JPH06261747A - Method for liquid culture of cell and apparatus therefor - Google Patents
Method for liquid culture of cell and apparatus thereforInfo
- Publication number
- JPH06261747A JPH06261747A JP5055415A JP5541593A JPH06261747A JP H06261747 A JPH06261747 A JP H06261747A JP 5055415 A JP5055415 A JP 5055415A JP 5541593 A JP5541593 A JP 5541593A JP H06261747 A JPH06261747 A JP H06261747A
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- Prior art keywords
- culture
- tank
- stirrer
- liquid culture
- liquid
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は生物細胞の培養方法及び
培養装置に係り、特に、微生物フリー条件下で液体懸濁
培養、特に小規模の液体懸濁培養を効率よく行う方法及
びその装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and apparatus for culturing biological cells, and more particularly to a method and apparatus for efficiently carrying out liquid suspension culture, particularly small-scale liquid suspension culture, under microorganism-free conditions. .
【０００２】[0002]
【従来の技術】培養条件の選択や有用成分の探索には通
常、多数の培養系を準備する必要がある。このような場
合、１リットル以上の比較的大規模なスケールでは培養
条件の調節が容易であるが、能率，経済性の点で不利で
あるため、小型スケールの懸濁培養系を多数準備する方
が得策である。培養するには、培養槽に温度、ｐＨ，Ｄ
０のコントロールが必須てなり、その結果、各種の電極
類，ガス給配管，液給配管を設ける必要が生じる。2. Description of the Related Art Generally, it is necessary to prepare a large number of culture systems in order to select culture conditions and search for useful components. In such a case, it is easy to adjust the culture conditions on a relatively large scale of 1 liter or more, but it is disadvantageous in terms of efficiency and economical efficiency. Therefore, it is recommended to prepare a large number of small-scale suspension culture systems. Is a good idea. To culture, add temperature, pH, D
Control of 0 is essential, and as a result, it is necessary to provide various electrodes, gas supply pipes, and liquid supply pipes.
【０００３】しかし、小型スケール、すなわち、張込液
量５００ｍｌ以下の培養槽を用いる場合、電極や配管等
の装着物を側面からと槽上部から挿入する場合が想定さ
れる。しかし張込液量が５００ｍｌの槽の直径はたかだ
か１０cm，３００ｍｌでは７cm以下となるため、側面か
らは端子や配管の液深度が不足しやすく、かつ槽内の構
造を大きく拘束することになる。撹拌方法は(1）特開平
1−95769号公報に記述されているように槽蓋の中心部に
撹拌軸と蓋上部に駆動源を設ける方法が一般的である。
この方法は軸のシール部分や駆動源に槽上面のスペース
の大半を占有されるため、他のセンサや配管を配置でき
なくなるうらみがあった。代案として、(2）黒田行昭編
集，組織培養の技法，ｐ２５０〜２５３（１９８４
年），ニューサイエンス社，(3）並びに特開平4−26277
9号(公開１９９２年９月１８日）や(4）特開平4−27807
6号（公開１９９２年１０月２日）に示されているよう
に、永久磁石片を槽底面に接触させた状態で外部磁力に
より回転して液を流動させる方式も知られている。しか
し、回転子が摺動するため、生物細胞、特に脆弱な動物
細胞や糸状菌の培養では細胞が損傷を受け、効果的な培
養が困難である。中には、(5）村上浩紀編集，細胞制御
工学，ｐ５９〜６９，ｐ７７〜８４（１９８６年）、学
窓社に記述されているように槽上面から棒で回転子を吊
り、槽底部の外部磁力により中心部で回転させるか、円
錐振動運動させる方法も知られているが、同じく槽上面
を占有される欠点を有する。However, in the case of using a small scale, that is, a culture tank having an amount of the infusion liquid of 500 ml or less, it is assumed that the attachments such as electrodes and pipes are inserted from the side and from the top of the tank. However, since the diameter of the tank with 500 ml of the filling liquid is at most 10 cm and less than 7 cm with 300 ml, the depth of the liquid of the terminals and piping is apt to be insufficient from the side, and the structure inside the tank is greatly restricted. The stirring method is (1)
As described in Japanese Patent Laid-Open No. 1-95769, it is common to provide a stirring shaft at the center of the tank lid and a drive source at the top of the lid.
