JPS6129734A - Processor for sample penetration - Google Patents
Processor for sample penetrationInfo
- Publication number
- JPS6129734A JPS6129734A JP15185584A JP15185584A JPS6129734A JP S6129734 A JPS6129734 A JP S6129734A JP 15185584 A JP15185584 A JP 15185584A JP 15185584 A JP15185584 A JP 15185584A JP S6129734 A JPS6129734 A JP S6129734A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- chemical
- pump
- chemical solution
- sample chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000035515 penetration Effects 0.000 title description 3
- 239000000126 substance Substances 0.000 claims abstract description 88
- 239000007788 liquid Substances 0.000 claims description 31
- 238000011282 treatment Methods 0.000 claims description 21
- 230000008595 infiltration Effects 0.000 claims description 14
- 238000001764 infiltration Methods 0.000 claims description 14
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005470 impregnation Methods 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 93
- 239000000243 solution Substances 0.000 description 47
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 239000012472 biological sample Substances 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000004809 Teflon Substances 0.000 description 4
- 229920006362 Teflon® Polymers 0.000 description 4
- 239000000057 synthetic resin Substances 0.000 description 4
- 229920003002 synthetic resin Polymers 0.000 description 4
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 3
- 239000003822 epoxy resin Substances 0.000 description 3
- 229920000647 polyepoxide Polymers 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 238000004891 communication Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 239000004850 liquid epoxy resins (LERs) Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011328 necessary treatment Methods 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、生物試料のごとき水分を含んだ試料を電子顕
微鏡用試料として作成してゆく課程において、上記試料
に脱水や浸透処理を行なうための装置に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention is a method for dehydrating and permeating a sample containing water, such as a biological sample, in the process of preparing the sample as a sample for an electron microscope. Regarding the device.
一般に、生物試料のごとき水分を含んだ試料を電子顕微
鏡用試料として作成するには、この試料に対し合計１３
〜２０工程にも及ぶ固定、脱水および浸透処理が施され
る。Generally, in order to prepare a sample containing water such as a biological sample as an electron microscope sample, a total of 13
~20 steps of fixation, dehydration, and infiltration are performed.
その−例を示すと、生物試料はまずアルデヒド固定（Ａ
ｌｄｅｈｙｄｅ ｆｉｘａｔｉｖｅ）やオスミウム固定
（Ｏｓｍｉｕｍ ｆｉｘａｔｉｖｅ）を施されたのち、
水洗いされてから、各種の濃度を有するエチルアルコー
ル用液に浸漬されて試料内の水分をエチルアルコールに
置換され、その後このようにして脱水処理を施された試
料はプロピレンオキサイド （Ｐｒｏｐ２１ｅｎｅ ０
ｘｉｄｅ）液に浸漬されて上記試料内のエチルアルコー
ルをプロピレンオキサイドに置換される。To give an example, biological samples are first fixed with aldehyde (A
After being treated with ldehyde fixative or osmium fixative,
After being washed with water, the sample is immersed in ethyl alcohol solutions with various concentrations to replace the moisture in the sample with ethyl alcohol.
xide) solution to replace the ethyl alcohol in the sample with propylene oxide.
さらに、この試料はプロピレンとエポキシ樹脂のごとき
合成樹脂との混合液に浸漬されて浸透処理を施されたの
ち、最終的には液状の合成樹脂としてのエポキシ樹脂内
に浸漬されて、試料内の水分がすべてエポキシ樹脂に置
換されるようになっている。Furthermore, this sample is immersed in a mixed solution of propylene and a synthetic resin such as epoxy resin for penetration treatment, and then finally immersed in epoxy resin, which is a liquid synthetic resin. All water is replaced with epoxy resin.
なお、上述の固定、脱水および浸透処理は、各工程ごと
に試料を浸漬する時間や温度等が設定されている。Note that in the above-mentioned fixation, dehydration, and infiltration treatments, the time and temperature at which the sample is immersed are set for each step.
