JPS61172031A - Method for measuring combined amount of nitrate nitrogen and nitrite nitrogen - Google Patents
Method for measuring combined amount of nitrate nitrogen and nitrite nitrogenInfo
- Publication number
- JPS61172031A JPS61172031A JP261686A JP261686A JPS61172031A JP S61172031 A JPS61172031 A JP S61172031A JP 261686 A JP261686 A JP 261686A JP 261686 A JP261686 A JP 261686A JP S61172031 A JPS61172031 A JP S61172031A
- Authority
- JP
- Japan
- Prior art keywords
- nitrogen
- measured
- wavelengths
- absorbancies
- difference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 33
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 title claims abstract description 31
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 238000001228 spectrum Methods 0.000 claims abstract description 11
- 238000002835 absorbance Methods 0.000 claims description 38
- 238000000862 absorption spectrum Methods 0.000 claims description 4
- 239000012086 standard solution Substances 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 34
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 17
- 238000005259 measurement Methods 0.000 abstract description 8
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 238000011109 contamination Methods 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000013535 sea water Substances 0.000 description 11
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】
〔発明の技術分野〕
本発明は無機態窒素、更に詳しくは水中における硝酸態
窒素及び亜硝酸態窒素の合計の含有量（以下合量という
）を正しく、かつ自動的に測定する方法に関するもので
ある。[Detailed Description of the Invention] [Technical Field of the Invention] The present invention accurately and automatically measures inorganic nitrogen, more specifically, the total content of nitrate nitrogen and nitrite nitrogen (hereinafter referred to as the total amount) in water. It is related to the method of measuring.
無機態窒素にはアンモニア態窒素、硝酸態窒素及び亜硝
酸態窒素の３態が存在し、これらを廃水に混ぜて放流、
特に内海や湖へ放流すると、水域の赤潮発生の原因とな
る。そこで関係者は無機態窒素含有廃液を流さないよう
努力を続けているが一方では放流に対する監視体制、特
に自動的に水中の無機態窒素の含有量を測定する方法の
確立にも努力が払われている。Inorganic nitrogen exists in three states: ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen, and these are mixed with wastewater and discharged.
In particular, if released into inland seas or lakes, it can cause red tide in the water area. Therefore, those involved are continuing to make efforts to prevent inorganic nitrogen-containing wastewater from being discharged, but efforts are also being made to establish a monitoring system for discharge, especially a method to automatically measure the content of inorganic nitrogen in water. ing.
無機態窒素３態のうち、アンモニア態窒素の含有量につ
いてはイオン選択性電極を使って一応相当の精度をもっ
て測定することができるのでその自動測定はいまやさし
て重要な課題ではないが、硝酸態窒素ど亜硝酸態窒素の
合量を自動測定する方法の確立は重要な課題となってい
る。Among the three forms of inorganic nitrogen, the content of ammonia nitrogen can be measured with considerable accuracy using an ion-selective electrode, so automatic measurement is not an important issue at the moment. Establishing a method to automatically measure the total amount of nitrite is an important issue.
ところが、上記窒素を単独で測定する方法は存在するが
、合量を測定する有効な方法はない。However, although there are methods for measuring nitrogen alone, there is no effective method for measuring the total amount.
例えばこれまで水中の硝酸態窒素の定量法として、アー
ル・エル・ヤングらのポーラログラフを用いる方法、エ
ル・アール・マツケンジーらのイオン選択性電極による
方法、ニス・シャハイネらによる吸光光度法やビー・ケ
ー・アフグハンらによるケイ光光度法があり、また、硝
酸態窒素の紫外部吸収測定法として２１０ｎｍにおける
吸光度測定法、２１０ｎｍにおける吸光度から２７５ｎ
ｍにおける吸光度の４倍を差引く方法、２２０ｎｍにお
ける吸光度から２７５ｎｍにおける吸光度の２．５倍を
差引（方法または２１５ｎｍ 、２２０ｎｍ、２０５ｎ
ｍ。For example, the methods for quantifying nitrate nitrogen in water that have been used so far include a polarographic method by R.L. Young et al., a method using an ion-selective electrode by L.R. Matsukenzie et al., a spectrophotometric method by Nis Shahine et al.・There is a fluorescence photometry method by K. Afghan et al. Also, as a method for measuring ultraviolet absorption of nitrate nitrogen, there is a method of measuring absorbance at 210 nm, and a method of measuring absorbance at 275 nm from the absorbance at 210 nm.
2.5 times the absorbance at 275 nm from the absorbance at 220 nm (method or 215 nm, 220 nm, 205 nm)
m.
２１０ｎｎ＋、 ２２５ｎｎ＋、 ２３０ｎｍにおける
吸光度を測定し、複雑な計算をした後、硝酸態窒素の含
有量を求める方法等がある。There is a method of measuring the absorbance at 210 nn+, 225 nn+, and 230 nm and calculating the nitrate nitrogen content after performing complicated calculations.
また、亜硝酸態窒素のみを測定する方法としては特殊な
有機試薬を使用する吸光光度法による方法がある。しか
しいずれも硝酸態窒素と亜硝酸態窒素の合量を測定する
ことができない。Further, as a method for measuring only nitrite nitrogen, there is a method using a spectrophotometric method using a special organic reagent. However, neither method can measure the total amount of nitrate nitrogen and nitrite nitrogen.
ただ、英国特許第１３０９５５１号明細書にのみ、水中
の有機性炭素や懸濁物などとともに窒素酸化物の全量を
測定する方法が提案されているが、この方法は複雑な特
殊構造の装置を使用しなければならないので簡単に、ど
こでもこの方法を利用して窒素酸化物を測定することは
できない。However, only British Patent No. 1309551 proposes a method for measuring the total amount of nitrogen oxides as well as organic carbon and suspended solids in water, but this method uses a device with a complicated special structure. Because it has to be done easily, nitrogen oxides cannot be measured everywhere using this method.
硝酸態窒素及び亜硝酸態窒素は共に赤潮発生をもたらす
富栄養化源であるから、それらの個個の含有量を測定し
なくても両者の合量としてそれらの含有量を容易に、か
つ正確に測定することができれば、アンモニア態窒素の
測定法と°併用して水中の無機態窒素の測定及び汚染監
視体制の強化が可能となり、工業的または公害防止的な
見地からする極めて意義深い。Since both nitrate nitrogen and nitrite nitrogen are eutrophication sources that cause red tide, it is easy and accurate to calculate their total content without measuring their individual contents. If it can be measured, it will be possible to measure inorganic nitrogen in water and strengthen the pollution monitoring system by using it in conjunction with the method for measuring ammonia nitrogen, which is extremely significant from an industrial or pollution prevention standpoint.
一般に吸光光度分析においては、スペクトルの特定の波
長をいくつか選んで、それらの波長における吸光度を測
定し、計算によって試料中に含まれている数種の各成分
の各含有量を決定する方法が公知である。この方法に対
し、本発明者の意図するところは、態様の異なる２種類
の窒素化合物の合量を正しく測定しようとするものであ
り、その指向する目的に従来と異なる点があると同時に
、むしろ本発明の利用分野を考えれば、発明者の意図は
合理的である。In general, in spectrophotometric analysis, a number of specific wavelengths in the spectrum are selected, the absorbance at those wavelengths is measured, and the content of each of the several components contained in the sample is determined by calculation. It is publicly known. The inventor's intention with this method is to accurately measure the total amount of two types of nitrogen compounds with different embodiments, and at the same time that the intended purpose is different from the conventional method, it is rather Considering the field of application of the present invention, the inventor's intention is reasonable.
そこで、本発明者は硝酸態窒素と亜硝酸態窒素の合量の
測定法について鋭意検討した結果、特定の波長における
吸光度を測定してこれに基づく演算により、上記の合量
を正確に測定し得るとの事実を見い出すに到った。Therefore, the inventors of the present invention have conducted extensive research on methods for measuring the total amount of nitrate nitrogen and nitrite nitrogen, and have determined that the above total amount can be accurately measured by measuring the absorbance at a specific wavelength and performing calculations based on this. I have come to discover the fact that it can be obtained.
従って本発明の目的は硝酸態窒素と亜硝酸態窒素の合量
を正確に測定する方法を確立することにある。Therefore, an object of the present invention is to establish a method for accurately measuring the total amount of nitrate nitrogen and nitrite nitrogen.
すなわち本発明の硝酸態窒素と亜硝酸態窒素の合量を測
定する方法は、硝酸態窒素及び亜硝酸態窒素がそれぞれ
単独に同じ量だけ含まれる二種の標準試料の紫外線吸収
スペクトルＳ１及び３２．の任意の波長λ２、λ３であ
って、前記スペクトルＳ１と３２のそれぞれの吸光度Ｅ
λ１とＥ１λとの差（Ｅλ＋−Ｅｘ工）及びＥ３１とＥ
Ｊユとの差（Ｅ３１−Ｅ３２）が等しくなるような波長
λ２、とλ３における標準溶液の吸光度Ｅ２、Ｅ３を測
定し、一方、被測定試料についても同様に前記波長λ２
とλ３における吸光度Ｅ’２％Ｅ３を測定し、ランバー
ド・ベルの法則に基づきＥ２Ｅ３とＥ’２−Ｅ’３とか
ら被測定試料中の硝酸態窒素と亜硝酸態窒素の合量を算
出することを特徴とするものである。That is, the method of measuring the total amount of nitrate nitrogen and nitrite nitrogen of the present invention is based on the ultraviolet absorption spectra S1 and 32 of two standard samples containing the same amounts of nitrate nitrogen and nitrite nitrogen, respectively. ．． arbitrary wavelengths λ2 and λ3, and the respective absorbances E of the spectra S1 and 32
The difference between λ1 and E1λ (Eλ+-Ex engineering) and E31 and E
Measure the absorbances E2 and E3 of the standard solution at wavelengths λ2 and λ3 such that the difference (E31-E32) from
Measure the absorbance E'2%E3 at It is characterized by this.
具体的に図を用いて本発明法を説明する。まず硝酸態窒
素と亜硝酸態窒素がそれぞれ単独に同じ量、例えば１．
Ｏｐ、ｐ、ｍだけ含まれる二種の人工海水の標準試料を
つくり、対照液として窒素を含まない人工海水を用い１
０．０ｒｖのセル中におけるこれらの紫外線吸光度を測
定しスペクトルを得る。すると第１図のごときスペクト
ルが得られる。即ち曲線Ａが硝酸態窒素の紫外線吸収ス
ペクトルＳ１であり、曲線Ｂが亜硝酸態窒素の紫外線吸
収スペクトルＳ２であり、両スペクトルＳ、と８２とは
波長λ＋ ＝ ２１９ｎｍのところで交錯し、吸光度Ｅ
１＝０．３を示す。他方その波長λ、より大きい波長λ
４のところでも二つのスペクトルＳ１と８２とが交錯し
、その間におけるスペクトルＳ、と３２において、任意
の波長λ２．λ３であって、しかも、その波長λ２とλ
３におけるスペクトルＳ１と８２のそれぞれの吸光度Ｅ
λ１．Ｅ１λとＥ３１ 、Ｅ１２の差（Ｅ、Ｌ＋Ｅｚｚ
）と（Ｅ、、−Ｅ、λ）が等しくなるようなλ２とλ３
が存在する。上記１．０ｐ、ｐ、ｍの試料においてはλ
２ ＝２２３ｎｍ、λ３ ＝２３２ｎｍ、Ｅａｌ ＝０
．２１゜Ｅａｚ ＝０．０７． ＥＪｌ ＝０．２６
． Ｅ７上＝０．１２である。The method of the present invention will be specifically explained using figures. First, nitrate nitrogen and nitrite nitrogen are each individually in the same amount, for example, 1.
Two standard samples of artificial seawater containing only Op, p, and m were prepared, and artificial seawater containing no nitrogen was used as a control solution.
The absorbance of these ultraviolet rays in a 0.0 rv cell is measured to obtain a spectrum. Then, a spectrum as shown in FIG. 1 is obtained. That is, curve A is the ultraviolet absorption spectrum S1 of nitrate nitrogen, curve B is the ultraviolet absorption spectrum S2 of nitrite nitrogen, and both spectra S and 82 intersect at the wavelength λ+ = 219 nm, and the absorbance E
1=0.3 is shown. On the other hand, its wavelength λ, the larger wavelength λ
At point 4, the two spectra S1 and 82 also intersect, and at spectrum S and 32 between them, an arbitrary wavelength λ2. λ3, and its wavelengths λ2 and λ
The respective absorbances E of spectra S1 and 82 at 3
λ1. Difference between E1λ and E31, E12 (E, L+Ezz
) and (E, , -E, λ) are equal.
exists. In the sample of 1.0p, p, m above, λ
2 = 223 nm, λ3 = 232 nm, Eal = 0
．． 21°Eaz =0.07. EJl=0.26
．． E7 upper = 0.12.
