JPS60188169A - Serum sampling system - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION Technical Field The present invention relates to a plasma collection system, particularly a system for collecting plasma from concentrated red blood cells.

<Prior art> In recent years, the shortage of plasma has become a problem. On the other hand, concentrated red blood cells, from which most of the plasma has been removed from donor blood, are currently in oversupply, and the effectiveness of concentrated red blood cells is limited to the extent that it is used for transfusions to treat patients' red blood cell shortages and anemia. Development of usage methods is desired.

The main components of concentrated red blood cells are red blood cells, plasma, a small amount of white blood cells, and platelets, and the amount of plasma therein is approximately 2/2 to 73 m of plasma in the original blood.

It is conceivable to separate plasma from the concentrated red blood cell solution by centrifugation or membrane separation without adding physiological saline, but the yield of plasma is poor and excessive loading is required to improve the yield. Alternatively, pressure may be applied, causing destruction of blood cells, and good results may not be obtained.

Conventionally, the method used was to dilute concentrated red blood cells with physiological saline to prepare plasma, but the concentration of the collected plasma was low, and the diluted solution was 2 to 3 times that of the original plasma. Efficacy and efficiency were unsatisfactory.

Purpose> In view of the above, an object of the present invention is to provide a continuous system that can efficiently collect plasma or concentrated plasma from a concentrated red blood cell solution.

<Example> The present invention involves diluting a concentrated red blood cell solution with physiological saline (hereinafter referred to as saline) as a diluting solution, separating the diluted concentrated red blood cell solution into a residual blood cell solution and a liquid component using a model separator, and dissolving the liquid component. This method collects plasma or concentrated plasma by concentrating it using a model concentrator.

The model separator is a hollow fiber type or small and easy to handle, and membrane materials such as cellulose triacetate, cellulose diacetate, polyvinyl alcohol, polymethyl methacrylate, polyethylene, polymer alloy, and polypropylene are used. The following is an example.

Hollow fiber membrane material: Cellulose triacetate-1, hollow fiber inner diameter: 270μm Hollow fiber membrane thickness: 80μtM Effective membrane area: 0.5m', 0.8mm Effective length:
163mm, 220 theory hollow fiber largest hole diameter 20.4μ? 2
1. For the above-mentioned reasons, the model concentrator is also preferably of the hollow fiber type, and cellulose triacetate, polyacrylonitrile, polymethyl methacrylate, polysulfone, etc. are used as the membrane material. Examples of the concentrator include the following.

Hollow fiber membrane material: cellulose triacetate hollow fiber inner diameter:
200μη2 Hollow fiber membrane thickness: 25μm Effective membrane area: 1.8 n? Effective length: 220 Pharyngeal fraction molecular weight: Approximately 30,000 (albumin).

Globrine transmission) Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 shows the flow path system of the first embodiment, in which 1 is a concentrated liquid line, 2 is a liquid component line, 3 is a plasma line, 4 is a diluted liquid line, and 5 is a blood cell residual liquid line. line,
6 is the filtrate line, 7 is the blood pump, 8 is the diluent pump,
9 is a liquid component pump, 10 is a model separator, 11 is a model concentrator, 12 is a concentrated liquid bag, 13 is a diluted liquid bag, 14 is a residual blood cell bag, 15 is a plasma bag, 16 is a reactor liquid bag, 17 is a The pressure regulating clamp 18 is a heater.Next, the configuration and operation of the system of the present invention will be explained.

The concentrated liquid red blood cells contained in the concentrated liquid bag 12 are transported to the model separator 1o via the concentrated liquid line 1 which communicates with the bag and is equipped with a blood pump 7. The saline serving as a diluent in the bag is pumped into the concentrated liquid line 1 through a diluent line 4 which is connected to the concentrated liquid line 1 and equipped with a diluent pump 8, and the red blood cell concentrate is diluted. is heated to about 37° C. by a heater 18 disposed in the thick ffi line 1 in order to facilitate filtration in the model separator 1o and model concentrator 11 by increasing fluidity.

The diluted red blood cell concentrate transported to the model separator 1o was filtered through a porous membrane in the separator to be composed of residual blood cells as a red blood cell concentrate with reduced plasma content, plasma, and saline. The residual blood cell fluid is stored in the blood cell residual fluid bag 14 via the blood cell residual fluid line 5, and the residual fluid can be used as a red blood cell suspension after post-processing. It is pumped to a model concentrator 11 via a liquid component line 2 equipped with a liquid component pump 9.

The liquid components are membrane-filtered in the model separator 11 and separated into plasma or concentrated plasma and saline as a P solution or saline containing plasma electrolytes, and the plasma or concentrated plasma is collected into a plasma bag 15 through a plasma line 3, The liquid passes through the furnace liquid line 6 and is stored in the P liquid chamber and the knob 16, but the filtration concentration ratio is controlled by the operation of the pressure regulating clamp 17 installed in the plasma line 3 to control the liquid component pressure in the molded concentrator 11. Plasma can be obtained when the p-liquid flow rate is made equal to the above-mentioned saline flow rate, and concentrated plasma can be obtained when the p-liquid flow rate is made larger than the saline flow rate.

