JPS6049792A - 微生物の流加培養法 - Google Patents
微生物の流加培養法Info
- Publication number
- JPS6049792A JPS6049792A JP15752683A JP15752683A JPS6049792A JP S6049792 A JPS6049792 A JP S6049792A JP 15752683 A JP15752683 A JP 15752683A JP 15752683 A JP15752683 A JP 15752683A JP S6049792 A JPS6049792 A JP S6049792A
- Authority
- JP
- Japan
- Prior art keywords
- amount
- rate
- dissolved oxygen
- fed
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000005700 microbiome Species 0.000 title claims description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000001301 oxygen Substances 0.000 claims abstract description 38
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 38
- 235000015097 nutrients Nutrition 0.000 claims description 20
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 238000012136 culture method Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 2
- 235000011187 glycerol Nutrition 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 claims 1
- 230000000050 nutritive effect Effects 0.000 abstract 6
- 239000000126 substance Substances 0.000 abstract 3
- 230000007423 decrease Effects 0.000 description 11
- 239000002609 medium Substances 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- AANLCWYVVNBGEE-IDIVVRGQSA-L Disodium inosinate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(NC=NC2=O)=C2N=C1 AANLCWYVVNBGEE-IDIVVRGQSA-L 0.000 description 3
- -1 carbohydrate compounds Chemical class 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000013890 disodium inosinate Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 229920006395 saturated elastomer Polymers 0.000 description 3
- 229930024421 Adenine Natural products 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241001467578 Microbacterium Species 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- SGFRUQZYZBPQCJ-UHFFFAOYSA-N 1h-pyrazolo[3,4-c]pyridin-7-amine Chemical compound NC1=NC=CC2=C1NN=C2 SGFRUQZYZBPQCJ-UHFFFAOYSA-N 0.000 description 1
- JJTNLWSCFYERCK-UHFFFAOYSA-N 7h-pyrrolo[2,3-d]pyrimidine Chemical compound N1=CN=C2NC=CC2=C1 JJTNLWSCFYERCK-UHFFFAOYSA-N 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229950006790 adenosine phosphate Drugs 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 229910000361 cobalt sulfate Inorganic materials 0.000 description 1
- 229940044175 cobalt sulfate Drugs 0.000 description 1
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Substances C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 1
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 235000011147 magnesium chloride Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 229940099607 manganese chloride Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- DSKLYHDHQJANOE-UHFFFAOYSA-N pyrazolo[4,3-d]pyrimidine Chemical compound C1=NC=N[C]2C=NN=C21 DSKLYHDHQJANOE-UHFFFAOYSA-N 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 150000008223 ribosides Chemical class 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15752683A JPS6049792A (ja) | 1983-08-29 | 1983-08-29 | 微生物の流加培養法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15752683A JPS6049792A (ja) | 1983-08-29 | 1983-08-29 | 微生物の流加培養法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6049792A true JPS6049792A (ja) | 1985-03-19 |
| JPH0361439B2 JPH0361439B2 (esLanguage) | 1991-09-19 |
Family
ID=15651591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15752683A Granted JPS6049792A (ja) | 1983-08-29 | 1983-08-29 | 微生物の流加培養法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6049792A (esLanguage) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0249582A (ja) * | 1988-05-20 | 1990-02-19 | Kanegafuchi Chem Ind Co Ltd | 菌体内のリパーゼ活性を高める培養方法 |
| JPH0488320U (esLanguage) * | 1990-12-18 | 1992-07-31 | ||
| JP2012519474A (ja) * | 2009-03-05 | 2012-08-30 | バイオシルタ オサケユキテュア | 液体培養における酵素に基づく流加回分培養技術 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4950989A (esLanguage) * | 1972-05-10 | 1974-05-17 |
-
1983
- 1983-08-29 JP JP15752683A patent/JPS6049792A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4950989A (esLanguage) * | 1972-05-10 | 1974-05-17 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0249582A (ja) * | 1988-05-20 | 1990-02-19 | Kanegafuchi Chem Ind Co Ltd | 菌体内のリパーゼ活性を高める培養方法 |
| JPH0488320U (esLanguage) * | 1990-12-18 | 1992-07-31 | ||
| JP2012519474A (ja) * | 2009-03-05 | 2012-08-30 | バイオシルタ オサケユキテュア | 液体培養における酵素に基づく流加回分培養技術 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0361439B2 (esLanguage) | 1991-09-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bauer et al. | Maximal exponential growth rate and yield of E. coli obtainable in a bench‐scale fermentor | |
| US5912113A (en) | Method and apparatus for controlling carbon source concentration in aerobic cultivation of a microorganism | |
| Ljunggren et al. | Glutamine limited fed-batch culture reduces the overflow metabolism of amino acids in myeloma cells | |
| CN104160016A (zh) | 高密度培养大肠杆菌细胞的方法 | |
| Kleman et al. | Glucose-stat, a glucose-controlled continuous culture | |
| Yang | Optimization of a cultivation process for recombinant protein production by Escherichia coli | |
| JPS6049792A (ja) | 微生物の流加培養法 | |
| Win et al. | Growth kinetics of Saccharomyces cerevisiae in batch and fedbatch cultivation using sugarcane molasses and glucose syrup from cassava starch | |
| Calam et al. | Microbial aspects of fermentation process development | |
| CN101268179A (zh) | 连续培养产醇细菌的装置和培养该细菌的方法 | |
| JP2932791B2 (ja) | 微生物好気培養における炭素源濃度の制御方法及び装置 | |
| Oh et al. | Interactive dual control of glucose and glutamine feeding in hybridoma cultivation | |
| HUT65613A (en) | Process for high cell density fermentation of escherichia coli in an agitated boiler fermenter | |
| Miura et al. | Assimilation of liquid hydrocarbon by microorganisms. II. Growth kinetics | |
| JPH0368670B2 (esLanguage) | ||
| Hong et al. | Semisolid state fermentation of Baker’s yeast in an Air-Fluidized bed fermentor | |
| CN106434414A (zh) | 一种基于在线监控pH变化率的乳酸菌高细胞密度培养方法 | |
| RU2099416C1 (ru) | Способ производства хлебопекарных дрожжей | |
| US5200326A (en) | Method for the fermentative production of L-amino acids from α-keto acids | |
| HUMPHREY | Substrate utilization models for control of bioreactors | |
| SU666874A1 (ru) | Способ получени @ -лизина | |
| KR100842869B1 (ko) | 질소원 및 탄소원이 자동 공급되는 유가식 배양 방법 및이를 위한 장치 | |
| JPS6125483A (ja) | 細胞の培養方法および装置 | |
| SU1157049A1 (ru) | Способ автоматического управлени анаэробным процессом спиртового брожени | |
| CN108277238B (zh) | 一种三孢布拉霉发酵产番茄红素的方法及番茄红素 |