このようにして浸透処理等を施された生物試料は、その
内部に水分を含まないため、長時間の保存に耐え、しか
も電ｆ−顕微鏡用試料として最適のものになる。Since the biological sample subjected to the permeation treatment in this manner does not contain any water, it can withstand long-term storage and becomes optimal as a sample for electron f-microscope.
以上のような試料の浸透処理手段としては、従来、第７
図に示すようなものがある （特公昭５７−１９３７２
号公報参照）。Conventionally, as a means for infiltration treatment of samples as described above, the seventh method has been used.
There is something like the one shown in the figure (Tokuko Sho 57-19372
(see publication).
すなわち、この浸透処理手段にあっては、透明ガラス等
の透明材から成り同一形状を有する複数の円筒状薬液槽
ｌが、薬液レベルｌａよりもＪ一方で、透明ガラス等の
透明材から成る逆Ｕ字竹２を介して、互いに連結されて
いる。That is, in this infiltration treatment means, a plurality of cylindrical chemical liquid tanks l made of a transparent material such as transparent glass and having the same shape are lower than the chemical liquid level la. They are connected to each other via a U-shaped bamboo 2.
また、各薬液槽１内には、この薬液槽１との間の薬液の
連通を許容しうる連通口としての孔を多数個有する多孔
材から成る試料案内路としてのＵ字管１ｂが配設されて
おり、これにより生物試料等の試料を薬液槽ｌ内におけ
るエチルアルコールやプロピレンオキサイドや液状のエ
ポキシ樹脂等のごとき薬液に浸漬させながら、該試料を
案内できるようになっている。Further, in each chemical liquid tank 1, a U-shaped tube 1b is provided as a sample guide path made of a porous material having a large number of holes as communication ports that allow communication of the chemical liquid with the chemical liquid tank 1. This makes it possible to guide a sample such as a biological sample while immersing it in a chemical solution such as ethyl alcohol, propylene oxide, or liquid epoxy resin in the chemical solution tank l.
さらに、多孔材から成るＵ字管１ｂの上端は逆Ｕ字管２
に接続されている。Furthermore, the upper end of the U-shaped tube 1b made of a porous material is connected to an inverted U-shaped tube 2.
It is connected to the.
そして、この装置には、試料を各０字管１ｂおよび逆Ｕ
字管２に沿い駆動して順次、固定、脱水、および浸透を
行なわせるための試料搬送手段が設けられている。In this device, the sample is placed in each O-shaped tube 1b and inverted U-shaped tube 1b.
A sample transport means is provided for driving along the tube 2 to sequentially fix, dehydrate, and infiltrate.
この試料搬送手段は、チェーンやワイヤロープのごとき
可撓性条体４をそなえており、この条体４は薬液の流通
を許容し、その内部に試料を収納した容器３に連結され
て、各薬液槽ｌのＵ字管ｔｂおよび逆Ｕ字管２を通過す
べく、無端状に配設されている。This sample transport means is equipped with a flexible strip 4 such as a chain or wire rope, and this strip 4 allows the flow of a chemical solution and is connected to a container 3 containing a sample inside. It is disposed in an endless manner so as to pass through the U-shaped tube tb of the chemical solution tank l and the inverted U-shaped tube 2.
さらに、この条体４はスプロケットホイールまたはプー
リのごとき動力伝達輪５を介して駆動機構の主要部をな
す電動機６により駆動せしめられ、これにより容器３を
移動させることができるようになっている。Furthermore, this strip 4 is driven by an electric motor 6, which is the main part of a drive mechanism, via a power transmission wheel 5 such as a sprocket wheel or a pulley, thereby making it possible to move the container 3.
そして、この浸透処理装置は、電動Ｉｔ６を駆動させる
ことにより、条体４に連結された容器３を多孔材製Ｕ字
管ｌｂ内で移動させ、次々と薬液槽内に浸漬させること
により、容器３内に収納された試料を脱水、浸透処理す
るものである。This infiltration treatment apparatus moves the containers 3 connected to the strips 4 within the U-shaped tube lb made of porous material by driving the electric It6, and immerses the containers one after another in the chemical tank. This is to dehydrate and infiltrate the sample stored in the chamber.
