JPS61170390A - Enzyme-containing polysaccharide composite - Google Patents

Abstract

PURPOSE:To provide the titled composite composed of a compression-molded polysaccharide containing an intimate mixture of enzyme powder and polysaccharide powder, immobilized by polysaccharide powder, having high stability and applicable to various enzymatic reactions. CONSTITUTION:A mixture of the powder of an enzyme and the powder of a polysaccharide (e.g. cellulose, acetyl cellulose, chitin, etc.) is if necessary adjusted to a proper water-content, and compression-molded (preferably under 100-300kg/cm<2> pressure for 30sec-10min) to obtain the objective composite having a density of preferably 1.1-1.5g/cc. The content of the enzyme in the composite is preferably 4-40wt%.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to the immobilization of enzymes, and more particularly to enzyme-containing polysaccharide complexes immobilized by polysaccharide powder.

Conventionally, various methods have been proposed for enzyme immobilization, including enzyme immobilization methods using adsorption methods, encapsulation methods, covalent bonding methods, crosslinking methods, and the like. but,
Conventional methods include problems such as requiring expensive chemical reagents for immobilization, or immobilization is still insufficient using adsorption methods.

The present invention aims to immobilize enzymes by a simple method, and aims to obtain enzyme immobilized products using polysaccharide powder that is safe for the human body.

According to the present invention, there is provided an enzyme-containing polysaccharide complex consisting of a pressed body of polysaccharide containing an intimate mixture of enzyme and polysaccharide.

As the polysaccharide powder used in the present invention, fine powders of various naturally occurring polysaccharides and their modified products are used. Such polysaccharides are conventionally known substances, such as cellulose and its modified products (acetyl cellulose). , ethyl cellulose, etc.), chitin, chitosan, xylan, etc.

The enzyme-containing polysaccharide complex of the present invention can have various structures, such as a molded body made of an intimate mixture of enzyme powder and polysaccharide powder, and an inner shell made of enzyme powder and polysaccharide powder; Examples include a molded body consisting of an outer shell made of polysaccharide powder formed around the periphery.

The method for producing the enzyme-containing polysaccharide complex of the present invention includes:
A method of adjusting a mixture of enzyme powder and polysaccharide powder to an appropriate moisture content as necessary and press-molding it, or granulating this mixture in advance, embedding the granules in polysaccharide powder, and pressurizing the entire mixture. Method of molding or placing the mixture on top of the polysaccharide powder layer.

There is a method in which a polysaccharide powder layer is further formed on top of the polysaccharide powder layer, and the whole is pressure-molded. In the mixture of enzyme powder and polysaccharide powder, the enzyme content contained in the mixture is 2 to 50% by weight.
, preferably 4 to 40% by weight, and the pressure during molding is:
50-500kg/c+&, preferably 100-300
The density of the enzyme-containing polysaccharide complex of the present invention is usually 1 g.
/cc or more, preferably about 1.1" and 1.5 g/cc.

The enzyme-containing polysaccharide complex of the present invention can be formed into various shapes such as a single disc, a single particle, and a rod.

The enzyme-containing polysaccharide complex of the present invention is easy to produce, and the polysaccharide used as the molding material is highly safe for humans. The enzyme-containing polysaccharide complexes of the invention are applied in one variety of enzymatic reactions in conjunction with the enzyme.

Next, the present invention will be explained in more detail with reference to Examples.

Example 1 Cellulose fine powder (Avicel SF, manufactured by Asahi Kasei Corporation) 20 m
1 g was placed flat in a tablet molding machine (manufactured by Riken Seiki Co., Ltd., Br tablet molding machine for infrared spectrophotometer), and on top of it, 20 mg of cellulose fine powder and 3 mg of β-galactosidase were mixed and stirred well in an agate mortar. Then add 20mg of fine cellulose powder on top of it, making it 200kg/
Pressure was applied for 1 minute at a pressure of c+#, and the diameter was 10+am.

A disc-shaped molded body having a thickness of 0.6+s and a density of 1.27 g/cc was obtained.

Next, this molded body was placed in a 0.4M lactose solution, and the glucose produced was measured. the result.

What is the relative enzymatic activity of immobilized enzyme versus soluble enzyme?

1.8% on the 1st day, 1.7% on the 3rd day, 0.
It was 6%.

Example 2 In Example 1, instead of fine cellulose powder.

A disc-shaped molded body was obtained in the same manner except that chitin fine powder (manufactured by Katakura Chikkarin Co., Ltd.) was used. As a result of measuring the relative enzyme activity of this product, it was 3.7% on the first day and 4.2% on the third day.
%, and 2.1% on the 144th day.

Example 3 A disk-shaped molded body was obtained in the same manner as in Example 1 except that fine chitosan powder (manufactured by Katakura Chikkarin Co., Ltd.) was used instead of fine cellulose powder. The relative enzyme activity of this product was 2.3% on the 1st day, 2.2% on the 3rd day, and 0 on the 144th day.
．． It was 8%.

Claims (1)

[Claims] (1) An enzyme-containing polysaccharide complex consisting of a pressed polysaccharide containing an intimate mixture of enzyme powder and polysaccharide powder.