JPS6040854B2 - Twin containers for frozen blood production - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a container for producing frozen blood, and particularly to a container for producing frozen blood that is constructed of twin bags made of thermoplastic material.

Research has been continued into simple containers for relatively long-term blood storage in hospitals.

When blood is stored at extremely low temperatures, the metabolic activity of red blood cells is completely stopped, and the lifespan of red blood cells is significantly extended, allowing them to be stored for long periods of time. However, red blood cells undergo hemolysis when the temperature drops below -3°C, so cryopreservation of blood cells at ultra-low temperatures is by no means easier than preserving blood in a refrigerator (400). Therefore, when storing red blood cells in a moat, a cryoprotectant such as glycerol or DMSO is added to prevent freezing, and when frozen blood cells are thawed and used for transfusion, it is necessary to remove the added gag agent. There is. Therefore, a plastic bag has been proposed in which a cryoprotectant is applied to blood for transfusion aseptically and a magnetic stirrer is enclosed as a frozen blood processing device. A magnetic stirrer is enclosed in this bag, so it is possible to mix the blood cells and glycerol while rotating it on a processing device, and then put the bag directly into a deep-chilled freezer for frozen storage. be. In addition, when blood cells are thawed after cryopreservation and used for transfusion, the magnetic stirrer is used again to wash the blood cells by a dilution method using the reversible agglutination reaction of blood cells with glucose solution, and the cryoprotectant is separated and removed. This made it possible to significantly simplify the processing of frozen blood, but since this processing was carried out using a magnetic stirrer inside one bag, the bag had to be placed in a fixed position during actual operation. However, even when the supernatant is disposed of, the stirrer remains, which makes it difficult to perform centrifugation during processing. Furthermore, in the case of a plastic bag that does not contain a magnetic stirrer, the apparatus is large and expensive to perform the above-mentioned processing operations, and a large plastic bag is required, which is difficult from a practical point of view. The present invention provides a frozen blood manufacturing container (plastic bag) which eliminates the defects of the prior art described above, and its purpose is to facilitate the internal flow of blood and the injection of medical solutions. The purpose is to provide twin containers that are capable of extremely rapid thawing, and another purpose is to enclose a magnetic stirrer in one container for processing blood, drug injection, etc. To provide a twin container in which, after the operation is completed, blood can be transferred to the other container, the container containing the magnetic stirrer can be separated, and other treatments can be performed. The present invention will be described in detail below with reference to the drawings.

FIG. 1 shows an embodiment of the invention.

In the figure, 1 and 2 are twin containers (bags) made from sheets of thermoplastic material. This 2
The individual bags are connected by a connecting tube 3 having an inner diameter of 2 to 5 mm and a length of 5 to 10 mm. The capacity of the bag varies depending on the amount of frozen blood produced, but for example, the capacity of the bag is 250 [or 7
Use 00 bags (500 to 1000 bags when filled to the brim) connected together. One bag 2, for example, is filled with an electromagnet (magnetic stirrer) 4 for the internal blood rope pestle, and the other bag 1 is filled with plastic balls (beads) 5 for closing the connecting tube. The surface of the electromagnet 4 is covered with Teflon (trademark name of DuPont, USA). Each of the bags is provided with sealed openings 6, 7, 8, 9 for filling or discharging blood or necessary medical solutions, and holes 10 for hanging the bag during blood transfusion.
It has 11. It is desirable that each bag has 1 to 4 sealed entrances, and in the illustrated example, there are 2 sealed entrances.
Only pieces are shown. The sealed entrance/exit must be long enough (height) to be heat-sealable, and if each bag has multiple (4) such openings, the sealed entrance/exit must be specified in the specific application. If you do so, it will be convenient and convenient to use. Then, the entrance portion of each sealed entrance and exit port into the bag is normally closed by filling small beads (beads) 12 of a plastic material. The sheet material made of thermoplastic material may be vinyl chloride in the case of freezing at a low temperature of -80 to 110 degrees Celsius, or polyethylene, fluorine ethylene propylene polymer ( DuPont USA
Chemical Company, Inc. under the trademark Teflon FEP) and polyimide resin (commercially available from DuPont Chemical Company under the trademark Capton Type). The thickness is about 0.05~1. To serve as an enemy.