しかしながら、以上のような浸透処理装置にあっては、
試料を１個づつ容器に・収納して処理する必要があるた
め、一度に処理できる試ネ４の数は少なく、その結果、
処理後における試料の均一性が得られないという不具合
がある。また、試料を１個づつ容器に収納する必要があ
るため、処理装置には必ず作業者が付いて処理された試
料を取り出し、次に処理すべき試料を容器に収納しなけ
ればならず、その作業が煩わしいものであった。However, in the above-mentioned infiltration treatment equipment,
Because it is necessary to store and process each sample one by one in a container, the number of samples that can be processed at one time is small, and as a result,
There is a problem that uniformity of the sample cannot be obtained after processing. In addition, since it is necessary to store samples one by one in a container, a worker must be present at the processing equipment to take out the processed sample, then store the next sample to be processed in the container. The work was cumbersome.
また、」二記浸透処理装置は薬液槽内に試料を浸漬する
ものであるから薬液層は常に多量の薬液で満たされてい
る必要があり、薬液の交換作業を頻繁に行なわなければ
ならないという問題があった。更に、薬液槽内を、試料
を収納した容器が移動すると、薬液から気泡が発生し易
いために良好な浸透処理ができない場合がある。In addition, since the infiltration treatment apparatus described in Section 2 immerses the sample in a chemical solution tank, the chemical layer must always be filled with a large amount of chemical solution, resulting in the problem of having to frequently replace the chemical solution. was there. Furthermore, if a container containing a sample moves within the chemical solution tank, bubbles are likely to be generated from the chemical solution, making it impossible to perform a good infiltration treatment.
本発明は、上記事情に鑑みてなされたものであり、その
手段は、試料の脱水、浸透処理をするための薬液を入れ
るべき複数の薬液槽と、処理すべき複数の試料を収納可
能な試料収納部と、上記薬液を上記各薬液槽と試料収納
部との間で流動させるためのポンプとを、切換弁を介し
て配管で相互に連通せしめると共に、上記試料収納部に
送られた薬液中の気体を除去するために真空ポンプを設
けて試料収納部と連通せしめたという構成によってなさ
れる。The present invention has been made in view of the above circumstances, and its means include a plurality of chemical liquid tanks into which chemical liquids are to be placed for dehydration and osmosis treatment of samples, and a sample capable of storing a plurality of samples to be treated. The storage section and a pump for flowing the chemical solution between each of the chemical solution tanks and the sample storage section are communicated with each other via piping via a switching valve, and the chemical solution sent to the sample storage section is In order to remove the gas, a vacuum pump is provided and communicated with the sample storage section.
以下、添付図面に基づいて本発明の詳細な説明する。 Hereinafter, the present invention will be described in detail based on the accompanying drawings.
第１図は本発明の実施例を示すものであるにの試料浸透
処理装置（以下、処理装置）は、試料の脱水、浸透処理
をするための薬液７ （第１図中７ａ、７ｂで示す）を
入れた複数個の薬液槽８ （第１図中８ａ、８ｂで示す
）と、処理すべき試料８を入れた試料収納部１０と、薬
液７を吸入、排出し薬液を流動させるポンプ、例え−ば
シリンダーポンプ１１とが、薬液の流路を変更させる切
換弁１２を介して配管１３によって相互に連結されてい
る。FIG. 1 shows an embodiment of the present invention. A sample permeation treatment apparatus (hereinafter referred to as the treatment apparatus) includes a chemical solution 7 (indicated by 7a and 7b in FIG. 1) for dehydrating and permeating a sample. ) containing a plurality of chemical liquid tanks 8 (indicated by 8a and 8b in FIG. 1), a sample storage section 10 containing a sample 8 to be processed, a pump that sucks in and discharges the chemical liquid 7 and causes the chemical liquid to flow; For example, a cylinder pump 11 is interconnected by a pipe 13 via a switching valve 12 that changes the flow path of the chemical liquid.
上記薬液槽８には、例えばエチルアルコールやプロピレ
ンオキサイドのように試料８の脱水、浸透処理に用いる
べき薬液が入れられている。The chemical liquid tank 8 contains a chemical liquid to be used for dehydration and permeation treatment of the sample 8, such as ethyl alcohol or propylene oxide.
次に、上記試料収納部１０は、試料９を入れるべき多数
の収納孔１４を有する断面矩形状の試料ホルダー１５と
、該試料ホルダー１５を収納する箱状の試料室１６とを
備えるものである。Next, the sample storage section 10 includes a sample holder 15 having a rectangular cross section and having a large number of storage holes 14 into which the samples 9 are to be placed, and a box-shaped sample chamber 16 that accommodates the sample holder 15. .
試料ホルダー１５は、非接着性の材質、例えばテフロン
等で形成されており、第２図およびｉ３図に示すように
、上記試料孔１４の上端傾斜部には試料を識別するため
の番号等の符号１７が凸状又は凹状に施され、最終的に
合成樹脂を流し込んで試料を固めたときに、符号１７が
樹脂に型どられ、試料８を識別できるようになっている
。また、試料ホルダー１５の上面には薬液７が流入した
ときに試料８が試料孔１４から飛び出さないようにメツ
シュ１８が載置され、他方、試料ホルダー１５の下面に
は試料の脱落を防止するためのメツシュ１８が張られて
いる。この試料ホルダー１５は、処理すべき試料の数に
応じて第４図ないし第６図に示すような、各種の大きさ
のものを用いることが可能である。そして、この試料ホ
ルダー１５は、その外周を覆うホルダーケース−２０に
収められて、試料室１６に収納される。尚、試料室１６
内には、薬液７が勢いよく試料室１６内に流入するのを
防止し薬液が均等に拡散するように整流板２１が設けら
れている。また、試料室１６はその上部が着脱自在の上
Ｍ２２によって覆われており、該上蓋２２および導管２
３を介して真空ポンプ２４と連通している。The sample holder 15 is made of a non-adhesive material, such as Teflon, and as shown in FIGS. The code 17 is provided in a convex or concave shape, and when the synthetic resin is finally poured and the sample is hardened, the code 17 is molded into the resin so that the sample 8 can be identified. Further, a mesh 18 is placed on the top surface of the sample holder 15 to prevent the sample 8 from jumping out from the sample hole 14 when the chemical solution 7 flows in, and on the other hand, a mesh 18 is placed on the bottom surface of the sample holder 15 to prevent the sample from falling out. There is a mesh 18 for this purpose. The sample holder 15 can be of various sizes as shown in FIGS. 4 to 6 depending on the number of samples to be processed. The sample holder 15 is housed in a holder case 20 that covers the outer periphery of the sample holder 15, and is housed in the sample chamber 16. In addition, sample chamber 16
A rectifying plate 21 is provided inside to prevent the chemical solution 7 from flowing forcefully into the sample chamber 16 and to spread the chemical solution evenly. Further, the upper part of the sample chamber 16 is covered by a removable upper M22, and the upper lid 22 and the conduit 2
It communicates with a vacuum pump 24 via 3.
ところで、シリンダーポンプ１１および配管１３内の薬
液の流路を切換える切換弁１２は制御装置２５によって
制御される。すなわち、制御装置２５は、シリンダーポ
ンプ１１および切換弁１２を制御して使用すべき薬液７
の順番と使用量および夫々の薬液ごとに予め設定されて
いる処理時間等を調節し浸透処理を進行させるものであ
る。また制御装置２５は、真空ポンプ２４の圧力制御お
よびリークさせるときの開放弁２６の制御も行なうもの
である。そして、かかる制御の状態は制御装置２５に接
続された表示装置２７に表示され、使用中の薬液の種類
、その設定処理時間および経過時間、その他処理工程の
把握に必要な情報（例えば室温等）を知ることができる
ようになっている。By the way, the cylinder pump 11 and the switching valve 12 that switches the flow path of the chemical liquid in the piping 13 are controlled by the control device 25. That is, the control device 25 controls the cylinder pump 11 and the switching valve 12 to control the chemical liquid 7 to be used.
