JPS6139040B2 - - Google Patents
Info
- Publication number
- JPS6139040B2 JPS6139040B2 JP2980378A JP2980378A JPS6139040B2 JP S6139040 B2 JPS6139040 B2 JP S6139040B2 JP 2980378 A JP2980378 A JP 2980378A JP 2980378 A JP2980378 A JP 2980378A JP S6139040 B2 JPS6139040 B2 JP S6139040B2
- Authority
- JP
- Japan
- Prior art keywords
- cholesterol
- serum
- color development
- oxidizing
- free cholesterol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 21
- 235000012000 cholesterol Nutrition 0.000 claims description 10
- 210000002966 serum Anatomy 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 238000011161 development Methods 0.000 claims description 5
- 150000001447 alkali salts Chemical class 0.000 claims description 4
- 108090001060 Lipase Proteins 0.000 claims description 3
- 102000004882 Lipase Human genes 0.000 claims description 3
- 239000004367 Lipase Substances 0.000 claims description 3
- 108010055297 Sterol Esterase Proteins 0.000 claims description 3
- 102000000019 Sterol Esterase Human genes 0.000 claims description 3
- 235000019421 lipase Nutrition 0.000 claims description 3
- 108010089254 Cholesterol oxidase Proteins 0.000 claims description 2
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 claims 2
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 claims 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims 2
- 230000001590 oxidative effect Effects 0.000 claims 2
- 102000003992 Peroxidases Human genes 0.000 claims 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims 1
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 claims 1
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 claims 1
- 108040007629 peroxidase activity proteins Proteins 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000011002 quantification Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- QRLVDLBMBULFAL-UHFFFAOYSA-N Digitonin Natural products CC1CCC2(OC1)OC3C(O)C4C5CCC6CC(OC7OC(CO)C(OC8OC(CO)C(O)C(OC9OCC(O)C(O)C9OC%10OC(CO)C(O)C(OC%11OC(CO)C(O)C(O)C%11O)C%10O)C8O)C(O)C7O)C(O)CC6(C)C5CCC4(C)C3C2C QRLVDLBMBULFAL-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- UVYVLBIGDKGWPX-KUAJCENISA-N digitonin Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)C[C@@H](O)[C@H](O[C@H]5[C@@H]([C@@H](O)[C@@H](O[C@H]6[C@@H]([C@@H](O[C@H]7[C@@H]([C@@H](O)[C@H](O)CO7)O)[C@H](O)[C@@H](CO)O6)O[C@H]6[C@@H]([C@@H](O[C@H]7[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O7)O)[C@@H](O)[C@@H](CO)O6)O)[C@@H](CO)O5)O)C[C@@H]4CC[C@H]3[C@@H]2[C@@H]1O)C)[C@@H]1C)[C@]11CC[C@@H](C)CO1 UVYVLBIGDKGWPX-KUAJCENISA-N 0.000 description 1
- UVYVLBIGDKGWPX-UHFFFAOYSA-N digitonine Natural products CC1C(C2(CCC3C4(C)CC(O)C(OC5C(C(O)C(OC6C(C(OC7C(C(O)C(O)CO7)O)C(O)C(CO)O6)OC6C(C(OC7C(C(O)C(O)C(CO)O7)O)C(O)C(CO)O6)O)C(CO)O5)O)CC4CCC3C2C2O)C)C2OC11CCC(C)CO1 UVYVLBIGDKGWPX-UHFFFAOYSA-N 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2980378A JPS54123095A (en) | 1978-03-17 | 1978-03-17 | Method and reagent for measuring quantity of cholesterol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2980378A JPS54123095A (en) | 1978-03-17 | 1978-03-17 | Method and reagent for measuring quantity of cholesterol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54123095A JPS54123095A (en) | 1979-09-25 |
| JPS6139040B2 true JPS6139040B2 (ru) | 1986-09-02 |
Family
ID=12286165
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2980378A Granted JPS54123095A (en) | 1978-03-17 | 1978-03-17 | Method and reagent for measuring quantity of cholesterol |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS54123095A (ru) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0464327U (ru) * | 1990-10-16 | 1992-06-02 |
-
1978
- 1978-03-17 JP JP2980378A patent/JPS54123095A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0464327U (ru) * | 1990-10-16 | 1992-06-02 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS54123095A (en) | 1979-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lie et al. | Cholesterol oxidase-based determination, by continuous-flow analysis, of total and free cholesterol in serum. | |
| Barham et al. | An improved colour reagent for the determination of blood glucose by the oxidase system | |
| Kageyama | A direct colorimetric determination of uric acid in serum and urine with uricase-catalase system | |
| Toshihide et al. | A new colorimetric method for the determination of plasma lecithin-cholesterol acyltransferase activity | |
| Laker et al. | Spectrophotometric determination of urinary oxalate with oxalate oxidase prepared from moss. | |
| Domagk et al. | A colorimetric method using uricase and peroxidase for the determination of uric acid | |
| Magnusson et al. | Mechanisms of thyroid peroxidase-and lactoperoxidase-catalyzed reactions involving iodide. | |
| US4212938A (en) | Reagent and method for the determination of cholesterol | |
| Deacon et al. | Enzymic assay of total cholesterol involving chemical or enzymic hydrolysis--a comparison of methods. | |
| CA1125151A (en) | Triglycerides assay and reagents therefor | |
| JPS5886083A (ja) | グリセロ−ル−３−リン酸オキシダ−ゼの安定化剤 | |
| Noma et al. | Polarographic method for rapid microdetermination of cholesterol with cholesterol esterase and cholesterol oxidase. | |
| US5288606A (en) | Reagent for specific determination of fructosamine | |
| Kasidas et al. | A new enzymatic method for the determination of glycollate in urine and plasma | |
| JPS60259185A (ja) | グリセリンオキシダ−ゼの取得法 | |
| JPS5850719B2 (ja) | トリグリセライドの定量方法 | |
| Kohlbecker et al. | Direct spectrophotometric determination of serum and urinary oxalate with oxalate oxidase | |
| DK151974B (da) | Fremgangsmaade og reagens til brug ved denne, til bestemmelse af glutamat-oxalacetat-transaminase og glutamat-pyruvat-transaminase | |
| US4038146A (en) | Method of determination of serum triglycerides and reagents | |
| EP0463171B1 (en) | Method of determining fructosamines | |
| JPS6139040B2 (ru) | ||
| WO1982002212A1 (en) | Method for assaying cholinesterase activity | |
| JPH0650299B2 (ja) | サリチレートの検出方法 | |
| JPH07155196A (ja) | 生体成分の測定法 | |
| JPH0698031B2 (ja) | Ｌｄ−１アイソザイムの測定法および試薬 |