JPS61176518A - Mouth wash - Google Patents

Abstract

PURPOSE:To keep the pH of a mouth wash containing dextranase and a perfume within a specific range, and to suppress the dectivation of the dextranase for a long period, by using a nonionic surfactant as a solubilizing agent for the perfume. CONSTITUTION:A mouth wash containing 100-200,000 unit/ml of dextranase and 0.01-2wt% perfume is compounded with 0.002-0.15mol/l, preferably 0.01-0.1mol/l of a buffering agent and 0.1-7wt% nonionic surfactant (e.g. sorbitan fatty acid ester, polyoxyethylene higher alcohol ether, etc.) as a solubilizing agent for the perfume, to keep the pH of the composition to 5-7, preferably 5.5-6.5. the buffering agent is phthalic acid, phosphoric acid, acetic acid, citric acid, carbonic acid, etc., their salts, amino acids or their salts, ribonucleic acids or their salts, sodium hydroxide, borax, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a mouthwash containing dextranase, and more specifically, the deactivation of dextranase during storage is reliably suppressed, and its effectiveness is The present invention relates to a mouthwash that exhibits good performance over a long period of time.

−That.

Conventionally, it has been known that dextranase is incorporated into oral compositions as an active ingredient for caries prevention in order to suppress the formation of dental plaque.

However, dextranase is easily deactivated by the influence of solvents such as water, and therefore, when dextranase is added to a liquid mouthwash containing a large amount of solvent such as water, dextranase is easily deactivated during storage, especially when stored at high temperatures. Sometimes it loses its activity to a considerable extent,
There is a problem that its effectiveness is not well demonstrated.

Additionally, liquid mouthwashes such as mouthwashes usually contain anionic surfactants as fragrance solubilizers, but there is a problem in that dextranase is easily deactivated by the effects of anionic surfactants. .

1! In view of the above circumstances, the present inventors conducted extensive research into stably blending dextranase into liquid mouthwashes such as mouthwashes, and found that the amount of dextranase in the total amount of 100 to 200,000 units A mw agent is added to a mouthwash containing 0.01 to 2% of the total amount of 711 and fragrance to adjust and maintain the pH in the range of 5 to 7, and a nonionic surfactant is added as a fragrance solubilizer. When the agent is added at 0.01 to 7% by weight of the total, deactivation of dextranase is surprisingly suppressed, dextranase is stably maintained over a long period of time, and oral cavity of good quality is maintained. The present invention was based on the discovery that a cleaning agent can be obtained.

The present invention will be explained in more detail below.

11. The oral cleansing agent of the present invention contains dextranase in a total amount of 10%.
0 to 200,000 units 71! and fragrance to a total of 0.01
A buffering agent of 0.002 to 0.15 mol/J and a nonionic surfactant of 0.01 to 7 mol/J of the total are added to a liquid oral cleaning solution containing ~2% by weight. 7 and is used as a mouthwash, etc.

In this case, the dextranase produced from various dextranase-producing bacteria can be used, for example, those derived from the genus Penicillium, Aspergillus, Chaetomium, Streptomyces, Sporochichum, Bacillus, etc. However, from the viewpoint of dextran-degrading ability, it is most preferable to use one derived from the filamentous fungus Chaetomium.

In addition, as mentioned above, the blending amount of dextranase is 100 to 200,000 units/1 to the entire cleaning agent! It is used as a cleaning agent, but especially in the case of undiluted type, it is 200~
10,000 units/J, 500 to io for diluted type
It is preferable to set it to oooo units/xl. Here, 1 unit means when the reaction is carried out using dextran as a substrate,
Glucose 1 μ per minute? This refers to the amount of enzyme that produces free reducing sugars equivalent to .

In the present invention, the fragrance is menthol.

Carvone, anethole, eugenol, methyl salicylate, limonene, ocimene, n-decyl alcohol, citronellol, α-terpineol.