The infiltration treatment is progressed by adjusting the order and amount used, and the treatment time set in advance for each chemical solution. The control device 25 also controls the pressure of the vacuum pump 24 and the release valve 26 when leaking. The state of this control is displayed on the display device 27 connected to the control device 25, and information such as the type of chemical solution in use, its set processing time and elapsed time, and other information necessary to understand the processing process (for example, room temperature, etc.) It is now possible to know.
以上のように構成された処理装置の作用を説明する。The operation of the processing device configured as above will be explained.
処理工程の最初に１例えば薬液７ａが使用されるものと
すると、制御装置２５からの信号に基づいて、切換弁１
２がシリンダーポンプ１１と薬液槽８ａを開状態として
連通させ、他への流路を閉状態とする。次に、制御装置
２５からの信号に基づいてシリンダーポンプ１１が作動
し、薬液７ａを吸引する。この時、吸引される薬液７ａ
の量は、試料ホルダー１５の大きさ乃至処理すべき試料
の数によって予め制御装置に設定されている。そして、
薬液７ａがシリンダーポンプ１１に吸引されると、切換
弁１２がシリンダーポンプ１．１と試料室１Ｂとを開状
態として連通させ、他への流路を閉状態とする。この状
態でシリンダーポンプ１１が薬液７ａを排出することに
より、薬液７ａが試料室１６へ送られることになる。尚
、試料室１θに送られた薬液７ａは試料ホルダー１５お
よびメツシュ１８の下（１１に設けられた整流板２１に
よって試料室１６の全体に平均的に拡散し、試料９を均
一に浸透させるようになっている。また、薬液７ａは試
料９を浸透し、更に試料ホルダー１５の収納孔１４の上
部に達するまで試料室１６を満たすようになっている。If, for example, the chemical solution 7a is used at the beginning of the treatment process, the switching valve 1 is activated based on a signal from the control device 25.
2 opens the cylinder pump 11 and the chemical tank 8a to communicate with each other, and closes the flow path to other parts. Next, the cylinder pump 11 is activated based on a signal from the control device 25 to suck the chemical solution 7a. At this time, the medicinal liquid 7a to be aspirated
The amount is set in advance in the control device depending on the size of the sample holder 15 or the number of samples to be processed. and,
When the chemical solution 7a is sucked into the cylinder pump 11, the switching valve 12 opens the cylinder pump 1.1 and the sample chamber 1B to communicate with each other, and closes the flow path to the other parts. When the cylinder pump 11 discharges the chemical solution 7a in this state, the chemical solution 7a is sent to the sample chamber 16. The chemical solution 7a sent to the sample chamber 1θ is spread evenly throughout the sample chamber 16 by a rectifying plate 21 provided under the sample holder 15 and the mesh 18 (11), so that the sample 9 is uniformly penetrated. Further, the chemical liquid 7a permeates the sample 9 and further fills the sample chamber 16 until it reaches the upper part of the storage hole 14 of the sample holder 15.
尚、薬液が流動する場合には、従来の処理装置のように
薬液内を試料が移動する場合に比べて薬液に気泡が生ず
ることは少ないが、この処理装置においては、真空ポン
プ２４を設は薬液？ａを十分送った後、それまで開放し
ていた開放弁２６を閉じ、真空ポンプ２４を作動させる
ことによって、薬液７ａ内の空気やガスを吸引するよう
になっている。また、このときシリンダーポンプ１１を
微動させて試料室１６内の薬液を動かすと薬液中の空気
やガスが完全に取り除かれ、浸透効果が増加する。Note that when the chemical solution flows, bubbles are less likely to be generated in the chemical solution compared to when the sample moves within the chemical solution as in conventional processing equipment, but in this processing equipment, the vacuum pump 24 is not installed. Liquid medicine? After sufficiently supplying the chemical solution 7a, the open valve 26, which had been open until then, is closed and the vacuum pump 24 is operated, thereby sucking the air or gas in the chemical solution 7a. Further, at this time, if the cylinder pump 11 is slightly moved to move the chemical liquid in the sample chamber 16, air and gas in the chemical liquid are completely removed, increasing the permeation effect.
