JPH0678993A - Method for separation and blood bag set - Google Patents

Abstract

PURPOSE:To provide a blood bag set capable of power saving and shortening of working time and having no possibility of leucocyte being mixed into the erythrocyte separated into a lower slave bag by providing such a constitution that the lower end opening of a lower connecting part is situated in an upper layer after centrifugal separation, and situated in a lower layer after transfer of the upper layer. CONSTITUTION:A blood bag set has a parent bag 1 and upper and lower slave bags 4, 5. A full-blood sample is collected, and guided into the body 8 of the parent bag 1. When the blood bag set is then put in a centrifugal separator and centrifugally separated, an upper layer 17 containing blood plasma is separately formed in the upper part in the body 8, a thin middle layer 18 containing leucocyte and blood platelet in the middle part, and a lower layer 19 containing erythrocyte in the lower part. When the body 8 of the parent bag 1 is squeezed to transfer the blood component of the upper layer 17 to the upper slave bag 4, the lower end opening of the lower connecting part 13 is situated in the lower layer 19. When the body 8 of the parent bag 1 is similarly squeezed to transfer the blood component of the lower layer 19 to the lower slave bag 5.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood separation method and a blood bag set used in this method.

[0002]

2. Description of the Related Art When various blood components contained in a whole blood sample are taken out and formulated, blood components are separated using, for example, a blood bag set. An example of a blood separation method using a blood bag set is shown in US Pat. No. 5,102,407, in which a whole blood sample of a blood bag set is collected as shown in FIG. At the upper end of the parent bag 31, the parent bag 3
The upper connection part 32 that opens at the upper end inside 1 and the lower connection part 33 that is inserted into the inside of the parent bag 31 and that opens at the lower end thereof are provided. The upper connection part 32 and the lower connection part 33 include: Upper child bag 34 and lower child bag 35
Are connected to each other via the upper tube 36 and the lower tube 37 (in the illustrated example, the lower connecting portion 3).
3 and the lower tube 37 are integrated. ).

When separating the whole blood sample collected in the parent bag 31 into blood components, the whole blood bag set is placed in a centrifuge and the whole blood sample in the parent bag 31 is centrifuged. . This allows the parent bag 31
An upper layer 38 containing plasma is provided in the upper part of the interior, and a thin intermediate layer containing white blood cells (buffy coat) is provided in the middle part.
39, and a lower layer 40 containing red blood cells is separately formed in the lower portion. After that, the parent bag 31 is squeezed, and the upper layer 38 inside the parent bag 31 becomes the upper connecting portion 3
2 and the upper tube 36 through the upper tube 3
After being transferred to 4, the lower layer 40 inside the parent bag 31
It is transferred to the lower child bag 35 via the lower connecting portion 33 and the lower tube 37. The above blood separation method is
The blood components in the upper part and the lower part inside the parent bag 31 can be easily and quickly transferred to the two child bags 34, 35, which is advantageous in terms of labor saving and shortening of working time.

[0004]

However, in the above-mentioned prior art, the lower connecting portion 33 is provided at the upper end portion of the parent bag 31, and is inserted to the lower end inside the parent bag 31 and opened at that position. Therefore, the whole blood sample collected in the parent bag 31 enters the lower connection part 33, and at the time of centrifugation, the whole blood sample in the lower connection part 33 is centrifuged similarly to the outside. A thin intermediate layer 39 containing white blood cells was formed in the central portion in the vertical direction. Therefore, when the lower layer 40 containing red blood cells in the parent bag 31 is transferred into the lower child bag 35 via the lower connecting portion 33 and the lower tube 37, white blood cells are contained in the lower child bag 35. There was a problem that was mixed. The present invention is to solve the above problems, and is advantageous in terms of labor saving and shortening of working time, and in the red blood cells separated in the lower child bag, the blood separation without the risk of white blood cells being mixed. An object is to provide a method and a blood bag set.

[0005]

In order to solve the above-mentioned problems, the blood separating method of the present invention is characterized in that an upper connecting portion and a lower connecting portion are provided in an upper portion and an upper portion is provided in an upper portion inside. A whole blood sample is collected in a parent bag into which the connecting portion for insertion is opened, and the whole blood sample is contained in the upper layer containing plasma and the lower end opening of the connecting portion for lower portion and white blood cells. After centrifuging into an intermediate layer and a lower layer containing red blood cells and transferring the upper layer to an upper child bag through an upper connecting portion, a lower connecting portion in which a lower end opening is located in the lower layer is transferred. Then, the lower layer is transferred to the lower child bag. In addition, a feature of the blood bag set of the present invention is that a parent bag from which a whole blood sample is collected has an upper child bag and a lower child bag,
Connected via the upper and lower connections, the whole blood sample inside the parent bag is centrifuged into an upper layer containing plasma, an intermediate layer containing white blood cells, and a lower layer containing red blood cells. Then, the upper layer, via the upper connection,
In the upper child bag and the lower layer transferred to the lower child bag through the lower connecting portion, the upper bag has an upper connecting portion and a lower connecting portion, respectively. The upper connection part opens at the upper part inside the parent bag, and the lower connection part is inserted into the upper part inside the parent bag,
The lower end opening is at a point located in the upper layer after the centrifugation and in the lower layer after the transfer of the upper layer.

