the non-blood cell component does not include hemoglobin in the red blood cell component, and thus does not affect the colorimetric quantification, and thus does not affect the immunological measurement. In addition, biochemical measurement is also possible. Further, in particular, since the lower end of the through hole can be closed by the switching member when the blood is dropped into the through hole, the blood is stored in the through hole and then the switching member is opened to the separating portion. Since the blood is dropped, a fixed amount of blood can be dropped on the separation part.

The first configuration may be the following configurations. That is, a blood separation device for separating a non-blood cell component, which is a component other than blood cell components, from blood, that is a plasma component or a serum component,
A separation unit having a separation part for separating non-blood cell components, and a first plate-shaped main body part that holds the separation part and has a plate shape, and a separation unit An adsorption unit having an adsorption unit for adsorbing the separated non-blood cell component, and a second plate-shaped main body unit having a second plate-shaped main body unit holding the adsorption unit, and the separation unit and the adsorption unit. A main body for holding the unit in a state where the separation unit and the adsorption unit are stacked, and an upper main body for detachably holding the separation unit, which has a through hole for dropping blood. An upper main body portion that holds the separation unit on the lower surface side of the upper main body portion, and a lower main body portion that holds the suction unit so that the separation portion is located below the through hole. Between the main body part and the upper main body part and the separation unit And a switching member that switches from a closed state in which the lower end of the through hole in the upper main body portion is closed to an open state in which the lower end of the through hole is opened. . In addition, it is conceivable that the switching member is omitted from the first structure.

Secondly, in the first structure, the separation part is a sheet-shaped separation membrane having a plurality of pores therein, and the diameter of the pores gradually changes in the thickness direction. It is characterized in that it is formed of the separation film. As a result, the blood cell component of blood is captured, and the non-blood cell component can be separated. When the separation unit having this separation membrane is held in the main body, the side with the larger pore diameter is held in the upper main body.

Thirdly, in the first or second construction, the suction portion is a sheet-shaped filter paper.

Fourthly, in any one of the first to third configurations, in the main body portion, the upper main body portion and the lower main body portion are connected to each other, and the upper main body portion and the lower main body portion are connected. One is rotatable with respect to the other, and the upper body and the lower body have detaching means for engaging with each other. Therefore, by locking the upper body portion and the lower body portion with the attaching / detaching means, it is possible to fix the state in which the separation unit and the suction unit are held by the body portion.

Fifthly, in any one of the above first to fourth configurations, the through hole in the upper main body portion has an inverted truncated cone shape, and the through hole in the upper main body portion is disposed on the side where the separation unit is disposed. The diameter of the through hole on the lower surface side is smaller than the diameter of the through hole on the upper surface side opposite to the lower surface side. Therefore, since the opening on the side where the blood is dropped can be widened, the blood can be dropped easily.

Sixthly, in any one of the first to fifth configurations, the penetrating is provided on an upper surface side opposite to a lower surface side on which the separation unit is disposed in the upper main body section. It is characterized in that a protrusion is formed in a region where the hole is formed so as to project more than other regions, and the through hole is formed in the protrusion. By providing this protruding portion, it becomes possible to easily drop blood.

Seventhly, in any one of the first to sixth configurations, the outer periphery of the through hole on the lower surface side of the upper main body portion on which the separation unit is disposed,
It is characterized in that the groove portion is provided in an annular shape. With this groove, it is possible to form an outflow place of the air when the blood drops, and it is possible to smoothly drop the blood to the separation part.

Eighthly, in the seventh structure, a communication hole for communicating the groove and the outer surface on the upper surface side of the upper main body is provided. In the eighth structure, the communication hole is provided,
Since one of the communication holes is opened on the outer surface of the main body, the air pushed out when the blood falls can be discharged to the outside of the main body, and the blood can be smoothly dropped to the separation section. It becomes possible to do it. In particular, when the protrusion is provided as in the sixth configuration, it is preferable to open the communication hole at the upper end of the protrusion.

Ninthly, in the seventh or eighth construction, the separating portion has a circular sheet shape, and the diameter of the separating portion is formed larger than the diameter of the groove portion. Characterize. This makes it possible to make the size of the separating portion sufficiently large.

Tenthly, in any one of the first to ninth configurations, between the lower surface of the upper main body on which the separation unit is disposed and the separation unit of the separation unit. Is characterized in that a gap is formed therein. This makes it possible to form a space into which air can escape, and in particular, when the groove portion or the communication hole is formed, the air can be efficiently discharged.

Eleventhly, in any one of the first to tenth configurations, the lower main body portion is located below the suction portion in a state where the suction unit is held by the lower main body portion. , Through holes are formed. Thereby, since the air pushed out by the blood dropping can be discharged also from the lower main body side, it is possible to promote the separation of the non-blood cell component and the adsorption of the non-blood cell component to the adsorption portion, and further, It is possible to accelerate the drying of the non-blood cell component adsorbed on the adsorption unit.

Twelfthly, in any one of the first to eleventh configurations, the first holding portion has a plate shape, and the second holding portion has a plate shape. And

Thirteenth, in any one of the first to twelfth configurations, the first holding portion in the separation unit has an opening, and the separation portion is provided at the position of the opening. In the separation unit, the lower surface on the adsorption unit side of the separation unit is formed inside the lower surface of the first holding unit, and the recess is formed in the opening of the lower surface of the separation unit. Is characterized by. With this concave portion, it is possible to form a space in which the suction portion is fitted.

In a fourteenth aspect, in any one of the first to thirteenth configurations, in the suction unit, an opening is provided in the second holding section, and the suction section is the second holding section. It is characterized in that it is provided above so as to close the opening. Therefore, the suction portion is in a state of protruding from the upper surface of the second holding portion, and the suction portion can be fitted into the recess formed in the separation unit.

Fifteenthly, in any one of the first to twelfth configurations, the first holding portion in the separation unit has an opening, and the separation portion is located at the position of the opening. In the separation unit, the lower surface of the separation unit on the suction unit side is formed inside the lower surface of the first holding unit, and the recess is formed in the opening of the lower surface of the separation unit. In the suction unit, an opening is provided in the second holding unit, the suction unit is provided on the second holding unit so as to close the opening, and the separation unit and the suction unit are stacked. In this case, the suction part is fitted in the recess formed in the separation unit. Therefore, the suction portion is in a state of protruding from the upper surface of the second holding portion, and this suction portion can be fitted into the recess formed in the separation unit. Therefore, the suction portion is located below the separation portion. It is possible to precisely and closely arrange the predetermined position.

A sixteenth feature is that, in any one of the first to fifteenth configurations, a fixing means for fixing the suction unit to the lower main body portion of the main body portion is provided. To do. Therefore, when the upper body portion and the lower body portion are released, the separation portion and the suction portion can be reliably separated.

In the seventeenth aspect, in any one of the first to sixteenth configurations, the switching member has a sheet shape, and a part of the switching member is disposed so as to be exposed from the main body portion. By moving (for example, pulling out) the switching member, the lower end of the through hole in the upper main body can be opened.

An eighteenth aspect is characterized in that, in the seventeenth configuration, the switching member is provided with an opening for allowing the blood stored in the through hole to pass therethrough. The size of the diameter of this opening is preferably equal to or smaller than the size of the diameter of the lower surface side of the through hole.

In the nineteenth aspect, in any one of the first to eighteenth configurations, when the separation unit and the adsorption unit are stacked and held by the main body portion, the separation unit is separated. The part and the suction part of the suction unit are in close contact with each other. Therefore, since the separation section and the adsorption section are in close contact with each other, it is possible to contribute to the quantification of the non-blood cell component adsorbed by the adsorption section.

Twentieth, a blood separator for separating non-blood cell components, which are components other than blood cell components, which are plasma components or serum components, from blood (“blood treatment”). Device)), which has a separation unit for separating non-blood cell components, an adsorption unit for adsorbing the non-blood cell components separated by the separation unit, and a through hole for dropping blood. A switching member provided between the main body portion and the separation portion, the main body portion being disposed so that the separation portion is located below the through hole, and the lower end of the through hole in the main body portion. A switching member that switches from a closed state to close the open state in which the lower end of the through hole is opened.

In the twentieth structure, blood is dropped into the through hole of the main body. At this stage, the switching member is placed in a closed state in which the lower end of the through hole is closed so that blood does not drop into the separation section. Next, the switching member is brought into an open state in which the lower end of the through hole is opened. Then, since the separation part is located below the through hole, the blood reaches the separation part, the blood cell component is captured in the separation part, and only the non-blood cell component is separated and moves to the lower surface side. Then, the separated non-blood cell component is adsorbed to the adsorption unit. The adsorbed portion is extracted by shaking in a liquid such as physiological saline to extract non-blood cell components and used for various tests.

Therefore, the centrifugation operation becomes unnecessary when separating the non-blood cell component, and the non-blood cell component can be separated by a simple method. Further, if the adsorbing part on which the non-blood cell components are adsorbed is dried, it can be stored at room temperature or transferred by mail or the like. In addition, since the red blood cells are captured by the separation unit, the adsorption unit that has adsorbed the non-blood cell component does not include hemoglobin in the red blood cell component, and thus does not affect the colorimetric quantification, and thus does not affect the immunological measurement. In addition, biochemical measurement is also possible. Further, in particular, since the lower end of the through hole can be closed by the switching member when the blood is dropped into the through hole, the blood is stored in the through hole and then the switching member is opened to the separating portion. Since the blood is dropped, a fixed amount of blood can be dropped on the separation part.

In the twentieth configuration, the switching member has a sheet shape, and a part of the switching member is disposed so as to be exposed from the main body portion, and the switching member is moved (for example, It is preferable that the lower end of the through hole in the upper main body can be opened so that the lower end is opened. Further, it is preferable that the switching member is provided with an opening for allowing the blood stored in the through hole to pass therethrough. In addition, the 20th
In the above configuration, the switching member may be omitted.

In a twenty-first aspect, in the twentieth configuration, the separation part is a sheet-shaped separation membrane having a plurality of pores inside, and the diameter of the pores gradually changes in the thickness direction. It is characterized in that it is formed of the separation film. As a result, the blood cell component of blood is captured, and the non-blood cell component can be separated. When the separation unit having this separation membrane is held by the main body, the side with the larger pore diameter is held as the upper side . Well
Twenty-second, in the twentieth or twenty-first configuration,
The switching member has a sheet shape,
A part of is disposed so as to be exposed from the main body,
By moving the switching member,
The lower end of the through hole can be opened.
And are characterized.

In the twentieth or twenty-first configuration, the through hole in the main body may have an inverted truncated cone shape. Further, on the upper surface side of the main body portion, a projecting portion formed so as to project more than other regions is formed in a region where the through hole is formed, and the through hole is formed in the projecting portion. You may be allowed to.
Further, the groove may be annularly provided on the outer periphery of the through hole in the main body. A communication hole may be provided to connect the groove and the outer surface on the upper surface side of the main body. Further, the separation portion may have a circular sheet shape, and the diameter of the separation portion may be formed larger than the diameter of the groove portion.

Twenty- third, in any one of the first to twenty- second configurations, by dropping a predetermined amount or more of blood into the separation part, a substantially constant amount of non-blood cell components is adsorbed in the adsorption part. It is characterized by being adsorbed on. Therefore, it is possible to ensure the quantification of the non-blood cell component adsorbed on the adsorption unit.

Twenty- fourth, in any one of the first to twenty- third constructions, the separation part has an amount exceeding the water retention capacity of the adsorption part with respect to the dropping of a predetermined amount or more of blood. It is characterized by having the ability to separate the non-blood cell components of. Therefore, it is possible to ensure the quantification of the non-blood cell component adsorbed on the adsorption unit.

In addition to the above configuration, the following configuration may be adopted. That is, a blood separation device for separating a non-blood cell component that is a component other than a blood cell component from blood, that is, a blood plasma component or a serum component, and a separation for separating the non-blood cell component. A separation part formed of a sheet-shaped separation membrane having a plurality of pores inside, the separation membrane having a structure in which the diameter of the pores gradually changes in the thickness direction, and a separation part holding the separation part. 1 plate-shaped main body,
A separation unit having a plate-like shape having an opening, and a first plate-shaped main body having a separation part fixed and held in the opening, and an adsorption part for adsorbing the non-blood cell component separated by the separation part And a second plate-shaped main body that holds the suction portion, has a plate-like shape having an opening, and is fixedly arranged on the second plate-shaped main body so as to close the opening. A main body for holding an adsorption unit having a plate-shaped main body, the separation unit and the adsorption unit in a state where the separation unit and the adsorption unit are stacked, and detachably holding the separation unit. In the upper main body portion, which has a through hole having an inverted truncated cone shape for dripping blood, on the upper surface side that is the opposite side to the lower surface side that is the separation unit installation side in the upper main body portion, In the area where the through-hole is formed, Out protruding portions formed is formed, the projecting shape portion, the through hole is formed, also,
The separation unit is supported on the lower surface side of the upper main body portion so that the separation portion is located below the through hole, and the through hole on the lower surface side of the upper main body portion on which the separation unit is arranged is formed. A groove portion is provided on the outer periphery in a circular shape, and further, an upper main body portion provided with a communication hole that communicates the groove portion with the outer surface of the upper main body portion on the upper surface side, and the adsorption unit. A lower main body portion for holding, wherein the lower main body portion has a through hole formed below the suction portion in a state where the suction unit is held by the lower main body portion. The lower main body is connected to the lower main body, the upper main body is rotatable with respect to the lower main body, and the upper main body and the lower main body are provided with attachment / detachment means for engaging with each other. Separate the main unit from the upper main unit. A sheet-like member provided between the upper and lower parts of the upper main body portion, the sheet-like member changing from a closed state in which the lower end of the through hole is closed to an open state in which the lower end of the through hole is opened. It may be a blood separating device.

The twenty-fifth is a blood test method for testing blood, which comprises a step of sending the blood separating apparatus having any one of the first to twenty- fourth structures to a examinee, and the blood separating method. At least the adsorption unit in the device, by using the blood separation device, the adsorption unit in which the non-blood cell component that is a component other than the blood cell component and the non-blood cell component that is the plasma component or the serum component is adsorbed The present invention is characterized by including a receiving step of receiving and an inspection step of performing an inspection using an adsorption part on which a non-blood cell component is adsorbed.