本発明においては次に窒素酸化物の含有量を測定せんと
する試料の上記λ２とλ３における試料の吸光度Ｅ２及
びＥ３を測定し、吸光度の差（Ｅ２ Ｅ３）を算出し
、ランバード・ベルの法則を利用して硝酸態窒素と亜硝
酸態窒素の合量を求めるのである。In the present invention, next, the absorbances E2 and E3 of the sample whose nitrogen oxide content is to be measured are measured at the above λ2 and λ3, the difference in absorbance (E2 E3) is calculated, and the Lambard-Bell law is calculated. This is used to find the total amount of nitrate nitrogen and nitrite nitrogen.
かくのどと（すれば硝酸態窒素と亜硝酸態窒素の個々の
含有量を求めてそれらを合量することなく、ただちに水
中の上記二態の窒素の合量を正しく測定することができ
る。If you do this, you can immediately accurately measure the total amount of the above two nitrogen states in water without having to calculate the individual contents of nitrate nitrogen and nitrite nitrogen and add them together.
すなわち、上記λ２とλ３においては、硝酸態窒素と亜
硝酸態窒素のいずれについても、同じ含を量の場合は同
じ吸光度差（Ｅ２−Ｅｘ ）を与えるが、もし別の波長
で測定すれば、両態窒素が異なった吸光度を示すので、
測定値は不正確となる。That is, in the above λ2 and λ3, the same absorbance difference (E2-Ex) will be given for both nitrate nitrogen and nitrite nitrogen if they have the same content, but if measured at different wavelengths, Since both nitrogen states exhibit different absorbances,
Measurements will be inaccurate.
このように、本発明による測定法ではλ２゜λ３のおの
おのが、両態窒素について同じ（Ｅ ２−Ｅ３）を与え
るように選定されるがゆえに、測定の高精度が保証され
るのである。Thus, in the measuring method according to the invention, each of λ2 and λ3 is selected so as to give the same (E 2 -E3) for amphoteric nitrogen, thus ensuring high accuracy of the measurement.
次に実施例をもって本発明の効果を示す。 Next, the effects of the present invention will be illustrated with examples.
実施例１
硝酸態窒素と亜硝酸態窒素の合量が１．０ｐ、ｐ、ｍで
あり、各々の割合が異なる人工海水をつくり、波長２２
３ｎｍと２３２ｎｍにおける吸光度Ｅ２とＥ３を測定し
、その差を算出した結果を第２図の曲線に示した。この
曲線は硝酸態窒素と亜硝酸態窒素の割合が変化しても吸
光度に変化がないこと、すなわち本発明法は正確である
ことを示している。Example 1 Artificial seawater was created in which the total amount of nitrate nitrogen and nitrite nitrogen was 1.0 p, p, m, and the proportions of each were different.
The absorbances E2 and E3 at 3 nm and 232 nm were measured, and the difference between them was calculated, and the results are shown in the curve in FIG. This curve shows that the absorbance does not change even if the ratio of nitrate nitrogen to nitrite nitrogen changes, that is, the method of the present invention is accurate.
この実施例において、測定結果の精度を計算したところ
、変動係数の値で、最小が４．２％、最大で７．５％で
あり、本発明法の精度は高いことがわかった。また、硝
酸態窒素と亜硝酸態窒素の合量が２．０ｐ、ｐ、ｎ＋で
あり、各々の割合が異なる人工海水をつくり、同様の測
定をした結果、測定された吸光度Ｅ２ 、Ｅ３から算出
した吸光度差（Ｅ２ Ｅ３）は合量１．０ｐ、ｐ、ｍ
の場合の正確に２倍であった。同様に計算した精度も変
動係数の値で最小２．１％、最大３．３％であった。In this example, when the accuracy of the measurement results was calculated, the minimum coefficient of variation was 4.2% and the maximum was 7.5%, indicating that the accuracy of the method of the present invention is high. In addition, the total amount of nitrate nitrogen and nitrite nitrogen was 2.0p, p, n+, and artificial seawater with different proportions was made and similar measurements were performed. Calculated from the measured absorbances E2 and E3. The absorbance difference (E2 E3) is a total of 1.0 p, p, m
It was exactly twice as large as in the case of . The precision calculated in the same manner was 2.1% at the minimum and 3.3% at the maximum in terms of the coefficient of variation.
実施例２
硝酸態窒素と亜硝酸態窒素の混合割合がｌ：１であり、
全窒素量、すなわち両態の合量の含有量を種々変化させ
た人工海水を作り、λ２＝２２３ｎｍにおける吸光度Ｅ
２． λ３ ＝ ２３ｇｎｍにおける吸光度Ｅ３を測
定し、吸光度差（Ｅ２ Ｅ３ ）を算出した。その結
果は第３図に示すとおりである。Example 2 The mixing ratio of nitrate nitrogen and nitrite nitrogen was 1:1,
We created artificial seawater with various amounts of total nitrogen, that is, the total content of both nitrogen states, and determined the absorbance E at λ2 = 223 nm.
2. The absorbance E3 at λ3 = 23 gnm was measured, and the absorbance difference (E2 E3 ) was calculated. The results are shown in Figure 3.
これかられかるように人工海水中の全窒素含有量が高く
なれば、直線的に吸光度差（Ｅ２−Ｅ３）が高くなり、
これによって、本発明による測定法−によれば正しく両
態窒素の合量が決定できることがわかる。As we will see, if the total nitrogen content in artificial seawater increases, the absorbance difference (E2-E3) will increase linearly.
This shows that the total amount of amphoteric nitrogen can be accurately determined by the measuring method according to the present invention.
実施例３
硝酸態窒素０．８０ｐ、ｐ、ｍ 、亜硝酸態窒素０．２
０ｐ。Example 3 Nitrate nitrogen 0.80 p, p, m, nitrite nitrogen 0.2
0p.
ｐ、ｍ 、合量１．ＯＯｐ、ｐ、ｍの人工海水に各種の
有機物や無機物を種々の量共存させて、それらと相互作
用があるか否かを検討した。この場合、λ２＝ ２２３
ｎｍにおける吸光度Ｅ２＋ λ３ ＝ ２３２ｎｍに
おける吸光度Ｅ３を測定し、吸光度差（Ｅ２−Ｅ′３）
を算出した。p, m, total amount 1. Various organic and inorganic substances were allowed to coexist in the artificial seawater of OOp, p, and m in various amounts, and whether or not there were interactions with them was investigated. In this case, λ2=223
Absorbance E2 + λ3 = absorbance E3 at 232 nm was measured, and the absorbance difference (E2-E'3)
was calculated.
一方、共存物質を含まない、上記と同一の硝酸態及び亜
硝酸態濃度の人工海水（標準試料）についても同様に吸
光度差（Ｅ２ Ｅ３）を求め、（Ｅ２−Ｅ３ ）と（
Ｅ’２−Ｅ３）とからランバード・ベルの法則によって
硝酸態窒素と亜硝酸態窒素の合量を算出し、その誤差を
求めた。On the other hand, for artificial seawater (standard sample) containing no coexisting substances and having the same nitrate and nitrite concentrations as above, the absorbance difference (E2 E3) was determined in the same way, and (E2-E3) and (
E'2-E3), the total amount of nitrate nitrogen and nitrite nitrogen was calculated using Lambard-Bell's law, and the error thereof was determined.
結果を第１表に示す。The results are shown in Table 1.
（以下余白）
第１表かられかるように、Ｅ２ Ｅ３の値は、比較的
大量の有機物や無機物の共存下で少ない誤差で測定する
ことができ、したがって硝酸態窒素と亜硝酸態窒素の合
量を正しく決定することができる。(Leaving space below) As shown in Table 1, the value of E2 and E3 can be measured with a small error in the coexistence of relatively large amounts of organic and inorganic substances. Able to determine quantities correctly.