In this method, most of the space on the upper surface of the tank is occupied by the shaft seal portion and the drive source, so that there is a caveat that other sensors and piping cannot be arranged. As an alternative, (2) Edited by Yukiaki Kuroda, Tissue culture technique, p250-253 (1984)
Year), New Science Co., Ltd., (3) and JP-A-4-26277
No. 9 (published September 18, 1992) and (4) JP-A-4-27807
As disclosed in No. 6 (published on October 2, 1992), a method of rotating a liquid by rotating it with an external magnetic force while keeping a permanent magnet piece in contact with the bottom of the tank is also known. However, since the rotor slides, the cells are damaged in the culture of biological cells, particularly fragile animal cells and filamentous fungi, and effective culture is difficult. Inside, (5) Hiroki Murakami, Cell control engineering, p59-69, p77-84 (1986), hanging the rotor with a rod from the top of the tank as described in Gakumadosha, outside the bottom of the tank. There is also known a method of rotating at the center or conically vibrating by magnetic force, but it also has a drawback that the upper surface of the tank is occupied.
【０００４】[0004]
【発明が解決しようとする課題】本発明の目的は、微生
物フリー条件下で生物細胞を小規模で培養するため、槽
上部を占有せずかつ液面下での摺動面を有しない撹拌機
構で液を流動させて培養する方法及び培養装置を提供す
ることにある。DISCLOSURE OF THE INVENTION An object of the present invention is to cultivate biological cells on a small scale under microbial-free conditions, so that the stirring mechanism does not occupy the upper part of the tank and does not have a sliding surface below the liquid surface. It is intended to provide a method and a culture device for culturing a liquid by flowing the liquid.
【０００５】[0005]
【課題を解決するための手段】本発明者は上述の課題を
解決するため、鋭意検討した結果、本発明に到達した。The present inventor has arrived at the present invention as a result of extensive studies in order to solve the above-mentioned problems.
【０００６】本発明の第一の特徴は、浮子，撹拌翼，磁
性体を有する撹拌子を用い、浮子の浮力により培養槽内
の培養液中に懸垂し、培養槽底部の外部磁力により回転
させることにより培養する方法及び該撹拌子を有する培
養装置である。The first feature of the present invention is to use a stirrer, a stirring blade, and a stirrer having a magnetic material, suspend the suspension in the culture solution in the culture tank by the buoyancy of the float, and rotate it by an external magnetic force at the bottom of the culture tank. And a culture device having the stirring bar.
【０００７】第二の特徴は、撹拌子及び槽底外部の各両
極が実質的に同一垂直線上にあることである。The second characteristic is that both the stirrer and each electrode outside the bottom of the vessel are substantially on the same vertical line.
【０００８】第三の特徴は撹拌子の磁性体の位置を液量
に応じて調節することができることである。The third feature is that the position of the magnetic body of the stirrer can be adjusted according to the amount of liquid.
【０００９】第四の特徴は撹拌子の浮子の部分の容積が
下から上に向って増加する構造を有する浮子をもつこと
である。A fourth feature is that the stirrer has a float having a structure in which the volume of the float portion increases from bottom to top.
【００１０】本培養方法及び装置は生物細胞全般に適用
可能である。特に脆弱な細胞である動物細胞に適してい
る。The present culture method and apparatus can be applied to all biological cells. It is particularly suitable for animal cells, which are fragile cells.
【００１１】[0011]
【作用】上述の特徴をそなえることにより、槽上部にあ
る蓋の中心部分を占有することなく、かつ液面下で摺動
部分なしに、液面及び液中の中央部分のみを用い槽内液
を効果的に撹拌できる。したがって、容量の小さい槽で
も各種のセンサや配管の出入口を大容量槽の蓋部に比べ
ても遜色なく設置することができ、かつ液中に懸垂する
生物細胞を摺動部分により損傷することなく液を効果的
に撹拌して円滑な培養を行うことができる。By virtue of having the above-mentioned characteristics, the liquid in the tank can be used without occupying the central part of the lid at the upper part of the tank and without sliding parts below the liquid surface, using only the liquid surface and the central part in the liquid. Can be effectively stirred. Therefore, even in a small-capacity tank, various sensors and inlets and outlets of pipes can be installed in comparison with the lid of a large-capacity tank, and biological cells suspended in the liquid are not damaged by sliding parts. The liquid can be effectively stirred to carry out smooth culture.