In a twenty- sixth aspect, in the twenty- fifth configuration, the inspection step includes an extraction liquid producing step of extracting the adsorption liquid by shaking the adsorption portion on which the non-blood cell component is adsorbed,
And a measurement step of performing a predetermined measurement using the obtained extract.

Therefore, since the predetermined measurement is carried out by using the extract obtained by shaking and extracting the adsorbing portion in which only the non-blood cell components are adsorbed, accurate measurement can be performed even in the colorimetric method. It will be possible.

[0042]

[0043]

[0044]

[0045]

[0046]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described with reference to the drawings. The blood separating apparatus (may be referred to as “blood processing apparatus”) P according to the present invention is for separating non-blood cell components in blood from blood, that is, components other than blood cell components in blood. Is for separating plasma components, serum components and the like. As shown in FIGS. 1 to 4, the blood separation device P includes a main body portion A, a separation unit B, an adsorption unit C, and a sheet-shaped member D.

First, the main body portion A will be described with reference to FIGS. The main body A has a case shape as a whole, and has a space for accommodating the separation unit B and the suction unit C therein. This body A is
It is formed of a synthetic resin material. Specifically, it is made of PP resin such as polypropylene.

The body A has an upper body 10 and a lower body 40. First, the upper body 10
The plate-shaped portion 12, the frame-shaped portion 14, the protruding portion 16, and the through hole 1
8, groove portion 20, protrusion portion 24, and locking portion 26.

Here, the plate-shaped portion 12 has a substantially rectangular plate shape. The upper surface 12a and the lower surface 12b of the plate-shaped portion 12 are parallel to each other. In addition, the frame-shaped portion 1
The reference numeral 4 is a portion projecting from the outer periphery of the plate-shaped portion 12 to the lower surface side, that is, the lower body portion 40 side. That is, the frame-shaped portions 14 are those protruding from the outer peripheral regions along the four sides of the lower surface 12b of the plate-shaped portion 12, and each of the frame portion 14a, the frame portion 14b, the frame portion 14c, and the frame portion 14d. have. In addition, a cutout portion 14-1 is provided in a part of the frame-shaped portion 14.
Is provided. That is, an elongated cutout portion 14-1 is provided near the center of the frame portion 14c adjacent to the lower body portion 40 via the connecting portion 60. This notch 1
4-1 is for arranging a sheet-shaped member D described later.

Further, a pair of claw portions 15 are formed so as to project inside the frame portion 14a, and a pair of claw portions 15 are also formed so as to project inside the frame portion 14c. The claw portion 15 is for fixing the separation unit B, and the separation unit B is sandwiched between the plate-shaped portion 12 and
The separation unit B is fixed.

Further, the protrusion 16 is formed in the upper main body 10
Is provided on the upper surface, that is, the upper surface 12a of the plate-shaped portion 12, that is, the surface on the front side, and has a substantially ring shape. The outer periphery of the projecting portion 16 in plan view is circular, and the outer side surface is inclined. A through hole 18 is provided at the center of the protrusion 16. This through hole 1
Reference numeral 8 denotes the upper surface of the upper main body portion 10, specifically, the protruding portion 16
From the upper part to the lower surface (that is, the back surface) of the upper main body portion 10, the side surface of the through hole 18 is formed in a tapered shape with an inclination, and the side surface of the through hole 18 is a plate-shaped portion. 1
It is symmetrical about an axis in a direction (Z direction) perpendicular to the plane formed by the upper surface 12a of the second. That is, the through hole 18 is
It has an inverted truncated cone shape. As a result, both the upper surface side and the lower surface side of the through hole 18 are circularly opened. That is, the opening 18-1 on the upper surface 12a side of the through hole 18 and the opening 18-2 on the lower surface 12b side of the through hole 18 are circular. In addition, the opening 18-1 is the opening 18
Greater than -2. That is, the upper surface 1 of the through hole 18
When the diameter of the opening 18-1 on the 2a side is a and the diameter of the opening 18-2 on the lower surface 12b side of the through hole 18 is b, a> b. That is, the upper body 10
The diameter of the through hole 18 on the lower surface side on which the separation unit is disposed is smaller than the diameter of the through hole 18 on the upper surface side opposite to the lower surface side. Also, this protrusion 16
The upper surface of is formed in a substantially flat shape and has a ring shape.

That is, in the region where the through hole 18 is formed, the protruding portion 16 formed so as to protrude from other regions, that is, other regions on the upper surface of the upper main body portion 10, is formed. The through hole 18 is formed in the protrusion 16.

The groove portion 20 is provided on the lower surface of the upper main body portion 10, specifically, the lower surface of the plate portion 12, that is, the back surface, and is annular (ring) around the through hole 18. Shape), that is, formed in a circumferential shape. This groove 2
The vertical cross-sectional shape of 0 is a square shape. This groove 20
A plurality of (specifically, four) through-holes (communication holes) 2 are provided between the upper end, that is, between the ceiling and the upper surface of the protrusion 16.
Two are provided. That is, the insertion hole 22 penetrates between the projecting portion 16 and the groove portion 20, is provided along the Z direction, and communicates with the outer surface of the upper main body portion 10 on the upper surface side.

The projecting portion 24 is formed by the upper body portion 10.
A pair is provided on the lower surface of, specifically, the lower surface of the plate-shaped portion 12. That is, they are arranged on both sides in the X direction with the through hole 18 and the groove portion 20 interposed therebetween. This protrusion 24 is
It has a substantially cylindrical shape, and a cylindrical hole portion 25 is provided therein. A projection 46, which will be described later, is fitted into the hole 25.

The engaging portion 26 is a detachable member for detachably attaching the upper body portion 10 and the lower body portion 40 in the closed state, and is provided on the frame-shaped portion 14 and the other engaging portion. Together with the stopper 50, the upper body 10 and the lower body 40 can be fixed in a closed state.

Further, a protruding piece 28 is formed at the corner of the back surface of the plate-shaped portion 12, and if the separating unit B is not placed in the correct direction, this protruding piece 28 will separate the separating unit B from the upper main body. The separation unit B can be set in the correct arrangement direction by being an obstacle when the separation unit B is arranged. In addition, 4 of the plate-like portion 12 of the upper main body portion 10
An elongated rectangular opening 30 is provided near one corner (see FIG. 7).

Next, the lower body portion 40 has a plate portion 42,
It has a mesh projection 44, a protrusion 46, a through hole 48, and a locking portion 50.

Here, the plate-shaped portion 42 has a substantially rectangular plate shape, and the upper surface 42a and the lower surface 42 of the plate-shaped portion 42.
b are parallel to each other. The size / shape of the plate-shaped portion 42 in plan view is smaller than that of the plate-shaped portion 12 in the upper body 10.

Further, the mesh projection 44 is provided on the upper surface 42a of the plate 42, and is formed so as to project in a substantially mesh shape as shown in FIG. The mesh protrusions 44 are formed to maintain the rigidity of the lower body 40. The shape of the mesh protrusion 44 is formed parallel to the long side and the short side of the plate-like portion 42, but its central portion is doubled as shown in FIGS. 5 and 6. It is formed in a circular shape.
That is, the circular protrusion 49-1 and the circular protrusion 49-1
A circular protrusion 49-2 having a diameter larger than that of the circular protrusion 49-2 is formed, so that the circular protrusion 49-2 protrudes in a double circle.

