JPS61170397A - モルテイエレラ属糸状菌体の多段抽出処理方法 - Google Patents
モルテイエレラ属糸状菌体の多段抽出処理方法Info
- Publication number
- JPS61170397A JPS61170397A JP1028385A JP1028385A JPS61170397A JP S61170397 A JPS61170397 A JP S61170397A JP 1028385 A JP1028385 A JP 1028385A JP 1028385 A JP1028385 A JP 1028385A JP S61170397 A JPS61170397 A JP S61170397A
- Authority
- JP
- Japan
- Prior art keywords
- extraction
- lipids
- lipid
- solid
- liquid separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000605 extraction Methods 0.000 title claims abstract description 56
- 241000235575 Mortierella Species 0.000 title abstract 3
- 150000002632 lipids Chemical class 0.000 claims abstract description 84
- 239000002904 solvent Substances 0.000 claims abstract description 35
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000000926 separation method Methods 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000004215 Carbon black (E152) Substances 0.000 claims abstract description 15
- 229930195733 hydrocarbon Natural products 0.000 claims abstract description 15
- 150000002430 hydrocarbons Chemical class 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims description 44
- 241000233866 Fungi Species 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 abstract description 43
- 230000007935 neutral effect Effects 0.000 abstract description 25
- 230000000813 microbial effect Effects 0.000 abstract description 4
- 210000003850 cellular structure Anatomy 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 description 14
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 14
- 239000002253 acid Substances 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 238000011084 recovery Methods 0.000 description 7
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 5
- 239000003921 oil Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 229930182558 Sterol Natural products 0.000 description 4
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 4
- 235000003702 sterols Nutrition 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 235000021588 free fatty acids Nutrition 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 150000003432 sterols Chemical class 0.000 description 3
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- 235000021314 Palmitic acid Nutrition 0.000 description 2
- 235000021355 Stearic acid Nutrition 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003925 fat Substances 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 235000020778 linoleic acid Nutrition 0.000 description 2
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012046 mixed solvent Substances 0.000 description 2
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 2
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 2
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- SECPZKHBENQXJG-FPLPWBNLSA-N palmitoleic acid Chemical compound CCCCCC\C=C/CCCCCCCC(O)=O SECPZKHBENQXJG-FPLPWBNLSA-N 0.000 description 2
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 2
- 235000012239 silicon dioxide Nutrition 0.000 description 2
- 239000008117 stearic acid Substances 0.000 description 2
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
- TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 235000021360 Myristic acid Nutrition 0.000 description 1
- TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 1
- 235000021319 Palmitoleic acid Nutrition 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- SECPZKHBENQXJG-UHFFFAOYSA-N cis-palmitoleic acid Natural products CCCCCCC=CCCCCCCCC(O)=O SECPZKHBENQXJG-UHFFFAOYSA-N 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 235000008524 evening primrose extract Nutrition 0.000 description 1
- 229940089020 evening primrose oil Drugs 0.000 description 1
- 239000010475 evening primrose oil Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- -1 sterol ester Chemical class 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1028385A JPS61170397A (ja) | 1985-01-22 | 1985-01-22 | モルテイエレラ属糸状菌体の多段抽出処理方法 |
| US06/905,589 US4870011A (en) | 1985-01-22 | 1985-12-13 | Method for obtaining lipids from fungus bodies |
| PCT/JP1985/000685 WO1986004354A1 (en) | 1985-01-22 | 1985-12-13 | Method for obtaining lipids from fungus bodies |
| DE8686900248T DE3587044T2 (de) | 1985-01-22 | 1985-12-13 | Verfahren zur herstellung von lipiden aus fungusmassen. |
| EP86900248A EP0246324B1 (en) | 1985-01-22 | 1985-12-13 | Method for obtaining lipids from fungus bodies |
| CA000499930A CA1273640A (en) | 1985-01-22 | 1986-01-20 | Method for obtaining lipids from fungus bodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1028385A JPS61170397A (ja) | 1985-01-22 | 1985-01-22 | モルテイエレラ属糸状菌体の多段抽出処理方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61170397A true JPS61170397A (ja) | 1986-08-01 |
| JPS6251595B2 JPS6251595B2 (enrdf_load_stackoverflow) | 1987-10-30 |
Family
ID=11745982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1028385A Granted JPS61170397A (ja) | 1985-01-22 | 1985-01-22 | モルテイエレラ属糸状菌体の多段抽出処理方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61170397A (enrdf_load_stackoverflow) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004504849A (ja) * | 2000-08-02 | 2004-02-19 | デーエスエム・ナムローゼ・フェンノートシャップ | 微生物由来の油の単離方法 |
| US10342772B2 (en) | 2013-12-20 | 2019-07-09 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US10364207B2 (en) | 2013-12-20 | 2019-07-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US10392578B2 (en) | 2010-06-01 | 2019-08-27 | Dsm Ip Assets B.V. | Extraction of lipid from cells and products therefrom |
| US10472316B2 (en) | 2013-12-20 | 2019-11-12 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US11124736B2 (en) | 2013-12-20 | 2021-09-21 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US12104139B2 (en) | 2013-12-20 | 2024-10-01 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
-
1985
- 1985-01-22 JP JP1028385A patent/JPS61170397A/ja active Granted
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004504849A (ja) * | 2000-08-02 | 2004-02-19 | デーエスエム・ナムローゼ・フェンノートシャップ | 微生物由来の油の単離方法 |
| JP2012019794A (ja) * | 2000-08-02 | 2012-02-02 | Dsm Ip Assets Bv | 微生物の細胞から油を生成する方法 |
| JP2014138598A (ja) * | 2000-08-02 | 2014-07-31 | Dsm Ip Assets Bv | 微生物由来の油の単離方法 |
| JP2016208974A (ja) * | 2000-08-02 | 2016-12-15 | ディーエスエム アイピー アセッツ ビー．ブイ． | 微生物由来の油の単離方法 |
| US10392578B2 (en) | 2010-06-01 | 2019-08-27 | Dsm Ip Assets B.V. | Extraction of lipid from cells and products therefrom |
| US10342772B2 (en) | 2013-12-20 | 2019-07-09 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US10364207B2 (en) | 2013-12-20 | 2019-07-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US10472316B2 (en) | 2013-12-20 | 2019-11-12 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US11124736B2 (en) | 2013-12-20 | 2021-09-21 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| US12104139B2 (en) | 2013-12-20 | 2024-10-01 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6251595B2 (enrdf_load_stackoverflow) | 1987-10-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |