JPS61282069A - ビフェニル代謝機能を備えた形質転換微生物シュウドモナス・エルギノ−サｋｆ２５７株 - Google Patents
ビフェニル代謝機能を備えた形質転換微生物シュウドモナス・エルギノ−サｋｆ２５７株Info
- Publication number
- JPS61282069A JPS61282069A JP60124554A JP12455485A JPS61282069A JP S61282069 A JPS61282069 A JP S61282069A JP 60124554 A JP60124554 A JP 60124554A JP 12455485 A JP12455485 A JP 12455485A JP S61282069 A JPS61282069 A JP S61282069A
- Authority
- JP
- Japan
- Prior art keywords
- biphenyl
- strain
- transformed
- pseudomonas aeruginosa
- function
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 title claims abstract description 40
- 235000010290 biphenyl Nutrition 0.000 title claims abstract description 20
- 239000004305 biphenyl Substances 0.000 title claims abstract description 20
- 241000589517 Pseudomonas aeruginosa Species 0.000 title claims abstract 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 20
- 239000013612 plasmid Substances 0.000 claims abstract description 12
- 241000588724 Escherichia coli Species 0.000 claims abstract description 7
- 241000589630 Pseudomonas pseudoalcaligenes Species 0.000 claims abstract description 3
- 244000005700 microbiome Species 0.000 claims description 10
- 230000007102 metabolic function Effects 0.000 claims description 5
- 108091008146 restriction endonucleases Proteins 0.000 abstract description 7
- 241000589516 Pseudomonas Species 0.000 abstract description 3
- 238000003776 cleavage reaction Methods 0.000 abstract description 3
- 230000007017 scission Effects 0.000 abstract description 3
- 230000002070 germicidal effect Effects 0.000 abstract 1
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
- 239000000872 buffer Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000013611 chromosomal DNA Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000007993 MOPS buffer Substances 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000004020 Oxygenases Human genes 0.000 description 2
- 108090000417 Oxygenases Proteins 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- YKOQAAJBYBTSBS-UHFFFAOYSA-N biphenyl-2,3-diol Chemical compound OC1=CC=CC(C=2C=CC=CC=2)=C1O YKOQAAJBYBTSBS-UHFFFAOYSA-N 0.000 description 2
- UBXYXCRCOKCZIT-UHFFFAOYSA-N biphenyl-3-ol Chemical group OC1=CC=CC(C=2C=CC=CC=2)=C1 UBXYXCRCOKCZIT-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 210000003495 flagella Anatomy 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 101150022758 bphA gene Proteins 0.000 description 1
- 101150086954 bphB gene Proteins 0.000 description 1
- 101150005170 bphC gene Proteins 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 101150029472 hsaC gene Proteins 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60124554A JPS61282069A (ja) | 1985-06-08 | 1985-06-08 | ビフェニル代謝機能を備えた形質転換微生物シュウドモナス・エルギノ−サｋｆ２５７株 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60124554A JPS61282069A (ja) | 1985-06-08 | 1985-06-08 | ビフェニル代謝機能を備えた形質転換微生物シュウドモナス・エルギノ−サｋｆ２５７株 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61282069A true JPS61282069A (ja) | 1986-12-12 |
| JPH0336510B2 JPH0336510B2 (cs) | 1991-05-31 |
Family
ID=14888350
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60124554A Granted JPS61282069A (ja) | 1985-06-08 | 1985-06-08 | ビフェニル代謝機能を備えた形質転換微生物シュウドモナス・エルギノ−サｋｆ２５７株 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61282069A (cs) |
-
1985
- 1985-06-08 JP JP60124554A patent/JPS61282069A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0336510B2 (cs) | 1991-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7083953B2 (en) | Method for the production of p-Hydrozybenzoate in species of Pseudomonas and Agrobacterium | |
| JPH03251181A (ja) | インドール酢酸生合成酵素をコードする遺伝子 | |
| US5352599A (en) | Co-enzyme-independent L-sorbosone dehydrogenase of gluconobacter oxydans: isolation, characterization, and cloning and autologus expression of the gene | |
| JP3289726B2 (ja) | クラバラン酸の生合成遺伝子 | |
| JP2577876B2 (ja) | 遺伝子的に修飾された生物体を用いるビタミンｃ先駆体の製造に係る酵素、およびその製造方法 | |
| US5550046A (en) | DNA encoding α-glucosidase and method of producing same by genetic engineering | |
| JPS61282069A (ja) | ビフェニル代謝機能を備えた形質転換微生物シュウドモナス・エルギノ−サｋｆ２５７株 | |
| JPH0740936B2 (ja) | アスコルビン酸の中間体およびプロセス酵素類 | |
| CN116904328A (zh) | 一种高表达啶南平a的工程菌及发酵培养基 | |
| US4861717A (en) | Microorganisms and plasmids for the constitutive formation of creatinamidinohydrolase and processes for the production thereof | |
| JP2960775B2 (ja) | ノカルジオフォーム微生物由来のｄｎａ配列の発現 | |
| JPS61282068A (ja) | ビフエニル代謝機能を備えた形質転換微生物シユウドモナス・プチダｋｆ１３８株 | |
| US5308765A (en) | Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically acitve carboxylic acids and their enantiomeric esters using said transformants | |
| US5306625A (en) | Process for terminal hydroxylation of ethyl groups on aromatic 5- or 6-ring heterocycles | |
| JP2857886B2 (ja) | バチルス・エス・ピー、ｌ―乳酸脱水素酵素遺伝子を含有するｄｎａ断片およびそれを含有する組み換え体プラスミド並びにｌ―乳酸脱水素酵素遺伝子およびそれを含有する組み換え体プラスミド。 | |
| JPS61282085A (ja) | ビフエニル代謝機能を備えた外来遺伝子ｂｐｈ Ａ−Ｃ及びこれを保有する新規な微生物 | |
| US6245524B1 (en) | Phenylacetyl-CoA ligase from penicillium chrysogenum | |
| US6022703A (en) | Expression of DNA sequences derived from nocardioform microorganisms | |
| JP2003088380A (ja) | ビフェニル又はその誘導体の分解法、及びそれに用いられる微生物 | |
| DE3850294T2 (de) | Für A.Nidulans-Isopenicillin-N-Synthetase codierende DNS, deren Verwendung und dafür codierende Vektoren. | |
| JP3330670B2 (ja) | アルケンモノオキシゲナーゼ、これをコードする遺伝子及び形質転換微生物並びにアルケンのエポキシ化方法 | |
| US6037156A (en) | DNA molecules and vectors encoding clavulanic acid biosynthesis enzymes | |
| JPH0928379A (ja) | 環状プラスミド | |
| JPH08103269A (ja) | カルボニルレダクターゼ遺伝子の塩基配列及びその利用法 | |
| CN114480459A (zh) | 氧化酶全细胞催化剂及其制备高光学纯度r型1,3丁二醇的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |