JPS61111682A - Cultivation apparatus - Google Patents
Cultivation apparatusInfo
- Publication number
- JPS61111682A JPS61111682A JP23313284A JP23313284A JPS61111682A JP S61111682 A JPS61111682 A JP S61111682A JP 23313284 A JP23313284 A JP 23313284A JP 23313284 A JP23313284 A JP 23313284A JP S61111682 A JPS61111682 A JP S61111682A
- Authority
- JP
- Japan
- Prior art keywords
- culture tank
- hollow part
- culture
- driven
- cylindrical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
〔発明の利用分野〕
本発明は一細胞あるいは微生物等の培養に利用さｎる培
養槽を用いた培養装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a culture device using a culture tank used for culturing single cells, microorganisms, etc.
培＃榎を用いた培養装置は、動物細胞、植物細胞の培養
や、微生物等の培養に広々用いられている。例えば、特
開昭５３−６６４８６号公報、特開昭５３−１０４７８
６号公報、特開昭５５−６４７９３号公報、特開昭５６
−１０２７８５号公報、特開昭５７−８７７８号公報、
特開昭５８−１１６６７５号公報、特開昭５９−４５８
７２号公報、特開昭５９−４８０７１号公報には、この
種の培養装置が開示されている。Culture devices using Enoki are widely used for culturing animal cells, plant cells, microorganisms, and the like. For example, JP-A-53-66486, JP-A-53-10478.
Publication No. 6, JP-A-55-64793, JP-A-56
-102785 publication, JP-A-57-8778 publication,
JP-A-58-116675, JP-A-59-458
This type of culture apparatus is disclosed in Japanese Patent Application Publication No. 72 and Japanese Patent Application Laid-open No. 59-48071.
さて、従来のこの種培養装置は、竪型培ａｍが多く使用
されている。また、動物細胞等の培養においては、培養
槽内の培地中の細胞に酸素等のガスを供給しているが、
この供給は気液界面の接触で行なうのが一般的である。Now, in conventional culturing apparatuses for this type of seed, a vertical culture medium is often used. In addition, when culturing animal cells, etc., gases such as oxygen are supplied to the cells in the culture medium in the culture tank.
This supply is generally performed by contacting the gas-liquid interface.
その理由は、培地奢こ蛋白質が含まれており、液中にガ
スをスパージングすると発泡するためである。従って、
竪型の培ＩＦ橙では気液接触面積を大きくとるように設
計さｎる。しかし、槽の容量に反比例して気液接触面積
は小さくなるので十分ではない。この欠点を解決するた
めに横型の培養槽が望まれているが、軸封袋バが槽に２
個所設けられており、無菌状態の保持が難しいという問
題を残しており実用さｎているものは少ない。The reason for this is that the medium contains thick proteins and foams when gas is sparged into the liquid. Therefore,
The vertical culture medium IF orange is designed to have a large air-liquid contact area. However, this is not sufficient because the gas-liquid contact area decreases in inverse proportion to the capacity of the tank. In order to solve this drawback, a horizontal culture tank is desired, but the shaft sealing bag has two parts in the tank.
However, there remains the problem that it is difficult to maintain a sterile state, and there are few practical applications.
本発明の目的は、無菌状態の保持が容易で横型の培養槽
とするに適した培養ＩＪｒｐ！を提供することである。The object of the present invention is to cultivate IJrp which is easy to maintain aseptic conditions and is suitable for use in a horizontal culture tank. The goal is to provide the following.
Ｃ発明のｌｉ要〕
本発明は、培養槽の中央部６二円筒状の中空部を設け、
ｐＩ中空部に駆動マグネ岬トを取付けた駆動軸を通し、
該回転軸を回動自在に取付けると共に、培養槽内であっ
て該中空部を形成する隔壁の内側部には１Ｍ記駆動マグ
ネヴトの回動により駆動さｎる円筒状の被駆動体を設け
、該被駆動体に撹拌翼を取付けたことを特徴とする。C li main points of the invention] The present invention provides a central part 6 of the culture tank with two cylindrical hollow parts,
Pass the drive shaft with the drive magnet cape attached to the pI hollow part,
The rotary shaft is rotatably attached, and a cylindrical driven body is provided inside the partition wall forming the hollow part in the culture tank and is driven by the rotation of the 1M drive magnet, It is characterized in that a stirring blade is attached to the driven body.
以下１本発明を具体的な実施例に基づき詳細に説明する
。ｔＪ１図は木登−の一実施例を示す図であｌｌ−第２
図は第１図の断面図を示している。第１図および＄２図
において、円筒状の培養槽１は横に配置され、横型の培
養槽となっている。この培養槽１の中央部は円筒状の中
空部２を有しており、この中空部には駆動マグネット５
を取付けた駆動軸３は、挿入さｎている。駆動軸３は、
その端部な軸受６により支持されており、中空部内で回
動自在に取付けられている。この駆動軸３は、モータ４
により回転駆動される。培養槽１内であって、中空部２
を形成している隔壁の内９１部には、円筒状の被駆動体
７が設けられ、軸受８によって回動自在に取付けられて
いる。また、被駆動体７には、撹拌翼９が取付けらｎて
いる。この結果、モータ４が駆動軸３を回転駆動させる
ことによって、駆動マグネット５が回転し、この磁力１
こよって被駆動体７が回転し、撹拌翼９が培養槽内で回
転する。ガス供ｌ＆装置工０は、槽１内の細胞等の培養
に必要なガスを供給する。培地供給装置、１１は、細胞
等の培養のための培地および細胞等を供給する。恒温水
替環装Ｒ１２は、培養槽１内の培養温度を最適に保つた
めに潅水を循環させる。デカンタ−１３は、培養槽１内
で培養された細胞等と生成物を含む培地とを分離するた
めのものである。生成物保存ＩＪ！ｌ！！１４は、生成
物を保存するために設けらｎている。The present invention will be explained in detail below based on specific examples. Figure tJ1 is a diagram showing an example of the tree climbing.