The container according to the invention performs the following processing operations in the production of frozen blood. First, blood and a medical solution are injected into the bag 2 containing the stirrer 4 from either the sealed inlet/outlet 8 or 9 through an appropriate tube, and the inner part is poured into the bag 2. By selecting an appropriate volume of the bag, this internal touhai can be performed satisfactorily.Also, by moving this bag appropriately on the external rotating electromagnet, the internal toukazusa can be performed even more effectively. When the stirrer is not built into the bag, you can vibrate the internal hawk azusa by attaching this bag to a suitable vibrator for rope azusa (shaker).Also, when electricity is not available due to a power outage, etc. can be used to apply vibration to thicken the inside. After the injection of blood and drug solution and the thickening are completed, open the beads 5 of the connecting tube 3 to the other bag 1 that does not contain the stirrer 4 and connect it. Pour the mixture of liquid and chemical solution to make the amount of silk liquid chemical inside both bags almost equal.Place both bags appropriately on top of each other or lay them flat and place them in a protective box made of metal mesh or metal plate. Let me have it built in.
It is frozen at 110qC or -200QO and thawed in warm water at 140qo when necessary, but since the thickness of the frozen solidified bag and its contents is about 1.0 to 2.3 mm, freezing and thawing are difficult. Extremely quick. Furthermore, after thawing, the blood can be transferred again to the bag 2 containing the stirrer and used for worship in the same manner as when the first drug solution was injected. of course,
It may also be vibrated manually. After injecting the necessary drug solution, move all the blood drug solution to the bag 1 that does not contain a stirrer, and close the connecting tube 3 with a heat sealer (welder or sealer) or metal cap. Then, the bag 2 containing the stirrer is separated and discarded, and the bag containing the blood drug solution is placed in a centrifuge to separate the blood.
The reason for separating and disposing of the bag (container) containing the magnetic stirrer is as follows. That is, when performing centrifugal separation when processing chemical solutions, the gravity of the magnetic stirrer will destroy the plastic bag, making it impossible to perform centrifugation.This is the greatest feature and advantage of the present invention. Yes. When repeatedly injecting and discarding chemical solutions and performing centrifugation, use the necessary number of sealed ports one by one. After each port is used, close it one by one with a heat sealer. When the operation after thawing is completed, the bag 1 contains regenerated blood and can be used for blood transfusion.As described above, the container for producing frozen blood of the present invention can be used for blood, It is easy to inject the medicinal solution, and it is possible to transfer the contents to one bag after the prescribed processing operation is completed, so the cryopreservation of blood and the blood regeneration processing operation after thawing are extremely effective. Can be done properly.

[Brief explanation of the drawing]

FIG. 1 shows an embodiment of the invention. In Figure 1, 1 and 2 are twin containers (bags), 3 is a connecting tube, 4 is an electromagnetic lamp (magnetic stirrer), 5 and 12 are beads, 6, 7, 8, and 9 are sealed entrances and exits, respectively. 10 and 11 indicate hanging holes. O diagram

Claims (1)

[Claims] 1 Consists of two plastic bags of the same size that can withstand extremely low temperatures, one bag containing a magnetic stirrer, each bag equipped with a plurality of sealed openings that can be heat-sealed, Each bag is connected by a closable connecting tube, and after blood and drug solution are injected and stirred through the sealed opening of the bag containing the magnetic stirrer, the mixed liquid is transferred to the other bag and the bag containing the magnetic stirrer is mixed. A twin container for frozen blood production, characterized in that it can be separated and centrifuged.