Methyl acetate, cyto-Oneryl acetate, methyl eugenol, cineole, linalool, ethyl linalool, vanillin, thymol, spearmint oil, vapormint oil, lemon oil, orange oil, sage oil, rosemary oil, cinnamon oil, viment oil, cinnamon oil oil, perilla oil, wintergreen oil9
One or more of clove oil, eucalyptus oil, etc. can be used.

Furthermore, in the present invention, a nonionic surfactant is used as a solubilizer for these fragrances, and examples of the nonionic surfactant include sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, and polyoxyethylene sorbitol fatty acid ester. Ester, polyoxyethylene fatty acid ester, polyoxyethylene higher alcohol ether, polyoxyethylene allyl ether, polyoxyethylene lanolin derivative, polyoxyethylene lanolin alcohol derivative, polyoxyethylene castor oil derivative, polyoxypropylene polyoxyethylene cetyl alcohol ether It is preferable to use one or more selected from polyoxyethylene alkylphenol formaldehyde condensate derivatives, polyoxyethylene alkylamines, and polyoxyethylene alkylamides.

The oral cleansing agent of the present invention includes a buffering agent as described above,
The pH is adjusted and maintained in the range of 5 to 7, and buffering agents include phthalic acid, phosphoric acid, phosphoric acid, succinic acid, acetic acid, fumaric acid, malic acid, carbonic acid, and their sodium and potassium salts. One or more selected from ammonium salts, amino acids and their salts, ribonucleic acids and their salts, sodium hydroxide/borax, hydrochloric acid/bicarbonate, etc. are preferably used. Among these, it is particularly preferable to use neutral amino acids, acidic amino acids, or salts thereof. Here, the neutral amino acids are glycine, alanine, serine, and leucine, and the acidic amino acids are aspartic acid.

Examples include glutamic acid and asparagine glutamine, respectively. In addition, ribonucleic acid and its salts are also preferred, and examples of the ribonucleic acid salts include sodium 5'-inosinate, 5'-sodium inosinate,
′-Sodium uridylate.

Sodium 5'-guanylate and the like are preferably used.

The blending amount of the buffering agent is 0.002 to 0.15 mol/J of the total, preferably 0.01 to 0.1 mol/J, and the blending amount of the buffering agent is within this range. In some cases, the dextranase stabilizing effect is reliably exhibited, but when it is outside this range, the dextranase stabilizing effect is insufficient and the object of the present invention cannot be achieved. In particular, if the buffering agent content is less than 0.002 mol/J, it has no effect on stabilizing -, and if it is more than 0.15 mol/J, the stabilizing effect of - reaches a saturated state, and its solubility Problems arise in terms of the usability of detergents and detergents.

As mentioned above, the state of the cleaning agent of the present invention is in the range of 5 to 7, and by keeping the cleaning agent within this range,
Deactivation of dextranase is effectively prevented. On the other hand, if the detergent is outside the range of 5 to 7, dextranase is likely to be deactivated and dextranase cannot be stably retained, which is not preferable. In addition, the more preferable range of - is 5.5-6.5.

The mouthwash of the present invention is one in which the above-mentioned components are dissolved in a solvent, and examples of the solvent include water, ethyl alcohol, isopropyl alcohol, sorbitol solution, glycerin, ethylene glycol, propylene glycol, etc.
-Ptylene glycol, polyethylene glycol (#4
It is preferable to use one type or a mixture of two or more types of polyhydric alcohols such as 00 to #4000), polypropylene glycol, xylit, maltit, and lactit. The content of water is 0 to 99% by weight, especially 20 to 90% by weight, and the content of ethyl alcohol and isopropyl alcohol is 0 to 6!11% of the total, especially 10% by weight.
~80% heavy yang, the amount of polyhydric alcohol is 0-9 of the total
It is preferably 9% by weight, particularly 10 to 80% by weight. In this case, the detergent of the present invention has the above-described structure, so that dextranase is stably blended in an aqueous system.

Furthermore, as other components of the mouthwash of the present invention, appropriate commonly used components may be blended.

