JPS61296254A - 電気泳動用媒体材料 - Google Patents
電気泳動用媒体材料Info
- Publication number
- JPS61296254A JPS61296254A JP60139678A JP13967885A JPS61296254A JP S61296254 A JPS61296254 A JP S61296254A JP 60139678 A JP60139678 A JP 60139678A JP 13967885 A JP13967885 A JP 13967885A JP S61296254 A JPS61296254 A JP S61296254A
- Authority
- JP
- Japan
- Prior art keywords
- sheet
- electrophoresis
- cover sheet
- medium material
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000463 material Substances 0.000 title claims abstract description 65
- 238000001962 electrophoresis Methods 0.000 claims abstract description 83
- 239000012528 membrane Substances 0.000 claims description 66
- 239000011148 porous material Substances 0.000 claims description 11
- 229920000642 polymer Polymers 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 3
- 229920000515 polycarbonate Polymers 0.000 claims description 3
- 239000004417 polycarbonate Substances 0.000 claims description 3
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 claims description 2
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 claims description 2
- 229910052731 fluorine Inorganic materials 0.000 claims description 2
- 239000011737 fluorine Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims 1
- 238000003860 storage Methods 0.000 abstract description 10
- 238000010981 drying operation Methods 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 238000002601 radiography Methods 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 27
- 238000000034 method Methods 0.000 description 25
- 238000000376 autoradiography Methods 0.000 description 19
- 238000004458 analytical method Methods 0.000 description 17
- 108010025899 gelatin film Proteins 0.000 description 10
- -1 polytetrafluoroethylene, tetrafluoroethylene-hexafluoropropylene copolymers Polymers 0.000 description 10
- 125000006850 spacer group Chemical group 0.000 description 9
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 238000001035 drying Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 3
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 3
- 238000005266 casting Methods 0.000 description 3
- 229920002301 cellulose acetate Polymers 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 229920000573 polyethylene Polymers 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 239000011837 N,N-methylenebisacrylamide Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000727732 Tokoyo Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000003486 chemical etching Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- UUAGAQFQZIEFAH-UHFFFAOYSA-N chlorotrifluoroethylene Chemical group FC(F)=C(F)Cl UUAGAQFQZIEFAH-UHFFFAOYSA-N 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000003851 corona treatment Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000028659 discharge Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005520 electrodynamics Effects 0.000 description 1
- 238000000866 electrolytic etching Methods 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 238000011328 necessary treatment Methods 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 239000003505 polymerization initiator Substances 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000011882 ultra-fine particle Substances 0.000 description 1
- 239000002966 varnish Substances 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60139678A JPS61296254A (ja) | 1985-06-25 | 1985-06-25 | 電気泳動用媒体材料 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60139678A JPS61296254A (ja) | 1985-06-25 | 1985-06-25 | 電気泳動用媒体材料 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61296254A true JPS61296254A (ja) | 1986-12-27 |
| JPH043823B2 JPH043823B2 (enrdf_load_stackoverflow) | 1992-01-24 |
Family
ID=15250872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60139678A Granted JPS61296254A (ja) | 1985-06-25 | 1985-06-25 | 電気泳動用媒体材料 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61296254A (enrdf_load_stackoverflow) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49126237A (enrdf_load_stackoverflow) * | 1973-04-03 | 1974-12-03 | ||
| JPS5531418A (en) * | 1978-08-25 | 1980-03-05 | Dainichi Seikan Kk | Corrosion resistant coating layer on metal container |
| JPS6114558A (ja) * | 1984-06-30 | 1986-01-22 | Fuji Photo Film Co Ltd | 電気泳動用媒体材料 |
-
1985
- 1985-06-25 JP JP60139678A patent/JPS61296254A/ja active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49126237A (enrdf_load_stackoverflow) * | 1973-04-03 | 1974-12-03 | ||
| JPS5531418A (en) * | 1978-08-25 | 1980-03-05 | Dainichi Seikan Kk | Corrosion resistant coating layer on metal container |
| JPS6114558A (ja) * | 1984-06-30 | 1986-01-22 | Fuji Photo Film Co Ltd | 電気泳動用媒体材料 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH043823B2 (enrdf_load_stackoverflow) | 1992-01-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU762384B2 (en) | Encapsulated IPG strips | |
| US4006069A (en) | Support for electrophoretic analysis | |
| EP0043287B1 (en) | Electrophoretic gel container | |
| JPS62184343A (ja) | 電気泳動装置 | |
| JPS60231152A (ja) | 電気泳動のための方法及び装置 | |
| US4622124A (en) | Device for horizontal electroblotting | |
| WO1996009537A1 (en) | Plastic mold for electrophoresis gel | |
| JPH0713205A (ja) | 電気泳動ゲルプレートならびに電気泳動ゲルプレートパッケージおよびそれを作る方法 | |
| CN104321646B (zh) | 电泳系统以及分离和识别方法 | |
| JPS59126237A (ja) | 電気泳動分析用材料 | |
| US4722777A (en) | Improvement of electrophoretic element using polyacrylamide gel | |
| EP0159830A1 (en) | Process of manufacturing element for electrophoresis and apparatus therefor | |
| CN104364640B (zh) | 制造电泳盒的方法 | |
| CN104335034B (zh) | 具有至少一个可移除区段的电泳凝胶盒 | |
| JPS61296254A (ja) | 電気泳動用媒体材料 | |
| CN104321643A (zh) | 包括附连到支承件上的扁平凝胶部件的电泳凝胶单元 | |
| US4750506A (en) | Apparatus for processing a slab gel | |
| JP2550801Y2 (ja) | 電気泳動装置 | |
| US20060076237A1 (en) | Coated hydrophilic membrances for electrophoresis applications | |
| JPS61296255A (ja) | 電気泳動分析用材料 | |
| US4810341A (en) | Method of making electrophoresis sheet | |
| JPH0954066A (ja) | 電気泳動後のポリアクリルアミドゲルの乾燥方法 | |
| JPS58105053A (ja) | 二次元電気泳動法 | |
| JP2780334B2 (ja) | 電気泳動装置 | |
| JPS63262553A (ja) | 電気泳動器具 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |