JP2002243726A - Blood separator, and method of testing blood - Google Patents

Abstract

PROBLEM TO BE SOLVED: To easily separate a nonhemocyte component in blood without using a centrifugal separator, to allow use for examination by a mail, and to allow a biochemical item to be measured without any disturbance. SOLUTION: In this blood separator P, a main body part A has an upper main body part 10 and a lower main body part 40, and a separation unit B and an adsorption unit C are arranged in a storage space inside the main body part A. A separation membrane 110 for separating the nonhemocyte component in the blood is provided in the separation unit B, and adsorption filter paper 130 for adsorbing the separated nonhemocyte component is provided in the adsorption unit C. A sheetlike member D constituted of a film is arranged between the upper main body part 10 and the separation unit B. When used, dropping is carried out from a through hole 18 of the upper main body part 10, and the sheetlike member D is extracted thereafter to allow falling-off onto the separation membrane 110.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a blood separation device, and more particularly to a blood separation device capable of quantitatively separating non-blood cell components from blood.

[0002]

2. Description of the Related Art Conventionally, when separating non-blood cell components from blood, centrifugation is performed on the collected blood to separate the blood cell components from the non-blood cell components. That is,
The blood collected from the vein is centrifuged, and measurement is performed by a measuring device using a liquid non-blood cell component, mainly a plasma component, as a sample.

[0003] Here, the non-blood cell component mainly means a plasma component or a serum component, and means a component other than the blood cell component in blood. The blood cell component includes white blood cells, red blood cells, platelets, and the like.

As a method of performing a checkup by mail, conventionally, fingertip blood, that is, blood collected from a finger is dropped on a blood collection filter paper, dried for a predetermined time, put in a bag, and sent to an inspection organization. There is one that performs an inspection.

[0005]

However, in the above-mentioned centrifugal separation method, it is necessary to perform separation by a centrifugal separator.
A centrifugal separator is required. In addition, it requires time and effort for the centrifugal separator. In particular, when performing a medical examination by mail, a centrifugal separator is required on the participant (subject) side, and even if centrifugation can be performed on the participant side, the specimen is in a liquid state. So
There is a problem that the transfer becomes difficult, and the test component of the sample is inactivated or denatured if it remains in a liquid state.

In the method of dropping blood on the blood sampling filter paper described above, since blood adheres to the blood sampling filter paper,
When measuring biochemical items such as T, GPT, cholesterol, etc., it becomes difficult to measure colorimetrically. That is, since the extract obtained from the blood collection filter paper has a red color due to hemoglobin, there is a problem that colorimetry, that is, measurement by a colorimetric method cannot be performed. In addition, unlike biochemical items,
Regarding immunological items, even an extract from blood collection filter paper with blood dropped, that is, an extract having a red color, can be reacted only with the substance to be measured, so that it can be measured in that sense. .

Accordingly, the present invention can easily separate non-blood cell components in blood without using a centrifugal separator, and can be used for postal examinations. It is an object of the present invention to provide a blood separation device that does not hinder measurement.

[0008]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above problems, and firstly, a non-blood cell component which is a component other than a blood cell component from blood. Blood separation device for separating non-blood cell components which are plasma components or serum components (may be referred to as "blood processing device")
A separating portion for separating non-blood cell components, and a first holding portion holding the separating portion, wherein the first holding portion has a plate shape.
A separation unit having a holding unit, an adsorption unit that adsorbs the non-blood cell components separated by the separation unit, and a second holding unit that has a plate shape and is a second holding unit that holds the adsorption unit. A main unit that holds the separation unit and the adsorption unit in a state where the separation unit and the adsorption unit are stacked, and an upper main unit that detachably holds the separation unit. An upper main body for holding the separation unit on the lower surface side of the upper main body so that the separation unit is located below the through hole; A lower body that holds the unit, and a switching member provided between the upper body and the separation unit, wherein the switching member is provided in a closed state that closes a lower end of the through hole in the upper body. Open state with the lower end open And having a switching member for switching, a.

In the blood separation device of the first configuration, blood is dropped into the through hole of the upper main body. At this stage, the switching member is closed so as to close the lower end of the through-hole, so that blood does not drop to the separation section. Next, the lower end of the through hole is opened by the switching member. Then, since the separation unit is held in the upper main body of the main body and the separation is located below the through hole, the blood reaches the separation part, where the blood cell component is captured in the separation part, and only the non-blood cell component is captured. Separates and moves to the lower surface side. Then, since the separation unit and the adsorption unit are held by the main body in a stacked state, the separated non-blood cell component is adsorbed by the adsorption unit. The suction part is shaken and extracted in a liquid such as physiological saline to extract non-blood cell components, which are used for various tests.

Therefore, the centrifugal separation operation is not required when separating the non-blood cell components, and the non-blood cell components can be separated by a simple method. Further, if the adsorbing section on which the non-blood cell component has been adsorbed is dried, it can be stored at room temperature or transported by mail or the like. In addition, since red blood cells are captured by the separation unit, the adsorption unit to which non-blood cell components are adsorbed does not contain hemoglobin in the red blood cell components, so that it does not affect colorimetric quantification, and immunological measurement is not affected. In addition, biochemical measurements are possible. Further, particularly, when dropping blood into the through-hole, the lower end of the through-hole can be closed by the switching member, so that the blood is stored in the through-hole, and then the switching member is opened and the separation unit is opened. Since the blood is dropped, a fixed amount of blood can be dropped to the separation unit.

Note that the first configuration may be the following configuration. That is, from the blood, a non-blood cell component that is a component other than the blood cell component, a blood separation device for separating non-blood cell components that are plasma components or serum components,
A separation unit having a separation unit for separating non-blood cell components, a first plate-shaped main body holding the separation unit, and a plate-shaped first plate-shaped main body; An adsorbing unit having an adsorbing portion for adsorbing the separated non-blood cell components, a second plate-shaped main portion holding the adsorbing portion, and a plate-shaped second plate-shaped main portion; The unit is a main body that holds the separation unit and the adsorption unit in a stacked state, and is an upper main body that detachably holds the separation unit, and has a through hole for dropping blood. An upper body that holds the separation unit on the lower surface side of the upper body, and a lower body that holds the suction unit, such that the separation unit is located below the through hole. Having a main body, between the upper main body and the separation unit. A switching member for switching from a closed state in which the lower end of the through-hole in the upper body is closed to an open state in which the lower end of the through-hole is opened. . It is also conceivable to adopt a configuration in which the switching member is omitted in the first configuration.

Secondly, in the first structure, the separating portion is a sheet-like separating film having a plurality of pores therein, and the diameter of the pores gradually changes in the thickness direction. Characterized by being formed by a separation membrane having the same. Thereby, the blood cell component of the blood is captured, and the non-blood cell component can be separated. When holding the separation unit having the separation membrane in the main body, the separation unit is held with the side having the larger diameter of the pores facing the upper main body.

Thirdly, in the first or second configuration, the adsorbing section is a sheet-shaped filter paper.

Fourthly, in any one of the first to third configurations, the upper main body and the lower main body are connected to each other in the main body, and the upper main body and the lower main body are connected to each other. Is rotatable with respect to the other, and the upper main body and the lower main body have attachment / detachment means for locking each other. Therefore, the state in which the separation unit and the suction unit are held by the main body portion can be fixed by locking the upper main body portion and the lower main body portion by the attaching / detaching means.

Fifth, in any one of the first to fourth configurations, the through hole in the upper main body has an inverted truncated cone shape, and the through hole in the upper main body is provided on the separation unit disposition side in the upper main body. The diameter of the through hole on a certain lower surface side is smaller than the diameter of the through hole on the upper surface side opposite to the lower surface side. Therefore, the opening on the side where the blood is dropped can be made wider, so that the blood can be dropped easily.

Sixth, in any one of the first to fifth configurations, the upper body has an upper surface which is opposite to a lower surface on which the separation unit is disposed, and the upper body has a through hole. In the region where the hole is formed, a protruding portion formed to protrude from other regions is formed, and the through hole is formed in the protruding portion. The provision of the protruding portions makes it possible to easily drop blood.

Seventh, in any one of the first to sixth configurations, the outer periphery of the through hole on the lower surface side of the upper main body portion on the side where the separation unit is provided,
The groove portion is provided in an annular shape. With this groove, an outflow location of the air when the blood falls can be formed, and the blood can be smoothly dropped into the separation part.

Eighthly, in the above-mentioned seventh structure, a communication hole is provided for communicating the groove and the outer surface on the upper surface side of the upper main body. In the eighth configuration, the communication hole is provided,
One of the communication holes is open on the outer surface of the main body, so that the air that is pushed out when the blood falls can be discharged to the outside of the main body, and the blood can be smoothly dropped to the separation part. It is possible to do. In particular, when a protruding portion is provided as in the sixth configuration, it is preferable to open the communication hole at the upper end of the protruding portion.

Ninth, in the seventh or eighth configuration, the separating portion has a circular sheet shape, and the diameter of the separating portion is larger than the diameter of the groove. Features. Thereby, the size of the separation portion can be made sufficiently large.

In a tenth aspect, in any one of the first to ninth configurations, the gap between the lower surface of the upper main body on the side where the separation unit is provided and the separation portion of the separation unit. Is characterized in that a gap is formed. Thus, a space for escaping air can be formed, and in particular, when the above-mentioned grooves and communication holes are formed, air can be efficiently discharged.

Eleventh, in any one of the first to tenth configurations, the lower body is located at a position below the suction unit in a state where the suction unit is held by the lower body. , And a through hole is formed. Thereby, since the air pushed out by the fall of the blood can be exhausted also from the lower main body part side, the separation of the non-blood cell component and the adsorption of the non-blood cell component to the adsorption part can be promoted. It is possible to promote the drying of the non-blood cell component adsorbed on the adsorption section.

A twelfth feature is that, in any one of the first to eleventh configurations, the first holding portion has a plate shape, and the second holding portion has a plate shape. And

In a thirteenth aspect, in any one of the first to twelfth configurations, the first holding portion of the separation unit has an opening, and the separation portion is located at the position of the opening. The separation unit, the lower surface of the separation unit on the suction unit side is formed inside the lower surface of the first holding unit, and a recess is formed in the opening of the lower surface of the separation unit. It is characterized by. With this concave portion, it is possible to form a space in which the suction portion is fitted.

In a fourteenth aspect, in any one of the first to thirteenth configurations, in the suction unit, an opening is provided in the second holding part, and the suction part is provided in the second holding part. It is characterized in that it is provided so as to close the opening. Therefore, the suction section is in a state of protruding from the upper surface of the second holding section, and the suction section can be fitted into a recess formed in the separation unit.

Fifteenth, in any one of the first to twelfth structures, the first holding portion of the separation unit has an opening, and the separation portion is located at the position of the opening. The separation unit, the lower surface of the separation unit on the suction unit side is formed inside the lower surface of the first holding unit, and a concave portion is formed in an opening of the lower surface of the separation unit. In the suction unit, an opening is provided in the second holding unit, and the suction unit is provided on the second holding unit so as to cover the opening, and the separation unit and the suction unit are stacked. In this case, the suction unit is fitted in a recess formed in the separation unit. Therefore, the suction unit is in a state of protruding from the upper surface of the second holding unit, and it is possible to fit this suction unit into the recess formed in the separation unit. It is possible to accurately and closely contact a predetermined position.

In a sixteenth aspect, in any one of the first to fifteenth configurations, a fixing means for fixing the suction unit and a lower main body in the main body is provided. I do. Therefore, when the upper main body and the lower main body are left open, the separating section and the adsorbing section can be reliably separated.

In a seventeenth aspect, in any one of the first to sixteenth configurations, the switching member has a sheet shape, and a part of the switching member is disposed so as to be exposed from the main body. By moving (for example, pulling out) the switching member, the lower end of the through hole in the upper main body can be opened.

Eighteenthly, in the seventeenth configuration, the switching member is provided with an opening for letting the blood stored in the through hole pass therethrough. The size of the diameter of the opening is preferably equal to or less than the size of the diameter on the lower surface side of the through hole.

Nineteenthly, in any one of the first to eighteenth constructions, when the separation unit and the suction unit are stacked and held by the main body, the separation unit in the separation unit The unit and the suction unit of the suction unit are in close contact with each other. Therefore, since the separation unit and the adsorption unit are in close contact with each other, it is possible to contribute to the quantitativeness of the non-blood cell component adsorbed on the adsorption unit.

A twentieth aspect is a blood separation apparatus for separating non-blood cell components, which are non-blood cell components other than blood cell components, and are plasma components or serum components ("Blood processing"). A separation unit for separating non-blood cell components, an adsorption unit for adsorbing the non-blood cell components separated by the separation unit, and a through-hole for dropping blood. And a switching member provided between the main body portion and the separation portion, wherein the lower end portion of the through hole in the main body portion is a switching member provided between the main body portion and the separation portion. A switching member for switching from a closed state to a closed state to an open state in which the lower end of the through hole is opened.

In the twentieth configuration, blood is dropped into the through hole of the main body. At this stage, the switching member is closed so as to close the lower end of the through-hole, so that blood does not drop to the separation section. Next, the lower end of the through hole is opened by the switching member. Then, since the separation portion is located below the through hole, the blood reaches the separation portion, where the blood cell component is captured, only the non-blood cell component is separated, and moves to the lower surface side. Then, the separated non-blood cell component is adsorbed on the adsorption section. The suction part is shaken and extracted in a liquid such as physiological saline to extract non-blood cell components, which are used for various tests.

Therefore, the centrifugal separation operation is unnecessary when separating the non-blood cell components, and the non-blood cell components can be separated by a simple method. Further, if the adsorbing section on which the non-blood cell component has been adsorbed is dried, it can be stored at room temperature or transported by mail or the like. In addition, since red blood cells are captured by the separation unit, the adsorption unit to which non-blood cell components are adsorbed does not contain hemoglobin in the red blood cell components, so that it does not affect colorimetric quantification, and immunological measurement is not affected. In addition, biochemical measurements are possible. Further, particularly, when dropping blood into the through-hole, the lower end of the through-hole can be closed by the switching member, so that the blood is stored in the through-hole, and then the switching member is opened and the separation unit is opened. Since the blood is dropped, a fixed amount of blood can be dropped to the separation unit.

In the twentieth configuration, the switching member has a sheet shape, and a part of the switching member is provided so as to be exposed from the main body, and the switching member is moved (for example, By pulling out), it is preferable that the lower end of the through hole in the upper body can be opened. Further, it is preferable that the switching member is provided with an opening for passing the blood stored in the through hole. In addition, the twentieth
In the above configuration, it is also conceivable to adopt a configuration in which the switching member is omitted.

In a twenty-first aspect, in the twentieth configuration, the separating portion is a sheet-like separation membrane having a plurality of pores therein, and the diameter of the pores gradually changes in the thickness direction. Characterized by being formed by a separation membrane having the same. Thereby, the blood cell component of the blood is captured, and the non-blood cell component can be separated. When holding the separation unit having the separation membrane in the main body, the separation unit is held with the side having the larger diameter of the pores facing the upper main body.

In the twentieth or twenty-first configuration, the through hole in the main body may have an inverted truncated cone shape. Further, on the upper surface side of the main body, a projecting portion is formed in a region where the through hole is formed so as to protrude from other regions, and the through hole is formed in the projecting portion. You may make it.
Further, a groove may be provided in an annular shape on the outer periphery of the through hole in the main body. In addition, a communication hole that communicates the groove with the outer surface on the upper surface side of the main body may be provided. Further, the separating portion may have a circular sheet shape, and the diameter of the separating portion may be larger than the diameter of the groove.

In a twenty-second aspect, in any one of the first to twenty-first configurations, by dropping a predetermined amount or more of blood into the separation section, a substantially constant amount of non-blood cell components is added to the adsorption section. It is characterized by being adsorbed to Therefore, the quantitativeness of the non-blood cell component adsorbed on the adsorption section can be ensured.

In a twenty-third configuration, in any one of the first to twenty-second configurations, the separation unit may be configured to prevent the dropping of a predetermined amount or more of blood from exceeding the water retention capacity of the adsorption unit. Has the ability to separate non-blood cell components. Therefore, the quantitativeness of the non-blood cell component adsorbed on the adsorption section can be ensured.

The following configuration may be adopted in addition to the above configuration. In other words, a blood separation apparatus for separating non-blood cell components, which are components other than blood cell components, such as plasma components or serum components, from blood, and is a separation device for separating non-blood cell components. A separation section formed by a separation membrane having a structure in which a diameter of the pores gradually changes in a thickness direction with a sheet-shaped separation membrane having a plurality of pores therein, and a second section holding the separation section. One plate-shaped main body,
A separation unit having a plate-like shape having an opening, a first plate-shaped main body having a separation portion fixed and held in the opening, and an adsorption portion for adsorbing non-blood cell components separated by the separation portion A second plate-shaped main body holding the suction portion, a second plate-shaped main body having an opening, and a second plate-shaped main body fixedly disposed on the second plate-shaped main body so as to close the opening. A suction unit having a plate-shaped main body, and a main body that holds the separation unit and the suction unit in a state where the separation unit and the suction unit are stacked, and detachably holds the separation unit. In the upper body portion, having a through-hole having a shape of an inverted truncated cone for dropping blood, on the upper surface side opposite to the lower surface side which is the separation unit arrangement side in the upper body portion, In the area where the through hole is formed, the area is larger than in other areas. Out protruding portions formed is formed, the projecting shape portion, the through hole is formed, also,
The separation unit is supported on the lower surface side of the upper main body so that the separation unit is located below the through hole, and the through hole on the lower surface side of the upper main body which is the separation unit disposed side. On the outer periphery, a groove portion is provided in a circumferential shape, and further, the upper body portion provided with a communication hole for communicating the groove portion and the outer surface on the upper surface side of the upper body portion, and the suction unit. A lower body part to be held, the lower body part having a through-hole formed at a position below the suction part in a state where the suction unit is held by the lower body part; and The lower main body is connected to the lower main body, and the upper main body is rotatable with respect to the lower main body. The upper main body and the lower main body are provided with attaching / detaching means for engaging with each other. The main unit and the upper unit A sheet-like member provided between the slots, the sheet-like member being in an open state in which the lower end of the through hole is opened from a closed state in which the lower end of the through hole in the upper main body is closed. It may be a blood separation device that performs the operation.

A twenty-fourth blood test method for performing a blood test, comprising: sending a blood separation device having any one of the first to twenty-third configurations to a patient; At least the adsorption unit in the device, by using the blood separation device, the non-blood cell component which is a component other than the blood cell component, the non-blood cell component which is a plasma component or a serum component is adsorbed by the non-blood cell component The method is characterized by having a receiving step of receiving and a test step of performing a test using an adsorption section to which a non-blood cell component is adsorbed.

According to a twenty-fifth aspect, in the twenty-fourth aspect, the testing step comprises: extracting an extract by shaking and extracting an adsorbing portion to which the non-blood cell component is adsorbed;
And performing a predetermined measurement using the obtained extract.

Accordingly, since the predetermined measurement is performed using the extract obtained by shaking and extracting the adsorption section to which only the non-blood cell component is adsorbed, accurate measurement can be performed even in the colorimetric method. It becomes possible.

A twenty-sixth method is a blood test method for performing a blood test, which is a non-blood cell component, which is a component other than a blood cell component, obtained by separating a blood cell component from blood, Alternatively, an extract is formed by shaking and extracting an adsorbent (adsorbing section) on which a non-blood cell component, which is a serum component, is adsorbed, and a predetermined measurement is performed using the obtained extract. And a step.

Therefore, since the predetermined measurement is performed using the extract obtained by shaking and extracting the adsorbent on which only the non-blood cell component is adsorbed, accurate measurement can be performed even in the colorimetric method. It becomes possible.

In the twenty-seventh aspect, the twenty-fifth or the twenty-sixth is described.
Wherein the predetermined measurement is a measurement performed by a colorimetric method.

In the twenty-eighth, from the twenty-fourth to the twenty-seventh
In any of the above configurations, the adsorbing portion or adsorbent on which the non-blood cell component is adsorbed is a sheet-like separation membrane having a plurality of pores therein, and the diameter of the pores gradually changes in the thickness direction. The non-blood cell component thus separated is adsorbed by dropping blood onto a separation membrane having a structure.

[0046]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to the drawings. The blood separation device (also referred to as a “blood processing device”) P according to the present invention is for separating non-blood cell components in blood, that is, components other than blood cell components in blood, from blood. Is for separating plasma components, serum components and the like. As shown in FIGS. 1 to 4, the blood separation device P includes a main body A, a separation unit B, a suction unit C, and a sheet-shaped member D.

First, the main body A will be described with reference to FIGS. The main body A has a case shape as a whole, and has a space for accommodating the separation unit B and the suction unit C therein. This body A is
It is generally formed of a synthetic resin material. Specifically, it is formed of a PP resin such as polypropylene.

The main body A has an upper main body 10 and a lower main body 40. First, the upper body 10
Plate-like part 12, frame-like part 14, protruding part 16, through-hole 1
8, a groove 20, a protruding part 24, and a locking part 26.

Here, the plate portion 12 has a substantially rectangular plate shape. The upper surface 12a and the lower surface 12b of the plate portion 12 are parallel to each other. In addition, the frame-shaped portion 1
Numeral 4 protrudes from the outer periphery of the plate portion 12 to the lower surface side, that is, to the lower main body portion 40 side. That is, the frame portion 14 protrudes from the outer peripheral region along four sides of the lower surface 12b of the plate portion 12, and includes the frame portion 14a, the frame portion 14b, the frame portion 14c, and the frame portion 14d. have. A notch 14-1 is provided in a part of the frame-shaped portion 14.
Is provided. That is, the elongated cutout portion 14-1 is provided near the center of the frame portion 14c adjacent to the lower main body portion 40 via the connecting portion 60. This notch 1
4-1 is for arranging a sheet-like member D described later.

A pair of claw portions 15 are formed to protrude inside the frame portion 14a, and a pair of claw portions 15 are formed to protrude inside the frame portion 14c. The claw portion 15 is for fixing the separation unit B, and sandwiches the separation unit B between the plate unit 12 and the separation unit B.
The separation unit B is fixed.

The protruding portion 16 is provided on the upper main body 10.
, That is, on the upper surface 12a of the plate-shaped portion 12, that is, on the front surface, and has a substantially ring shape. The outer periphery of the projection 16 in a plan view is circular, and the outer side surface is inclined. Further, a through hole 18 is provided at the center of the protruding portion 16. This through hole 1
8 is an upper surface of the upper main body 10, specifically, a protrusion 16
Penetrates from the upper portion to the lower surface (that is, the rear surface) of the upper main body 10, and the side surface of the through hole 18 is formed in an inclined and tapered shape, and the side surface of the through hole 18 is 1
2 are symmetrical about an axis in a direction (Z direction) perpendicular to the plane formed by the upper surfaces 12a of the two. That is, the through hole 18
It has an inverted truncated cone shape. Thereby, both the upper surface side and the lower surface side of the through hole 18 are opened in a circular shape. That is, the opening 18-1 on the upper surface 12a side of the through hole 18 and the opening 18-2 on the lower surface 12b side of the through hole 18 are circular. Also, the opening 18-1 is
Greater than -2. That is, the upper surface 1 of the through hole 18
When the diameter of the opening 18-1 on the 2a side is a and the diameter of the opening 18-2 on the lower surface 12b side of the through hole 18 is b, a> b. That is, the upper main body 10
Is smaller than the diameter of the through-hole 18 on the upper surface side opposite to the lower surface side. In addition, the protrusion 16
Is formed in a substantially planar shape, and has a ring shape.

That is, in the area where the through hole 18 is formed, another area, that is, a projecting portion 16 which is formed so as to protrude from the other area of the upper surface of the upper main body 10 is formed. The through hole 18 is formed in the protruding portion 16.

The groove 20 is provided on the lower surface of the upper main body 10, specifically, on the lower surface of the plate-like portion 12, that is, on the back surface. ), That is, in a circumferential shape. This groove 2
The vertical cross-sectional shape of 0 is square. This groove 20
, That is, between the ceiling and the upper surface of the protruding portion 16, a plurality of (specifically, four) insertion holes (communication holes) 2
2 are provided. That is, the insertion hole 22 penetrates between the protrusion 16 and the groove 20, is provided along the Z direction, and communicates with the outer surface on the upper surface side of the upper main body 10.

The protruding portion 24 is provided on the upper main body 10.
, Specifically, on the lower surface of the plate-shaped portion 12, a pair is provided. That is, they are arranged on both sides in the X direction with the through hole 18 and the groove 20 interposed therebetween. This protrusion 24
It has a substantially cylindrical shape, and a cylindrical hole 25 is provided therein. A projecting portion 46 described later is fitted in the hole 25.

The locking portion 26 is a detachable member for detachably fixing the upper body portion 10 and the lower body portion 40 in a closed state, and is provided on the frame-shaped portion 14. The upper body 10 and the lower body 40 can be fixed in a closed state together with the stopper 50.

Further, a protruding piece 28 is formed at a corner on the back surface of the plate-shaped portion 12, and if the disposing unit B is not arranged correctly, the protruding piece 28 By disturbing the arrangement of the separation unit B, the separation unit B can be set in the correct arrangement direction. In addition, 4-4 of the plate-like portion 12 in the upper main body 10.
An elongated rectangular opening 30 is provided near one corner (see FIG. 7).

Next, the lower body section 40 includes a plate-shaped section 42,
It has a mesh projection 44, a projection 46, a through hole 48, and a locking part 50.

Here, the plate portion 42 has a substantially rectangular plate shape, and the upper surface 42a and the lower surface 42 of the plate portion 42 are formed.
b are parallel to each other. The size and shape of the plate portion 42 in plan view are smaller than the plate portion 12 of the upper main body 10.

The mesh projecting portion 44 is provided on the upper surface 42a of the plate-like portion 42 and, as shown in FIG. The mesh protrusions 44 are formed to maintain the rigidity of the lower main body 40. The shape of the mesh protrusion 44 is formed parallel to the long side and the short side of the plate-like portion 42, and the central portion thereof has a double shape as shown in FIGS. It is formed in a circular shape.
That is, the circular protrusion 49-1 and the circular protrusion 49-1
By forming a circular projection 49-2 having a larger diameter than that, it projects in a double circle.

The projection 46 is provided in a pair on the upper surface of the plate-like portion 42. That is, they are arranged on both sides in the X direction with the through hole 48 interposed therebetween. This protrusion 46
Has a columnar shape, and is provided at a position corresponding to the position of the hole 25 when the upper main body 10 is closed with respect to the lower main body 40. That is, when the upper main body 10 is closed with respect to the lower main body 40, the protrusion 46 is fitted into the hole 25 and enters.

The through hole 48 is provided substantially at the center of the lower main body 40. That is, this through hole 48
Inside the circular protrusion 49-1, the circular protrusion 49-
It is provided at the center position of the range surrounded by 1. The through hole 48 extends from the upper surface 42a of the lower main body 40 to the lower surface 42a.
This is a through hole penetrating to b, and has a columnar shape. The through hole 48 is provided at a position corresponding to the position of the through hole 18 when the upper main body 10 is closed with respect to the lower main body 40. That is, when the upper main body 10 is closed with respect to the lower main body 40, the circle formed by the through hole 18 and the circle formed by the through hole 48 are concentric in plan view. . That is, the through hole 18
And the longitudinal (Z direction) axis of the through-hole 48 coincides with the longitudinal direction (Z direction). When the suction unit C is held by the lower main body 40, the through hole 48 is located below the suction filter paper 130 of the suction unit C. At this time, the absorption filter paper 1
The center of 30, ie, the axis, and the axis of the through-hole 48 coincide or substantially coincide. The diameter of the through hole 48 is smaller than the diameter of the opening 18-2 on the lower surface side of the upper main body 10 of the through hole 18. That is, when the diameter of the through hole 48 is c, b> c (see FIGS. 8 and 1).
2). FIG. 8 is a sectional view of the main body A,
The cutting position is the same as the position XX in FIG. FIG. 12 is also a cross-sectional view of a main part of the blood separation device P, and its cutting position is the same as the position XX in FIG.

The locking portion 50 is a detachable member for detachably fixing the upper body portion 10 and the lower body portion 40 in a closed state, and is provided at the front end of the plate-like portion 42.
The upper main body 10 and the lower main body 40 can be fixed in a closed state together with the other locking portion 26. That is, when the upper main body 10 and the lower main body 40 are closed, the locking portion 26 and the locking portion 50 are locked to each other and connected. In addition, the locking portion 26 in the coupled state
The locking portion 26 and the locking portion 50 are operated by operating the locking portion 50 and the locking portion 50 with a predetermined force or more so as to separate them vertically.
And the upper main body 10 and the lower main body 40 can be opened. The locking portion 26 and the locking portion 50 function as the attaching / detaching means.

A plurality of grooves 52 are formed on the lower surface 42b of the lower main body 40.

The upper body 10 and the lower body 4
0 is connected by the connecting portion 60. That is, a pair of connecting portions 60 are provided at a predetermined interval between the end along the long side of the upper main body 10 and the end along the long side of the lower main body 40. The connecting portion 60 is formed to be easily bent, so that the upper main body 10 and the lower main body 40 can be easily opened and closed. That is, one of the upper main body 10 and the lower main body 40 is rotatable with respect to the other. When the upper main body 10 and the lower main body 40 are closed, a storage space is formed inside the main body A. This storage space is a space for storing the separation unit B and the suction unit C.

Next, the separation unit B will be described. As shown in FIG. 9, the separation unit B has a main body (first holding unit, first plate-shaped main body) 100 and a separation membrane (separation unit) 110.

The main body 100 has a substantially plate shape, and its size and shape are formed so as to be housed in a storage space formed in the main body A. The main body 100 has an upper plate-shaped portion 102 and a lower plate-shaped portion 104, and the upper plate-shaped portion 102 and the lower plate-shaped portion 104 are bonded by an adhesive layer 106. The adhesive layer 106 is provided over the entire area where the upper plate-shaped portion 102 and the lower plate-shaped portion 104 are in contact. The upper plate-shaped portion 102 and the lower plate-shaped portion 104 are formed of a synthetic resin material, specifically, a PET-based resin such as polyethylene terephthalate (may be polyethylene terephthalate; the same applies hereinafter).

The upper plate-like portion 102 has a rectangular shape with a triangular cutout 100K formed at one corner thereof, and a large-diameter circular opening 103 at the center.
And a small-diameter circular opening on the left and right sides of the circular opening 103. The large-diameter circular opening 103 forms a space for disposing the separation membrane 110. In the main body 100, the small-diameter circular openings form openings 108 a, 108 b for fitting into the protruding portions 24 together with the subtotal circular openings provided in the lower plate-shaped portion 104. The notch 100K is for specifying the direction of the separation unit B.

The shape of the lower plate portion 104 has the same outer shape as that of the upper plate portion 102.
It has a large-diameter circular opening 105, and has a small-diameter circular opening on the left and right sides of the circular opening 105. The size of the large-diameter circular opening 105 located at the center is as described above. It is slightly smaller than the circular opening 103. In addition, the size and formation position of the small-diameter circular opening are determined by the upper plate-like portion 102.
The openings 108a and 108b are formed together with the small-diameter circular openings provided in the upper plate-shaped portion 102. The main body 100 holds the separation membrane 110.

The separation membrane 110 has a circular sheet shape and is provided in the circular opening 103. More specifically, in the adhesive layer 106, the adhesive layer 106 is exposed inside the circular opening 103 in a ring shape.
The adhesive layer 106 is fixed to the main body 100 by being bonded to a ring-shaped exposed portion. Note that the adhesion between the separation membrane 110 and the adhesive layer 106 is performed by the adhesive layer 112.
Done through. The adhesive layers 106 and 112 are made of, for example, a double-sided adhesive tape.

The diameter e of the separation membrane 110 is equal to the main body A.
Is formed to be larger than the diameter d of the groove portion 20 in the upper main body portion 10. That is, as shown in FIG.
It has become.

The separation membrane 110 is formed of polysulfone as a raw material and has a large number of pores inside. The thickness of the separation membrane 110 is about 300 μm, and the pore diameter of the pores is 40 to 60 μm on the front side, becomes smaller toward the back side, and is about 2 μm on the back side. In FIG. 9C, the diameter of the pores is large on the upper side of the separation membrane 110, and the diameter of the pores is small on the lower side. That is, blood cell components are captured in a so-called sparse layer near the front surface, and non-blood cell components are filtered in a so-called dense layer near the back surface. The separation membrane 110 is for separating non-blood cell components such as plasma components and serum components.

The separation membrane 110 has the ability to separate non-blood cell components in an amount exceeding the water retention capacity of the adsorption filter paper 130 when a predetermined amount or more of blood is dropped.

As shown in FIG. 9, a concave portion 114 is formed on the lower surface of the separation film 110. That is, the lower surface of the separation membrane 110 is formed inside the lower surface of the main body 100, that is, the lower surface of the lower plate portion 104, and the concave portion 114 is formed on the lower surface of the separation unit B. When the separation unit B and the suction unit C are stacked, the suction filter paper 130 of the suction unit C is fitted into the recess 114. Also, the upper plate-like portion 1
Since the thickness of the separation membrane 110 is slightly smaller than the thickness of the separation unit 02, the portion of the circular opening 103 of the separation unit B is also
A recess 116 having a small depth is formed. Thereby, even when the separation unit B is held by the upper main body 10 and the upper surface of the separation unit B is in contact with the lower surface of the upper main body 10, the lower surface of the upper main body 10 and the separation film 110 are separated from each other. A gap is formed between them.

Next, the suction unit C will be described.
As shown in FIG. 10, the suction unit C includes a main body (second
It has a holding section, a second plate-shaped main body section) 120 and an adsorption filter paper (adsorption section, adsorbent) 130.

The main body 120 has a substantially plate shape, and its size and shape are formed so as to be housed in a storage space formed in the main body A. Specifically, as shown in FIG. 10, a rectangular notch 1 is formed at one corner of the rectangular shape.
It has a plate shape having a shape of 20K, has a large-diameter circular opening 122 at the center, and small-diameter circular openings 124a and 124b on the left and right sides of the circular opening 122. . The circular openings 124a and 124b are
6 is formed in a size and a shape that can be fitted. Also,
The main body 120 has a size slightly smaller than the main body 100 of the separation unit B, as shown in FIG. The main body 120 is made of a synthetic resin material, specifically,
It is formed of a PET resin such as polyethylene terephthalate. The notch 120K is provided in the separation unit B.
This is for specifying the direction of. The main body 120 holds the adsorption filter paper 130.

The adsorption filter paper 130 has a circular sheet shape and is disposed on the upper surface of the main body 120 so as to close the circular opening 122. That is, the main body 12
0 and the adsorption filter paper 130 are adhered via an adhesive layer 132. The adhesive layer 132 has a ring shape. Further, the suction filter paper 130 is disposed such that the center of the circle formed by the suction filter paper 130 and the center of the circle formed by the circular opening 122 match.

In this embodiment, the filter paper is taken as an example of a member that adsorbs non-blood cell components. However, the present invention is not limited to the filter paper, and other adsorbing members may be used.

As shown in FIG. 10, an adhesive layer 134 and a release sheet 136 are provided on the rear surface of the main body 120. By peeling off the release sheet 136, the adhesive layer 134 Can be exposed. The adhesive layer 134 corresponds to the “fixing means for fixing the suction unit and the lower main body in the main body”.

The adhesive layer 132 and the adhesive layer 134
For example, it is formed by a double-sided adhesive tape.

In particular, the thickness when the separation unit B and the suction unit C are overlapped is substantially the same as the thickness f of the storage space of the main body A (see FIG. 8). The suction unit C is set in the main body A,
In the state as shown in FIG. 2, the separation unit B and the suction unit C are fixed in the main body A in the vertical direction. Since the separation unit B is fitted to the protruding portion 24 and the suction unit C is also fitted to the protruding portion 46, the separation unit B is fixed in the main body portion A even in the plane direction.

The sheet member D has a substantially strip-like sheet shape and is formed of a synthetic resin film, specifically, a PET resin film such as polyethylene terephthalate. This sheet-shaped member D has a base end 200 and a front end 202 as shown in FIG. The width h of the base end portion 200 of the sheet-shaped member D is
4 is formed to be equal to or less than the width of the notch 14-1.
Thereby, in the assembled blood separation device P, the sheet-shaped member D can be disposed in a state of protruding from the main body portion A, and it is possible to pull out the sheet-shaped member D after dropping blood. Become. Further, this sheet-shaped member D
The width i of the distal end portion 202 is formed smaller than the width h of the proximal end portion, and the distal end portion 202 has a substantially square shape with rounded corners formed at its two corners. I have. The width i of the tip 202 is formed larger than the diameter e of the separation membrane 110 (see FIG. 12 and the like). That is,
i> e. That is, this sheet-shaped member D
When the sheet-shaped member D is disposed in the blood separation device P, it has a size that can completely cover the circular opening 103 in the separation unit B. In addition, when the sheet-shaped member D is disposed in the blood separation apparatus P, the sheet-shaped member D is formed so as to cover the opening 18-2 on the lower surface side of the upper main body 10 as a matter of course. The sheet-shaped member D functions as the “switching member for switching from the closed state in which the lower end of the through hole in the upper main body is closed to the open state in which the lower end of the through hole is opened”.

The steps of assembling the blood separating apparatus P having the above configuration will be described. First, the separation unit B, the suction unit C, and the sheet-like member D are set on the main body A. That is, the sheet-shaped member D is disposed on the lower surface of the upper main body 10 of the main body A, that is, on the rear surface, and the separation unit B is further disposed on the lower surface of the upper main body 10. In arranging the sheet-shaped member D, the front end 202 of the sheet-shaped member D is connected to the opening 18 of the upper main body 10.
-2 and a position to close the groove 20. More specifically, it is preferable that the center position of the leading end 202 of the sheet-shaped member D coincides with the center position of the opening 18-2. In addition, the base end 200 continuing from the front end 202 of the sheet-shaped member D passes through the notch 14-1 in the upper main body 10 and reaches the outside of the main body A.

The openings 108a and 108 of the separation unit B are arranged such that the upper plate 102 of the separation unit B faces the upper body 10 side.
The separation unit B is held by the upper main body 10 and fixed to the main body A by inserting the protruding portion 24 protruding from the lower surface of the upper main body 10 into the b. Then, the separation unit B is connected to the upper main body 10.
Is stored in the space on the back side of the.

The suction unit C is held by the lower main body 40 with the side on which the suction filter paper 130 is provided facing upward. That is, the opening 1 of the suction unit C
By inserting a protrusion 46 protruding from the upper surface of the lower main body 40 into the suction unit C
Is fixed to the main part A. At that time, the release sheet 136 provided on the lower surface of the suction unit C is peeled off, and the projection 46 is inserted through the release sheet 136 and set on the lower main body 40. Therefore, when the suction unit C is set on the lower main body 40, the lower surface of the lower main body 4 is
0 is fixed by bonding to the upper surface.

As described above, when the setting of the separation unit B, the suction unit C, and the sheet-like member D is completed, the upper main body 10 is rotated toward the lower main body 40, and the upper main body 10 and the lower main body 10 are rotated. The main body 40 is closed. Then
The locking portion 26 and the locking portion 50 are fixed to each other, and the closed state is fixed. As described above, the assembly of the blood separation device P is completed.

When the assembly of the blood separation device P is completed, the blood separation device P is as shown in FIG. 2, FIG. 12 and the like. It is in a state of being tightly stored in the space in the part A. That is, at least the central portion 10C of the lower surface (back surface) of the upper main body 10 (this central portion 10C is a region including the outer portion of the groove 20 as shown in FIG. 5).
And the sheet-like member D are in close contact with each other.
And the upper surface of the separation unit B are in close contact with each other. That is, the upper surface of the upper plate-shaped portion 102 of the separation unit B and the lower surface of the sheet-shaped member D are in close contact with each other.

The separation membrane 1 is located below the through-hole 18.
10 are located. At this time, the center of the circular shape formed by the through-hole 18 coincides with or substantially coincides with the center of the separation membrane 110.

Further, as shown in FIG. 11, the suction filter paper 130 in the suction unit C is
14, the separation membrane 110 and the adsorption filter paper 130 are in close contact with each other. That is, the adsorption filter paper 130 is in close contact with and under the separation membrane 110. At this time, the center of the separation membrane 100 coincides with or substantially coincides with the center of the adsorption filter paper 130.

In FIG. 2, the sheet-like member D is drawn in a straight line in order to prevent the drawing from being difficult to see, and for the same reason, in the separation unit B,
Configurations other than the separation membrane 110 are drawn integrally,
In the suction unit C, components other than the suction filter paper 130 are integrally drawn. Also in FIG. 12, the sheet-shaped member D is drawn by a straight line.

The use state of the blood separation device P having the above configuration will be described. First, the blood separation device P of the present embodiment is sent by mail or the like to a patient from a source such as a testing institution. At this time, the blood separation device P is stored in a storage container Q as shown in FIG.
The storage container Q is further put in a bag such as a plastic bag and sent. The storage container Q has a storage portion having an open top, and specifically, a storage portion 3 having a substantially square shape in plan view.
00 and storage sections 310 and 312 which are elongated and rectangular in plan view.
have. The storage section 300 is a storage section for storing the blood separation device P, and has a size and a shape that can be stored with the main body section A opened. Further, the storage section 310 and the storage section 312 are spaces for storing accessories such as a lancet, a bandage, and liquor wool. The lancet is an instrument for collecting blood from a fingertip or the like. That is, when sending from the shipping source to the examinee, the blood separating device P is stored in the storage unit 300 in this storage container Q, and the lancet, the bandage, and the alcohol wool are stored in the storage unit 31.
0, 310 and then stored in a bag and sent. In addition,
In FIG. 13, since the state when the patient is sent from the examinee to the testing institution is shown, the upper main body 10 and the lower main body 40 in the main body A are in the open state, but are sent from the sender to the examinee. In such a case, it is stored in the storage unit 300 in a state as shown in FIG.

The examinee who has received the blood separation device P and the accessories spots blood obtained from a fingertip using a lancet or the like. At this time, the blood is spotted to such an extent that the dropped blood fills the inside of the through hole 18 of the upper main body 10. At that time, the protrusion 1
6, the upper body 10 around the opening 18-1 on the upper surface 12a side of the through hole 18.
Since it protrudes from the flat area, the dropping of blood becomes easy. The through-hole 18 is tapered, and the opening 18-1 on the upper surface 12a side is larger than the opening 18-2 on the lower surface 12b side, so that blood is easily dropped. At this stage, since the sheet-shaped member D closes the opening 18-2 on the lower surface 12 b side of the through hole 18, blood does not move below the lower end of the through hole 18.

When blood is dripped to fill the inside of the through hole 18, the sheet member D is removed. That is, in the sheet-shaped member D, the portion exposed to the outside is pulled out, and the sheet-shaped member D is removed.

Then, the blood filling the inside of the through-hole 18 is dropped on the surface side of the separation membrane 110, and gradually the separation membrane 1
10 moves downward. At this time, blood cell components in blood are captured by a layer on the surface side of the separation membrane 110, and only non-blood cell components such as plasma components and components such as serum are separated from the separation membrane 110.
To reach the adsorption filter paper 130. That is, only the non-blood cell component is adsorbed on the adsorption filter paper 130.

In this case, the lower surface 12b of the upper body 10
Is provided around the opening 18-2 of the first hole, and the insertion hole 22 communicating with the groove 20 is provided.
Since the air existing above the zero and inside the separation membrane 110 is pushed out smoothly, it is possible to smoothly drop the blood onto the separation membrane 110 and shake the blood inside the separation membrane 110.

Further, the size of the separation film 110 is
8, since the diameter e of the separation membrane 110 is larger than the diameter d of the groove 20, the blood dropped on the separation membrane 110 is separated in plan view. It does not protrude from the film 110.

The adsorption filter paper 130 is provided in the separation unit B.
Since the filter paper 130 is disposed in the concave portion 114, the disposition position of the adsorption filter paper 130 in plan view does not deviate from the separation membrane 110, and the adsorption filter paper 130 is accurately disposed at a predetermined position below the separation membrane 110. Becomes possible. Therefore, the separated non-blood cell component can be reliably captured by the adsorption filter paper 130.

Further, since the through hole 48 is provided in the lower main body 40, the adsorption of non-blood cell components is further promoted. That is, since the air existing in the traveling direction of the falling blood escapes through the through hole 48, the transfer of the non-blood cell component from the separation membrane 110 to the adsorption filter paper 130 is promoted as compared with the case where the through hole 48 is not provided. Also, when drying the adsorptive filter paper 130 adsorbing the non-blood cell components, the through holes 4
8 further promotes drying. The blood separation device P
In the method, a substantially constant amount of non-blood cell components is adsorbed on the adsorption filter paper by dropping a predetermined amount or more of blood onto the separation membrane 110.

As described above, when the adsorption of the non-blood cell component is completed, the upper main body 10 and the lower main body 40 in the main body A are opened after a predetermined time has elapsed. Then, since the separation unit B is fixed to the upper main body 10 and the suction unit C is fixed to the lower main body 40, the separation unit B and the suction unit C are separated, and the separation membrane 1 is separated.
10 and the adsorption filter paper 130 are separated.