In Figure 2, the diluent bag 13 is omitted and the diluent line 4 is
is connected to the p-liquid bag 16 to form a communication line] 9, which enables a loop circuit configuration by the communication line 19, the concentrated liquid line 1, the liquid component line 2, and the furnace liquid line, and the loop If the circuit is previously filled with saline as a diluted solution, when the system starts operating, the saline in the loop circuit is transported to the concentrated liquid line 1, where it is separated and concentrated in exactly the same way as in the previous example, and the pressure is adjusted. When the flow rate of liquid F in the furnace liquid line G is made larger than the flow rate of the diluent pump 8 by controlling the clamp 17, the flow rate of lachrymal fluid for concentration is greater than the flow rate of the diluent, and the liquid is concentrated in the plasma bag 15. Plasma is collected and the amount of furnace liquid in the V5 liquid bag 16 gradually increases, and when the meteors are equalized, plasma with a normal concentration is obtained, but in this case, the Pl + liquid bag 16 and the pressure regulating clamp I7 are omitted. Directly connect P liquid line 6 and communication line] 9 to operate only diluent pump 8. 7
The M% concentration flow rate and the F' liquid flow rate can be made equal, simplifying the plasma collection operation and saving the diluent bag.

FIG. 3 shows a system in which the pressure regulating clamp 17 is omitted from the system shown in FIG. 2, and a p liquid pump 20 is installed in the reactor liquid line 6. When collecting concentrated plasma, the pressure regulating clamp is used to control the filtration pressure. Since the filtration concentration ratio is determined from the flow rate difference with the diluent pump 8 by setting the flow rate of the pump, plasma concentration can be achieved without the need to measure the flow rate of the slurry line 6 in the first and second embodiments. It is easy to control the ratio, and as in the case of the first embodiment, when collecting plasma at a normal concentration, the p liquid bag 16 and the F5 liquid pump 20 are omitted, and the furnace liquid line 6 and the communication line 19 are directly connected. Furthermore, in the first embodiment, the IP liquid pump 20 having the arrangement shown in FIG.
can be adopted.

In FIG. 3, a branch line 21 shown by a chain line is provided which communicates the liquid component line 2 upstream of the liquid component pump 9 and the plasma bag 15.
plasma bag 1 using the loop circuit formed between
The plasma in 5 can be recirculated through the circuit together with the liquid components from the demolding separator 10, which recirculation allows for an increase in the total flow rate in the demolding concentrator at a given liquid component flow rate. This shows the effect of preventing clogging of the membrane in the concentrator at low flow rates, and the other operations are the same as in the previous explanation, and the loop circuit constituted by the branch line 21 is the same as in the previous embodiment. It is also applicable to

Next, to show an example of the operating conditions of an actual plasma collection system, in the system shown in Fig. 3, the membrane areas of 0.8 yn'' and 18 mm, which were illustrated earlier as the demolding separator and model concentrator, respectively, are used. When used, red blood cell concentrate flow rate 9
0m11min, diluent flow rate 30m1Zmin, liquid component flow rate 48
In 77 minutes, 14.4 ynl of concentrated plasma could be continuously collected under conditions of a reactor liquid pump flow rate of 33.6 m and 67 minutes.

<Effects> In the present invention, diluted red blood cell concentrate is pumped to a demolding separator, and the liquid components separated in the separator are concentrated in a model concentrator to collect plasma or concentrated plasma. Highly efficient continuous operation is possible and plasma of the desired concentration can be obtained.

[Brief explanation of the drawing]

The drawings show embodiments of the present invention, and FIGS. 1 to 3 are flow path diagrams of different embodiments, in which 7 is a blood pump, 8 is a diluent pump, 9 is a liquid component pump, 10 is a demolding separator, 11 is a model concentrator, 12 is a concentrated liquid bag,
15 is a plasma bag, 17 is a pressure regulating clamp, 19 is a communication line, 20 is a reactor liquid pump, and 21 is a branch line. Patent applicant Nilasho Co., Ltd. - “Jvl team 2 Seki 9

Claims (1)

[Scope of Claims] ■ Concentrated red blood cells continuously diluted with a diluent are pumped to a demolding separator, the sputum components filtered in the separator are pumped to a model concentrator, and the concentrator 1. A plasma collection system comprising: a plasma or concentrated plasma obtained by ρ-filtration concentration of a liquid component in the plasma collection system; (2) The plasma collection system according to claim 1, wherein the furnace fluid flow rate control means is a pressure regulating clamp installed on a plasma line that draws plasma from the model concentrator. ■ The P liquid line that takes out the filtered reactor liquid in the model concentrator is connected to the diluent line for diluting the red blood cell concentrate to form a communication line, and the communication line is used to remove the diluted red blood cell concentrate from the mold. A diluent is filled in advance in a loop circuit constituted by a concentrated liquid line for transporting to a separator and a liquid component line from the separator to a model concentrator,
2. The plasma collection system according to claim 1, wherein the reactor liquid flow rate control means is a diluent pump for continuously diluting the red blood cell concentrate. ■ A loop circuit filled with a dilution liquid in advance as described in claim 3 is provided, a furnace liquid container capable of storing liquid is interposed in the communication line constituting the circuit, and the furnace liquid flow rate control means is provided as claimed in claim 3. The plasma collection system according to claim 1, which is a pressure regulating clamp according to claim 2. (2) The plasma collection system according to claim 1, wherein the furnace liquid flow rate control means is a furnace liquid pump disposed in a filtrate line for drawing out the filtrate from the model concentrator. ■ The plasma according to claim 5, characterized in that the furnace liquid line equipped with a furnace liquid pump constitutes the loop circuit according to claim 3 via a p-liquid container capable of storing liquid. Collection system. ■ The plasma container connected to the plasma line for drawing plasma from the model concentrator and the liquid component line for drawing the liquid component from the model separator are connected by a branch line, and the plasma in the plasma container is connected to the plasma line for drawing the plasma from the model separator. 7. The plasma collection system according to claim 1, wherein the plasma collection system is recirculated together with the liquid component through a model concentrator.