JPH033182B2 - - Google Patents
Info
- Publication number
- JPH033182B2 JPH033182B2 JP57039637A JP3963782A JPH033182B2 JP H033182 B2 JPH033182 B2 JP H033182B2 JP 57039637 A JP57039637 A JP 57039637A JP 3963782 A JP3963782 A JP 3963782A JP H033182 B2 JPH033182 B2 JP H033182B2
- Authority
- JP
- Japan
- Prior art keywords
- cassette
- staining
- specimen
- staining solution
- specimen transport
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012192 staining solution Substances 0.000 claims description 23
- 239000008280 blood Substances 0.000 claims description 14
- 210000004369 blood Anatomy 0.000 claims description 14
- 238000004043 dyeing Methods 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 4
- 230000032258 transport Effects 0.000 description 32
- 238000010186 staining Methods 0.000 description 17
- 238000000034 method Methods 0.000 description 13
- 239000007788 liquid Substances 0.000 description 11
- 230000008569 process Effects 0.000 description 11
- 238000012856 packing Methods 0.000 description 7
- 230000007246 mechanism Effects 0.000 description 6
- 238000000386 microscopy Methods 0.000 description 5
- 238000003825 pressing Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011112 process operation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000007664 blowing Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
この発明は、血液像検査およびその前処理を含
む自動化機器における血液塗沫標本染色工程に使
用される染色装置に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a staining device used in a blood smear staining process in automated equipment including blood image testing and its pretreatment.
血液像検査は大きく分けて標本作成のための前
処理と顕微鏡による検鏡の２つの工程から成り立
つており、前処理は更に血液の塗沫工程と染色工
程に別れ、全体として血液塗沫→染色→検鏡とい
う流れを形成する。現状において、これら３工程
は独立して行なわれており、全体としての自動化
が望まれている。 Blood image testing can be broadly divided into two steps: pretreatment for specimen preparation and examination using a microscope.The pretreatment is further divided into a blood smear process and a staining process, and the overall process consists of blood smear → staining. → Forming the flow of speculum. Currently, these three steps are performed independently, and automation of the entire process is desired.
前記３工程を連続して結ぶ自動化を考えた場
合、特に染色工程の処理には、標本を順次一枚ず
つ染色するフロー式と、複数の標本を同時に液槽
にひたすバツチ処理方式が考えられる。両方式を
比較した場合、後者は前者に比べ多量標本の処理
時間、制御性、機構簡易化、コスト等の点で優れ
ているが、染色液槽がデツドスペースとなる欠点
を有している。 When considering automation that connects the three steps in succession, particularly for the staining process, two methods are considered: a flow method in which specimens are stained one by one, and a batch processing method in which multiple specimens are immersed in a liquid bath at the same time. When comparing both types, the latter is superior to the former in terms of processing time for large quantities of specimens, controllability, simplified mechanism, cost, etc., but it has the disadvantage that the staining solution tank is a dead space.
そこで、この発明は上記従来の欠点を除去する
ためになされたものであり、染色槽そのものによ
るデツドスペースを少なくし、かつ効率よく検鏡
工程に標本を移送することが可能な染色装置を提
供することを目的とする。 Therefore, the present invention was made to eliminate the above-mentioned drawbacks of the conventional method, and an object of the present invention is to provide a staining device that can reduce the dead space caused by the staining tank itself and can efficiently transfer specimens to a microscopy process. With the goal.
この発明は上記目的を達成するために、底部に
染色液排出口を備えた標本搬送用カセツトに血液
塗沫標本を収納し、その標本搬送用カセツトを、
開閉自在な排出口をもつ染色液排出路を備えその
染色液排出路の入口が表面に形成されたカセツト
載置台に、前記標本搬送用カセツトの内部と前記
染色液排出路とが連通するように載置し、さらに
載置した標本搬送用カセツトを押え板などで押圧
しその底部を封着することにより染色液槽が形成
され、染色終了後その底部の封着を解除すること
により標本搬送用カセツトが検鏡工程などに移送
可能な状態になるようにしたものである。 In order to achieve the above object, the present invention stores a blood smear specimen in a specimen transport cassette equipped with a stain solution outlet at the bottom, and transports the specimen transport cassette into a specimen transport cassette.
A cassette mounting table is provided with a staining solution discharge path having a discharge port that can be opened and closed, and an entrance of the staining solution discharge path is formed on the surface, so that the inside of the specimen transport cassette and the staining solution discharge path communicate with each other. A staining solution bath is formed by placing the placed specimen transport cassette with a presser plate or the like and sealing the bottom of the cassette.After staining is completed, the bottom of the cassette is unsealed and the specimen transport cassette is pressed. This allows the cassette to be transported to a microscopy process or the like.
そしてこの発明によれば、上述のバツチ処理方
式の染色によるメリツトを生かしつつ、デツドス
ペースを少なくし、検鏡工程へ効率よく標本を移
送できるという効果を有する。 According to the present invention, while taking advantage of the above-mentioned advantages of batch processing staining, the dead space can be reduced and specimens can be efficiently transferred to the microscopy process.
以下、この発明による一実施例を図面に基づい
て詳細に説明する。 Hereinafter, one embodiment of the present invention will be described in detail based on the drawings.
第１図はこの実施例の染色装置全体を示す斜視
図、第２図は同染色装置の構造を示す構造図であ
る。血液塗沫標本１はプレパラートの表面に血液
が自動血液塗沫ユニツトなどにより塗沫されたも
のである。標本搬送用カセツト２は、上部および
底部６が開口され、内面に垂直に刻設した溝１２
に挿入された血液塗沫標本１を植立して収納す
る。この標本搬送用カセツト２の内部は、染色液
排出部を兼ねる開口底部６が封着されることによ
り染色液槽を形成する。標本搬送用カセツト２の
ローデイングおよびアンローデイングは図示しな
いカセツト移送装置により行われる。カセツト載
置台３は、染色液槽形成のために標本搬送用カセ
ツト２が載置される台であり、その載置箇所に表
面から下方に貫通する染色液排出路１７が形成さ
れたものである。標本搬送用カセツト２は開口底
部６が染色液排出路１７の入口に対向してパツキ
ン４を介して載置される。そして、染色液排出路
１７の排出口７には自動バルブ１３が配設され、
排出口７の開閉が行われる。使用済みの染色液は
自動バルブ１３を開放することによりチユーブ１
４を通つて排液タンク１５に溜められる。また、
カセツト載置台３には、載置された標本搬送用カ
セツト２を押圧する押圧機構が取り付けられてい
る。カセツト押え板５は標本搬送用カセツト２を
パツキン４に押え付ける板であり、リンク８，９
とモータ１０とのカセツト押え板駆動機構の作用
により揺動運動し、先端部にて標本搬送用カセツ
ト２の上面両端部を押圧する。染色に必要な染色
第１液１８ａ、バツフアー液１８ｂ、染色第２液
１８ｃ，…，洗浄水18nなどはそれぞれタンク１
１ａ，１１ｂ，１１ｃ，…，１１ｎに収容されて
いる。これらの液は注入ポンプ１６ａ，１６ｂ，
１６ｃ…１６ｎの駆動により順次染色液槽すなわ
ち標本搬送用カセツト２、カセツト載置台３、パ
ツキン４により構成される槽に注入される。前記
カセツトローデイング兼アンローデイング機構、
前記カセツト押え板駆動機構、自動バルブ１３お
よび注入ポンプ１６ａ，…，１６ｎはマイクロプ
ロセツサーにより制御されている。 FIG. 1 is a perspective view showing the entire staining apparatus of this embodiment, and FIG. 2 is a structural diagram showing the structure of the same staining apparatus. Blood smear sample 1 is obtained by smearing blood onto the surface of a preparation using an automatic blood smear unit or the like. The specimen transport cassette 2 has an open top and bottom 6 and a groove 12 cut vertically into the inner surface.
The blood smear 1 inserted into the body is planted and stored. The interior of the specimen transport cassette 2 forms a staining solution tank by sealing an open bottom 6 which also serves as a staining solution discharge section. Loading and unloading of the specimen transport cassette 2 is performed by a cassette transport device (not shown). The cassette mounting table 3 is a table on which the specimen transport cassette 2 is placed in order to form a staining solution tank, and a staining solution discharge path 17 is formed at the mounting location to penetrate downward from the surface. . The specimen transport cassette 2 is placed with the opening bottom 6 facing the entrance of the staining solution discharge path 17 via the packing 4. An automatic valve 13 is disposed at the discharge port 7 of the staining solution discharge path 17.
The discharge port 7 is opened and closed. The used staining solution is removed from the tube 1 by opening the automatic valve 13.
4 and is stored in a drainage tank 15. Also,
A pressing mechanism is attached to the cassette mounting table 3 for pressing the specimen transport cassette 2 placed thereon. The cassette holding plate 5 is a plate that holds the specimen transport cassette 2 against the packing 4, and links 8, 9
It swings under the action of the cassette holding plate drive mechanism of the cassette and motor 10, and its tip presses both ends of the upper surface of the specimen transport cassette 2. The first dyeing liquid 18a, buffer liquid 18b, second dyeing liquid 18c, ..., washing water 18n, etc. necessary for dyeing are stored in tanks 1, respectively.
1a, 11b, 11c,..., 11n. These liquids are supplied to the injection pumps 16a, 16b,
16c...16n are driven to sequentially inject the staining solution into a tank consisting of a specimen transport cassette 2, a cassette mounting table 3, and a packing 4. the cassette loading and unloading mechanism;
The cassette holding plate drive mechanism, automatic valve 13 and injection pumps 16a, . . . , 16n are controlled by a microprocessor.
次に、この実施例の染色装置による染色工程動
作を第２図および第３図のフローチヤートにより
説明する。 Next, the dyeing process operation by the dyeing apparatus of this embodiment will be explained with reference to the flowcharts of FIGS. 2 and 3.
初めに、標本搬送用カセツト２がカセツト移送
装置によりパツキン４上にローデイングされる。
なお、標本搬送用カセツト２においては、すでに
自動血液塗沫ユニツトで血液が塗沫された標本が
挿入されている。 First, the specimen transport cassette 2 is loaded onto the packing 4 by the cassette transport device.
It should be noted that a specimen already smeared with blood by an automatic blood smearing unit is inserted into the specimen transport cassette 2.
そして、カセツト押え板５が移動して標本搬送
用カセツト２をパツキン４上にホールデイングす
る。このカセツト押え板５の押圧作用により、標
本搬送用カセツト２の底部がパツキン４に密着し
て封着され、染色液の槽が形成される。ここで染
色第１液１８ａが注入され、一定時間の後、排出
口の自動バルブ１３が開放されその液が排出され
る。液が完全に排出されると排出口の自動バルブ
１３が閉じられ次のバツフアー液２８ｂが注入さ
れ、以下同様に洗浄水１８ｎまでの液の注排が繰
り返えされ染色が行われる。 Then, the cassette holding plate 5 moves to hold the specimen transport cassette 2 on the packing 4. Due to the pressing action of the cassette holding plate 5, the bottom of the specimen transport cassette 2 is tightly sealed to the packing 4, thereby forming a tank for the staining solution. Here, the first dyeing liquid 18a is injected, and after a certain period of time, the automatic valve 13 at the discharge port is opened and the liquid is discharged. When the liquid is completely discharged, the automatic valve 13 at the discharge port is closed and the next buffer liquid 28b is injected, and thereafter, the liquid is repeatedly poured and drained up to the washing water 18n to perform staining.
このようにして染色が終了すると、カセツト押
え板５を除き標本搬送用カセツト２がアンローデ
イングされ、さらにカセツト移送装置により標本
搬送用カセツト２は検鏡ユニツト（図示しない）
に向けて移送される。検鏡ユニツトにおいては、
検鏡を行うまでの間に、標本搬送用カセツト２内
の標本に対し付着した洗浄水を乾燥させて取り除
いておく必要があるが、開口底部６を有するこの
標本搬送用カセツト２は通気性がよく、温風等を
吹き込むことにより速やかに乾燥を行うことがで
きる。 When the staining is completed in this manner, the specimen transport cassette 2 is unloaded with the cassette holding plate 5 removed, and the specimen transport cassette 2 is transferred to a speculum unit (not shown) by the cassette transport device.
will be transported towards. In the speculum unit,
Before microscopy, it is necessary to dry and remove the washing water adhering to the specimen in the specimen transport cassette 2, but this specimen transport cassette 2, which has an open bottom 6, is not breathable. Often, drying can be done quickly by blowing warm air or the like.