【００１２】撹拌子の磁性体と槽外の磁性体とを水平に
配置しても、磁力による撹拌子への引力を精密に調整す
れば回転できるが、外乱等により撹拌子がもみすり運動
をおこし、槽壁や槽内構造物と接触しやすい。これに対
し、両磁性体を縦方向に配列することにより、より円滑
な回転で長期間安定した回転を得ることができる。仕込
液面に応じ培養に先だち磁性体を撹拌子の内部から引き
出すか打ち込んで、槽外磁性体との距離を調節すること
も可能である。Even if the magnetic material of the stirrer and the magnetic material outside the tank are arranged horizontally, they can be rotated by precisely adjusting the attractive force to the stirrer due to the magnetic force, but the stirrer can undergo a rubbing motion due to disturbance or the like. It easily rises and easily contacts the tank wall and internal structures. On the other hand, by arranging both magnetic bodies in the vertical direction, it is possible to obtain smooth rotation and stable rotation for a long period of time. It is also possible to adjust the distance from the magnetic substance outside the tank by pulling out or driving the magnetic substance from the inside of the stirrer prior to culturing depending on the surface of the charged liquid.
【００１３】[0013]
【実施例】さらに本発明の実施例を示し、さらに詳しく
説明する。EXAMPLES Further examples of the present invention will be shown and described in more detail.
【００１４】〈実施例１〉培養槽は槽部分１に蓋部分２
が弾性パッキン３により気密性を保持している。槽部分
１の外壁は槽外の支持１４により支持されたヒータ１２
と接触している。温度センサ１８により槽内の温度を感
知し、温度制御部１３により定値制御される。槽外底中
心部に近接して永久磁石８を配置し、さらに磁石を回転
調節器と連動するモータ１０に接続している。槽の内周
近傍には酸素の溶解手段として多孔性ポリテトラフルオ
ロエチレン製の酸素透過性膜管１９をらせん状に収納し
て、図中には省略されているが、一端から他端に向け酸
素含有ガスを通じるようになっている。培養液２４のｐ
Ｈ及び溶存酸素濃度はそれぞれパッキン１５を介して蓋
部３に付設されたｐＨセンサ１６及びＤ０センサ１７に
より検知される。回転子４は浮子５，撹拌翼６，磁性体
７から構成される。磁性体７は槽底外の磁性体８と同一
垂線上に配置されている。磁性体８はプラスチック壁２
０に包接されている。浮子５の下部の管状構造部分２１
にはバラスト９を適宜充填して浮力を調節できる。さら
に、磁性体７を管状構造部分２１に挿入もしくは引き出
すことによっても、磁性体下端の垂直位置を調節するこ
とができる。この他、図中に部分的に記載もしくは省略
してあるが、必要に応じ、蓋部分２を貫通し、液の入口
２３，液の出口，気相部の圧バランス用の配管２２等も
付設することができる。 〈実施例２〉図２に、本発明に係る撹拌子の一例を示
す。浮子５は球状を呈し、撹拌翼６は浮子５の直径に等
しく下方に伸長し、翼下端中央に磁性体７を配置してい
る。Example 1 The culture tank has a tank portion 1 and a lid portion 2
The airtightness is maintained by the elastic packing 3. The outer wall of the tank portion 1 is a heater 12 supported by a support 14 outside the tank.