[0006]

When the whole blood sample is sampled and centrifuged, the whole blood sample is first introduced into the parent bag. Next, when the blood bag set is put into a centrifuge and the blood inside the parent bag is centrifuged, the upper layer inside the parent bag contains an upper layer containing plasma and the middle part contains white blood cells. A thin middle layer (buffy coat) is at the bottom,
A lower layer containing red blood cells is formed separately, and the lower end opening of the lower connection portion is located in the upper layer. After that, the parent bag is squeezed, and the blood component in the upper layer inside the parent bag is transferred to the upper child bag via the upper connecting portion and the upper tube.

After transferring the upper layer, the intermediate layer and the lower layer remain inside the parent bag, but the lower connecting portion passes through the intermediate layer, and the lower end opening of the lower connecting portion is below the intermediate layer. Located in a layer. Next, the parent bag is squeezed in the same manner as described above, and the blood component of the lower layer inside the parent bag is transferred to the lower child bag via the lower connecting portion and the lower tube. In the above case, when the whole blood sample is introduced into the parent bag, the whole blood sample enters the lower connection part, but the lower end opening of the lower connection part is located in the upper layer after centrifugation. Since it is located above the intermediate layer containing white blood cells, a thin intermediate layer containing white blood cells is not formed in the lower connection portion during centrifugation, like the outside thereof. Therefore, when the lower layer containing red blood cells in the parent bag is transferred to the lower child bag as described above, there is no fear that white blood cells will be mixed in the lower child bag.

[0008]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings of FIGS. Figure 1 shows a blood bag set,
It has a parent bag 1, a blood collection needle 3 with a blood collection tube 2, upper / lower child bags 4 and 5, upper and lower tubes 6 and 7, and the like. The parent bag 1 is used for collecting a whole blood sample, and is mainly composed of a main body 8 as shown in FIGS. 2 and 3, and the main body 8 is made of two sheets of synthetic resin such as polyvinyl chloride. And the peripheries of these sheets are adhered. At the upper end of the main body 8, a connecting portion 9 to which the blood collection tube 2 is connected and a pair of protectors 10 are provided.
With blood transfusion port 11 and upper and lower connection parts 12, 13
Is provided.

In the embodiment, the upper and lower connecting portions 12 and 13 are made of, for example, a soft tube made of synthetic resin such as polyvinyl chloride, and are used to bond the two sheets of the main body 8 together. , It is sandwiched between both seats and fixed. The upper part of the upper and lower connecting portions 12, 13 is the main body 8
Of the communicating piece 1 that projects upward from the inside of the body 8 and connects the inside and outside of the body 8 by bending at the top.
4, 15 are provided. The upper connecting portion 12 is the main body 8
It opens at the upper end inside, and its lower end opening is at the same position as the upper end inside the main body 8 in the vertical direction. The lower connecting portion 13 is inserted inside the main body 8 and opens at the upper portion inside the main body 8.

Inside the main body 8, CPD liquid, ACD-
A blood preservation solution such as solution A is enclosed. The upper and lower child bags 4 and 5 are for transferring the respective blood components in the upper and lower portions inside the main body 8, respectively.
It is connected to the lower connecting portions 12 and 13 via the upper and lower tubes 6 and 7. According to the embodiment configured as described above, when collecting and centrifuging a whole blood sample, first, the whole blood is collected from the blood donor from the blood collecting needle 3 via the blood collecting tube 2 and the connecting portion 9. The sample is introduced into the main body 8 of the parent bag 1.

Then, the blood bag set is placed in a centrifuge, and the blood inside the main body 8 is centrifuged at 4540 × g for 6 minutes. As a result, as shown in FIG. The upper layer 17 containing contains a thin intermediate layer (buffy coat) containing white blood cells and platelets in the middle part.
18 and a lower layer 19 containing red blood cells is separately formed in the lower portion, and the lower end opening of the lower connecting portion 13 is located in the upper layer 17. Next, the connecting portion 9 of the blood collection tube 2
After shutting off the side end, the communication piece 14 is bent to separate the upper part inside the main body 8 of the parent bag 1 and the upper child bag 4.
The upper connection portion 12 and the upper tube 6 are communicated with each other.