実施例４
神戸港湾内の海水中の硝酸態窒素と亜硝酸態窒素の合量
をλ２ ＝ ２２３ｎｍにおける吸光度Ｅ２、λ３ ＝
２３２ｎｍにおける吸光度Ｅ３を測定し、吸光度差（
Ｅ２−Ｅ３）を算出することによって求めた。その場合
１０．０ｎｏｎと５０ 、０ｍ１１１の２種のセルをつ
がい、２回ずつ測定を行なった。一方、標準溶液につい
ても同様に前記三波長における吸光度を測定して吸光度
差を算出し、ランバード・ベルの法則を利用して第２表
の結果を得た。Example 4 The total amount of nitrate nitrogen and nitrite nitrogen in seawater in Kobe Port is determined by absorbance E2 at λ2 = 223 nm, λ3 =
The absorbance E3 at 232 nm was measured, and the absorbance difference (
E2-E3). In this case, two types of cells, 10.0 non, 50, and 0 m111, were paired and measurements were performed twice each. On the other hand, the absorbance of the standard solution at the three wavelengths was similarly measured, the absorbance difference was calculated, and the results shown in Table 2 were obtained using the Lambard-Bell law.
（以下余白）
第２表 海水試料の測定結果
第２表かられかるように、光路長１０．０ｍ−のセルを
用いた場合も、また５０ 、０ｍ＋ｗのセルを用いた場
合も同じ測定値が得られた。また測定した海水中の硝酸
態窒素と亜硝酸態窒素の合量は、約０．３〜０．４ｐ、
ｐ、ｍであることもわかった。(Leaving space below) Table 2 Measurement results for seawater samples As shown in Table 2, the same measured values are obtained when using a cell with an optical path length of 10.0 m- and when using a cell with an optical path length of 50,0 m+w. Obtained. In addition, the total amount of nitrate nitrogen and nitrite nitrogen in the seawater measured was approximately 0.3 to 0.4 p,
It was also found that p and m.
第１図は本発明の詳細な説明するための図面であり、ま
た、第２及び３図は本発明の一実施例の結果を示す図面
である。
第１図
、 第２図
硝酸態および亜硝酸態窒素の含有量（ｐ、ｐ、ｍ）第３
図FIG. 1 is a diagram for explaining the present invention in detail, and FIGS. 2 and 3 are diagrams showing the results of one embodiment of the present invention. Figure 1, Figure 2 Nitrate and nitrite nitrogen content (p, p, m) 3rd
figure
Claims (1)
け含まれる二種の標準試料の紫外線吸収スペクトルＳ＿
１及びＳ＿２の任意の波長λ＿２、λ＿３であって、前
記スペクトルＳ＿１とＳ＿２のそれぞれの吸光度Ｅ＿２
＿１とＥ＿１＿２との差（Ｅ＿２＿１−Ｅ＿２＿２）及
びＥ＿３＿１とＥ＿３＿２との差（Ｅ＿３＿１−Ｅ＿３
＿２）が等しくなるような波長λ＿２とλ＿３における
標準溶液の吸光度Ｅ＿２、Ｅ＿３を測定し、一方、被測
定試料についても同様に前記波長λ＿２とλ＿３におけ
る吸光度Ｅ′＿２、Ｅ′＿３を測定し、ランバード・ベ
ルの法則に基づきＥ＿２−Ｅ＿３とＥ′＿２−Ｅ′＿３
とから被測定試料中の硝酸態窒素と亜硝酸態窒素の合量
を算出することを特徴とする硝酸態窒素と亜硝酸態窒素
の合量を測定する方法。Ultraviolet absorption spectra S_ of two standard samples each containing the same amount of nitrate nitrogen and nitrite nitrogen
1 and S_2 at arbitrary wavelengths λ_2 and λ_3, and the respective absorbances E_2 of the spectra S_1 and S_2.