Is in contact with. The temperature sensor 18 senses the temperature inside the tank, and the temperature controller 13 controls the temperature to a constant value. A permanent magnet 8 is arranged close to the center of the outer bottom of the tank, and the magnet is connected to a motor 10 that works in conjunction with a rotation adjuster. A porous polytetrafluoroethylene oxygen permeable membrane tube 19 is spirally housed in the vicinity of the inner circumference of the tank as a means for dissolving oxygen, and although not shown in the drawing, it is directed from one end to the other end. It is designed to pass through an oxygen-containing gas. P of culture medium 24
The H and dissolved oxygen concentrations are detected by the pH sensor 16 and the D0 sensor 17 attached to the lid 3 via the packing 15, respectively. The rotor 4 includes a float 5, a stirring blade 6, and a magnetic body 7. The magnetic body 7 is arranged on the same vertical line as the magnetic body 8 outside the bottom of the tank. The magnetic body 8 is the plastic wall 2
It is included in 0. Tubular structure portion 21 under the float 5
The buoyancy can be adjusted by filling the ballast 9 appropriately. Further, the vertical position of the lower end of the magnetic body can be adjusted by inserting or pulling out the magnetic body 7 into or from the tubular structure portion 21. In addition, although partially shown or omitted in the drawing, if necessary, a lid portion 2 is penetrated, and a liquid inlet 23, a liquid outlet, a pipe 22 for pressure balance in the vapor phase portion, etc. are also attached. can do. <Embodiment 2> FIG. 2 shows an example of a stirrer according to the present invention. The float 5 has a spherical shape, the stirring blade 6 extends downwardly by the same diameter as the float 5, and the magnetic body 7 is arranged at the center of the lower end of the blade.
【００１５】〈実施例３〉図１の構造を有する内容積８
００ｍｌ（有効容積５００ｍｌ）の培養槽をあらかじめ
殺菌しておき、これに４５０ｍｌの１０％（Ｖ／Ｖ）牛
血清添加ダルベコ変法イーグルＭＥＭ培地と、種培養液
として５０ｍｌのマウス−マウスハイブリドーマＳＴＫ
−１株の懸濁培養液を無菌的に入れ、初発細胞濃度１×
１０6cells／ｍｌで培養を開始した。培養期間中、温度
を３７℃に維持すると共に、多孔質テフロン製中空糸膜
に５％（Ｖ／Ｖ）炭酸ガス添加空気を通気し、撹拌子の
回転速度を４０rpm に調節し、ｐＨを６.５〜７.２，Ｄ
０を１.０〜１.２ppm に維持した。７日後の生細胞濃度
は１.１×１０7cells／ｍｌ に達した。培養期間中の平
均の生存率は９２％であった。本培養は死細胞が少なく
かつ高い細胞濃度に到達したことを示している。<Embodiment 3> Internal volume 8 having the structure of FIG.
A culture tank of 00 ml (effective volume 500 ml) was sterilized in advance, and 450 ml of 10% (V / V) bovine serum-added Dulbecco's modified Eagle MEM medium and 50 ml of mouse-mouse hybridoma STK as a seed culture medium were added thereto.
-1 strain suspension culture is aseptically added, and initial cell concentration is 1 ×
Culture was started at 10 6 cells / ml. During the culture period, the temperature was maintained at 37 ° C., 5% (V / V) carbon dioxide gas-added air was passed through the hollow fiber membrane made of Teflon, the rotation speed of the stirring bar was adjusted to 40 rpm, and the pH was adjusted to 6 .5-7.2, D
0 was maintained at 1.0-1.2 ppm. The viable cell concentration after 7 days reached 1.1 × 10 7 cells / ml. The average survival rate during the culture period was 92%. The main culture shows that there are few dead cells and a high cell concentration is reached.
【００１６】〈比較例１〉実施例１で用いた培養槽の撹
拌子を取り除き、代りに槽の蓋中心を貫通するメカニカ
ルシール付の撹拌軸の先端に直径５０mmのパドル形撹拌
翼を配置し、蓋上部のモータにより４０rpm で撹拌して
実施例１と同一種の細胞を同一培地で培養した。Ｄ０セ
ンサ及びｐＨセンサは蓋上部のスペースがないため取り
はずしてＤ０及びｐＨとも無調節で行った。その結果、
ｐＨは６.２〜７.５，Ｄ０は0.5〜３.５ の範囲で変動
した。７日後の生細胞濃度は５.１×１０6cells／ｍｌ
にとどまった。生存率は８８％であった。Comparative Example 1 The stirring bar of the culture tank used in Example 1 was removed, and a paddle type stirring blade having a diameter of 50 mm was placed at the tip of a stirring shaft with a mechanical seal that penetrates through the center of the lid of the tank instead. The cells of the same species as in Example 1 were cultivated in the same medium by stirring at 40 rpm with the motor on the top of the lid. Since there was no space above the lid, the D0 sensor and pH sensor were removed, and both D0 and pH were adjusted. as a result,
The pH ranged from 6.2 to 7.5 and the D0 ranged from 0.5 to 3.5. Live cell concentration after 7 days was 5.1 × 10 6 cells / ml
Stayed in. The survival rate was 88%.