一方、浸漬する必要な時間が経過すると、開放弁２６が
開くと共にシリンダーポンプ１１が作動して、試料室１
８内の薬液７ａを吸入する。On the other hand, when the necessary time for immersion has elapsed, the release valve 26 opens and the cylinder pump 11 operates, and the sample chamber 1
Inhale the drug solution 7a in 8.
そして、切換弁１２がシリンダーポンプ１１と薬液槽８
ａを開通させ他の流路を閉じた後、シリンダーポンプ１
１が薬液７ａを排出することにより、薬液７ａは薬液槽
８ａに戻される。The switching valve 12 is connected to the cylinder pump 11 and the chemical tank 8.
After opening a and closing the other channels, cylinder pump 1
1 discharges the chemical liquid 7a, thereby the chemical liquid 7a is returned to the chemical liquid tank 8a.
続いて、薬液７ｂが使用される番となり、切換弁１２が
薬液槽８ｂとシリンダーポンプ１１とを開通し、他の流
路を閉じると、シリンダーポンプ１１が薬液８ｂを吸入
し、それ以降、以上と同様の処理工程が繰り返されるこ
とにより必要とされる処理工程が終了する。Next, it is the turn of the chemical solution 7b to be used, and when the switching valve 12 opens the chemical solution tank 8b and the cylinder pump 11 and closes the other channels, the cylinder pump 11 sucks the chemical solution 8b, and from then on, the above The necessary treatment steps are completed by repeating the same treatment steps.
尚、上記処理装置による必要な処理工程が終了した後の
処理を説明すると、まず試料室１８の上蓋２２を外し、
ホルダーケース２０を取り出す。そして上側のメツシュ
１８を外してホルダーケース２０の」二にテフロンの板
を載せる。In addition, to explain the processing after the necessary processing steps by the above-mentioned processing device are completed, first, remove the upper lid 22 of the sample chamber 18,
Take out the holder case 20. Then, remove the upper mesh 18 and place a Teflon plate on the top of the holder case 20.
その状態で、ホルダーケース２０をひつくり返すとテフ
ロン板の上に試料ホルダー１５と下側のメツシュ１９が
落ち、ホルダーケース２０とメツシュ１９を外すと試料
８の入った試料ホルダー１５がテフロン板の上に残る。In this state, when the holder case 20 is turned over, the sample holder 15 and the lower mesh 19 fall onto the Teflon plate, and when the holder case 20 and mesh 19 are removed, the sample holder 15 containing the sample 8 is placed on the Teflon plate. remain on top.
そこへ合成樹脂を流し込み固めた後、１個づつ抜きとつ
て、全処理作業が終了する。尚、試料ホルダー１５の試
料８を入れる収納孔１４の上部には、記号や番号などの
符号１７が凸状又は凹状に施されているから、処理され
た各試料を容易に識別できるようになっている。After pouring synthetic resin into it and solidifying it, the pieces are removed one by one, completing the entire process. Incidentally, a code 17 such as a symbol or number is provided in a convex or concave shape on the upper part of the storage hole 14 into which the sample 8 of the sample holder 15 is placed, so that each processed sample can be easily identified. ing.
尚、上記実施例においては、試料の収納部を試料室と試
料ホルダー等から構成されるものとして説明したが、本
発明においては、複数の試料を収納し、薬液を送り込ん
で試料の浸透処理ができるものであれば、上記実施例の
ような試料収納部である必要はない。また、薬液槽の数
は必要に応じて適宜変更することが可能である。更に、
上記実施例においては、薬液を流動させるポンプとして
、シリンダーポンプを例にとって説明したが、薬液を吸
入、排出して同様の作用をするものであればシリンダー
ポンプに限定されることはない。In the above embodiment, the sample storage section was explained as being composed of a sample chamber, a sample holder, etc., but in the present invention, a plurality of samples are stored, and a chemical solution is sent to perform the penetration treatment of the samples. If it is possible, it does not need to be a sample storage part like the one in the above embodiment. Further, the number of chemical liquid tanks can be changed as necessary. Furthermore,
In the above embodiments, a cylinder pump has been described as an example of a pump for flowing a medicinal solution, but the pump is not limited to a cylinder pump as long as it has the same effect by sucking in and discharging a medicinal solution.
