JPH0367217B2 - - Google Patents
Info
- Publication number
- JPH0367217B2 JPH0367217B2 JP59151855A JP15185584A JPH0367217B2 JP H0367217 B2 JPH0367217 B2 JP H0367217B2 JP 59151855 A JP59151855 A JP 59151855A JP 15185584 A JP15185584 A JP 15185584A JP H0367217 B2 JPH0367217 B2 JP H0367217B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- chemical
- chemical solution
- chemical liquid
- cylinder pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000126 substance Substances 0.000 claims description 98
- 239000007788 liquid Substances 0.000 claims description 44
- 238000011282 treatment Methods 0.000 claims description 18
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 239000000523 sample Substances 0.000 description 105
- 239000000243 solution Substances 0.000 description 46
- 230000008595 infiltration Effects 0.000 description 10
- 238000001764 infiltration Methods 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 239000012472 biological sample Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000004809 Teflon Substances 0.000 description 4
- 229920006362 Teflon® Polymers 0.000 description 4
- 230000035515 penetration Effects 0.000 description 4
- 239000000057 synthetic resin Substances 0.000 description 4
- 229920003002 synthetic resin Polymers 0.000 description 4
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical group CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 3
- 239000003822 epoxy resin Substances 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 3
- 229920000647 polyepoxide Polymers 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 239000004850 liquid epoxy resins (LERs) Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011328 necessary treatment Methods 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- SYQBFIAQOQZEGI-UHFFFAOYSA-N osmium atom Chemical compound [Os] SYQBFIAQOQZEGI-UHFFFAOYSA-N 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、生物試料のごとき水分を含んだ試料
を電子顕微鏡用試料として作成してゆく課程にお
いて、上記試料に脱水や浸透処理を行なうための
装置に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention is a method for dehydrating and permeating a sample containing water, such as a biological sample, in the process of preparing the sample as a sample for an electron microscope. Regarding the device.
一般に、生物試料のごとき水分を含んだ試料を
電子顕微鏡用試料として作成するには、この試料
に対し合計13〜20工程にも及ぶ固定、脱水および
浸透処理が施される。
Generally, in order to prepare a sample containing water, such as a biological sample, as a sample for electron microscopy, the sample is subjected to a total of 13 to 20 fixation, dehydration, and infiltration processes.
その一例を示すと、生物試料はまずアルデヒド
固定（Aldehyde fixative）やオスミウム固定
（Osmium fixative）を施されたのち、水洗いさ
れてから、各種の濃度を有するエチルアルコール
溶液に浸漬されて試料内の水分をエチルアルコー
ルに置換され、その後このようして脱水処理を施
された試料はプロピレンオキサイド（Propylene
Oxide）液に浸漬されて上記試料内のエチルアル
コールをプロピレンオキサイドに置換される。 For example, a biological sample is first subjected to aldehyde fixative or osmium fixative, then washed with water, and then immersed in ethyl alcohol solutions of various concentrations to remove moisture within the sample. The sample was replaced with ethyl alcohol and then dehydrated in this way.
The ethyl alcohol in the sample is replaced with propylene oxide.
さらに、この試料はプロピレンとエポキシ樹脂
のごとき合成樹脂との混合液に浸漬されて浸透処
理を施されたのち、最終的には液状の合成樹脂と
してのエポキシ樹脂内に浸漬されて、試料内の水
分がすべてエポキシ樹脂に置換されるようになつ
ている。 Furthermore, this sample is immersed in a mixed solution of propylene and a synthetic resin such as epoxy resin for penetration treatment, and then finally immersed in epoxy resin, which is a liquid synthetic resin. All water is replaced by epoxy resin.
なお、上述の固定、脱水および浸透処理は、各
工程ごとに試料を浸漬する時間や温度等が設定さ
れている。 Note that in the above-mentioned fixation, dehydration, and infiltration treatments, the time and temperature at which the sample is immersed are set for each step.
このようにして浸透処理等を施された生物試料
は、その内部に水分を含まないため、長時間の保
存に耐え、しかも電子顕微鏡用試料として最適の
ものになる。 Biological samples that have been subjected to osmosis treatment in this way do not contain water, so they can withstand long-term storage and are ideal as samples for electron microscopy.