【００１７】〈比較例２〉実施例１で用いた培養槽のう
ち、浮子付き撹拌子を取り除き、代りに長さ４５mmのポ
リテトラエチレン被覆永久磁石を回転子として槽底に配
置し、槽底を摺動しながら４０rpm で回転しつつ、実施
例１と同一細胞と同一培地で培養した。Ｄ０センサ及び
ｐＨセンサは実施例１と同じに装着し、ｐＨ及びＤ０を
それぞれ６.５〜７.３，１.０〜１.２ppmに調節した。
７日後の生細胞濃度は４.９×106cells／ｍｌ にとどま
り、生存率は７９％であった。<Comparative Example 2> In the culture tank used in Example 1, the stirrer with a float was removed, and instead a polytetraethylene-coated permanent magnet having a length of 45 mm was placed as a rotor at the bottom of the tank. While rotating the slide at 40 rpm, the same cells and the same medium as in Example 1 were cultured. The D0 sensor and the pH sensor were mounted in the same manner as in Example 1, and the pH and D0 were adjusted to 6.5 to 7.3, 1.0 to 1.2 ppm, respectively.
The viable cell concentration after 7 days remained at 4.9 × 10 6 cells / ml, and the survival rate was 79%.
【００１８】[0018]
【発明の効果】従来の小型槽のように槽上部の中央部分
を撹拌手段が占有しないため、培養槽上部にセンサ類や
各種の配管出入口等を配置できる。このため大型槽と同
じ精度で精密培養が可能になる。また、従来型の一種で
ある槽底で支持した磁石回転子を回転する方式とことな
り、摺動面がないため細胞の損傷がない。Since the stirring means does not occupy the central portion of the upper part of the tank unlike the conventional small-sized tank, it is possible to arrange sensors and various pipe inlets and outlets on the upper part of the culture tank. Therefore, precision culture can be performed with the same accuracy as a large tank. Also, unlike the conventional method of rotating the magnet rotor supported on the bottom of the tank, there is no sliding surface, so there is no damage to cells.
【図１】本発明の一実施例たる培養装置を示す断面図。FIG. 1 is a cross-sectional view showing a culture device as an embodiment of the present invention.
【図２】本発明の一実施例に係る培養装置を構成する撹
拌子を示す説明図。FIG. 2 is an explanatory view showing a stirrer that constitutes a culture device according to an embodiment of the present invention.
１…培養槽部分、２…蓋部分、４…撹拌子、５…浮子、
６…撹拌翼、７…磁性体、８…永久磁石、９…バラス
ト、１０…モータ、１２…ヒータ、１６…ｐＨセンサ、
１７…Ｄ０センサ、１９…酸素透過性中空糸膜、２４…
培養液。1 ... Culture tank part, 2 ... Lid part, 4 ... Stirrer, 5 ... Float,
6 ... Stirrer, 7 ... Magnetic material, 8 ... Permanent magnet, 9 ... Ballast, 10 ... Motor, 12 ... Heater, 16 ... pH sensor,
17 ... D0 sensor, 19 ... Oxygen permeable hollow fiber membrane, 24 ...
Culture fluid.
Claims (10)
て、磁性体と、撹拌翼を有する撹拌子を浮力により培養
液自由表面から懸垂し、培養槽底部の外部磁力により回
転させることにより、前記培養槽内の前記培養液を流動
させることを特徴とする細胞の液体培養方法。1. A liquid culture method using an electromagnetic stirring mechanism, wherein a magnetic material and a stirring bar having a stirring blade are suspended from the free surface of the culture solution by buoyancy and rotated by an external magnetic force at the bottom of the culture tank, whereby the culture is performed. A method for liquid culture of cells, characterized in that the culture medium in the tank is caused to flow.
前記磁性体の両極及び槽底外部に対峙する回転磁性体の
両極を結ぶ線が実質的に同一の垂直線上にある細胞の液
体培養方法。2. The cell according to claim 1, wherein a line connecting the two poles of the magnetic body forming the stirring bar and the two poles of the rotating magnetic body facing the outside of the bottom of the tank is on substantially the same vertical line. Liquid culture method.
