JP3368683B2 - Blood component separation method - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for separating each blood component in whole blood using a blood bag. [0002] A blood bag is used when various blood components contained in whole blood are taken out and formulated. As such a blood bag, there is a blood bag set shown in Japanese Patent Publication No. 63-20144. This is equipped with a plasma collection connection at the upper end of the parent bag from which whole blood is collected,
The lower end of the parent bag is provided with a concentrating red blood cell collecting connection portion, and the plasma collecting child bag and the concentrated red blood cell collecting child bag are connected to the upper connecting portion and the lower connecting portion via an upper tube and a lower tube, respectively. Yes. When the whole blood collected in the parent bag is separated for each blood component, the whole blood bag set is placed in a centrifuge and the whole blood inside the parent bag is centrifuged. As a result of the centrifugation, a plasma layer is formed in the upper part inside the parent bag, a thin layer (buffy coat) containing white blood cells is formed in the middle part, and a layer containing red blood cells is formed in the lower part. Thereafter, the parent bag is squeezed by a blood separation squeezing device, and each blood component in the upper and lower parts inside the parent bag is passed through each connection part and each tube, and the plasma collection child bag and the concentrated red blood cell collection child Simultaneously transferred to the bag. The blood bag set described above is very advantageous in terms of labor saving and shortening of working time because the blood components in the upper layer and the lower layer in the parent bag can be simultaneously transferred to two child bags. By the way, when the blood bag set is centrifuged by the centrifuge, the blood bag set is set in the centrifuge so that the tube does not contact the main body of each bag. However, since the blood bag set is provided with a connecting portion at the lower end portion, and a tube is connected to the connecting portion, the tube at the lower end portion of the bag is unavoidable when the blood bag set is set in the centrifuge. Is easy to set with the upper and lower sides of the main bag body in contact. When set in this way, the side surface of the parent bag is strongly pressed against the external tube when it is centrifuged, and the periphery of the pressed portion of the parent bag swells to form a protrusion. In particular, the lower portion of the main body of the main bag is prone to be formed with protrusions because of a greater gravitational acceleration during centrifugation. When such a protrusion is formed, there is a problem that the protrusion formed on the parent bag is easily damaged. In order to solve this problem, a blood bag set as shown in FIG. 3 has been developed. The blood bag set of FIG. 3 has a parent bag 1 and a blood collection needle 3 with a blood collection tube 2, a plasma collection child bag 4, a concentrated red blood cell collection child bag 5, and tubes 6 and 7 attached to the top and bottom of the parent bag. The parent bag 1 collects whole blood and is mainly composed of a main body 8. The main body 8 is composed of two sheets made of synthetic resin, and the periphery of these sheets is bonded to form a bag. Yes. Blood collection tube 2 at the upper end of body 8
Are connected to each other, a connecting part 9 for plasma transfer, a connecting part 11 for transporting concentrated erythrocytes, and a blood transfusion port 13 with a pair of protectors 12. The plasma transfer connector 10 and the concentrated erythrocyte transfer connector 11 are sandwiched and fixed between the two sheets when the peripheral edges of the two sheets of the main body 8 are bonded. Communication pieces 14 and 15 are provided that allow the inside and outside of the main body 8 to communicate with each other by bending inside the plasma transfer connection portion 10 and the concentrated red blood cell transfer connection portion 11. The lower end portion of the plasma transfer connection portion 10 opens at the upper end portion inside the main body 8, and the lower end portion of the concentrated red blood cell transfer connection portion 11 opens at the lower end portion inside the main body 8, Opposing through a small gap. The plasma collection child bag 4 and the concentrated erythrocyte collection child bag 5 are connected to the plasma transfer connection portion 10 of the main body 8 so that the blood components in the upper and lower parts of the main body 8 can be transferred.
・ The tube 6 is connected to the connection part 11 for transporting the concentrated erythrocyte, respectively.
7 is connected. When the blood bag set as described above is used, first, the blood collection tube 2 and the connecting portion 9 are drawn from the blood collection needle 3.
The whole blood is introduced into the main body 8 of the parent bag 1 from the blood donor. When the blood bag set is placed in a centrifuge and the blood inside the main body 8 is centrifuged, the main body 8
An upper layer containing plasma is separately formed in the upper part, a buffy coat layer is formed in the middle part, and a lower layer containing red blood cells is formed in the lower part. After centrifuging whole blood as described above, the end of the blood collection tube 2 on the side of the connection portion 9 is shut off, and then the communication pieces 14 and 15 are bent and folded so that the inside of the main body of the parent bag 1 is
The plasma collection child bag 4 and the concentrated erythrocyte collection child bag 5 are communicated with each other through the connecting portions 10 and 11 and the tubes 6 and 7, and plasma and concentrated erythrocytes are simultaneously transferred. When the blood bag set described above is used, the lower end portion of the concentrated red blood cell transfer connection portion 11 is opened at the lower end portion inside the main body 8, while the lower end portion inside the main body 8 is small. Since they face each other through a gap, blood enters the connecting portion 11 when blood is collected in the main bag body 8. When the blood in the connection part 11 is centrifuged in this state, the connection part 1
1 is separated into each layer of plasma, buffy coat, and concentrated erythrocytes. The fact that plasma / buffy coat is contained in the connecting portion 11 means that concentrated erythrocytes with high purity cannot be obtained when concentrated erythrocytes are transferred to a child bag. The present invention has been made for the purpose of collecting blood components of high-purity plasma and concentrated erythrocytes as compared with the case obtained by the conventional method. In the present invention, a blood collection needle is connected to each part opened at the upper end of the parent bag with a tube, and further, a plasma collection child bag and a concentrated red blood cell collection. Using a synthetic resin blood bag set, each connected by a tube containing a communication piece that allows the child bag to communicate by bending, (1) blood is collected in the parent bag and centrifuged to separate the plasma portion and buffy Operation to divide into coat / rich erythrocyte layer, (2) Two side-by-side from the point connected to the concentrated red blood cell collection child bag at the upper end of the parent bag toward the lower end of the parent bag The operation of squeezing from the outside of the parent bag with the moved hand, (3) The two moving hands are opposed to each other while being pushed toward the peripheral edge of the main bag body, and between the two moving hands. And in the parent bag Operation to form a passage for collecting concentrated erythrocytes (4)
By communicating the inside of the parent bag and each child bag, and then pressurizing the blood in the parent bag, each layer of the plasma portion and the concentrated red blood cell portion is transferred to each child bag, leaving only the buffy coat in the parent bag. The gist of the blood component separation method is characterized by comprising operations. According to the present invention, since the blood bag set is made of synthetic resin, each blood component separated into each layer inside the parent bag can be obtained by simply devising the compressed state outside the parent bag. It can be transferred to a child bag. The blood bag set employed in the present invention is:
A blood collection needle is connected to each part of the connection portion opened at the upper end of the parent bag with a tube, and a plasma collection child bag and a concentrated erythrocyte collection child bag are connected in a state where they can communicate with each other through the tube. Here, the state in which communication is possible means that the inside and outside of the parent bag are bent by folding inside the connection part, that is,
It is provided with the communication piece (it is also called a folding bar) which connects the inside of a parent bag and the inside of a child bag. Such blood bag sets are well known. In the present invention, it is also a well-known operation to divide blood collected in the parent bag into a plasma part, a buffy coat, and a concentrated red blood cell part by centrifugation. In the present invention, 2 is the second operation.
The operation of squeezing from the outside of the parent bag with the moving hands arranged side by side is to make blood as little as possible present at the location being squeezed within the bag body. A moving hand is provided from the position connected to the child bag for collecting concentrated red blood cells at the upper end of the parent bag toward the lower end of the parent bag. It is for hitting. In the present invention, there is a third operation (3) for forming a concentrated red blood cell collection passage in the parent bag.
