JPS60210984A - Activation of cellulase - Google Patents

Abstract

PURPOSE:The activation of cellulase is effected using a nonionic surfactant, polyoxyethylene phosphate ester and amphoteric surfactant, thus enabling the time of cellulase treatment to be shortened and the amount of cellulase used to be reduced. CONSTITUTION:Cellulase is activated by allowing the enzyme to exist together with at least one selected from ether-type nonionic surfactant of 13-18 HLB such as polyoxyethylene alkyl ether, polyoxyethylene phosphate ester and a carboxy-betain type amphoteric surfactant.

Description

[Detailed description of the invention] The present invention relates to a method for activating cellulase.

Since cellulose occupies 1/3 to 1/2 of the total plant mass and is produced through photosynthesis, it is considered to be the most abundant resource on earth. Cellulase is an enzyme involved in the decomposition of cellulose, and its use has been investigated in a wide range of fields, and has even been implemented in some industrial applications. For example, production of glycose from cellulose such as pulp, recovery of lignin, and waste treatment such as methane fermentation tanks.

It is used as an additive to feed, in the production of sweet bean paste, in the production of boiled beans, in peeling the endocarp of mandarin oranges, in the peeling of soybean seed coats, in the extraction of active substances from plants such as green tea components, and as a solubility promoter in disposable diapers. Furthermore, on the same date, the present applicant filed a patent application with the title of invention [Weakly Acidic Detergent Composition J] and "Weakly Acidic Detergent Composition" and proposed the use of cellulase as a detergent. In order to promote the industrial use of these cellulases or their wider and larger-scale use, it is necessary to improve the efficiency of using cellulases.

From this viewpoint, the present inventors conducted extensive searches for cellulase activators, and as a result, they came up with the present invention.

That is, the cellulase activation method of the present invention is a method of activating cellulase using at least one of the following (A) to CC) as an activating agent.

(^) Hl, former 3-18 ether type nonionic surfactant.

(B) Polyoxyethylene phosphate ester (C) Carboxybetaine type amphoteric surfactant As the ether type nonionic surfactant, one to which polyoxyethylene or polyoxypropylene is added with an ether bond is used,
For example, a first-order one can be exemplified.

(1) Polyoxyethylene alkyl (alkenyl) ether RO (CH, CI (, kO) n H R: Alkyl group or alkenyl group having 8 to 20 carbon atoms n: 12 to 30 (2) Polyoxyethylene alkylphenyl ether R : Alkyl group or alkenyl group having 8 to 20 carbon atoms n: 13 to 40 (3) Polyoxyethylene-polyoxypropylene glycol (regardless of bond formation, including random polymers, block polymers, etc.) Also, these ether type nonions It is necessary for the surfactant to have 1114B in the range of 13 to 18; if it deviates from this range, cellulase will not be activated sufficiently.By adjusting the average number of moles of hydrophobic groups and ethylene oxide added, HL Control.

Polyoxyethylene phosphate is a monoester of phosphoric acid.

1 RO(Cll, Cll, 0)n-P-DH11 R: alkyl group or alkenyl group having 8 to 20 carbon atoms,
Or an alkyl group (alkenyl group) having 8 to 20 carbon atoms (alno 1°
nyl)-nyl1. (.

n: l-15 diester, t! ) ζ1 or Tosuru Sual is a typical example, and Go, 1 Shi et al! 11 Used alone or as an ifA compound ζ.

((゛)ノJ Report, ■・Shibeta r', / type both (1゛Representative examples of surfactants, te(J).

1(.

n, Divination: an alkyl group having 1 to 20 carbon atoms or a human U xyalkyl group R2: an alkyl group having 14 to 20 carbon atoms.

This active fL agent has a concentration of 0.1~:l Owt,
It is preferable that they coexist in an amount of approximately %.

As for Reller V, who has 21 elephants in this invention.

Anything originating from living organisms such as protozoa, molluscs, bacteria, fungi, and higher species can be used.
Those originating from fungi are easily available. Regarding the organisms that are the origin of cellulases, see, for example, "Cellulases" by Shun Nishizawa (Nankodo, published in 1972), pages 21'l to 56.
etc. are listed. Cellulases are commercially available, and specific examples thereof include the following.

Cellulase AP (Cellulase AP) (manufactured by Tenshu Pharmaceutical Co., Ltd.), Cellulosin AP (COl 1ul
osin AP), Cellulosin AC (Cellulos
Ln AC) (The above is Ueda Chemical Co., Ltd.)
Driserase (Kyowa Hakko Co., Ltd.*), Cellular Zeonozuriki (Ccllul, as
e-0nozuka), pancellulase (Panc
el, 1u1. Ase), Macerozyme (manufactured by Kinki Yakuru H14 Co., Ltd.), Soluble Sucrase (
Solubl, e 5clase), Sunzyme 1
, 000 (Sanzyme 1,000) (manufactured by Tool Co., Ltd.), Cellulase A-12-C (Ce1.lu
l, ase^-12-C) (manufactured by Takeda Pharmaceutical Co., Ltd.)
, Toyo-Cellulase
(manufactured by Toyo Jozo Co., Ltd.), Cellzyme ((elluzy)
me) (Nagase Co., Ltd. W), Meiselase (Mejca
(Meiji Seika Co., Ltd. 31), Ccllucr
ust (Novo Tndust, manufactured by ry company), L
uizym (manufactured by Luipold-Werk),
Takamina-(”, ellulase(Che+
Made by aische Fabrik), Vallers
je, 1n-Ccll, ulase (Sigma Rei vJ
) Cclulase 5erva(Serva l
, ab.

-ratory +3ri, Ce1lu1. ase36(
(manufactured by Rohm and ) LAAS).

Since cellulase exhibits activity at weakly acidic conditions or lower, it is preferable to activate it in a weakly acidic region, for example, pH 3 to 6, preferably P114 to 5.5.

Activation can be easily performed by allowing the activator of the present invention to coexist with cellulase.

According to the present invention, since cellulase can be activated, cellulase treatment can be shortened and the amount of cellulase used can be reduced.

Example 0.5wt of cellulase adjusted to P115 with hydrochloric acid.

% solution, each of the activators shown in Table 1 was added at 1 νL%.
In addition, the activity of cellulase was measured by the one-bit paper disintegration method. Here, the relative activities are shown in Table 1, based on the case of cellulase alone without an activator (1,00).

Using a 1-year-old constant temperature shaker, put enzyme solution (pH 4,0) 51IIQ into a molded test tube, add 2 pieces of opening paper (IXlclll), and attach a cork stopper. 4
Shake at 0°C and 72rp+u. Measure the time required for the mouthpiece to completely disintegrate.

acid betaine

Claims (1)

[Claims] 1. An ether-type nonionic surfactant of (A) +11.1II3-18 as an activator. (b) A method for activating cellulase, which comprises using at least one selected from the group of polyoxyethylene phosphate esters and (C) carboxybetaine type amphoteric surfactants.