The difference between _1 and E_1_2 (E_2_1-E_2_2) and the difference between E_3_1 and E_3_2 (E_3_1-E_3
Measure the absorbances E_2 and E_3 of the standard solution at wavelengths λ_2 and λ_3 such that _2) are equal, and measure the absorbances E'_2 and E'_3 of the sample to be measured at the wavelengths λ_2 and λ_3 in the same way, Based on Lambard-Bell's law, E_2-E_3 and E'_2-E'_3
A method for measuring the total amount of nitrate nitrogen and nitrite nitrogen, comprising calculating the total amount of nitrate nitrogen and nitrite nitrogen in a sample to be measured from.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP261686A JPS61172031A (en) | 1986-01-09 | 1986-01-09 | Method for measuring combined amount of nitrate nitrogen and nitrite nitrogen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP261686A JPS61172031A (en) | 1986-01-09 | 1986-01-09 | Method for measuring combined amount of nitrate nitrogen and nitrite nitrogen |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10516376A Division JPS5330379A (en) | 1976-09-01 | 1976-09-01 | Measurement of inorganic form nitrogen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61172031A true JPS61172031A (en) | 1986-08-02 |
| JPS62449B2 JPS62449B2 (en) | 1987-01-08 |
Family
ID=11534334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP261686A Granted JPS61172031A (en) | 1986-01-09 | 1986-01-09 | Method for measuring combined amount of nitrate nitrogen and nitrite nitrogen |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61172031A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63191945A (en) * | 1987-02-05 | 1988-08-09 | Kankyo Eng Kk | Method and apparatus for quantitative determination of non-functional nitrogen |
| JPH0196533A (en) * | 1987-10-07 | 1989-04-14 | Agency Of Ind Science & Technol | Method for measuring total nitrogen by eliminating influence of bromide |
| EP0782202A3 (en) * | 1995-12-27 | 1998-04-22 | Shin-Etsu Handotai Co., Ltd | Compound semiconductor epitaxial wafer |
| DE10228929A1 (en) * | 2002-06-28 | 2004-01-15 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG | Arrangement for measuring the nitrate content in liquids |
| CN104155258A (en) * | 2014-08-15 | 2014-11-19 | 广州衡创测试技术服务有限公司 | Improved determination method for total nitrogen in water |
| JP2019109054A (en) * | 2017-12-15 | 2019-07-04 | 国立大学法人豊橋技術科学大学 | Method and apparatus for detecting concentration of nitrate ion and nitrite ion, and plant growth and prolongation agent manufacturing apparatus |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63130342U (en) * | 1987-02-20 | 1988-08-25 | ||
| JPH048034Y2 (en) * | 1987-09-14 | 1992-03-02 | ||
| JPS6478943A (en) * | 1987-09-21 | 1989-03-24 | Koito Mfg Co Ltd | Lighting fixture for rolling stock |
| JPH01160751A (en) * | 1987-12-17 | 1989-06-23 | Koito Mfg Co Ltd | Vehicle lamp |
| JPH01233130A (en) * | 1988-03-14 | 1989-09-18 | Koito Mfg Co Ltd | Lighting equipment for vehicle |
| JPH01293281A (en) * | 1988-05-20 | 1989-11-27 | Inoue Mtp Co Ltd | Spoiler |
| JPH0314745A (en) * | 1989-06-12 | 1991-01-23 | Koito Mfg Co Ltd | Lighting fixture for car |
-
1986
- 1986-01-09 JP JP261686A patent/JPS61172031A/en active Granted
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63191945A (en) * | 1987-02-05 | 1988-08-09 | Kankyo Eng Kk | Method and apparatus for quantitative determination of non-functional nitrogen |
| JPH0196533A (en) * | 1987-10-07 | 1989-04-14 | Agency Of Ind Science & Technol | Method for measuring total nitrogen by eliminating influence of bromide |
| JPH0579137B2 (en) * | 1987-10-07 | 1993-11-01 | Kogyo Gijutsuin | |
| EP0782202A3 (en) * | 1995-12-27 | 1998-04-22 | Shin-Etsu Handotai Co., Ltd | Compound semiconductor epitaxial wafer |
| DE10228929A1 (en) * | 2002-06-28 | 2004-01-15 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG | Arrangement for measuring the nitrate content in liquids |
| CN104155258A (en) * | 2014-08-15 | 2014-11-19 | 广州衡创测试技术服务有限公司 | Improved determination method for total nitrogen in water |
| JP2019109054A (en) * | 2017-12-15 | 2019-07-04 | 国立大学法人豊橋技術科学大学 | Method and apparatus for detecting concentration of nitrate ion and nitrite ion, and plant growth and prolongation agent manufacturing apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62449B2 (en) | 1987-01-08 |
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