For example, sweeteners (0 to 1% by weight, preferably 0.01 to 0
．． 5% by weight), p- and droxymethylbenzoic acid, tsukuaside, p-hydroxyethylbenzoic acid, p-hydroxypropylbenzoic acid, p-hydroxybutylbenzoic acid, sodium benzoate, lower fatty acid monoglyceride, etc. Preservative.

Gelatin, peptone, arginine hydrochloride, albumin,
Casein, pigments, and other ingredients may be blended.

In addition to dextranase, active ingredients include enzymes such as amylase, protease, mutanase, lysozyme, lytic enzyme, and Lytec enzyme, alkali metal monofluoro-Ω phosphates such as sodium monofluorophosphate and potassium monofluorophosphate, and fluoride. Sodium, fluorides such as stannous fluoride, tranexamic acid and epsilon aminocaproic acid, chlorhexidine hydrochloride, chlorhexidine gluconate, aluminum chlorohydroxyallantoin, dihydrocholesterol, glycyrrhizin, dipotassium glycyrrhizin, glycyrrhetinic acid, glycerophosphate.

Contains one or two active ingredients such as chlorophyll, copper chlorophyllin sodium, sodium chloride, dyrobeptide, water-soluble inorganic phosphoric acid compound, azulene, chamomile, dangki, xun medicine, yobiki shengsan, and gajo. More than one species can be combined.

The cleaning agent of the present invention is applied as it is or after being diluted with water etc. depending on the concentration of its components, but the oral cleaning agent of the present invention reliably suppresses the deactivation of dextranase during storage. Since the effectiveness of dextranase is maintained over a long period of time, the effect of dextranase is effectively exhibited when applied to the oral cavity.

Next, examples and comparative examples will be shown to specifically explain the present invention.

In addition, in the following examples, all percentages indicate weight percent.

[Examples 1-3. Comparative Example] Examples 1 to 3 shown in Table 1. Mouthwashes of Comparative Examples 1 to 5 were prepared, and the residual activity of dextranase after storage at 40° C. for one month was examined. The results are shown in Table 1. The evaluation criteria for the test results are as follows.

O: Residual activity is 70% or more when manufactured by m Δ: 30-70% This was recognized. In contrast, comparative example 1. Comparative example 2 with high state. Comparative Examples 3 and 11 with a small amount of buffering agent; Comparative Example 4 with a large amount of buffering agent. In the mouthwash of Comparative Example 5 using an anionic surfactant, the dextranase was unstable when stored at high temperatures.

The detergents of Examples 1 to 3 had residual dextranase activity of 80% or more after being stored at room temperature for 3 years, and were found to be sufficiently stable even after long-term storage.

[Example 4] Dextranase 0.01% (200
5'-sodium guanylate 0.8 citric acid
0.2 polyoxyethylene (
60) 5 cured 1 better oil fragrance
185% glycerin 10 Sodium benzoate 0.5 Ethyl alcohol
3 Saccharin Sodium
0.2 Water 100.0% Dextranase residual activity of this mouthwash was determined in Example 1.
The result of evaluation in the same manner as 3 was "OII".

Applicant: Laion Co., Ltd. Agent: Patent Attorney Takashi Kojima Procedural amendment export) March 20, 1985 1985 Patent Application No. 174.76 2. Name of the invention mouthwash 3. Person who makes corrections Relationship to the case Patent applicant 6. Contents of amendment (1) Revise the scope of claims as shown in the attached sheet.

(2) Ming 11! Ili! Page 5, lines 8 to 9 r
o, 002 to 0.15 mol/jJ is IQ, 00
2 mol/J or more.''

(3) ro, 002, page 8, lines 17 to 18
〜・・・・・・0.1'' means ro, 002 mol/
J or more, preferably 0.01 to 1.”

(4) On page 9, line 5, 0.15 mol/jJ is corrected to 1 mol/jJ.

(5) On page 9, line 7, the phrase ``problems may occur'' is corrected to ``problems may occur.''

(6) "Examples 1 to 3" on page 12, line 19 and line 20, page 15, line 9, and page 16, line 8, respectively.
" has been corrected to "Examples 1 to 5."