このように、この染色装置では、多量の標本を
収納できる標本移送容器と染色液槽を兼ねた標本
搬送用カセツトを具備し、染色液槽のデツドスペ
ースが極めて少なくなつている。また、多量標本
の染色処理時間が短縮され、しかも染色のための
染色液槽の形成や検鏡工程への標本搬送用カセツ
トの移送などを簡単に、かつ、円滑に行えるとい
う制御性に優れ、さらに機構簡易化に適し安価に
形成できる。 As described above, this staining apparatus is equipped with a specimen transport container capable of storing a large amount of specimens and a specimen transport cassette that also serves as a staining solution tank, and the dead space of the staining solution tank is extremely small. In addition, the staining process time for large numbers of specimens is shortened, and it has excellent controllability, allowing operations such as forming a staining solution tank for staining and transporting specimen transport cassettes to the microscopy process easily and smoothly. Furthermore, it is suitable for simplifying the mechanism and can be formed at low cost.
第１図はこの発明の実施例である染色装置全体
を示す斜視図、第２図は同染色装置を示す構造図
である。第３図および第４図は同染色装置による
染色工程動作を示すフローチヤートである。
１……血液塗沫標本、２……標本搬送用カセツ
ト、３……カセツト載置台、４……パツキン、５
……カセツト押え板、６……（標本搬送用カセツ
ト２の）開口底部、７……（染色液排出路１７
の）排出口、１３……自動バルブ、１７……染色
液排出路。
FIG. 1 is a perspective view showing the entire dyeing apparatus according to an embodiment of the present invention, and FIG. 2 is a structural diagram showing the dyeing apparatus. FIGS. 3 and 4 are flowcharts showing the dyeing process operation using the same dyeing apparatus. 1...Blood smear specimen, 2...Specimen transport cassette, 3...Cassette mounting table, 4...Packing, 5
...Cassette holding plate, 6...Opening bottom (of specimen transport cassette 2), 7... (staining solution discharge path 17)
) Discharge port, 13... Automatic valve, 17... Staining liquid discharge path.
Claims (1)
を備えた標本搬送用カセツトと、染色液排出路を
備えその染色液排出路の入口が表面に形成された
カセツト載置台と、前記標本搬送用カセツトの内
部と前記染色液排出路とが連通するように前記カ
セツト載置台に載置された前記標本搬送用カセツ
トの底部を封着する封着手段と、前記染色液排出
路の排出口を開閉する開閉手段とを有して成る染
色装置。1. A specimen transport cassette containing a blood smear specimen and having a staining solution outlet at the bottom, a cassette mounting table having a staining solution discharge path and an entrance for the staining solution discharge path formed on its surface, and the specimen. a sealing means for sealing the bottom of the specimen transport cassette placed on the cassette mounting table so that the interior of the transport cassette and the staining solution discharge path communicate with each other; and a discharge port of the staining solution discharge path. A dyeing device comprising an opening/closing means for opening and closing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57039637A JPS58155360A (en) | 1982-03-12 | 1982-03-12 | Dyeing equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57039637A JPS58155360A (en) | 1982-03-12 | 1982-03-12 | Dyeing equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58155360A JPS58155360A (en) | 1983-09-16 |
| JPH033182B2 true JPH033182B2 (en) | 1991-01-17 |
Family
ID=12558600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57039637A Granted JPS58155360A (en) | 1982-03-12 | 1982-03-12 | Dyeing equipment |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58155360A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5800784A (en) * | 1996-07-09 | 1998-09-01 | Horn; Marcus J. | Chemical sample treatment system and cassette, and methods for effecting multistep treatment process |
| JP6182130B2 (en) * | 2014-11-26 | 2017-08-16 | シスメックス株式会社 | Smear preparation apparatus and smear preparation method |
| CN116499844B (en) * | 2023-06-21 | 2023-09-15 | 遂宁市中心医院 | Automatic dyeing analysis device for pathological diagnosis |
-
1982
- 1982-03-12 JP JP57039637A patent/JPS58155360A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS58155360A (en) | 1983-09-16 |
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