〔従来の技術〕
以上のような試料の浸透処理手段としては、従
来、第７図に示すようなものがある（特公昭57−
19372号公報参照）。[Prior Art] Conventionally, there is a method for penetrating a sample as described above, as shown in Figure 7 (Japanese Patent Publication No. 57-
(See Publication No. 19372).
すなわち、この浸透処理手段にあつては、透明
ガラス等の透明材から成り同一形状を有する複数
の円筒状薬液槽１が、薬液レベル１ａよりも上方
で、透明ガラス等の透明材から成る逆Ｕ字管２を
介して、互いに連結されている。 That is, in this infiltration treatment means, a plurality of cylindrical chemical liquid tanks 1 made of a transparent material such as transparent glass and having the same shape are arranged above the chemical liquid level 1a in an inverted U shape made of a transparent material such as transparent glass. They are connected to each other via a tube 2.
また、各薬液槽１内には、この薬液槽１との間
の薬液の連通を許容しうる連通口としての孔を多
数個有する孔材から成る試料案内路としてのＵ字
管１ｂが配設されており、これより生物試料等の
試料を薬液槽１内におけるエチルアルコールやプ
ロピレンオキサイドや液状のエポキシ樹脂等のご
とき薬液に浸漬させながら、該試料を案内できる
ようになつている。 Further, in each chemical liquid tank 1, a U-shaped tube 1b is provided as a sample guide path made of a porous material having a large number of holes as communication ports that allow communication of the chemical liquid with this chemical liquid tank 1. This makes it possible to guide a sample such as a biological sample while immersing it in a chemical solution such as ethyl alcohol, propylene oxide, or liquid epoxy resin in the chemical solution tank 1.
さらに、多孔材から成るＵ字管１ｂの上端は逆
Ｕ字管２に接続されている。 Further, the upper end of the U-shaped tube 1b made of a porous material is connected to an inverted U-shaped tube 2.
そして、この装置には、試料を各Ｕ字管１ｂお
よび逆Ｕ字管２に沿い駆動して順次、固定、脱
水、および浸透を行なわせるための試料搬送手段
が設けられている。 This apparatus is provided with sample transport means for driving the sample along each U-shaped tube 1b and inverted U-shaped tube 2 to sequentially fix, dehydrate, and permeate the sample.
この試料搬送手段は、チエーンやワイヤロープ
のごとき可撓性条体４をそなえており、この条件
４は薬液の流通を許容し、その内部に試料を収納
した容器３に連結されて、各薬液槽１のＵ字管１
ｂおよび逆Ｕ字管２を通過すべく、無端状に配設
されている。 This sample transport means is equipped with a flexible strip 4 such as a chain or a wire rope, and this condition 4 allows the flow of a chemical solution. U-shaped pipe 1 of tank 1
b and the inverted U-shaped tube 2, and is arranged in an endless manner.
さらに、この条体４はスプロケツトホイールま
たはプーリのごとき動力伝達輪５を介して駆動機
構の主要部をなす電動機６により駆動せしめら
れ、これにより容器３を移動させることができる
ようになつている。 Furthermore, this strip 4 is driven by an electric motor 6, which is the main part of the drive mechanism, via a power transmission wheel 5 such as a sprocket wheel or a pulley, thereby making it possible to move the container 3. .
そして、この浸透処理装置は、電動機６を駆動
させることにより、条体４に連結された容器３を
多孔材製Ｕ字管１ｂ内で移動させ、次々と薬液槽
内に浸漬させることにより、容器３内に収納され
た試料を脱水、浸透処理するものである。 This infiltration treatment apparatus moves the containers 3 connected to the strips 4 within the U-shaped tube 1b made of porous material by driving the electric motor 6, and immerses the containers one after another into the chemical tank. This is to dehydrate and infiltrate the sample stored in the chamber.
また処理しようとする試料を薬液の流入口と流
出口とを備えた槽内に置き、試料処理用薬液を定
められたプログラムにより、順次槽内に送り込ん
で所要時間づつ処理するものである。 In addition, the sample to be processed is placed in a tank having an inlet and an outlet for the chemical solution, and the sample processing chemicals are sequentially fed into the tank according to a predetermined program and processed for a required period of time.
しかしながら、以上のような浸透処理装置にあ
つては、試料を１個づつ容器に収納して処理する
必要があるため、一度に処理できる試料の数は少
なく、その結果、処理後における試料の均一性が
得られないという不具合がある。また、試料を１
個づつ容器に収納する必要があるため、処理装置
には必ず作業者が付いて処理された試料を取り出
し、次に処理すべき試料を容器に収納しなければ
ならず、その作業が煩わしいものであつた。
However, in the case of the above-mentioned infiltration treatment equipment, it is necessary to store each sample in a container and process it, so the number of samples that can be processed at one time is small, and as a result, the uniformity of the sample after treatment is reduced. There is a problem that you cannot get sex. In addition, 1 sample
Since each sample must be stored in a container, a worker must be present at the processing equipment to take out the processed sample and store the next sample to be processed in the container, which is a cumbersome task. It was hot.
また、上記浸透処理装置は薬液槽内に試料を浸
漬するものであるから薬液層は常に多量の薬液で
満たされている必要があり、薬液の交換作業を頻
繁に行なわなければならないという問題があつ
た。更に、薬液槽内を、試料を収納した容器が移
動すると、薬液から気泡が発生し易いために良好
な浸透処理ができない場合がある。 In addition, since the above-mentioned infiltration treatment apparatus immerses the sample in a chemical tank, the chemical layer must always be filled with a large amount of chemical, and there is a problem in that the chemical must be replaced frequently. Ta. Furthermore, if a container containing a sample moves within the chemical solution tank, bubbles are likely to be generated from the chemical solution, making it impossible to perform a good infiltration treatment.
また試料を処理槽内に置き、薬液を定められた
プログラムにより槽内に送り込んで処理するもの
では、薬液槽からの薬液の吸引や、試料収納部へ
の薬液の送り出しの際、薬液量の微妙なコントロ
ールができないため、試料への薬液の浸透圧力を
適正に制御できず、薬液内に空気やガスが残留し
て浸透効果が充分でないという問題があつた。 In addition, when a sample is placed in a processing tank and a chemical solution is sent into the tank according to a predetermined program for processing, there may be a slight difference in the amount of chemical solution when suctioning the chemical solution from the chemical solution tank or sending the chemical solution to the sample storage section. Therefore, there was a problem in that the osmotic pressure of the chemical solution into the sample could not be properly controlled, and air and gas remained in the chemical solution, resulting in insufficient osmotic effect.
本発明は、上記事情に鑑みてなされたものであ
り、その手段は、試料の脱水、浸透処理をするた
めの薬液を入れるべき複数の薬液槽と、処理すべ
き複数の試料を収納可能な複数の試料収納部と、
前記薬液槽内の薬液を吸引し吸引された薬液を前
記試料収納部に送り出すために切換弁を介して該
試料収納部と該薬液槽とに連通されたシリンダー
ポンプと、薬液の使用順位、使用量及び処理時間
を制御するために前記切換弁と前記シリンダーポ
ンプとを制御する制御部と、前記試料収納部に送
られた薬液中の気体を除去するための真空ポンプ
とを有するようにしたものである。
The present invention has been made in view of the above circumstances, and its means include a plurality of chemical liquid tanks into which chemical liquids are to be placed for dehydration and osmosis treatment of samples, and a plurality of chemical liquid tanks capable of storing a plurality of samples to be treated. a sample storage section,
A cylinder pump that is connected to the sample storage section and the chemical tank via a switching valve in order to aspirate the chemical solution in the chemical solution tank and send the sucked chemical solution to the sample storage section; A control unit that controls the switching valve and the cylinder pump to control the amount and processing time, and a vacuum pump that removes gas from the chemical solution sent to the sample storage unit. It is.
以下、添付図面に基づいて本発明の実施例を説
明する。
Embodiments of the present invention will be described below based on the accompanying drawings.