The moving hand of the book is pushed toward the peripheral edge of the main bag body. This passage preferably has a cross-sectional area that is the same as or slightly larger than the inner area of the communication tube. The reason for pushing in the movement of the moving hand is to prevent the buffy coat or plasma from entering the passage. Also, when transferring blood components, the lower end of the moving hand must extend below the buffy coat layer so that the concentrated red blood cells do not mix with buffy coat or plasma. For these operations, it is preferable that the moving hand has a rod shape and is in surface contact with the bag body. In the present invention, there is a fourth operation for transferring blood components to the child bag. In order that each blood component can be transferred even if the blood in the parent bag is compressed, the communication piece in the communication tube is bent and folded, for example, so that the inside of the parent bag and each child bag are communicated in advance. Also at this time, the bag body portion where the moving hand is located remains pressed. In the present invention, the plasma portion from the parent bag
When each layer of the concentrated red blood cell portion is transferred to each child bag and only the buffy coat remains in the parent bag, it may be managed visually or may be optically managed such as a photo sensor. Further, when a conventional blood bag set is applied to a centrifuge, the main bag and the tube of each bag come into contact with each other and the parent bag is easily damaged. However, if the blood bag set employed in the present invention is used, Since all the tubes are connected to the upper end of the bag, there is little possibility that the parent bag will be damaged. Embodiments of the present invention will now be described in detail with reference to the drawings. FIG. 1 is a plan view of a blood bag set employed in the present invention, and FIG. 2 is a plan view of a blood bag set for explaining the method of the present invention. The blood bag set of FIG. 1 has a parent bag 1, a blood collection tube 2 with a blood collection needle 3, a plasma collection child bag 4, a concentrated red blood cell collection child bag 5, and tubes 6 and 7 attached on the parent bag. The parent bag 1 collects whole blood and is mainly composed of a main body 8. The main body 8 is composed of two sheets made of synthetic resin such as polyvinyl chloride, and the periphery of these sheets is heated. Bonded by fusion to form a bag. A connecting portion 9 to which the blood collection tube 2 is connected to the upper end of the main body 8, a connecting portion 10 for transferring plasma, a connecting portion 11 for transferring concentrated red blood cells,
A blood transfusion port 13 with a pair of protectors 12 is provided. The plasma transfer connector 10 and the concentrated erythrocyte transfer connector 11 are composed of a soft tube made of synthetic resin such as polyvinyl chloride, and both are bonded when the peripheral edges of the two sheets of the main body 8 are bonded. It is fixed between the sheets.
Communication pieces 14 and 15 are provided for connecting the inside of the main body 8 to the outside, that is, the inside of the main body 8 and the inside of the child bags 4 and 5 by bending inside the connecting part 10 for transferring plasma and the connecting part 11 for transferring concentrated red blood cells. ing. Both the lower end portion of the plasma transfer connection portion 10 and the lower end portion of the concentrated red blood cell transfer connection portion 11 are opened at the upper end portion inside the main body 8. A blood preservation solution such as CPD solution or ACD-A solution is enclosed in the main body 8, and the concentrated red blood cell collection child bag 5 is mainly composed of mannitol and adenine, which can extend the effective period of red blood cells to 42 days. An erythrocyte preservation solution as a component, so-called MAP solution, or the like is enclosed. The plasma collection child bag 4 and the concentrated erythrocyte collection child bag 5 are connected to the plasma transfer connection portion 10 of the main body 8 so that the blood components in the upper and lower parts inside the main body 8 can be transferred after centrifugation. -It is connected to the concentrated red blood cell transfer connection part 11 via tubes 6 and 7, respectively. When the blood bag set as described above is used, first, the blood collection tube 2 and the connection portion 9 are drawn from the blood collection needle 3.
The whole blood is introduced into the main body 8 of the parent bag 1 from the blood donor. Then, when the blood bag set is put in a centrifuge and the blood inside the main body 8 is centrifuged at about 4000 rpm, the upper layer containing plasma is formed in the upper part of the main body 8, the buffy coat 16 is formed in the middle part, and the lower part A lower layer containing red blood cells is formed separately. After centrifuging whole blood as described above, FIG.