Then, the main body 8 of the parent bag 1 is
The blood component of the upper layer 17 inside the main body 8 is squeezed by the squeezing device for blood separation and transferred to the upper child bag 4 via the upper connecting portion 12 and the upper tube 6. Upper layer 1
After transferring 7, the intermediate layer 18 and the lower layer 19 remain inside the main body 8 of the parent bag 1 as shown in FIG. 3, but the lower connecting portion 13 passes through the intermediate layer 18 and its lower end. The opening is located in the lower layer 19 below the intermediate layer 18.
Next, after shutting off the end portion of the upper tube 6 on the side of the upper connection portion 12, the communication piece 15 is bent to form the parent bag 1
The lower layer 19 inside the main body 8 and the upper child bag 4 are connected via the upper connecting portion 12 and the upper tube 6.

Then, the main body 8 of the parent bag 1 is squeezed in the same manner as described above, and the blood component of the lower layer 19 inside the main body 8 is passed through the lower connecting portion 13 and the lower tube 7 to form the lower child bag. Transfer to 5. In this way, the blood components in the upper part and the lower part inside the main body 8 are quickly transferred to the two child bags 4,
5 can be easily transferred, which is advantageous in terms of labor saving and shortening of working time.

Further, in the above case, when the whole blood sample is introduced into the main body 8 of the parent bag 1, the whole blood sample enters the lower connecting portion 13, but the lower end opening of the lower connecting portion 13 is opened. Are located in the upper layer 17 after centrifugation and above the intermediate layer 18 containing white blood cells and platelets, and therefore, during centrifugation, in the lower connection portion 13, like the outside thereof, And a thin intermediate layer containing platelets is not formed. Therefore, when the lower layer 19 containing red blood cells in the parent bag 1 is transferred to the lower child bag 5 as described above, there is no fear that white blood cells and platelets are mixed in the lower child bag 5. In the examples, centrifugation was performed with a large centrifugal force of 4540 × g so that the intermediate layer contained platelets. However, the centrifugal force was reduced to
Sometimes the upper layer contains platelets.
Further, in the embodiment, the connecting portions for upper and lower portions are made of tubes, but these connecting portions may be formed integrally with the main body of the parent bag. You may integrate with the tube for each. Further, in the embodiment, each of the upper and lower connecting portions is addressed to one, but a plurality of them may be addressed.

[0015]

As described in detail above, according to the present invention,
In the work of extracting the blood component from the whole blood sample, it is advantageous in terms of labor saving and shortening of the working time, and there is no fear that the white blood cells are mixed with the red blood cells separated in the lower child bag.

[Brief description of drawings]

FIG. 1 is a front view of a blood bag set showing an embodiment of the present invention.

FIG. 2 is a front view of a main part showing an embodiment of the present invention.

FIG. 3 is a front view of a main part showing an embodiment of the present invention.

FIG. 4 is a front view of a blood bag set showing a conventional example.

[Explanation of symbols]

 1 Parent Bag 4 Upper Child Bag 5 Lower Child Bag 6 Upper Tube 7 Lower Tube 8 Main Body 12 Upper Connecting Portion 13 Lower Connecting Portion 17 Upper Layer 18 Middle Layer 19 Lower Layer

Claims (2)

[Claims] 1. A whole blood sample is collected in a parent bag having an upper connection part and a lower connection part in the upper part and having an upper connection part inserted in the upper part inside to open, and the whole blood sample is collected. Is centrifuged into an upper layer containing plasma and in which a lower end opening of the lower connection portion is located, an intermediate layer containing white blood cells, and a lower layer containing red blood cells, and the upper layer is connected via the upper connection portion. The method for separating blood is characterized in that the lower layer is transferred to the upper child bag and then the lower layer is transferred to the lower child bag via the lower connecting portion in which the lower end opening is located in the lower layer. 2. An upper bag and a lower bag are connected to a parent bag from which a whole blood sample is collected via an upper connecting portion and a lower connecting portion, respectively, and the whole blood inside the parent bag is connected. The sample is centrifuged into an upper layer containing plasma, an intermediate layer containing white blood cells, and a lower layer containing red blood cells, and the upper layer is connected via the upper connection to the upper child bag, In the case where the lower layer is transferred to the lower child bag through the lower connecting portion, the upper bag has an upper connecting portion and a lower connecting portion, and the upper connecting portion has an upper connecting portion. Is opened in the upper part inside the parent bag, the lower connection part is inserted in the upper part inside the parent bag, and the lower end opening is located in the upper layer after the centrifugation and the lower part is transferred after the transfer of the upper layer. Blood bag set characterized by being located in layers .