A pair of protrusions 46 are provided on the upper surface of the plate portion 42. That is, they are arranged on both sides in the X direction with the through hole 48 interposed therebetween. This protrusion 46
Has a columnar shape, and is provided at a position corresponding to the position of the hole 25 when the upper body 10 is closed with respect to the lower body 40. That is, when the upper main body portion 10 is closed with respect to the lower main body portion 40, the projecting portion 46 is fitted into the hole portion 25 and enters.

Further, the through hole 48 is provided substantially in the center of the lower body portion 40. That is, this through hole 48 is
Inside the circular protrusion 49-1, the circular protrusion 49-
It is provided at the center position of the area surrounded by 1. The through hole 48 is formed from the upper surface 42 a of the lower body 40 to the lower surface 42 a.
It is a through hole that penetrates to b and has a cylindrical shape. The through hole 48 is provided at a position corresponding to the position of the through hole 18 when the upper body 10 is closed with respect to the lower body 40. That is, when the upper body 10 is closed with respect to the lower body 40, the circle formed by the through hole 18 and the circle formed by the through hole 48 are concentric circles in a plan view. . That is, the through hole 18
The vertical axis (Z direction) of the through hole 48 and the vertical axis (Z direction) of the through hole 48 coincide with each other. When the suction unit C is held by the lower body 40, the through hole 48 is located below the suction filter paper 130 of the suction unit C. At that time, the adsorption filter paper 1
The center of 30, that is, the axial center and the axial center of the through hole 48 coincide with or substantially coincide with each other. The diameter of the through hole 48 is smaller than the diameter of the opening 18-2 on the lower surface side of the upper body portion 10 of the through hole 18. That is, when the diameter of the through hole 48 is c, b> c (FIGS. 8 and 1).
2). 8 is a sectional view of the main body A,
The cutting position is the same as the XX position in FIG. 12 is also a cross-sectional view of the main part of the blood separation device P, the cutting position of which is the same as the XX position in FIG.

The locking portion 50 is a detachable member for detachably fixing the upper body portion 10 and the lower body portion 40 in the closed state, and is provided at the front end of the plate portion 42.
Together with the other locking portion 26, the upper body 10 and the lower body 40 can be fixed in a closed state. That is, when the upper body portion 10 and the lower body portion 40 are closed, the locking portion 26 and the locking portion 50 are locked and coupled with each other. In addition, the locking portion 26 in the coupled state
The locking portion 26 and the locking portion 50 are operated by a force greater than a predetermined value so as to separate the locking portion 50 from the locking portion 50 in the vertical direction.
Are separated from each other so that the upper body 10 and the lower body 40 can be opened. The locking portion 26 and the locking portion 50 function as the attaching / detaching means.

A plurality of grooves 52 are formed on the lower surface 42b of the lower body 40.

The upper body 10 and the lower body 4 are also provided.
0 is connected by a connecting portion 60. That is, a pair of connecting portions 60 are provided at predetermined intervals between the end portion along the longitudinal side of the upper body portion 10 and the end portion along the longitudinal side of the lower body portion 40. The connecting portion 60 is formed so as to be easily bent, so that the upper main body portion 10 and the lower main body portion 40 can be easily opened and closed. That is, one of the upper body 10 and the lower body 40 is rotatable with respect to the other. A storage space is formed inside the main body A when the upper main body 10 and the lower main body 40 are closed. This storage space is a space for storing the separation unit B and the suction unit C.

Next, the separation unit B will be described. As shown in FIG. 9, the separation unit B has a main body portion (first holding portion, first plate-shaped main body portion) 100 and a separation membrane (separation portion) 110.

The main body 100 has a substantially plate shape, and its size and shape are formed so that it can be stored in the storage space formed in the main body A. The main body 100 has an upper plate-shaped portion 102 and a lower plate-shaped portion 104, and the upper plate-shaped portion 102 and the lower plate-shaped portion 104 are adhered by an adhesive layer 106. The adhesive layer 106 is provided over the entire surface of the region where the upper plate-shaped portion 102 and the lower plate-shaped portion 104 are in contact with each other. The upper plate-shaped portion 102 and the lower plate-shaped portion 104 are formed of a synthetic resin material, specifically, a PET resin such as polyethylene terephthalate (polyethylene terephthalate may be used; the same applies to the following).

The upper plate-shaped portion 102 has a plate-like shape in which a rectangular cutout 100K is formed at one corner of the rectangular shape, and a large-diameter circular opening 103 is formed in the center thereof.
And has small-diameter circular openings on the left and right sides of the circular opening 103. The large-diameter circular opening 103 forms a space for arranging the separation membrane 110. Further, in the main body 100, the small-diameter circular opening forms openings 108a and 108b for fitting with the protrusion 24 together with the subtotal circular opening provided in the lower plate-shaped portion 104. The notch 100K is for specifying the direction of the separation unit B.

The lower plate-shaped portion 104 also has an outer shape similar to that of the upper plate-shaped portion 102, and the center thereof is
A large-diameter circular opening 105 is provided, and small-diameter circular openings 105 are provided on the left and right of the circular opening 105. The size of the large-diameter circular opening 105 located at the center is as described above. It is slightly smaller than the circular opening 103. Further, the size and forming position of the small-diameter circular opening portion are the above-mentioned upper plate-shaped portion 102.
Similar to the above, the openings 108a and 108b are formed together with the small-diameter circular opening provided in the upper plate-shaped portion 102. The main body 100 holds the separation membrane 110.

The separation membrane 110 is in the shape of a circular sheet and is arranged inside the circular opening 103. More specifically, the adhesive layer 106 is exposed in the circular opening 103 in a ring shape, but the separation film 110 is
The adhesive layer 106 is fixed to the main body 100 by adhering to a ring-shaped exposed portion. The separation film 110 and the adhesive layer 106 are adhered to each other by the adhesive layer 112.
Done through. The adhesive layers 106 and 112 are composed of, for example, double-sided adhesive tape.

The diameter e of the separation membrane 110 is equal to
Is formed larger than the diameter d of the groove portion 20 in the upper main body portion 10. That is, as shown in FIG. 12, e> d
Has become.

The separation membrane 110 is made of polysulfone as a material and has a large number of pores inside. The thickness of the separation membrane 110 is about 300 μm, and the pore diameter of the pores is 40 to 60 μm on the front surface side, becomes smaller toward the back side, and is about 2 μm on the back surface side. In FIG. 9C, the pore diameter is large on the upper side of the separation membrane 110, and the pore diameter is small on the lower side. That is, blood cell components are captured in a so-called sparse layer near the front surface, and non-blood cell components are filtered in a so-called dense layer near the back surface. The separation membrane 110 is for separating non-blood cell components such as plasma components and serum components.

The separation membrane 110 has the ability to separate an amount of non-blood cell components that exceeds the water retention capacity of the adsorption filter paper 130 against the dropping of a predetermined amount or more of blood.

Further, in the separation film 110, as shown in FIG. 9, a recess 114 is formed on the lower surface thereof. That is, the lower surface of the separation film 110 is formed inside the lower surface of the main body 100, that is, the lower surface of the lower plate-shaped portion 104, and the recess 114 is formed in the lower surface of the separation unit B. When the separation unit B and the suction unit C are stacked, the suction filter paper 130 of the suction unit C is fitted in the recess 114. Also, the upper plate-shaped portion 1
Since the thickness of the separation membrane 110 is slightly smaller than the thickness of 02,
The recess 116 having a shallow depth is formed. As a result, even when the separation unit B is held by the upper main body 10 and the upper surface of the separation unit B is in contact with the lower surface of the upper main body 10, the lower surface of the upper main body 10 and the separation film 110 A gap is formed between them.