以」−説明したように、本発明に係る試料浸透処理装置
によれば、多数の試料を同時にかつ自動的に処理できる
ので、各試料を均一に処理できる。また、処理作業中に
作業者が装置に付いている必要はなくなり、従来の装置
における試料の取り外し、収納の煩わしさがなくなった
。As described above, according to the sample infiltration processing apparatus according to the present invention, a large number of samples can be processed simultaneously and automatically, so that each sample can be processed uniformly. Furthermore, there is no longer a need for an operator to be present with the apparatus during processing operations, eliminating the trouble of removing and storing samples in conventional apparatuses.
また、本発明の試料浸透処理装置は試料を移動させるも
のではなく、薬液を移動させるものであるから、薬液に
気泡を生ずることが少なくなり、しかも真空ポンプによ
って、薬液中の空気やガス等の気体を完全に取り除くよ
うにしたから、試料の細部にまで十分薬液が浸透し、良
好な処理をすることができる。In addition, since the sample infiltration treatment device of the present invention does not move the sample but moves the chemical solution, it is less likely to generate air bubbles in the chemical solution, and the vacuum pump can remove air, gas, etc. Since the gas is completely removed, the chemical solution can sufficiently penetrate into the details of the sample, allowing for good processing.
また、薬液自体を移動させることにより、異った薬液が
相互に混って試料に浸透することも試料を良好にするこ
とに資するものである。Further, by moving the chemical liquid itself, different chemical liquids mix with each other and permeate into the sample, which also contributes to improving the quality of the sample.
しかも本発明によれば、試料収納部内の試料を浸漬する
に必要最小限の薬液を使用すれば良いから、薬液の使用
量を抑えることができ、その結果、薬液の交換回数も減
少して、交換作業の煩わしさを減らすことができる。Moreover, according to the present invention, it is sufficient to use the minimum amount of chemical solution necessary to immerse the sample in the sample storage section, so the amount of chemical solution used can be reduced, and as a result, the number of times the chemical solution is replaced is also reduced. The hassle of replacement work can be reduced.
また、試料収納部内に収納する試料の数が少ないときに
は薬液の使用量を少量とすることができるので、薬液の
節約ができるものである。Further, when the number of samples stored in the sample storage section is small, the amount of chemical solution used can be reduced, so that the amount of chemical solution can be saved.