する浮子部分の体積が下部から上部の方向に正の勾配で
増加する浮子部分を有する撹拌子を用いる細胞の液体培
養方法。3. The method for liquid culture of cells according to claim 1, wherein the stirrer having a float part in which the volume of the float part constituting the stirrer increases in a positive gradient from the lower part to the upper part.
水面以下に位置する部分に撹拌子の垂直軸から放射状に
撹拌翼を派生する撹拌子を用いる細胞の液体培養方法。4. The liquid culturing method for cells according to claim 1, wherein a stirrer radially deriving a stirring blade from a vertical axis of the stirrer is used in a portion located below the water surface of the stirrer.
子の磁性体の位置を液深に応じて調節する細胞の液体培
養方法。5. The liquid culture method for cells according to claim 1, 2, 3 or 4, wherein the position of the magnetic body of the stirring bar is adjusted according to the liquid depth.
流動させる細胞を培養する装置に於いて、浮子，撹拌
翼，磁性体から構成しかつ槽壁から支持されない撹拌子
を有することを特徴とする細胞の液体培養装置。6. An apparatus for culturing cells in which a stirrer is rotated by electromagnetic stirring to flow a culture solution, which has a stirrer composed of a float, a stirring blade, and a magnetic body and not supported from a tank wall. A liquid culture apparatus for cells.
及び槽底外部に対峙する回転永久磁石の両極とを同一垂
線上に配置する細胞の液体培養装置。7. The liquid culture apparatus for cells according to claim 6, wherein both poles of the magnetic substance of the stirring bar and both poles of the rotating permanent magnet facing the outside of the bottom of the tank are arranged on the same perpendicular line.
積が下から上に向って増加する浮子を有する撹拌子から
なる細胞の液体培養方法。8. The liquid culture method for cells according to claim 6 or 7, comprising a stirrer having a float whose volume of the float increases from bottom to top.
垂直軸から放射状に撹拌翼が延長する撹拌子からなる細
胞の液体培養方法。9. The method for liquid culture of cells according to claim 6, 7 or 8, wherein the stirring bar has a stirring blade extending radially from the vertical axis of the stirring bar.
拌子の磁性体の垂直方向の位置を撹拌子中に比重調整材
を封入することにより調節する細胞の液体培養装置。10. The liquid culture device for cells according to claim 6, 7, 8 or 9, wherein the vertical position of the magnetic material of the stirring bar is adjusted by enclosing a specific gravity adjusting material in the stirring bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5055415A JPH06261747A (en) | 1993-03-16 | 1993-03-16 | Method for liquid culture of cell and apparatus therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5055415A JPH06261747A (en) | 1993-03-16 | 1993-03-16 | Method for liquid culture of cell and apparatus therefor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06261747A true JPH06261747A (en) | 1994-09-20 |
Family
ID=12997944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5055415A Pending JPH06261747A (en) | 1993-03-16 | 1993-03-16 | Method for liquid culture of cell and apparatus therefor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06261747A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1188474A1 (en) * | 2000-09-13 | 2002-03-20 | Levitronix LLC | Magnetic stirrer |
| US6536938B2 (en) | 2000-05-18 | 2003-03-25 | Lei Zhou | Microorganism culture apparatus and method and microorganism culture system using such apparatus |
| US6733171B2 (en) | 2000-09-13 | 2004-05-11 | Levitronix Llc | Magnetic stirring apparatus and an agitating device |
| WO2023032262A1 (en) * | 2021-08-31 | 2023-03-09 | 株式会社島津製作所 | Cell culture apparatus |
-
1993
- 1993-03-16 JP JP5055415A patent/JPH06261747A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6536938B2 (en) | 2000-05-18 | 2003-03-25 | Lei Zhou | Microorganism culture apparatus and method and microorganism culture system using such apparatus |
| EP1188474A1 (en) * | 2000-09-13 | 2002-03-20 | Levitronix LLC | Magnetic stirrer |
| US6733171B2 (en) | 2000-09-13 | 2004-05-11 | Levitronix Llc | Magnetic stirring apparatus and an agitating device |
| WO2023032262A1 (en) * | 2021-08-31 | 2023-03-09 | 株式会社島津製作所 | Cell culture apparatus |
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