As shown in FIG. 4, the parent bag 1
Squeezing from the outside of the parent bag 1 with the moving hands 17 and 18 toward the vicinity of the lower end portion of the parent bag 1 from the portion connected toward the concentrated red blood cell collection child bag 5 at the upper end portion of the bag. As a result, the two films forming the main body 8 are pressure-bonded only at that point. Next, the two moving hands 17 and 18 are pushed toward the peripheral edge of the main body of the parent bag while being separated so that the two are opposed in parallel. In FIG. 2, the moving hand 17 is pushed to the left as viewed in the figure, and the moving hand 18 is pushed to the right as viewed in the figure. The lower end of the concentrated erythrocyte collection passage (not shown) in the parent bag thus formed is located below the buffy coat layer 16. The communication pieces 14 and 15 are folded and the connection part 1
By connecting the inside of the parent bag 1 and the inside of each child bag 4, 5 through the tubes 0, 11 and the tubes 6, 7, the blood in the parent bag 1 is compressed, so that the layer of the thick red blood cell portion is moved by the hand 1
The layer of the plasma part is also transferred to the child bag 4 at the same time through the lower part of the concentrated red blood cell collection passage formed at 7 and 18 to the child bag 5. Then, only the buffy coat 16 is left in the parent bag 1. When it is desired to enlarge the concentrated red blood cell collection passage, the passage portions of the two films forming the main body 8 must be loosened, and the distance between the opposed moving hands 17 and 18 needs to be slightly closer prior to the transfer of each blood component. There is. As a result of separating blood components by the method of the present invention,
93% of the red blood cells in the parent bag 1 could be transferred to the concentrated red blood cell collection child bag 5, and 80% of the white blood cells present in the parent bag 1 could be left in the parent bag 1. As a comparative example, as a result of centrifuging blood components by the method shown in FIG.
% Of the leukocytes existing in the parent bag 1 can be transferred to the concentrated red blood cell collection child bag 5.
Only 1% remained in the parent bag 1. As described above, by separating blood components according to the present invention, it is possible to obtain a preparation of concentrated erythrocytes and plasma containing few leukocytes.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a plan view of a blood bag set employed in the present invention. FIG. 2 is a plan view of a blood bag set for explaining the method of the present invention. FIG. 3 is a plan view of a conventional blood bag set. [Description of Symbols] 1 Parent bag 2 Blood collection tube 3 Blood collection needle 4 Plasma collection child bag 5 Concentrated red blood cell collection child bag 6 Tube 7 Tube 8 Body 9 Connection 10 Plasma transfer connection 11 Concentration red blood cell connection 12 Protector 13 Blood transfusion port 14 Communication piece 15 Communication piece 16 Buffy coat 17 Moving hand 18 Moving hand

──────────────────────────────────────────────────── ─── Continued from front page (58) Fields surveyed (Int.Cl. ⁷ , DB name) A61J 3/00 300 A61K 35/14 A61M 1/02 525

Claims (1)

(57) [Claims] [Claim 1] A blood collection needle is connected to each portion opened at the upper end of the parent bag with a tube, and further, a plasma collection child bag and a concentrated erythrocyte collection child bag However, using a blood bag set made of synthetic resin, each connected with a tube containing a communication piece that communicates by bending and folding,
(1) Blood is collected in a parent bag and is separated into a plasma part, a buffy coat, and a concentrated red blood cell part by centrifugation, and (2) is connected to the concentrated red blood cell collection child bag at the upper end of the parent bag. Pressing from the outside of the parent bag with two moving hands arranged side by side toward the lower end of the parent bag, (3) pushing the two moving hands toward the peripheral edge of the main body of the parent bag An operation of forming a concentrated red blood cell collection passage between the two moving hands and in the parent bag, and (4) communicating between the parent bag and each child bag. The blood component separation is characterized in that, by pressing the blood in the parent bag, each layer of the plasma portion and the concentrated red blood cell portion is transferred to each child bag, and only the buffy coat remains in the parent bag. Method.