Then, the blood separation device P is stored in the storage container Q and sent to the inspection organization. That is, as shown in FIG.
With the separation unit B and the adsorption unit C opened 180 degrees, the blood separation device P is stored in the storage container Q, and the storage container Q is stored in a bag, and then sent to a testing organization by mail or the like. To In the state shown in FIG.
Since the separation membrane 110 and the adsorption filter paper 130 are stored in the storage container Q in a separated state, the non-blood cell component adsorbed on the adsorption filter paper 130 is affected by the blood cell component adhering to the separation membrane 110. There is no.

In the laboratory, the suction filter paper 130 is taken out of the sent blood separating apparatus P, and the suction filter paper 130 is shaken and extracted to obtain an extract. The adsorption filter paper 130 corresponds to the “adsorbent to which the non-blood cell component which is a component other than the blood cell component and which is a plasma component or a serum component is adsorbed”. In addition, the step of obtaining the extract corresponds to the above-mentioned “extract forming step” in the blood test method of the present invention. That is, the adsorption filter paper 130 is put into a liquid such as a physiological saline solution and shaken and extracted. Adsorption filter paper 13
Since only non-blood cell components are adsorbed to 0, the obtained extract is substantially transparent. This extract is used as a dilution of a normal plasma sample for measurement in various tests.
That is, this corresponds to the “measurement step”.

When measuring biochemical items, measurement is mainly performed by a colorimetric method. That is, reagents are added one after another to the obtained extract to form a color, and the measurement is performed based on the density of the color. At this time, the adsorption filter paper 13
Since only non-blood cell components are adsorbed to 0 and the resulting extract does not contain red components such as hemoglobin, color interference does not occur, and accurate measurement by colorimetry can be performed. It is possible to do.

Unlike the biochemical items, the immunological items can be measured using an extract obtained by shaking and extracting the adsorption filter paper 130.
Examples of the immunological items include PSA (prostate specific antigen) used for screening for prostate cancer and measurement of pepsinogen used for screening for gastric cancer.

As described above, according to the blood separation apparatus P of the present embodiment, the centrifugal separation operation is unnecessary when separating the non-blood cell components, and the plasma can be quantitatively separated by a simple method. . In addition, adsorption filter paper 1 adsorbing non-blood cell components
If 30 is dried, it can be stored at room temperature or transported by mail or the like. Further, since red blood cells are captured by the separation membrane 110, the adsorption filter paper 13 adsorbing non-blood cell components is used.
Since 0 does not include hemoglobin in the red blood cell component, it does not affect colorimetric quantification, and enables biochemical measurement in addition to immunological measurement.

In the above description, the sheet-like member D is described as having the shape shown in FIG. 3, but is not limited to this. For example, the sheet-like member D may have a shape and size as shown in FIG. Is also good. In other words, the base end 200 is
, But the tip 202
Has a circular opening 204 on the tip side. In FIG. 14, the width i of the tip 202
Is smaller than the width h of the base end portion 200 and the through hole 1
8 are formed slightly larger than the diameter of the opening 18-2. The size of the diameter of the opening 204 is determined by the size of the through hole 18.
Is smaller than the diameter of the opening 18-2.

When setting the sheet-like member D, as shown in FIG.
Is arranged near the through hole 18. However, of course, the leading end 20 of the sheet-shaped member D
2 needs to close the lower end of the through hole 18.

After the blood is dropped into the through hole 18, the sheet-like member D is pulled so that the opening 204 is located at the position of the through hole 18. Then, blood drops through the opening 204 and reaches the separation membrane 110. As described above, in this case, since the sheet member D is provided with the opening 204, when the sheet member D is set on the main body A, the opening 204 is provided.
Is disposed in the vicinity of the through hole 18, the sheet-like member D
The blood can be drawn out only a short distance, so that it is possible to minimize the bleeding of blood between the upper body 10 of the body A and the sheet-shaped member D. That is, when the sheet-shaped member D is pulled out and the distance to be drawn out is long,
The amount of blood oozing increases by that much, but if the configuration shown in FIG. 14 is used, the distance for pulling out the sheet-like member D can be shortened, so that the blood can be absorbed by the upper body 10 of the main body A and the sheet-like member D. Bleeding out during can be minimized.

In the above description, in the main body A, the upper main body 10 and the lower main body 40 are
0, and are described as being rotatable with respect to each other. However, the present invention is not limited to this. For example, the upper main body 10 and the lower main body 40 are separated from each other so as to be detachable. Is also good.

[0108]

According to the blood separation device according to the present invention,
A centrifugal separation operation is not required when separating non-blood cell components, and non-blood cell components can be separated by a simple method. Further, if the adsorbing section on which the non-blood cell component has been adsorbed is dried, it can be stored at room temperature or transported by mail or the like. In addition, since red blood cells are captured by the separation unit, the adsorption unit to which non-blood cell components are adsorbed does not contain hemoglobin in the red blood cell components, so that it does not affect colorimetric quantification, and immunological measurement In addition, biochemical measurements are possible. Also,
In particular, when dropping blood into the through-hole, the lower end of the through-hole can be closed by the switching member, so that the blood is stored in the through-hole, and then the switching member is opened to put the blood into the separation unit. Since the blood is dropped, a fixed amount of blood can be dropped to the separation unit.

Further, according to the blood test method of the present invention, a predetermined measurement is performed using an extract obtained by shaking and extracting a paper portion to which only non-blood cell components are adsorbed. Also, the measurement can be performed accurately.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a configuration of a blood separation device according to an embodiment of the present invention.

FIG. 2 is a sectional view taken along line XX in FIG.

FIG. 3 is a plan view showing a configuration of a blood separation device and a sheet-like member according to an embodiment of the present invention.

FIG. 4 is a perspective view showing a state during the assembly of the blood separation device according to the embodiment of the present invention.

FIG. 5 is a perspective view showing a main body in the blood separation device.

FIG. 6 is a plan view showing a state in which an upper main body and a lower main body in the blood separation device are opened by 180 degrees.

FIG. 7 is a bottom view showing a state where the upper main body and the lower main body in the blood separation device are opened by 180 degrees.

FIG. 8 is a sectional view of a main body in the blood separation device.

9A and 9B are diagrams illustrating a configuration of a separation unit, wherein FIG. 9A is a plan view, FIG. 9B is a bottom view, and FIG.
FIG. 3 is a sectional view taken along line YY of FIG.

FIG. 10 is a diagram showing a configuration of a separation unit, and FIG.
Is a plan view thereof, (b) is a bottom view thereof,
(C) is a ZZ sectional view of (a).

FIG. 11 is a sectional view of a main part showing a state in which a separation unit and an adsorption unit are stacked.

FIG. 12 is a sectional view of a main part of a blood separation device according to an embodiment of the present invention.

FIG. 13 is a perspective view showing a storage state of the blood separation device in a storage container.

FIG. 14 is a plan view showing a configuration of a blood separation device and a sheet-like member according to an embodiment of the present invention.

[Explanation of symbols]

 P Blood separation device A Main body B Separation unit C Suction unit D Sheet member 10 Upper main body 18, 48 Through hole 20 Groove 22 Insertion hole 24, 46 Projecting portion 26, 50 Locking portion 40 Lower main body 100, 120 Main body Part 102 Upper plate-like part 104 Lower plate-like part 110 Separation membrane 130 Adsorption filter paper 200 Base end 202 Tip 204 Opening

Claims (28)

[Claims] 1. A blood separation apparatus for separating non-blood cell components, which are components other than blood cell components, from blood, such as plasma components or serum components, wherein the non-blood cell components are separated. A separating unit having a separating unit, a first holding unit that holds the separating unit, and an adsorbing unit that adsorbs non-blood cell components separated by the separating unit.
A suction unit having a second holding unit that holds the suction unit; and a main body unit that holds the separation unit and the suction unit in a state where the separation unit and the suction unit are stacked. An upper body that detachably holds the unit, has a through hole for dropping blood, and the lower part of the upper body so that the separating portion is located below the through hole. A main body having an upper main body that holds the separation unit and a lower main body that holds the suction unit; and a switching member provided between the upper main body and the separation unit. A switching member for switching from a closed state in which the lower end of the through hole is closed to an open state in which the lower end of the through hole is opened. 2. The method according to claim 1, wherein the separation section is formed of a sheet-like separation membrane having a plurality of pores therein, the separation membrane having a structure in which the diameter of the pores gradually changes in the thickness direction. The blood separation device according to claim 1, wherein 3. The blood separation device according to claim 1, wherein the suction unit is a sheet-shaped filter paper. 4. In the main body, the upper main body and the lower main body are connected, and one of the upper main body and the lower main body is rotatable with respect to the other, and the upper main body and the lower main body are connected to each other. The blood separation device according to claim 1, wherein the main body has detachable means for locking each other. 5. The through-hole in the upper body has an inverted truncated cone shape, and the diameter of the through-hole on the lower surface of the upper body on the side where the separation unit is provided is opposite to the lower surface. The blood separation device according to claim 1, wherein the diameter is smaller than a diameter of the through hole on the upper surface side. 6. On the upper surface side opposite to the lower surface side where the separation unit is provided in the upper main body portion, the upper body portion is formed so as to protrude from the region where the through hole is formed, more than other regions. 3. A projecting part is formed, and said through hole is formed in said projecting part.
Or the blood separation device according to 3 or 4 or 5. 7. A groove is provided in an annular shape on an outer periphery of a through hole on a lower surface side of the upper main body portion on a side where a separation unit is disposed. Or the blood separation device according to 6. 8. The blood separation device according to claim 7, wherein a communication hole is provided for communicating the groove with the outer surface on the upper surface side of the upper body. 9. The blood separation device according to claim 7, wherein the separation portion has a circular sheet shape, and the diameter of the separation portion is larger than the diameter of the groove. 10. A gap is formed between a surface on the lower surface side of the upper main body, which is the side where the separation unit is disposed, and a separation portion of the separation unit. Or 3 or 4 or 5 or 6 or 7 or 8
Or the blood separation device according to 9. 11. A through hole is formed in the lower main body at a position below the suction section in a state where the suction unit is held by the lower main body. The blood separation device according to 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10. 12. The first holding portion has a plate shape, and
The said 2nd holding | maintenance part is exhibiting plate shape, The 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 characterized by the above-mentioned.
Or the blood separation device according to 10 or 11. 13. The separation unit, wherein the first holding unit has an opening, and the separation unit is held at the position of the opening, and the lower surface of the separation unit on the suction unit side of the separation unit. Is formed inside the lower surface of the first holding portion, and a concave portion is formed in an opening of the lower surface of the separation unit.
Or the blood separation device according to 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12. 14. The suction unit according to claim 14, wherein
The opening is provided in the holding part, and the suction part is provided on the second holding part so as to close the opening. Or 7
Or the blood separation device according to 8 or 9 or 10 or 11 or 12 or 13. 15. The separation unit, wherein the first holding unit has an opening, and the separation unit is held at the position of the opening, and the lower surface of the separation unit on the suction unit side of the separation unit. Is formed inside the lower surface of the first holding unit, a concave portion is formed in the opening of the lower surface of the separation unit, and in the suction unit, the opening is provided in the second holding unit. The suction unit is provided on the second holding unit so as to close the opening, and when the separation unit and the suction unit are stacked, the suction unit is located in a recess formed in the separation unit. It is fitted, and it is fitted. 1 or 2 or 3 or 4 or 5 or 6 characterized by the above-mentioned.
Or the blood separation device according to 7 or 8 or 9 or 10 or 11 or 12. 16. A fixing means for fixing the suction unit and a lower main body part of the main body part, wherein the fixing means is provided. 9 or 10 or 11 or 12 or 1
The blood separation device according to 3 or 14 or 15. 17. The switching member has a sheet shape, and a part of the switching member is disposed so as to be exposed from the main body, and the switching member is moved so that a through hole in the upper main body is formed. Characterized in that the lower end can be in an open state in which the lower end is open. The blood separation device as described in the above. 18. The blood separation device according to claim 17, wherein the switching member is provided with an opening through which the blood stored in the through hole passes. 19. The separation unit of the separation unit and the suction unit of the suction unit are in close contact with each other when the separation unit and the suction unit are stacked and held by the main body. Item 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 or 16 or 1
19. The blood separation device according to 7 or 18. 20. A blood separation apparatus for separating a non-blood cell component, which is a component other than a blood cell component, which is a plasma component or a serum component, from blood, wherein the non-blood cell component is separated. And a suction unit for adsorbing non-blood cell components separated by the separation unit; and a through-hole for dropping blood, wherein the separation unit is located below the through-hole. A switching member provided between the main body portion and the separation portion, wherein the switching member is configured to close a lower end of the through hole in the main body portion to an open state in which the lower end of the through hole is opened. A blood separation device, comprising: a switching member for switching. 21. A method according to claim 21, wherein the separation section is formed of a sheet-like separation membrane having a plurality of pores therein, the separation membrane having a structure in which the diameter of the pores gradually changes in the thickness direction. The blood separation device according to claim 20, wherein 22. The method according to claim 1, wherein a substantially constant amount of non-blood cell components is adsorbed to the adsorbing section by dropping a predetermined amount or more of blood into the separating section. The blood cell separation device according to 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15 or 16 or 17 or 18 or 19 or 20 or 21. 23. The method according to claim 1, wherein the separation unit has a capability of separating a non-blood cell component in an amount exceeding a water retention capacity of the adsorption unit when a predetermined amount or more of blood is dropped. 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 or 10 or 11 or 12 or 13 or 14 or 15
Or the blood cell separation device according to 16 or 17 or 18 or 19 or 20 or 21 or 22. 24. A blood test method for performing a blood test, wherein the blood test method comprises the steps of: (1), (2), (3), (4), (5), (6), (7) and (8);
Or 9 or 10 or 11 or 12 or 13 or 14 or 15 or 16 or 17 or 18 or 19 or 20 or 2
A sending step of sending the blood separation device according to 1 or 22 or 23 to a patient; and at least an adsorption unit in the blood separation device,
By using the blood separation device, a receiving step of receiving the non-blood cell component, which is a component other than the blood cell component, and the non-blood cell component, which is a plasma component or a serum component, is adsorbed; A blood test method, comprising: a test step of performing a test using a suctioned portion that has been suctioned. 25. The test step, wherein an extract is obtained by shaking and extracting an adsorbing portion to which a non-blood cell component is adsorbed to obtain an extract, and a predetermined measurement is performed using the obtained extract. The blood test method according to claim 24, further comprising a measuring step. 26. A blood test method for testing blood, which is a non-blood cell component that is a component other than a blood cell component obtained by separating a blood cell component from blood, and is a plasma component or a serum component. An extract producing step of obtaining an extract by shaking the adsorbent to which the non-blood cell component is adsorbed, and a measuring step of performing a predetermined measurement using the obtained extract, Blood test method to do. 27. The method according to claim 25, wherein the predetermined measurement is a measurement performed by a colorimetric method.
The blood test method according to 1. 28. The adsorbing section or adsorbent on which the non-blood cell component is adsorbed is a sheet-like separation membrane having a plurality of pores therein, and has a structure in which the diameter of the pores gradually changes in the thickness direction. 28. The blood test method according to claim 24, wherein the separated non-blood cell component is adsorbed by dropping blood onto the separation membrane.