Next, the suction unit C will be described.
As shown in FIG. 10, the suction unit C includes a main body (second
It has a holding portion, a second plate-shaped main body portion) 120, and a suction filter paper (suction portion, suction body) 130.

The main body 120 has a substantially plate shape, and its size and shape are formed so that it can be stored in the storage space formed in the main body A. Specifically, as shown in FIG. 10, a rectangular notch 1 is formed at one corner of the rectangle.
It has a plate shape in the shape of 20K, has a large diameter circular opening 122 in the center thereof, and has small diameter circular openings 124a and 124b on the left and right of the circular opening 122. . The circular openings 124a and 124b are formed by the protrusion 4
It is formed in a size and a shape that can be fitted in 6. Also,
As shown in FIG. 10, the main body 120 has a size slightly smaller than that of the main body 100 of the separation unit B. The main body 120 is made of a synthetic resin material, specifically,
It is made of PET resin such as polyethylene terephthalate. The notch 120K is the separation unit B.
It is for specifying the direction of. The main body 120 holds the suction filter paper 130.

The suction filter paper 130 is in the shape of a circular sheet and is arranged on the upper surface of the main body 120 so as to close the circular opening 122. That is, the main body 12
0 and the adsorption filter paper 130 are adhered to each other via the adhesive layer 132. The adhesive layer 132 has a ring shape. Further, the suction filter paper 130 is arranged so that the center of the circle formed by the suction filter paper 130 and the center of the circle formed by the circular opening 122 coincide with each other.

In this embodiment, the filter paper is taken as an example of the member for adsorbing the non-blood cell component, but the member is not limited to the filter paper, and another adsorbing member may be used.

As shown in FIG. 10, an adhesive layer 134 and a release sheet 136 are provided on the back surface of the main body 120. By peeling off the release sheet 136, the adhesive layer 134 is provided on the back surface of the adsorption unit C. Can be exposed. The adhesive layer 134 corresponds to the “fixing means for fixing the suction unit and the lower main body portion of the main body portion”.

The adhesive layer 132 and the adhesive layer 134 are
For example, it is formed of a double-sided adhesive tape.

In particular, the thickness when the separation unit B and the suction unit C are overlapped is almost the same as the thickness f (see FIG. 8) of the storage space of the main body A, and Set the suction unit C in the main body A,
In the case of the state shown in FIG. 2, the separation unit B and the suction unit C are fixed in the main body portion A in the vertical direction. Since the separation unit B is fitted in the projecting portion 24 and the suction unit C is also fitted in the projecting portion 46, the separation unit B is fixed in the main body portion A even in the planar direction.

The sheet-like member D has a substantially strip-like sheet shape and is formed of a synthetic resin film, specifically, a PET resin film such as polyethylene terephthalate. As shown in FIG. 3, the sheet-shaped member D has a base end portion 200 and a front end portion 202. The width h of the base end portion 200 of the sheet-shaped member D is the frame-shaped portion 1 described above.
4 is formed to have a width equal to or smaller than the width of the cutout portion 14-1.
As a result, in the assembled blood separation device P, the sheet-shaped member D can be arranged in a state of protruding from the main body portion A, and the sheet-shaped member D can be pulled out after dropping the blood. Become. In addition, this sheet-shaped member D
The width i of the tip end portion 202 is smaller than the width h of the base end portion, and the shape of the tip end portion 202 is a substantially square shape, and the two corners thereof are rounded. There is. The width i of the tip portion 202 is formed larger than the diameter e of the separation membrane 110 (see FIG. 12 and the like). That is,
i> e. That is, the sheet-shaped member D is
When the sheet-shaped member D is arranged in the blood separation device P, it has a size sufficient to completely cover the circular opening 103 in the separation unit B. Further, as a matter of course, when the sheet-shaped member D is arranged in the blood separation device P, the sheet-shaped member D is formed so as to close the opening portion 18-2 on the lower surface side of the upper main body portion 10. The sheet-shaped member D functions as the above-mentioned "switching member for switching from the closed state in which the lower end of the through hole in the upper main body portion is closed to the open state in which the lower end of the through hole is opened".

The process of assembling the blood separator P having the above structure will be described. First, the separation unit B, the suction unit C, and the sheet-shaped member D are set in the main body portion A. That is, the sheet-shaped member D is arranged on the lower surface of the upper main body portion 10 of the main body portion A, that is, on the back surface, and further, the separation unit B is arranged on the lower surface of the upper main body portion 10. When arranging the sheet-shaped member D, the front end portion 202 of the sheet-shaped member D is placed in the opening 18 of the upper main body portion 10.
-2 and the groove portion 20 are placed at positions to be closed. More specifically, it is preferable that the center position of the leading end portion 202 of the sheet-shaped member D coincides with the center position of the opening 18-2. The base end portion 200 continuing from the front end portion 202 of the sheet-shaped member D is made to reach the outside of the main body portion A through the cutout portion 14-1 in the upper main body portion 10.

Regarding the separation unit B, the openings 108a, 108 of the separation unit B are arranged so that the upper plate-like portion 102 of the separation unit B faces the upper main body 10 side.
The protruding portion 24 protruding from the lower surface of the upper main body portion 10 is inserted into b, and is locked by the claw portion 15 so that the separation unit B is held by the upper main body portion 10 and fixed to the main body portion A. Then, the separation unit B is
It will be stored in the space on the back side of.

As for the adsorption unit C, the lower main body portion 40 is held with the side provided with the adsorption filter paper 130 as the upper side. That is, the opening 1 of the suction unit C
By inserting the protruding portion 46 protruding from the upper surface of the lower main body portion 40 into the 24a and 124b, the suction unit C
Is fixed to the main body A. At that time, the peeling sheet 136 provided on the lower surface of the suction unit C is peeled off, and then the protruding portion 46 is inserted and set on the lower main body portion 40. Therefore, when the suction unit C is set on the lower body 40, the lower surface of the suction unit C is covered by the adhesive layer 134.
It will be fixed by adhering to the upper surface of 0.

As described above, when the separation unit B, the suction unit C, and the sheet-shaped member D are set, the upper main body 10 is rotated toward the lower main body 40, and the upper main body 10 and the lower main body 10 are rotated. The main body 40 is closed. Then,
The locking portion 26 and the locking portion 50 are fixed to each other and the closed state is fixed. As described above, the assembly of the blood separation device P is completed.

When the assembly of the blood separation device P is completed, the blood separation device P becomes as shown in FIGS. 2 and 12, and the main body is shown with the separation unit B and the adsorption unit C stacked. It is in a state of being tightly housed in the space in the section A. That is, at least the central portion 10C of the lower surface (back surface) of the upper main body portion 10 (the central portion 10C is an area including the outer portion of the groove portion 20 as shown in FIG. 5).
And the sheet-shaped member D come into close contact with each other, and the sheet-shaped member D
And the upper surface of the separation unit B are in close contact with each other. That is, the upper surface of the upper plate-shaped portion 102 of the separation unit B and the lower surface of the sheet-shaped member D are in close contact with each other.

Further, at the position below the through hole 18, the separation membrane 1
10 is located. At that time, the center of the circle formed by the through-hole 18 and the center of the separation membrane 110 coincide or substantially coincide with each other.

Further, as shown in FIG. 11, the suction filter paper 130 in the suction unit C is the same as the concave portion 1 of the separation unit B.
The separation membrane 110 and the adsorptive filter paper 130 are attached to each other and are in close contact with each other. That is, the adsorption filter paper 130 is in intimate contact with the bottom of the separation membrane 110. At that time, the center of the separation membrane 100 and the center of the adsorption filter paper 130 are aligned or substantially aligned.

In FIG. 2, the sheet-like member D is drawn in a straight line in order to prevent the drawing from being difficult to see, and for the same reason, in the separation unit B,
The configurations other than the separation membrane 110 are drawn as one unit,
Further, in the suction unit C, the components other than the suction filter paper 130 are integrally drawn. Further, also in FIG. 12, the sheet-like member D is drawn by a straight line.

The state of use of the blood separator P having the above structure will be described. First, the blood separation device P of the present embodiment is sent by mail or the like from a shipping source such as an inspection institution to the examinee. At this time, the blood separation device P is installed in a storage container Q as shown in FIG.
Then, the storage container Q is placed in a bag such as a vinyl bag and sent. The storage container Q has a storage portion with an open top, and specifically, the storage portion 3 has a substantially square shape in plan view.
00, and elongated rectangular storage units 310 and 312 in plan view.
have. The storage section 300 is a storage section for storing the blood separation device P, and has a size and a shape capable of storing the main body section A in an open state. Moreover, the storage part 310 and the storage part 312 are spaces for accommodating accessories, for example, a lancet, a bandage, and alcohol cotton. The lancet is a device for collecting blood from a fingertip or the like. In other words, when sending from the shipping source to the examinee, the blood separating device P is stored in the storage part 300 in the storage container Q, and the lancet, the adhesive plaster, and the alcoholic cotton are stored in the storage part 31.
After putting it in 0, 310, store it in a bag and send it. In addition,
In FIG. 13, the mode of sending from the examinee to the inspection organization is shown. Therefore, although the upper main body part 10 and the lower main body part 40 in the main body part A are in an open state, they are sent from the sender to the examinee. At this time, the container is stored in the storage unit 300 in the state shown in FIG.

The examinee who has received the blood separation device P and the accessories mentioned above spots the blood obtained from the fingertip using a lancet or the like. At this time, the dropped blood is spotted so as to fill the through hole 18 of the upper body 10. At that time, the protrusion 1
Since 6 is provided, the periphery of the opening 18-1 on the upper surface 12a side of the through hole 18 is surrounded by the upper main body 10
Since it projects more than the planar area of, the blood can be dropped easily. Further, since the through hole 18 is tapered and the opening 18-1 on the upper surface 12a side is larger than the opening 18-2 on the lower surface 12b side, it is easy to drip blood. At this stage, since the sheet-shaped member D closes the opening 18-2 of the through hole 18 on the lower surface 12b side, blood does not move below the lower end of the through hole 18.

When the blood is dropped to fill the through hole 18, the sheet member D is removed. That is, the sheet-shaped member D is removed by pulling out the portion exposed to the outside.

Then, the blood filling the inside of the through hole 18 is dropped on the surface side of the separation membrane 110, and gradually separated.
The inside of 10 moves downward. At that time, blood cell components in the blood are captured by the surface-side layer of the separation membrane 110, and only non-blood cell components such as plasma components and serum components are separated.
To reach the adsorption filter paper 130. That is, only the non-blood cell component is adsorbed on the adsorption filter paper 130.

In this case, the lower surface 12b of the upper body 10
Since the groove portion 20 is provided around the opening 18-2 and the insertion hole 22 that communicates with the groove portion 20 is provided, when the blood drops into the separation membrane 110, the separation membrane 11
Since the air existing above 0 and in the separation membrane 110 is smoothly pushed out, it becomes possible to smoothly drop blood into the separation membrane 110 and shake it into the separation membrane 110.

The size of the separation membrane 110 is determined by the size of the through hole 1
8 is sufficiently larger than the size of the opening 18-2 on the lower side, in particular, since the diameter e of the separation membrane 110 is larger than the diameter d of the groove portion 20, the blood that has dropped onto the separation membrane 110 is separated in a plan view. It does not protrude from the membrane 110.

The adsorption filter paper 130 is the separation unit B.
Since the adsorption filter paper 130 is disposed in the recess 114 of the separation filter 110, the arrangement position of the adsorption filter paper 130 in plan view does not deviate from the separation membrane 110, and the adsorption filter paper 130 can be accurately arranged at a predetermined position below the separation membrane 110. Is possible. Therefore, the separated non-blood cell components can be reliably captured by the adsorption filter paper 130.

Further, since the lower body portion 40 is provided with the through hole 48, adsorption of the non-blood cell component is further promoted. That is, since the air existing in the traveling direction of the falling blood escapes through the through hole 48, the transfer of the non-blood cell component from the separation membrane 110 to the adsorption filter paper 130 is promoted as compared with the case where the through hole 48 is not provided. Further, even when the adsorption filter paper 130 adsorbing the non-blood cell component is dried, the through hole 4
8 further accelerates the drying. The blood separation device P
In the above, by dropping a predetermined amount or more of blood onto the separation membrane 110, a substantially constant amount of non-blood cell components is adsorbed on the adsorption filter paper.

As described above, after the adsorption of the non-blood cell component is completed, the upper main body portion 10 and the lower main body portion 40 in the main body portion A are opened after a lapse of a predetermined time. Then, since the separation unit B is fixed to the upper body portion 10 and the adsorption unit C is fixed to the lower body portion 40, the separation unit B and the adsorption unit C are separated and the separation membrane 1
10 and the suction filter paper 130 are separated.

Then, the blood separation device P is stored in the storage container Q and sent to the laboratory. That is, as shown in FIG.
With the separation unit B and the adsorption unit C opened 180 degrees, the blood separation device P is stored in the storage container Q, and the storage container Q is stored in a bag and then sent to a laboratory by mail or the like. To In the state shown in FIG.
Since the separation membrane 110 and the adsorption filter paper 130 are stored in the storage container Q in a separated state, the non-blood cell component adsorbed on the adsorption filter paper 130 is affected by the blood cell component attached to the separation membrane 110. There is no.

In the laboratory, the adsorption filter paper 130 is taken out from the blood separating apparatus P sent to it, and the adsorption filter paper 130 is shaken and extracted to obtain an extract. The adsorptive filter paper 130 corresponds to the above-mentioned “adsorbent that is a non-blood cell component other than blood cell components and has a non-blood cell component that is a plasma component or a serum component adsorbed thereto”. The step of obtaining this extract corresponds to the above-mentioned "extract producing step" in the blood test method of the present invention. That is, the adsorption filter paper 130 is put into a liquid such as physiological saline and shaken for extraction. Adsorption filter paper 13
Since only non-blood cell components are adsorbed on 0, the obtained extract is substantially transparent. This extract is used for measurement in various tests as a dilution of a normal plasma sample.
That is, this corresponds to the above “measurement step”.

When measuring biochemical items, the colorimetric method is mainly used. That is, a reagent is added to the obtained extract solution one after another to develop a color, and the measurement is performed based on the shade of the color. At that time, suction filter paper 13
Only non-blood cell components were adsorbed on 0, and red components such as hemoglobin were not mixed in the obtained extract, so color interference did not occur and accurate measurement by colorimetric method was performed. It becomes possible to do it.

Unlike the biochemical item, the immunological item can be measured using an extract obtained by shaking and extracting the adsorption filter paper 130.
Immunological items include, for example, PSA (prostate-specific antigen) used for screening for prostate cancer, measurement of pepsinogen used for screening for gastric cancer, and the like.

As described above, according to the blood separation apparatus P of this embodiment, the centrifugal separation operation is not necessary when separating the non-blood cell components, and the plasma can be quantitatively separated by a simple method. . Also, an adsorption filter paper 1 on which non-blood cell components are adsorbed
If 30 is dried, it can be stored at room temperature or transferred by mail or the like. Further, since the red blood cells are captured by the separation membrane 110, the adsorption filter paper 13 on which the non-blood cell components are adsorbed
Since 0 does not contain hemoglobin in the erythrocyte component, it does not affect the colorimetric determination, and enables biochemical measurement as well as immunological measurement.

In the above description, the sheet-shaped member D has been described as having the shape shown in FIG. 3, but the present invention is not limited to this. For example, the sheet-shaped member D may have the shape and size shown in FIG. Good. That is, the base end portion 200 has
Although it has the same shape as in the case of
In regard to No. 4, a circular opening portion 204 is provided on the tip side. Further, in FIG. 14, the width i of the tip portion 202 is
Is smaller than the width h of the base end portion 200, and the through hole 1
8 is formed to be slightly larger than the diameter of the opening 18-2. In addition, the diameter of the opening 204 is determined by the through hole 18
Is smaller than the diameter of the opening 18-2.

When setting the sheet-shaped member D, as shown in FIG.
The sheet-shaped member D is arranged so that the sheet is near the through hole 18. However, of course, the tip portion 20 of the sheet-shaped member D
2 needs to close the lower end of the through hole 18.

After the blood is dropped into the through hole 18, the sheet member D is pulled so that the opening 204 comes to the position of the through hole 18. Then, blood falls through the opening 204 and reaches the separation membrane 110. As described above, in this case, since the sheet-shaped member D is provided with the opening portion 204, when the sheet-shaped member D is set in the main body portion A, the opening portion 204 is provided.
Is arranged near the through hole 18, the sheet-shaped member D
Since a short drawing distance is required, it is possible to minimize the bleeding of blood between the upper main body portion 10 of the main body portion A and the sheet-shaped member D. That is, when the sheet-shaped member D is pulled out and the pull-out distance is long,
The amount of blood that oozes out increases accordingly, but with the configuration shown in FIG. 14, the distance for pulling out the sheet-shaped member D can be short, and therefore blood can be drawn from the upper main body 10 of the main body A and the sheet-shaped member D. It is possible to minimize the bleeding during.

In the above description, in the main body portion A, the upper main body portion 10 and the lower main body portion 40 are connected by the connecting portion 6.
Although it has been described that they are connected to each other through 0 and are rotatable with respect to each other, the present invention is not limited to this. For example, the upper main body portion 10 and the lower main body portion 40 may be separated and detachable. Good.

[0108]

According to the blood separation device of the present invention,
Centrifugation operation is not required when separating the non-blood cell components, and the non-blood cell components can be separated by a simple method. Further, if the adsorbing part on which the non-blood cell components are adsorbed is dried, it can be stored at room temperature or transferred by mail or the like. In addition, since the red blood cells are captured by the separation unit, the adsorption unit that has adsorbed the non-blood cell component does not include hemoglobin in the red blood cell component, and thus does not affect the colorimetric quantification, and thus does not affect the immunological measurement. In addition, biochemical measurement is also possible. Also,
In particular, when the blood is dropped into the through hole, the lower end of the through hole can be closed by the switching member, so that the blood is stored in the through hole, and then the switching member is opened so that the blood is separated. Since it is dropped, a fixed amount of blood can be dropped to the separation section.

[0109]

[Brief description of drawings]

FIG. 1 is a perspective view showing a configuration of a blood separation device according to an embodiment of the present invention.

FIG. 2 is a sectional view taken along line XX in FIG.

FIG. 3 is a plan view showing the configurations of a blood separation device and a sheet-shaped member according to an embodiment of the present invention.

FIG. 4 is a perspective view showing a state during assembly of the blood separation device according to the embodiment of the present invention.

FIG. 5 is a perspective view showing a main body of the blood separation device.

FIG. 6 is a plan view showing a state in which an upper body portion and a lower body portion of the blood separation device are opened 180 degrees.

FIG. 7 is a bottom view showing a state where the upper body portion and the lower body portion of the blood separation device are opened 180 degrees.

FIG. 8 is a cross-sectional view of a main body of the blood separation device.

9A and 9B are views showing a configuration of a separation unit, FIG. 9A is a plan view thereof, FIG. 9B is a bottom view thereof, and FIG.
FIG. 7A is a sectional view taken along line YY of (a).

FIG. 10 is a diagram showing a configuration of a separation unit, (a)
Is a plan view thereof, (b) is a bottom view thereof,
(C) is a ZZ sectional view of (a).

FIG. 11 is a cross-sectional view of essential parts showing a state in which a separation unit and a suction unit are stacked.

FIG. 12 is a cross-sectional view of essential parts of a blood separation device according to an embodiment of the present invention.

FIG. 13 is a perspective view showing a storage state of the blood separation device in a storage container.

FIG. 14 is a plan view showing the configurations of the blood separation device and the sheet-shaped member according to the embodiment of the present invention.

[Explanation of symbols]

P blood separation device A body B separation unit C adsorption unit D sheet-like member 10 Upper body 18, 48 through holes 20 groove 22 insertion hole 24, 46 Projection 26, 50 Locking part 40 Lower body 100, 120 Main body 102 upper plate 104 Lower plate 110 separation membrane 130 Adsorption filter paper 200 base end 202 Tip 204 opening

Continuation of front page (56) Reference JP-A-8-43294 (JP, A) JP-A-1-302161 (JP, A) JP-A-2000-74908 (JP, A) JP-A-11-295299 (JP, A) JP-A-10-132800 (JP, A) JP-A-4-212060 (JP, A) JP-A-61-66944 (JP, A) JP-A-10-104226 (JP, A) JP-A-8 -159932 (JP, A) JP-A-11-118795 (JP, A) Actual development Sho 62-143243 (JP, U) (58) Fields investigated (Int.Cl. ⁷ , DB name) G01N 33/48

Claims (26)