第１図は本発明の実施例に係る試料浸透処理装置を示す
断面説明図、第２図は上記実施例に係る試料収納部の試
料ホルダーを示す断面図、第３図は上記試料ホルダーに
設けられた収納孔を示す斜視図、第４図ないし第８図は
、上記実施例において使用される各種の試料ホルダーを
示す断面図、第７図は従来の試料浸透処理装置を示す断
面図である。
８・・・薬液槽 ８・・・試料１０・・・試料
収納部 １１・・・シリンダーポンプ１２・・・切
換弁 １３・・・導管２５・・・制御部
第４図
第 ５ 図
第７図FIG. 1 is a cross-sectional explanatory diagram showing a sample infiltration treatment apparatus according to an embodiment of the present invention, FIG. 2 is a cross-sectional diagram showing a sample holder of a sample storage section according to the above embodiment, and FIG. FIG. 4 to FIG. 8 are cross-sectional views showing various sample holders used in the above embodiments, and FIG. 7 is a cross-sectional view showing a conventional sample infiltration processing device. . 8... Chemical solution tank 8... Sample 10... Sample storage section 11... Cylinder pump 12... Switching valve 13... Conduit 25... Control section Fig. 4 Fig. 5 Fig. 7
Claims (1)
数の薬液槽と、処理すべき複数の試料を収納可能な試料
収納部と、上記薬液を上記各薬液槽と試料収納部との間
で流動させるためのポンプとを、切換弁を介して配管で
相互に連通せしめると共に、上記試料収納部に送られた
薬液中の気体を除去するために真空ポンプを設けて試料
収納部と連通せしめたことを特徴とする試料浸透処理装
置。A plurality of chemical liquid tanks into which chemical liquids for dehydration and permeation treatment of samples are to be placed, a sample storage unit capable of storing a plurality of samples to be processed, and a plurality of chemical liquid tanks to be stored between each of the chemical liquid tanks and the sample storage unit. A pump for causing the chemical solution to flow was connected to each other via piping via a switching valve, and a vacuum pump was provided to remove gas in the chemical solution sent to the sample storage section and communicated with the sample storage section. A sample infiltration processing device characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15185584A JPS6129734A (en) | 1984-07-21 | 1984-07-21 | Processor for sample penetration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15185584A JPS6129734A (en) | 1984-07-21 | 1984-07-21 | Processor for sample penetration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6129734A true JPS6129734A (en) | 1986-02-10 |
| JPH0367217B2 JPH0367217B2 (en) | 1991-10-22 |
Family
ID=15527731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15185584A Granted JPS6129734A (en) | 1984-07-21 | 1984-07-21 | Processor for sample penetration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6129734A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63171337A (en) * | 1986-07-24 | 1988-07-15 | スペルコ インコ−ポレイテツド | Vacuum manifold for extraction treatment |
| JPH0221238A (en) * | 1988-07-08 | 1990-01-24 | Power Reactor & Nuclear Fuel Dev Corp | Resin injector for preparing metal phase sample |
| WO2002075280A2 (en) * | 2001-03-16 | 2002-09-26 | Memorec Bi0Tec Gmbh Medizinisch-Molekulare Entwicklung, Köln | Examination device and method for examination of chemical and/or biological samples |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5670440A (en) * | 1979-11-14 | 1981-06-12 | Chiyoda Seisakusho:Kk | Device for embedding treatment of living-body organization |
| JPS56150325A (en) * | 1980-04-22 | 1981-11-20 | Yukitaka Ajimi | Vaccum device |
| JPS57103031A (en) * | 1981-10-30 | 1982-06-26 | Akashi Seisakusho Co Ltd | Sample osmosis treatment device |
-
1984
- 1984-07-21 JP JP15185584A patent/JPS6129734A/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5670440A (en) * | 1979-11-14 | 1981-06-12 | Chiyoda Seisakusho:Kk | Device for embedding treatment of living-body organization |
| JPS56150325A (en) * | 1980-04-22 | 1981-11-20 | Yukitaka Ajimi | Vaccum device |
| JPS57103031A (en) * | 1981-10-30 | 1982-06-26 | Akashi Seisakusho Co Ltd | Sample osmosis treatment device |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63171337A (en) * | 1986-07-24 | 1988-07-15 | スペルコ インコ−ポレイテツド | Vacuum manifold for extraction treatment |
| JPH0221238A (en) * | 1988-07-08 | 1990-01-24 | Power Reactor & Nuclear Fuel Dev Corp | Resin injector for preparing metal phase sample |
| WO2002075280A2 (en) * | 2001-03-16 | 2002-09-26 | Memorec Bi0Tec Gmbh Medizinisch-Molekulare Entwicklung, Köln | Examination device and method for examination of chemical and/or biological samples |
| WO2002075280A3 (en) * | 2001-03-16 | 2003-05-01 | Memorec Stoffel Gmbh | Examination device and method for examination of chemical and/or biological samples |
| US7378279B2 (en) | 2001-03-16 | 2008-05-27 | Memorec Biotec Gmbh | Examination device and method for examination of chemical and/or biological samples |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0367217B2 (en) | 1991-10-22 |
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