The figure shows a sectional view of FIG. In FIGS. 1 and 2, a cylindrical culture tank 1 is placed horizontally, forming a horizontal culture tank. The center of this culture tank 1 has a cylindrical hollow part 2, and a drive magnet 5 is placed in this hollow part.
The drive shaft 3 with the attached is inserted. The drive shaft 3 is
It is supported by a bearing 6 at its end and is rotatably mounted within the hollow part. This drive shaft 3 is connected to a motor 4
Rotationally driven by. Inside the culture tank 1, the hollow part 2
A cylindrical driven body 7 is provided in an inner portion 91 of the partition wall forming the partition wall, and is rotatably mounted by a bearing 8. Further, a stirring blade 9 is attached to the driven body 7. As a result, the motor 4 rotates the drive shaft 3, thereby rotating the drive magnet 5, and this magnetic force 1
As a result, the driven body 7 rotates, and the stirring blades 9 rotate within the culture tank. Gas supply 1 & equipment 0 supplies gas necessary for culturing cells, etc. in tank 1. A medium supply device 11 supplies a medium and cells for culturing cells and the like. The constant temperature water exchange ring R12 circulates irrigation water in order to maintain the culture temperature in the culture tank 1 at an optimum level. The decanter 13 is for separating cells etc. cultured in the culture tank 1 from a medium containing a product. Product preservation IJ! l! ! 14 is provided for storing the product.
上述した培養装置では、培養槽１内を貫通す、る軸貫通
部がなく、槽内を滅菌（例えば蒸気滅菌）後の塀菌状態
の保持が容易である。また、駆動マグネット５は、軸３
の長手方向の周囲に必要個数配置することが可能であ１
１、被駆動体７を回転駆動するための力を大き鳴するこ
とが可能となる。In the above-mentioned culture device, there is no shaft penetrating portion that penetrates the inside of the culture tank 1, and it is easy to maintain the walled state after sterilizing the inside of the tank (for example, steam sterilization). Further, the drive magnet 5 is connected to the shaft 3
It is possible to arrange the necessary number of pieces around the longitudinal direction of 1
1. It becomes possible to produce a large force for rotationally driving the driven body 7.
この結果、被駆動体７の回転駆動力を大きくすることが
要求されろ大容量の培養装置を実現することができる。As a result, it is possible to realize a large-capacity culture apparatus that requires an increase in the rotational driving force of the driven body 7.
そして、この実施例では、培養袋「を横型にしてお１）
、気液接触面積を太き（とることができるので効率が向
上する。In this example, the culture bag was made horizontal (1).
, the gas-liquid contact area can be increased, improving efficiency.
以上説明したように本発明によれば、無菌状態の保持が
容易で横型の培養槽とするに適した培養装置を実現でき
る。As explained above, according to the present invention, it is possible to realize a culture device that can easily maintain a sterile state and is suitable for use as a horizontal culture tank.
第１図は本発明の一実施例を示す図、第２図は第１図に
おける断面図である。FIG. 1 is a diagram showing one embodiment of the present invention, and FIG. 2 is a sectional view of FIG. 1.
Claims (1)
に駆動マグネットを取付けた駆動軸を通し、該駆動軸を
回動自在に取付けると共に、前記培養槽内であって該中
空部を形成する隔壁の内側部には、前記駆動マグネット
の回動により駆動される円筒状の被駆動体を設け、該被
駆動体に撹拌翼を取付けたことを特徴とする培養装置。1. A cylindrical hollow part is provided in the center of the culture tank, a drive shaft with a drive magnet attached thereto is passed through the hollow part, and the drive shaft is rotatably attached. A culture apparatus characterized in that a cylindrical driven body driven by the rotation of the drive magnet is provided on the inner side of the partition wall forming the part, and a stirring blade is attached to the driven body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23313284A JPS61111682A (en) | 1984-11-07 | 1984-11-07 | Cultivation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23313284A JPS61111682A (en) | 1984-11-07 | 1984-11-07 | Cultivation apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS61111682A true JPS61111682A (en) | 1986-05-29 |
Family
ID=16950240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP23313284A Pending JPS61111682A (en) | 1984-11-07 | 1984-11-07 | Cultivation apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61111682A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100398932B1 (en) * | 2000-09-21 | 2003-09-22 | 한국해양연구원 | Bubble―powered zooplankton grazing wheel |
-
1984
- 1984-11-07 JP JP23313284A patent/JPS61111682A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100398932B1 (en) * | 2000-09-21 | 2003-09-22 | 한국해양연구원 | Bubble―powered zooplankton grazing wheel |
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