(7) The words "Table 1" on page 12, line 20, page 13, line 3, and page 15, line 1, respectively, are replaced with "Table 1 and Table 2." correct.

(9) On page 15, line 13, “Example 4” was replaced with “
Example 6” is corrected.

Claims [1.
Unit/1! For a liquid mouthwash containing 0.01 to 2% by weight of perfume and fragrance, the buffering agent should be at least 0.002 mol/J of the total and the nonionic surfactant should be 0.01 to 2% by weight of the total.
An oral cavity cleansing agent containing 7% by weight and manufactured with a grade of 5 to 7.

2. Buffers include phthalic acid, phosphorus m, and citric acid.

Succinic acid, acetic acid, fumaric acid, malic acid and carbonic acid and their salts, thorium, potassium and ammonium;
Amino acids and their salts: Ribonucleic acids and their salts: Sodium hydroxide, borax: 1 selected from hydrochloric acid and hydrogen carbonate
The mouthwash according to claim 1, which is one or more types.

3. The mouthwash according to claim 2, wherein the amino acid is a neutral amino acid or an acidic amino acid.

4. The mouthwash according to claim 3, wherein the neutral amino acid is glycine, alanine, serine, or leucine.

5. Claim 3, wherein the acidic amino acid is aspartic acid, glutamic acid, or aspartic glutamine.
Oral cleansing agent as described in section.

6. Ribonucleic acid salts are sodium 5'-inosinate, 5
The mouthwash according to claim 2, which is sodium '-uridylate or sodium 5'-guanylate.

7. The nonionic surfactant is sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitol fatty acid ester, polyoxyethylene fatty acid ester, polyoxyethylene higher alcohol ether, polyoxyethylene allyl ether.

Polyoxyethylene lanolin derivative, polyoxyethylene lanolin alcohol derivative, polyoxyethylene castor oil derivative, polyoxypropylene polyoxyethylene cetyl alcohol ether, polyoxyethylene alkylphenol formaldehyde condensate derivative, polyoxyethylene alkylamine, polyoxyethylene alkyl 1 chosen from Amide! or 2 or more types of oral cavity cleansers according to any one of claims 1 to 6. ”

Claims (1)

[Claims] 1. For a liquid mouthwash containing 100 to 200,000 units/ml of dextranase and 0.01 to 2% by weight of fragrance, the amount of buffering agent is 0.002 to 2% by weight of the total. 0.
15 mol/l and nonionic surfactant at a total content of 0.0
A mouthwash characterized by containing 1 to 7% by weight and adjusting the pH to 5 to 7. 2. Buffers include phthalic acid, phosphoric acid, citric acid, succinic acid, acetic acid, fumaric acid, malic acid and carbonic acid, and their sodium, potassium and ammonium salts: amino acids and their salts; ribonucleic acid and its salts: hydroxylation The mouthwash according to claim 1, which is one or more selected from sodium and borax; hydrochloric acid and hydrogen carbonate. 3. The mouthwash according to claim 2, wherein the amino acid is a neutral amino acid or an acidic amino acid. 4. The mouthwash according to claim 3, wherein the neutral amino acid is glycine, alanine, serine, or leucine. 5. The mouthwash according to claim 3, wherein the acidic amino acid is aspartic acid, glutamic acid, or aspartic glutamine. 6. Ribonucleic acid salts are sodium 5'-inosinate, 5
The mouthwash according to claim 2, which is sodium '-uridylate or sodium 5'-guanylate. 7. The nonionic surfactant is sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitol fatty acid ester, polyoxyethylene fatty acid ester, polyoxyethylene higher alcohol ether, polyoxyethylene allyl ether, polyoxyethylene lanolin derivatives selected from polyoxyethylene lanolin alcohol derivatives, polyoxyethylene castor oil derivatives, polyoxypropylene polyoxyethylene cetyl alcohol ether, polyoxyethylene alkylphenol formaldehyde condensate derivatives, polyoxyethylene alkyl amines, polyoxyethylene alkyl amide The oral cavity cleansing agent according to any one of claims 1 to 6, which is one or more types.