(57) [Claims] 1. A blood separation device for separating non-blood cell components other than blood cell components, which are non-blood cell components, which are plasma components or serum components, from blood. A separation unit for holding the separation unit, and a first holding unit for holding the separation unit, and an adsorption unit for adsorbing the non-blood cell component separated by the separation unit,
An adsorption unit having a second holding unit that holds the adsorption unit, and a main body unit that holds the separation unit and the adsorption unit in a state in which the separation unit and the adsorption unit are stacked, An upper main body portion that detachably holds the unit, has a through hole for dropping blood, and has a through-hole for dropping blood, and a lower surface side of the upper main body portion so that the separating portion is located below the through-hole. A switching member provided between the upper main body portion holding the separation unit and a lower main body portion holding the suction unit, and a switching member provided between the upper main body portion and the separation unit. And a switching member that switches from a closed state in which the lower end of the through hole is closed to an open state in which the lower end of the through hole is opened. 2. The separation portion is a sheet-shaped separation membrane having a plurality of pores inside, and is formed of a separation membrane having a structure in which the diameter of the pores gradually changes in the thickness direction. The blood separation device according to claim 1. 3. The blood separation apparatus according to claim 1, wherein the adsorption unit is a sheet-shaped filter paper. 4. In the main body, the upper main body and the lower main body are connected to each other, and one of the upper main body and the lower main body is rotatable with respect to the other. The blood separation apparatus according to claim 1, 2 or 3, wherein the main body portion has attachment / detachment means for engaging with each other. 5. The through hole in the upper main body has an inverted truncated cone shape, and the diameter of the through hole on the lower surface side of the upper main body on which the separation unit is arranged is opposite to the lower surface side. The blood separation device according to claim 1, wherein the diameter of the through hole on the upper surface side is smaller than that of the through hole. 6. On the upper surface side, which is the side opposite to the lower surface side on which the separation unit is disposed, of the upper main body portion, the through hole is formed in a region projecting from other regions. 3. A projecting portion is formed, and the through hole is formed in the projecting portion.
Or the blood separation apparatus according to 3 or 4 or 5. 7. The groove portion is annularly provided on the outer periphery of the through hole on the lower surface side of the upper main body portion on the side where the separation unit is arranged in the upper main body portion. Or the blood separation device according to item 6. 8. The blood separation apparatus according to claim 7, further comprising a communication hole that connects the groove and the outer surface of the upper main body on the upper surface side. 9. The blood separating apparatus according to claim 7, wherein the separating portion has a circular sheet shape, and the diameter of the separating portion is formed to be larger than the diameter of the groove portion. 10. A gap is formed between a surface of the upper main body portion on the lower surface side where the separation unit is disposed and a separation portion of the separation unit. Or 3 or 4 or 5 or 6 or 7 or 8
Or the blood separation device according to item 9. 11. A through hole is formed in the lower main body portion at a position below the suction portion in a state where the suction unit is held by the lower main body portion, or a through hole is formed. The blood separation apparatus according to 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10. 12. The first holding part has a plate shape, and
The said 2nd holding | maintenance part presents plate shape, 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9
Alternatively, the blood separation device according to 10 or 11. 13. The lower surface of the separation unit, wherein the first holding portion has an opening, and the separation portion is held at the position of the opening, and the separation unit has a lower surface on the adsorption unit side. Is formed inside the lower surface of the first holding portion, and a recess is formed in the opening portion of the lower surface of the separation unit.
Or the blood separation device according to 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12. 14. The suction unit according to claim 2,
An opening is provided in the holding part, and the suction part is provided on the second holding part so as to close the opening. 7. Or 7
Or the blood separation device according to 8 or 9 or 10 or 11 or 12 or 13. 15. A lower surface of the separation unit, wherein the first holding portion has an opening, and the separation portion is held at a position of the opening, and in the separation unit, the lower surface of the separation portion is on the adsorption unit side. Is formed inside the lower surface of the first holding portion, a recess is formed in the opening of the lower surface of the separation unit, and in the suction unit, the opening is provided in the second holding portion. The adsorption unit is provided on the second holding unit so as to close the opening, and when the separation unit and the adsorption unit are stacked, the adsorption unit is provided in the recess formed in the separation unit. It is fitted, The 1 or 2 or 3 or 4 or 5 or 6 characterized by the above-mentioned.
Or the blood separation device according to 7 or 8 or 9 or 10 or 11 or 12. 16. A fixing means for fixing the adsorption unit and a lower main body portion of the main body portion is provided, wherein the fixing unit is provided in one or two or three or four or five or six or seven or eight or. 9 or 10 or 11 or 12 or 1
The blood separation apparatus according to 3 or 14 or 15. 17. The switching member has a sheet shape, and a part of the switching member is disposed so as to be exposed from the main body portion. By moving the switching member, a through hole in the upper main body portion is formed. The open state in which the lower end of is open can be set to 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 or 16. The blood separation device described. 18. The blood separation apparatus according to claim 17, wherein the switching member is provided with an opening for allowing the blood stored in the through hole to pass therethrough. 19. The separation unit of the separation unit and the suction unit of the suction unit are in close contact with each other when the separation unit and the suction unit are stacked and held by the main body. Item 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 or 16 or 1
The blood separation apparatus according to 7 or 18. 20. A blood separation device for separating a non-blood cell component, which is a component other than a blood cell component, which is a plasma component or a serum component, from blood, the non-blood cell component being separated. A separating part for adsorbing the non-blood cell components separated by the separating part, and a through hole for dropping blood, so that the separating part is located below the through hole. And a switching member provided between the main body portion and the separation portion, the closed state closing the lower end of the through hole in the main body portion to the open state opening the lower end of the through hole. A blood separation device comprising: a switching member for switching. 21. The separation portion is a sheet-shaped separation membrane having a plurality of pores inside, and is formed of a separation membrane having a structure in which the diameter of the pores gradually changes in the thickness direction. The blood separation apparatus according to claim 20, wherein 22. The switching member is in the form of a sheet,
Note that a part of the switching member is not
By moving the switching member,
Make sure that the bottom of the through hole in the main unit is open.
22. The blood separation device according to claim 20 , wherein the blood separation device is capable of: 23. A predetermined amount or more of blood is dripped into the separating section.
By doing so, a substantially constant amount of non-blood cell components is absorbed in the adsorption part.
It is adsorbed, 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 or 16 or 17 or 18 or 19 or 20 or blood fraction <br/> away apparatus according to 21 or 22. 24. The separation unit has a predetermined amount of blood or more.
The amount of non-driving water that exceeds the water retention capacity of the adsorption unit
Claims characterized by having the ability to separate blood cell components
Item 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or
9 or 10 or 11 or 12 or 13 or 14 or 15
Or 16 or 17 or 18 or 19 or 20 or 21 or
22. The blood separation apparatus according to 22 or 23 . 25. A blood test method for testing blood.
Therefore, claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8
Or 9 or 10 or 11 or 12 or 13 or 14 or
15 or 16 or 17 or 18 or 19 or 20 or 2
1 or 22 or 23 or 24
A shipping process for sending to a doctor, and at least the adsorption unit in the blood separation device,
By using the blood separation device, components other than blood cell components can be obtained.
Non-blood cell component, which is the blood plasma component or serum component
Receipt to receive the adsorption part that adsorbs a certain non-blood cell component
Perform an inspection using the process and the adsorption unit where non-blood cell components are adsorbed.
Blood testing how to, comprising cormorants and inspection step. 26. The inspecting step is performed by shaking and extracting an adsorbing portion on which a non-blood cell component is adsorbed.
Extraction liquid generation process for obtaining liquid and measurement process for performing predetermined measurement using the obtained extraction liquid
When blood test method according to claim 25, characterized in that it comprises a.