第１図は本発明の実施例を示すものである。こ
の試料浸透処理装置（以下、処理装置）は、試料
の脱水、浸透処理をするための薬液７（第１図中
７ａ，７ｂで示す）を入れた複数個の薬液槽８
（第１図中８ａ，８ｂで示す）と、処理すべき試
料９を入れた試料収納部１０と、薬液７を吸入、
排出し薬液を流動させるポンプ、例えばシリンダ
ーポンプ１１とが、薬液の流路を変更させる切換
弁１２を介して配管１３によつて相互に連結され
ている。 FIG. 1 shows an embodiment of the invention. This sample permeation treatment apparatus (hereinafter referred to as treatment apparatus) includes a plurality of chemical liquid tanks 8 containing chemical liquids 7 (indicated by 7a and 7b in FIG. 1) for dehydrating and permeating samples.
(shown as 8a and 8b in FIG. 1), a sample storage section 10 containing a sample 9 to be treated, and a drug solution 7 for inhalation.
Pumps that discharge and flow the chemical solution, such as cylinder pumps 11, are interconnected by piping 13 via a switching valve 12 that changes the flow path of the chemical solution.
上記薬液槽８には、例えばエチルアルコールや
プロピレンオキサイドのように試料９の脱水、浸
透処理に用いるべき薬液が入れられている。 The chemical solution tank 8 contains a chemical solution, such as ethyl alcohol or propylene oxide, to be used for dehydration and penetration treatment of the sample 9.
次に、上記試料収納部１０は、試料９をれるべ
き多数の収納孔１４を有する断面矩形状の試料ホ
ルダー１５と、該試料ホルダー１５を収納する箱
状の試料室１６とを備えるものである。試料ホル
ダー１５は、非接着性の材質、例えばテフロン等
で形成されており、第２図および第３図に示すよ
うに、上記試料孔１４の上端傾斜部には試料を識
別するための番号等の符号１７が凸状又は凹状に
施され、最終的に合成樹脂を流し込んで試料を固
めたときに、符号１７が樹脂に型どられ、試料９
を識別できるようになつている。また、試料ホル
ダー１５の上面には薬液７が流入したときに試料
９が試料孔１４から飛び出さないようにメツシユ
１８が載置され、他方、試料ホルダー１５の下面
には試料の脱落を防止するためのメツシユ１９が
張られている。この試料ホルダー１５は、処理す
べき試料の数に応じて第４図ないし第６図に示す
ような、各種の大きさのものを用いることが可能
である。そして、この試料ホルダー１５は、その
外周を覆うホルダーケース２０に収められて、試
料室１６に収納される。尚、試料室１６内には、
薬液７が勢いよく試料室１６内に流入するのを防
止し薬液が均等に拡散するように整流板２１が設
けられている。また、試料室１６はその上部が着
脱自在の上蓋２２によつて覆われており、該上蓋
２２および導管２３を介して真空ポンプ２４と連
通している。 Next, the sample storage section 10 includes a sample holder 15 with a rectangular cross section and a large number of storage holes 14 into which the sample 9 can be placed, and a box-shaped sample chamber 16 that accommodates the sample holder 15. . The sample holder 15 is made of a non-adhesive material such as Teflon, and as shown in FIGS. 2 and 3, a number or the like for identifying the sample is provided on the upper slope of the sample hole 14. The code 17 is formed into a convex or concave shape, and when the synthetic resin is finally poured into the sample and hardened, the code 17 is molded into the resin, and the sample 9
It has become possible to identify Further, a mesh 18 is placed on the top surface of the sample holder 15 to prevent the sample 9 from jumping out from the sample hole 14 when the chemical solution 7 flows in, and a mesh 18 is placed on the bottom surface of the sample holder 15 to prevent the sample from falling out. A mesh 19 has been put up for this purpose. The sample holder 15 can be of various sizes as shown in FIGS. 4 to 6 depending on the number of samples to be processed. The sample holder 15 is housed in a holder case 20 that covers its outer periphery, and is housed in the sample chamber 16. In addition, inside the sample chamber 16,
A rectifying plate 21 is provided to prevent the chemical liquid 7 from flowing into the sample chamber 16 with force and to spread the chemical liquid evenly. Further, the upper part of the sample chamber 16 is covered by a removable upper lid 22, and communicates with a vacuum pump 24 via the upper lid 22 and a conduit 23.
ところで、シリンダーポンプ１１および配管１
３内の薬液の流路を切換える切換弁１２は制御装
置２５によつて制御される。すなわち、制御装置
２５は、シリンダーポンプ１１および切換弁１２
を制御して使用すべき薬液７の順番と使用量およ
び夫々の薬液ごとに予め設定されている処理時間
等を調節し浸透処理を進行させるものである。ま
た制御装置２５は、真空ポン２４の圧力制御およ
びリークさせるときの開放弁２６の制御も行なう
ものである。そして、かかる制御の状態は制御装
置２５に接続された表示装置２７に表示され、使
用中の薬液の種類、その設定処理時間および経過
時間、その処理工程の把握に必要な情報（例えば
室温等）を知ることができるようになつている。 By the way, cylinder pump 11 and piping 1
A switching valve 12 that switches the flow path of the chemical liquid in the chemical liquid is controlled by a control device 25. That is, the control device 25 controls the cylinder pump 11 and the switching valve 12.
The infiltration process is progressed by controlling the order and amount of the chemical liquids 7 to be used, the processing time set in advance for each chemical liquid, etc. The control device 25 also controls the pressure of the vacuum pump 24 and the release valve 26 when leaking. The status of this control is displayed on the display device 27 connected to the control device 25, and information necessary to understand the type of chemical solution in use, its set processing time and elapsed time, and the processing process (for example, room temperature, etc.) It is now possible to know.
以上のように構成された処理装置の作用を説明
する。 The operation of the processing device configured as above will be explained.
処理工程の最初に、例えば薬液７ａが使用され
るものとすると、制御装置２５からの信号に基づ
いて、切換弁１２がシリンダーポンプ１１と薬液
槽８ａを開状態として連通させ、他への流路を閉
状態とする。次に、制御装置２４からの信号に基
づいてシリンダーポンプ１１が作動し、薬液７ａ
を吸引する。この時、吸引される薬液７ａの量
は、試料ホルダー１５の大きさ乃至処理すべき試
料の数によつて予め制御装置に設定されている。
そして、薬液７ａがシリンダーポンプ１１に吸引
されると、切換弁１２がシリンダーポンプ１１と
試料室１６とを開状態として連通させ、他への流
路を閉状態とする。この状態でシリンダーポンプ
１１が薬液７ａを排出することにより、薬液７ａ
が試料室１６へ送られることになる。尚、試料室
１６に送られた薬液７ａは試料ホルダー１５およ
びメツシユ１９の下側に設けられた整流板２１に
よつて試料室１６の全体に平均的に拡散し、試料
９を均一に浸透させるようになつている。また、
薬液７ａは試料９を浸透し、更に試料ホルダー１
５の収納孔１４の上部に達するまで試料室１６を
満たすようになつている。尚、薬液が流動する場
合には、従来の処理装置のように薬液内を試料が
移動する場合に比べて薬液に気泡が生ずることは
少ないが、この処理装置においては、真空ポンプ
２４を設け薬液７ａを十分送つた後、それまで開
放していた開放弁２６を閉じ、真空ポンプ２４を
作動させることによつて、薬液７ａ内の空気やガ
スを吸引するようになつている。また、このとき
シリンダーポンプ１１を微動させて試料室１６内
の薬液を動かすと薬液中の空気やガス完全に取り
除かれ、浸透効果が増加する。 For example, when the chemical liquid 7a is used at the beginning of the treatment process, the switching valve 12 opens and communicates the cylinder pump 11 and the chemical liquid tank 8a based on a signal from the control device 25, and connects the cylinder pump 11 and the chemical liquid tank 8a to other channels. is closed. Next, the cylinder pump 11 is activated based on a signal from the control device 24, and the chemical liquid 7a is activated.
aspirate. At this time, the amount of the chemical liquid 7a to be aspirated is preset in the control device depending on the size of the sample holder 15 or the number of samples to be processed.
When the chemical solution 7a is sucked into the cylinder pump 11, the switching valve 12 opens the cylinder pump 11 and the sample chamber 16 to communicate with each other, and closes the flow path to other parts. In this state, the cylinder pump 11 discharges the chemical liquid 7a, thereby causing the chemical liquid 7a to
will be sent to the sample chamber 16. The chemical solution 7a sent to the sample chamber 16 is evenly diffused throughout the sample chamber 16 by the rectifier plate 21 provided below the sample holder 15 and the mesh 19, allowing the sample 9 to penetrate uniformly. It's becoming like that. Also,
The chemical solution 7a permeates the sample 9 and further penetrates the sample holder 1.
The sample chamber 16 is filled until it reaches the upper part of the storage hole 14 of No. 5. Note that when the chemical solution flows, bubbles are less likely to be generated in the chemical solution compared to when the sample moves within the chemical solution as in conventional processing equipment. After sufficiently sending 7a, the open valve 26, which had been open until then, is closed and the vacuum pump 24 is operated, thereby sucking air or gas in the chemical solution 7a. Further, at this time, if the cylinder pump 11 is slightly moved to move the chemical liquid in the sample chamber 16, air and gas in the chemical liquid are completely removed, increasing the permeation effect.
一方、浸漬する必要な時間が経過すると、開放
弁２６が開くと共にシリンダーポンプ１１が作動
して、試料室１６内の薬液７ａを吸入する。そし
て、切換弁１２がシリンダーポンプ１１と薬液槽
８ａを開通させ他の流路を閉じた後、シリンダー
ポンプ１１が薬液７ａを排出することにより、薬
液７ａは薬液槽８ａに戻される。 On the other hand, when the required immersion time has elapsed, the release valve 26 opens and the cylinder pump 11 operates to suck in the chemical solution 7a in the sample chamber 16. After the switching valve 12 opens the cylinder pump 11 and the chemical tank 8a and closes other channels, the cylinder pump 11 discharges the chemical liquid 7a, so that the chemical liquid 7a is returned to the chemical liquid tank 8a.
続いて、薬液７ｂが使用される番となり、切換
弁１２が薬液槽８ｂとシリンダーポンプ１１とを
開通し、他の流路を閉じると、シリンダーポンプ
１１が薬液８ｂを吸入し、それ以降、以上と同様
の処理工程が繰り返されることにより必要とされ
る処理工程が終了する。 Next, it is the turn of the chemical solution 7b to be used, and when the switching valve 12 opens the chemical solution tank 8b and the cylinder pump 11 and closes the other channels, the cylinder pump 11 sucks the chemical solution 8b, and from then on, the above The necessary treatment steps are completed by repeating the same treatment steps.
尚、上記処理装置による必要な処理工程が終了
した後の処理を説明すると、まず試料室１６の上
蓋２２を外し、ホルダーケース２０を取り出す。
そして上側のメツシユ１８を外してホルダーケー
ス２０の上にテフロンの板を載せる。その状態
で、ホルダーケース２０をひつくり返すとテフロ
ン板の上に試料ホルダー１５と下側のメツシユ１
９が落ち、ホルダーケース２０とメツシユ１９を
外すと試料９の入つた試料ホルダー１５がテフロ
ン板の上に残る。そこへ合成樹脂を流し込み固め
た後、１個づつ抜きとつて、全処理作業が終了す
る。尚、試料ホルダー１５の試料９を入れる収納
孔１４の上部には、記号や番号などの符号１７が
凸状又は凹状に施されているから、処理された各
試料を容易に識別できるようになつている。 Incidentally, to explain the processing after the necessary processing steps by the above-mentioned processing apparatus are completed, first, the upper cover 22 of the sample chamber 16 is removed and the holder case 20 is taken out.
Then, remove the upper mesh 18 and place a Teflon plate on the holder case 20. In this state, when the holder case 20 is turned over, the sample holder 15 and the lower mesh 1 are placed on the Teflon plate.
9 falls, and when the holder case 20 and mesh 19 are removed, the sample holder 15 containing the sample 9 remains on the Teflon plate. After pouring synthetic resin into it and solidifying it, the pieces are removed one by one, completing the entire process. Incidentally, a code 17 such as a symbol or number is provided in a convex or concave shape on the upper part of the storage hole 14 into which the sample 9 of the sample holder 15 is placed, so that each processed sample can be easily identified. ing.
尚、上記実施例においては、試料の収納部を試
料室と試料ホルダー等から構成されるものとして
説明したが、本発明においては、複数の試料を収
納し、薬液をシリンダーポンプにより制御しなが
ら送込んで試料の浸透処理ができるものであれ
ば、上記実施例のような試料収納部である必要は
ない。また、薬液槽の数は必要に応じて適宜変更
することが可能である。 In the above embodiment, the sample storage section was explained as being composed of a sample chamber, a sample holder, etc., but in the present invention, a plurality of samples are stored and the chemical solution is sent while being controlled by a cylinder pump. It does not need to be a sample storage part like the one in the above embodiment, as long as the sample can be permeated in an intensive manner. Further, the number of chemical liquid tanks can be changed as necessary.
以上説明したように、本発明に係る試料浸透処
理装置によれば、多数の試料を同時にかつ自動的
に処理できるので、各試料を均一に処理できる。
また、処理作業中に作業者が装置に付いている必
要はなくなり、従来の装置における試料の取り外
し、収納の煩わしさがなくなつた。
As explained above, according to the sample infiltration processing apparatus according to the present invention, a large number of samples can be processed simultaneously and automatically, so that each sample can be processed uniformly.
Furthermore, there is no longer a need for an operator to be present with the apparatus during processing operations, eliminating the trouble of removing and storing samples in conventional apparatuses.
また薬液槽内の薬液を試料収納部に送り出すた
めのシリンダーポンプと、薬液の使用順位、使用
量及び処理時間を制御するために前記切換弁と前
記シリンダーポンプとを制御する制御部とを設け
たので、薬液槽からの薬液の吸引や、試料収納部
への薬液の送り出しの際、薬液量の微妙なコント
ロールが可能となる。またシリンダーポンプの制
御により、試料収納部に送り込む液体の圧力の微
妙なコントロールができるうになつて、試料への
薬液の浸透圧力を適正に制御できる。さらにシリ
ンダーポンプを微動させ試料内の薬液を動かし
て、薬液内に空気やガスが取り除かれ、浸透効果
を高めることができると言う効果がある。 Further, a cylinder pump for sending the chemical solution in the chemical solution tank to the sample storage section, and a control section for controlling the switching valve and the cylinder pump in order to control the usage order, usage amount, and processing time of the chemical solution are provided. Therefore, it is possible to delicately control the amount of the chemical liquid when sucking the chemical liquid from the chemical liquid tank or sending the chemical liquid to the sample storage section. Furthermore, by controlling the cylinder pump, it becomes possible to delicately control the pressure of the liquid sent into the sample storage section, and the osmotic pressure of the chemical solution into the sample can be appropriately controlled. Furthermore, by slightly moving the cylinder pump to move the chemical liquid within the sample, air and gas are removed from the chemical liquid, which has the effect of increasing the penetration effect.
しかも本願では、さらに真空ポンプを設けたの
で、シリンダーポンプとの相乗効果により、液体
中の空気やガスが取り除くことができ、前述した
浸透効果をより高めるこができる。また、薬液自
体を移動させることにより、異つた薬液が相互に
混つて試料に浸透することも試料を良好すること
に資するものである。 Moreover, in the present application, since a vacuum pump is further provided, air and gas in the liquid can be removed due to the synergistic effect with the cylinder pump, and the above-mentioned permeation effect can be further enhanced. Further, by moving the chemical liquid itself, different chemical liquids can mix with each other and penetrate into the sample, which also contributes to improving the quality of the sample.
しかも本発明によれば、試料収納部内の試料を
浸漬するに必要最小限の薬液を使用すれば良いか
ら、薬液の使用量を抑えることができ、その結
果、薬液の交換回数も減少して、交換作業の煩わ
しさを減らすことができる。また、試料収納部内
に収納する試料の数が少ないときには薬液の使用
量を少量とすることができるので、薬液の節約が
できるものである。 Moreover, according to the present invention, it is sufficient to use the minimum amount of chemical solution necessary to immerse the sample in the sample storage section, so the amount of chemical solution used can be reduced, and as a result, the number of times the chemical solution is replaced is also reduced. The hassle of replacement work can be reduced. Further, when the number of samples stored in the sample storage section is small, the amount of chemical solution used can be reduced, so that the amount of chemical solution can be saved.
第１図は本発明の実施例に係る試料浸透処理装
置を示す断面説明図、第２図は上記実施例に係る
試料収納部の試料ホルダーを示す断面図、第３図
は上記試料ホルダーに設けられた収納孔を示す斜
視図、第４図ないし第６図は、上記実施例におい
て使用される各種の試料ホルダーを示す断面図、
第７図は従来の試料浸透処理装置を示す断面図で
ある。
８……薬液槽、９……試料、１０……試料収納
部、１１……シリンダーポンプ、１２……切換
弁、１３……導管、２４……真空ポンプ、２５…
…制御部。
FIG. 1 is a cross-sectional explanatory diagram showing a sample infiltration treatment apparatus according to an embodiment of the present invention, FIG. 2 is a cross-sectional diagram showing a sample holder of a sample storage section according to the above embodiment, and FIG. FIGS. 4 to 6 are cross-sectional views showing various sample holders used in the above embodiments,
FIG. 7 is a sectional view showing a conventional sample penetration treatment device. 8... Chemical tank, 9... Sample, 10... Sample storage section, 11... Cylinder pump, 12... Switching valve, 13... Conduit, 24... Vacuum pump, 25...
...control section.
Claims (1)
れるべき複数の薬液槽と、処理すべき複数の試料
を収納可能な複数の試料収納部と、前記薬液槽内
の薬液を吸引し吸引された薬液を前記試料収納部
に送り出すために切換弁を介して該試料収納部と
該薬液槽とに連通されたシリンダーポンプと、薬
液の使用順位、使用量及び処理時間を制御するた
めに前記切換弁と前記シリンダーポンプとを制御
する制御部と、前記試料収納部に送られた薬液中
の気体を除去するための真空ポンプとを有するこ
とを特徴とする試料浸透処理装置。1. A plurality of chemical liquid tanks into which chemical liquids for dehydration and osmosis treatment of samples are to be placed, a plurality of sample storage parts capable of storing a plurality of samples to be processed, and a suction device for sucking the chemical liquid in the chemical liquid tanks. a cylinder pump communicating with the sample storage section and the chemical tank via a switching valve to send the chemical solution to the sample storage section; and the switching valve to control the usage order, usage amount, and processing time of the chemical solution. and a control section for controlling the cylinder pump, and a vacuum pump for removing gas in the chemical solution sent to the sample storage section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15185584A JPS6129734A (en) | 1984-07-21 | 1984-07-21 | Processor for sample penetration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15185584A JPS6129734A (en) | 1984-07-21 | 1984-07-21 | Processor for sample penetration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6129734A JPS6129734A (en) | 1986-02-10 |
| JPH0367217B2 true JPH0367217B2 (en) | 1991-10-22 |
Family
ID=15527731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15185584A Granted JPS6129734A (en) | 1984-07-21 | 1984-07-21 | Processor for sample penetration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6129734A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4810471A (en) * | 1986-07-24 | 1989-03-07 | Rohm And Haas Co. | Vacuum manifold for extraction processing |
| JPH0221238A (en) * | 1988-07-08 | 1990-01-24 | Power Reactor & Nuclear Fuel Dev Corp | Resin injector for preparing metal phase sample |
| CA2441254A1 (en) | 2001-03-16 | 2002-09-26 | Memorec Biotec Gmbh | Examination device and method for examination of chemical and/or biological samples |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5670440A (en) * | 1979-11-14 | 1981-06-12 | Chiyoda Seisakusho:Kk | Device for embedding treatment of living-body organization |
| JPS56150325A (en) * | 1980-04-22 | 1981-11-20 | Yukitaka Ajimi | Vaccum device |
| JPS57103031A (en) * | 1981-10-30 | 1982-06-26 | Akashi Seisakusho Co Ltd | Sample osmosis treatment device |
-
1984
- 1984-07-21 JP JP15185584A patent/JPS6129734A/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5670440A (en) * | 1979-11-14 | 1981-06-12 | Chiyoda Seisakusho:Kk | Device for embedding treatment of living-body organization |
| JPS56150325A (en) * | 1980-04-22 | 1981-11-20 | Yukitaka Ajimi | Vaccum device |
| JPS57103031A (en) * | 1981-10-30 | 1982-06-26 | Akashi Seisakusho Co Ltd | Sample osmosis treatment device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6129734A (en) | 1986-02-10 |
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